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A suspension culture method for producing American ginseng saponins

A technology of American ginseng saponins and suspension culture, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc. It can solve the problems of non-belonging and inability to "direct saponins" and achieve stable production, reliable methods, and easy control.

Inactive Publication Date: 2017-05-03
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, American ginseng fruit fermentation method is also used to produce or enrich the total saponins of American ginseng, which mainly uses the action of enzymes produced during fermentation by yeasts. This method can increase the amount of total saponins, but it does not belong to the true sense. "produce", and the method cannot "target" the production of saponins

Method used

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  • A suspension culture method for producing American ginseng saponins
  • A suspension culture method for producing American ginseng saponins
  • A suspension culture method for producing American ginseng saponins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Materials

[0029] 1.1. Formula of induction medium:

[0030] Add 1.5mg / L of 2,4-D and 0.5mg / L of 6-BA to the MS basic medium, adjust the pH to 4.5-5.8 before sterilization, and fill in 150mL Erlenmeyer flasks, each bottle is filled with 30mL , sterilization;

[0031] 1.2. Formula of subculture medium:

[0032] Add 1.5mg / L of 2,4-D and 0.5mg / L of 6-BA to the MS basal medium, adjust the sucrose concentration to 20g / L, adjust the pH to 4.5-5.8 before sterilization, and use a 150mL Erlenmeyer flask Divide into 30mL per bottle and sterilize;

[0033] 1.3. The formula of the suspension medium:

[0034] Add 0.8mg / L of 2,4-D, 0.1mg / L of kinetin KT, 0.3mg / L of 6-BA, 0.3mg / L of NAA to the MS basal medium, adjust the sucrose concentration to 20g / L , before sterilization, adjust the pH to 4.5-5.8, fill in 150mL Erlenmeyer flasks, fill each bottle with 30mL, and sterilize.

[0035] 2. Method

[0036] (1) Disinfection: take the root tip of the main root of the American gin...

Embodiment 2

[0049] In this embodiment, the culture medium formula is:

[0050] 1) The formula of induction medium:

[0051] Add 2.5mg / L of 2,4-D and 1.0mg / L of 6-BA to the MS basic medium, adjust the pH to 4.5~5.8 before sterilization, use 150mL Erlenmeyer flasks, and fill each bottle 30mL, sterilized;

[0052] 2) The formula of subculture medium:

[0053] Add 2.5mg / L of 2,4-D and 1.0mg / L of 6-BA to MS basal medium, adjust the sucrose concentration to 30g / L, adjust the pH to 5.8 before sterilization, and pack in 150mL Erlenmeyer flasks , 30mL per bottle, sterilized;

[0054] 3) The formula of the suspension medium:

[0055] Add 1.0mg / L of 2,4-D, 0.1mg / L of kinetin KT, 0.5mg / L of 6-BA, 0.5mg / L of NAA in the MS basal medium, adjust the sucrose concentration to 30g / L , before sterilization, adjust the pH to 4.5-5.8, fill in 150mL Erlenmeyer flasks, fill each bottle with 30mL, and sterilize.

[0056] 4) The formula of the suspension culture medium of total saponin directional culture is...

Embodiment 3

[0066] In this embodiment, the culture medium formula is:

[0067] 1) The formula of induction medium:

[0068] Add 2.0mg / L 2,4-D and 1.0mg / L 6-BA to the MS basic medium, adjust the pH to 4.5-5.8 before sterilization, use 150mL Erlenmeyer flasks, and fill each bottle 30mL, sterilized;

[0069] 2) The formula of subculture medium:

[0070] Add 2.0mg / L of 2,4-D and 1.0mg / L of 6-BA to the MS basal medium, adjust the sucrose concentration to 25g / L, adjust the pH to 4.5-5.8 before sterilization, and use a 150mL Erlenmeyer flask Divide into 30mL per bottle and sterilize;

[0071] 3) The formula of the suspension medium:

[0072] Add 0.9mg / L of 2,4-D, 0.1mg / L of kinetin KT, 0.4mg / L of 6-BA, 0.4mg / L of NAA to the MS basal medium, adjust the sucrose concentration to 25g / L , before sterilization, adjust the pH to 4.5-5.8, fill in 150mL Erlenmeyer flasks, fill each bottle with 30mL, and sterilize.

[0073] 4) The formula of the suspension culture medium of total saponin directional...

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Abstract

The invention discloses a suspension culture method for producing quiquefolium saponins and in particular discloses American ginseng root tip callus induction culture, subculture and suspension culture related factors and combined ratios thereof. Compared with the prior art, the method disclosed by the invention has the advantages that the condition that the content of saponin is changed due to change of culture conditions is avoided, so that the panaxoside can be stably produced; and meanwhile, the method disclosed by the invention is simple to operate, easy to control and reliable, the culture conditions are controlled, directional breeding of total saponins and saponin monomer can be realized, theoretical basis and technical guidance can be provided for industrial production of the quiquefolium saponins, and the method has profound significance for deep exploitation and utilization of resources.

Description

technical field [0001] The invention relates to the field of natural products, in particular to a suspension culture method for producing American ginseng saponins. Background technique [0002] American ginseng (Panax quinquefolium) is a perennial herb of the Araliaceae genus Panax. American ginseng is an ancient plant growing in the primeval forests of North America. It is known as a "living fossil" and is a precious Chinese medicine with high medicinal and economic value. Since the successful introduction of American ginseng in a large area in the 1980s, my country has developed into the world's third Atlantic ginseng producer and the world's largest consumer after the United States and Canada. American ginseng is bitter in taste and cool in nature. It enters the heart, lungs and kidney meridian. The methods of taking are divided into boiling, stewing, steaming, slicing and melting, grinding into fine powder and taking after mixing. American ginseng contains rich biolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 丁之恩孙雨薇丁昱魏芬陆小雨袁艳
Owner ANHUI AGRICULTURAL UNIVERSITY
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