A method and application of reducing the conservation algebra of silkworm germplasm resources without diapause polymorphism

Abstract

The invention discloses a method for reducing conservation generations of non-diapause multivoltine silkworm germplasm resource and an application of the method. The method comprises the steps of protecting spawn non-diapause multivoltine silkworm germplasm resource silkworm eggs for 38-42 hours in an environment with a temperature of 25-26 DEG C and a relative humidity of 75-80%, transferring the eggs into an environment with a temperature of 14-16 DEG C and keeping for 1.5-2.5 hours, then transferring the eggs into an environment with a temperature of 1-10 DEG C for refrigeration storage for 25-28 days, transferring the eggs to an environment with a temperature of 14-16 DEG C for keeping until the silkworm egg embryos develop to the body pigmentation stage, taking out, conducting blackening treatment in an environment with a temperature of 27-28 DEG C and a humidity of 80-85% to urge the silkworm egg embryos to smoothly develop until incubation, and gathering newly hatched silkworms and breeding. The method can prolong the refrigeration storage period of the non-diapause multivoltine silkworm breeds by 20-22 days. The conservation generations of non-diapause multivoltine silkworm germplasm resource is reduced to 4 generations from 7-8 generations characterized by the traditional method, thus greatly reducing the breeding times and alleviating labor intensity.

Description

technical field

[0001] The invention belongs to the field of preservation of silkworm germplasm resources, and in particular relates to a method and application for reducing the number of conservation generations of silkworm germplasm resources without diapause and polymorphism. Background technique

[0002] According to the number of generations that occur each year, silkworm germplasm resources can be divided into monophyletic, dimorphic, and polymorphic, among which polymorphic silkworm germplasm can be further divided into diapause polymorphism and non-diapause There are two types of diversification.

[0003] The non-diapause polymorphic silkworm germplasm resource is a precious biological germplasm resource in nature, an important part of the existing silkworm germplasm resource in my country, and also the original material for silkworm genetic breeding and basic scientific research. At present, there are more than 40 germplasm resources of non-diapause polymorphic sil...

Examples

Embodiment 1

[0028] A method for reducing the number of conservation generations of non-diapause polymorphic silkworm germplasm resources, comprising the steps of:

[0029] (1) Non-diapause polymorphic silkworm germplasm resources Silkworm eggs are protected for 40 hours in an environment with a temperature of 25-26° C. and a relative humidity of 75%-80% after laying.

[0030] (2) Then move to an environment at 15°C for 2 hours;

[0031] (3) Then move to a 5°C environment for refrigerated storage for 28 days;

[0032] (4) Move it to an environment of 15°C and place it until the silkworm egg embryo develops to the greening stage; 305 and Qiongshan Hainan need 29 days, Taisha and 7018 need 28 days, and 301 and P50 need 27 days.

[0033] (5) Take out, and carry out blackout treatment under the environment of temperature 27~28 ℃, humidity 80%~85%, promote the smooth development of silkworm egg embryo to hatch, collect ants normally and raise.

[0034] (6) Analyze the preservation effect, and...

technical field

[0001] The invention belongs to the field of preservation of silkworm germplasm resources, and in particular relates to a method and application for reducing the number of conservation generations of silkworm germplasm resources without diapause and polymorphism. Background technique

[0002] According to the number of generations that occur each year, silkworm germplasm resources can be divided into monophyletic, dimorphic, and polymorphic, among which polymorphic silkworm germplasm can be further divided into diapause polymorphism and non-diapause There are two types of diversification.

[0003] The non-diapause polymorphic silkworm germplasm resource is a precious biological germplasm resource in nature, an important part of the existing silkworm germplasm resource in my country, and also the original material for silkworm genetic breeding and basic scientific research. At present, there are more than 40 germplasm resources of non-diapause polymorphic sil...

Examples

Embodiment 1

[0028] A method for reducing the number of conservation generations of non-diapause polymorphic silkworm germplasm resources, comprising the steps of:

[0029] (1) Non-diapause polymorphic silkworm germplasm resources Silkworm eggs are protected for 40 hours in an environment with a temperature of 25-26° C. and a relative humidity of 75%-80% after laying.

[0030] (2) Then move to an environment at 15°C for 2 hours;

[0031] (3) Then move to a 5°C environment for refrigerated storage for 28 days;

[0032] (4) Move it to an environment of 15°C and place it until the silkworm egg embryo develops to the greening stage; 305 and Qiongshan Hainan need 29 days, Taisha and 7018 need 28 days, and 301 and P50 need 27 days.

[0033] (5) Take out, and carry out blackout treatment under the environment of temperature 27~28 ℃, humidity 80%~85%, promote the smooth development of silkworm egg embryo to hatch, collect ants normally and raise.

[0034] (6) Analyze the preservation effect, and...

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