A gilbert syndrome gene mutation detection kit

A detection kit and syndrome technology, applied in the field of Gilbert syndrome gene mutation detection kits, can solve the problems of expensive sequencing and other problems

Active Publication Date: 2017-12-29
吴旭平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, PCR sequencing technology is mainly used to detect the mutation of Gilbert syndrome gene, but the sequencing price is relatively expensive, so it is necessary to find a technology with simple application, reliable results and low price to develop the Gilbert syndrome gene detection kit

Method used

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  • A gilbert syndrome gene mutation detection kit
  • A gilbert syndrome gene mutation detection kit
  • A gilbert syndrome gene mutation detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Gilbert syndrome gene mutation site detection kit and its use

[0064] 1. Prepare a kit including the components in the following table:

[0065] serial number

product composition

main ingredient

20-person ration

1

PBREM PCR reaction solution

Buffer, MgCl2, dNTPs, Taq enzyme, PBREM primer, etc.

700μl × 1 tube

2

TA211 PCR reaction solution

Buffer, MgCl2, dNTPs, Taq enzyme, TA211 primer

700μl×2 tubes

3

Positive Control 1

UGT1A1 enhancer element (PBREM) gene fragment

300μl×1 tube

4

Positive Control 2

UGT1A1 promoter region fragment and exon 1 gene fragment

300μl × 2 tubes

5

30% acrylamide

Acrylamide

60μl × 1 tube

6

50% glycerol

Glycerol

200μl×1 tube

7

10% ammonium persulfate

Ammonium persulfate

60μl × 1 tube

[0066] The PBREM primer sequences in the above table are: upstream primer: 5’-CAACTCATCCTTATTCTGAT-3’; dow...

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Abstract

The invention relates to a gene mutation detection kit for the GS (Gilbert syndrome) and belongs to the technical field of biology. The kit comprises DNA (deoxyribose nucleic acid) micro-array chips formed by fixing an acrylamide modified PCR (polymerase chain reaction) product and other components on a propylene group modified slide as well as 6 nucleotide probe parts. The kit adopts a DNA micro-array technology, is simple and convenient to operate, low in price and capable of being applied to screening and clinical auxiliary diagnosis of diseases related to the GS caused by gene mutation.

Description

technical field [0001] The invention relates to a gene detection kit, in particular to a Gilbert syndrome gene mutation detection kit, which belongs to the field of biotechnology. Background technique [0002] Gilbert syndrome (Gilbert syndrome, GS), first reported by Gilbert and Lereboullet in 1901, also known as constitutional dyshepatic jaundice, is a common hereditary unconjugated bilirubinemia. Patients with this disease have jaundice due to the dysfunction of hepatic uptake of unconjugated bilirubin (unconjugated bilirubin, UCB) and the insufficiency of glucuronosyltransferase (uridine diphosphate glucuronosyl transferase, UGT) in microsomes, resulting in increased blood UCB. Patients mainly present with non-hemolytic intermittent jaundice mainly caused by elevated indirect bilirubin, and some patients may experience fatigue, dyspepsia, liver discomfort, and fat intolerance. [0003] With the development of molecular biology techniques, the research on Gilbert syndrom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C40B50/18
CPCC12Q1/6837C12Q2565/518
Inventor 吴旭平宋金云孙梅王建杨永峰周东蕊
Owner 吴旭平
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