A kind of assay method of sodium cholate content

A determination method, the technology of sodium cholate, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of composition and content quantification, and achieve the effect of accurate results, wide application and strong promotion

CN104458930BActive Publication Date: 2016-08-17WUHAN BIOCHEM PHARMA
1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2016-08-17

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for measuring the content of sodium cholate. The method comprises the following steps: (1) preparing a sodium cholate solution to be measured; (2) respectively preparing a standard hyodesoxycholic acid solution and a standard chenodeoxycholic acid solution; and (3) measuring the contents of hyodesoxycholic acid and chenodeoxycholic acid which are contained in the sodium cholate solution to be measured by adopting a high efficiency liquid chromatography method. The method disclosed by the invention has the characteristics of easiness and convenience for operation, accuracy in result, short measurement time and high generalizability and can be widely applied to the technical field of component detection.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for detecting components, in particular to a method for determining the content of sodium cholate. Background technique

[0002] Sodium cholate is a mixture of bile salts extracted from pig bile, and it is clinically used as a choleretic medicine for treating cholecystitis, bile deficiency and other diseases. In addition to a large amount of water, pig bile mainly contains bile salts, bile pigments, cholesterol, fatty acids, lecithin and some inorganic salts, and bile salts are potassium salts of bile acids formed by combining various bile acids with glycine or taurine or sodium salt form. After extraction and purification, the main component of sodium cholate is bile acid sodium salt, which is a mixture of glycine, taurine and various bile acids combined with bile acid sodium salt.

[0003] "Famous Doctors" records: the main chemical components in bile are bile acids, bile pigments, mucin, lipids and inorganic subst...

Examples

Embodiment Construction

[0026] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, but these embodiments should not be construed as limiting the present invention.

[0027] A method for assaying sodium cholate content, comprising the steps of:

[0028] (1) Prepare the sodium cholate solution to be tested: (1.1) Take 0.5 g of sodium cholate produced by Wuhan Biochemical Pharmaceutical Co., Ltd., accurately weigh it and place it in a flask, add 30 ml of 15% sodium hydroxide solution and 5 ml of ethanol, After heating to boiling, reflux through the condenser tube for 6 hours, rinse the condenser tube with 30ml of water, filter, and place the lotion in a separatory funnel, wash the flask and the separatory funnel twice with 5ml hot water, and combine the lotion into the flask middle.

[0029] (1.2) Add sulfuric acid dropwise to the reflux liquid to make the pH value 2.7, and let it cool.

[0030] (1.3) The reflux liquid in the f...