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A kind of hplc method to measure the method of L-cysteine ​​content

A technology of cysteine ​​and content, which is applied in the field of high performance liquid chromatography, can solve the problems of special excipients interference, etc., and achieve the effect of ensuring quality control, ensuring stability and improving symmetry

Active Publication Date: 2016-08-17
WUHAN GRAND HOYO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the HPLC method for measuring L-cysteine ​​has been reported in the literature, because of the interference of special auxiliary materials in the product, a method for measuring the content of L-cysteine ​​in L-cysteine ​​capsules by HPLC was invented. Method, searched, no report

Method used

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  • A kind of hplc method to measure the method of L-cysteine ​​content
  • A kind of hplc method to measure the method of L-cysteine ​​content
  • A kind of hplc method to measure the method of L-cysteine ​​content

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Instruments and Conditions

[0032] Chromatographic column: normal phase silica gel bonded diol column;

[0033] Mobile phase: acetonitrile-ammonium dihydrogen phosphate aqueous solution (pH2.0) (75:25);

[0034] Column temperature: 30°C;

[0035] Flow rate: 1.0ml / min;

[0036] Injection volume: 20μl;

[0037] Detection wavelength: 200nm;

[0038] Experimental procedure

[0039] Take 20 mg of L-cysteine ​​reference substance, accurately weigh it, put it in a 100ml measuring bottle, dissolve it with a buffer solution with a pH value of 1.2 and dilute to the mark, shake it well, and use it as the reference substance solution. Take 20 samples, remove the capsule shell, get the content, grind it finely with a mortar, take 80mg of fine powder (equivalent to 20mg of L-cysteine), weigh it accurately, put it in a 100ml measuring bottle, add 50ml of it with a pH value of 1.2 Shake the buffer solution for 15 minutes on a fast mixer, dilute to the mark with a buffer solution...

Embodiment 2

[0042] Instruments and Conditions

[0043] Chromatographic column: normal phase silica gel bonded diol column;

[0044] Mobile phase: acetonitrile-ammonium dihydrogen phosphate aqueous solution (pH2.0) (80:20);

[0045] Column temperature: 30°C;

[0046] Flow rate: 1.0ml / min;

[0047] Injection volume: 20μl;

[0048] Detection wavelength: 200nm;

[0049] Experimental procedure

[0050] Take 20 mg of L-cysteine ​​reference substance, accurately weigh it, put it in a 100ml measuring bottle, dissolve it with a buffer solution with a pH value of 1.2 and dilute to the mark, shake it well, and use it as the reference substance solution. Take 20 samples, remove the capsule shell, get the content, grind it finely with a mortar, take 80mg of fine powder (equivalent to 20mg of L-cysteine), weigh it accurately, put it in a 100ml measuring bottle, add 50ml of it with a pH value of 1.2 Shake the buffer solution for 15 minutes on a fast mixer, dilute to the mark with a buffer solution...

Embodiment 3

[0053] Instruments and Conditions

[0054] Chromatographic column: normal phase silica gel bonded diol column;

[0055] Mobile phase: acetonitrile-ammonium dihydrogen phosphate aqueous solution (pH2.0) (85:15);

[0056] Column temperature: 30°C;

[0057] Flow rate: 1.0ml / min;

[0058] Injection volume: 20μl;

[0059] Detection wavelength: 200nm;

[0060] Experimental procedure

[0061] Take 20 mg of L-cysteine ​​reference substance, accurately weigh it, put it in a 100ml measuring bottle, dissolve it with a buffer solution with a pH value of 1.2 and dilute to the mark, shake it well, and use it as the reference substance solution. Take 20 samples, remove the capsule shell, get the content, grind it finely with a mortar, take 80mg of fine powder (equivalent to 20mg of L-cysteine), weigh it accurately, put it in a 100ml measuring bottle, add 50ml of it with a pH value of 1.2 Shake the buffer solution for 15 minutes on a fast mixer, dilute to the mark with a buffer solution...

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Abstract

The invention discloses a method for measuring the L-cysteine content of an L-cysteine capsule by an HPLC (high performance liquid chromatography) process. According to the method, the L-cysteine content is measured through the chromatography process taking a mixed solvent as a flowing phase by a normal-phase silica gel bonded diol chromatographic column. Through the method, the L-cysteine content of a sample can be quickly measured.

Description

technical field [0001] The invention relates to a high-performance liquid chromatography, in particular to a high-performance liquid chromatography for analyzing the content of L-cysteine ​​in L-cysteine ​​capsules. Background technique [0002] L-cysteine ​​is an amino acid with physiological functions. It is the only amino acid with a reducing group sulfhydryl (-SH) among more than 20 amino acids that make up proteins. The molecular formula is C 3 h 7 NO 2 S, the molecular weight is 121.16, the chemical name is L-2-amino-3-mercaptopropionic acid, and the structural formula is [0003] [0004] L-cysteine ​​has been widely used in medicine, food additives and cosmetics because of its extensive physiological functions. The traditional determination of L-cysteine ​​is carried out by ultraviolet spectrophotometry, but due to the interference of excipients and other problems, the content of L-cysteine ​​cannot be accurately and quickly determined, and the product quality ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 李慧乔春莲冯小路周静杨波
Owner WUHAN GRAND HOYO