Nucleic acid aptamers specifically combined with beta-amyloid precursor protein lyase 1 and application of aptamers

Abstract

The invention discloses nucleic acid aptamers specifically combined with beta-amyloid precursor protein lyase 1 and an application of the nucleic acid aptamers. The nucleotide sequences of the nucleic acid aptamers are any one of SEQ ID NO: 1 and SEQ ID NO: 2. The nucleic acid aptamers or chemical modifiers of the nucleic acid aptamers serving as BACE1 inhibitors can be applied to preparing drugs for treating Alzheimer's disease. The DNA aptamers A1 and A4 which can be combined with BACE1 with high affinity are successfully screened by virtue of an SELEX technology in the research, and the aptamers have the effect of inhibiting the in vitro activity of the BACE1. The nucleic acid aptamers are used for providing a research basis on development of novel potent and specific BACE1 inhibitors and providing novel methods and concepts to treatment of AD.

Description

Technical field: [0001] The invention belongs to the field of biomedicine, and specifically relates to a nucleic acid aptamer specifically combined with β-amyloid precursor protein lyase 1 and an application thereof. Background technique: [0002] Alzheimer's disease (AD) is a progressive degenerative disease of the central nervous system, with cognitive dysfunction as the main clinical manifestation, and other symptoms including executive dysfunction and mental disorders. Advanced patients cannot take care of themselves and often die from complications such as lung infection; it brings huge economic burden to the patient's family and society [1] . It is predicted that the number of AD patients worldwide will triple from 36 million in 2010 to 2050 [2] . The cause of AD is unknown. At present, it is believed that a variety of pathogenic factors, such as amyloid precursor protein / amyloidβ (APP / Aβ) [3] , Apolipoprotein E4 (ApolipoproteinE4, apoE4) [4,5] 、tau [6,7] , α-sy...

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