Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A gilbert syndrome gene mutation detection kit

A detection kit and syndrome technology, applied in the field of Gilbert syndrome gene mutation detection kits, can solve the problems of expensive sequencing and other problems

Active Publication Date: 2017-12-29
吴旭平
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, PCR sequencing technology is mainly used to detect the mutation of Gilbert syndrome gene, but the sequencing price is relatively expensive, so it is necessary to find a technology with simple application, reliable results and low price to develop the Gilbert syndrome gene detection kit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A gilbert syndrome gene mutation detection kit
  • A gilbert syndrome gene mutation detection kit
  • A gilbert syndrome gene mutation detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Gilbert syndrome gene mutation site detection kit and its use

[0064] 1. Prepare a kit including the components in the following table:

[0065] serial number

product composition

main ingredient

20-person ration

1

PBREM PCR reaction solution

Buffer, MgCl2, dNTPs, Taq enzyme, PBREM primer, etc.

700μl × 1 tube

2

TA211 PCR reaction solution

Buffer, MgCl2, dNTPs, Taq enzyme, TA211 primer

700μl×2 tubes

3

Positive Control 1

UGT1A1 enhancer element (PBREM) gene fragment

300μl×1 tube

4

Positive Control 2

UGT1A1 promoter region fragment and exon 1 gene fragment

300μl × 2 tubes

5

30% acrylamide

Acrylamide

60μl × 1 tube

6

50% glycerol

Glycerol

200μl×1 tube

7

10% ammonium persulfate

Ammonium persulfate

60μl × 1 tube

[0066] The PBREM primer sequences in the above table are: upstream primer: 5’-CAACTCATCCTTATTCTGAT-3’; dow...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
fluorescenceaaaaaaaaaa
Login to View More

Abstract

The invention relates to a gene mutation detection kit for the GS (Gilbert syndrome) and belongs to the technical field of biology. The kit comprises DNA (deoxyribose nucleic acid) micro-array chips formed by fixing an acrylamide modified PCR (polymerase chain reaction) product and other components on a propylene group modified slide as well as 6 nucleotide probe parts. The kit adopts a DNA micro-array technology, is simple and convenient to operate, low in price and capable of being applied to screening and clinical auxiliary diagnosis of diseases related to the GS caused by gene mutation.

Description

technical field [0001] The invention relates to a gene detection kit, in particular to a Gilbert syndrome gene mutation detection kit, which belongs to the field of biotechnology. Background technique [0002] Gilbert syndrome (Gilbert syndrome, GS), first reported by Gilbert and Lereboullet in 1901, also known as constitutional dyshepatic jaundice, is a common hereditary unconjugated bilirubinemia. Patients with this disease have jaundice due to the dysfunction of hepatic uptake of unconjugated bilirubin (unconjugated bilirubin, UCB) and the insufficiency of glucuronosyltransferase (uridine diphosphate glucuronosyl transferase, UGT) in microsomes, resulting in increased blood UCB. Patients mainly present with non-hemolytic intermittent jaundice mainly caused by elevated indirect bilirubin, and some patients may experience fatigue, dyspepsia, liver discomfort, and fat intolerance. [0003] With the development of molecular biology techniques, the research on Gilbert syndrom...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C40B50/18
CPCC12Q1/6837C12Q2565/518
Inventor 吴旭平宋金云孙梅王建杨永峰周东蕊
Owner 吴旭平
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com