Application of Mycobacterium tuberculosis antigenic protein rv1798 and its T cell epitope peptide

Abstract

The invention relates to mycobacterium tuberculosis antigen protein Rv1798 and application of a T cell epitope peptide of the mycobacterium tuberculosis antigen protein Rv1798 in preparation of tuberculosis detection reagents, vaccines and drugs. An amino acid sequence of the mycobacterium tuberculosis protein antigen Rv1798 and an amino acid sequence of the T cell epitope peptide of the protein antigen Rv1798 are shown in SEQ ID NO:1-3 respectively. The mycobacterium tuberculosis protein antigen Rv1798 and the T cell epitope peptide thereof are applied to a specific T cell and B cell immune response caused by mycobacterium tuberculosis by serving as irritants; and when the mycobacterium tuberculosis protein antigen Rv1798 and the T cell epitope peptide thereof are applied to the specific T cell and B cell immune response caused by the mycobacterium tuberculosis by serving as the irritants, the condition that a false positive result is caused due the fact that an antigen is not pure can be reduced compared with an original method that a complete antigen is adopted. The detection reagents prepared from the protein antigen Rv1798 and the epitope peptide thereof can be widely applied to the related fields of assistant diagnosis of tuberculosis, epidemiological monitoring and the like. The tuberculosis vaccines and the antituberculous drugs which are prepared from the protein antigen Rv1798 can be used for preventing and treating the tuberculosis.

Description

Technical field [0001] The present invention relates to the fields of molecular biology and immunology, in particular to the application of Mycobacterium tuberculosis antigen protein Rv1798 and its T cell epitope peptide in the preparation of tuberculosis detection reagents, vaccines and medicines. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis. The survey results show that one third of the world's population is latently infected, and 5% to 10% may develop into active tuberculosis in future life. Since the World Health Organization declared tuberculosis a global crisis in 1993, the morbidity and mortality of tuberculosis have remained high. According to the WHO report, about 8 million new tuberculosis patients are added every year, and about 2 to 3 million people die from tuberculosis every year. my country ranks second among 22 countries with a high burden of tuberculosis. The results of the fifth national epidemio...

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Problems solved by technology

However, due to the high requirements for the bacterial content in the sample, the sensitivity of this method is not high, resulting in a large number of smear-negative patients being undetected and turning positive, and smear-negative patients are contagious, which cannot be ignored. This method has no species specificity and poor sensitivity , affected by sputum specimen and disease condition

Sputum culture is the gold standard for the diagnosis of tuberculosis, but the culture time is too long. Now there are rapid culture systems such as the BACTEC MGIT960 system that can isolate and culture Mycobacterium tuberculosis within 2 weeks. Bacterial inhibitors are expensive and cannot be widely promoted in developing countries, and the contamination rate of rapid culture is significantly higher than that of improved L-J culture, resulting in false positive results

The commonly used detection method for population screening is the skin tuberculin test, which uses pure protein derivatives of Mycobacterium tuberculosis (PPD), because PPD contains many mycobacterial species (pathogenic mycobacteria, environmental mycobacteria bacillus and BCG), so the specificity of PPD diagnosis of tuberculosis is poor, and it cannot effectively distinguish between Mycobacterium tuberculosis infection and BCG vaccination. Tuberculosis imaging examinations include routine X-ray examination, CT examination, MRI examination, Ultrasound examinations are expensive, cause certain harm to the body, have low specificity, and are not suitable for routine examination and diagnosis

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