Determination of Physiology and Biochemistry of Mosses Polluted by Polybrominated Diphenyl Ethers and Heavy Metals

A polybrominated diphenyl ether, physiological and biochemical technology, applied in the environmental field, can solve problems such as unclear atmospheric monitoring

Inactive Publication Date: 2019-07-16
QUZHOU UNIV +1
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] At present, the toxic mechanism of polybrominated diphenyl ethers (PBDEs) and heavy metal pollution to moss and the atmospheric monitoring of moss to such pollution are still unclear

Method used

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  • Determination of Physiology and Biochemistry of Mosses Polluted by Polybrominated Diphenyl Ethers and Heavy Metals
  • Determination of Physiology and Biochemistry of Mosses Polluted by Polybrominated Diphenyl Ethers and Heavy Metals
  • Determination of Physiology and Biochemistry of Mosses Polluted by Polybrominated Diphenyl Ethers and Heavy Metals

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Embodiment Construction

[0077] The following are specific embodiments of the present invention and in conjunction with the accompanying drawings, the technical solutions of the present invention are further described, but the present invention is not limited to these embodiments.

[0078]The method for measuring the physiology and biochemistry of mosses under the pollution of polybrominated diphenyl ethers and heavy metals, selected the more common mosses, Physcomitr grandis and Physcomitrella acupoints as the test objects, in the presence of heavy metal Pb and two kinds of polybrominated diphenyl ethers BDE-47 (representing low bromine Class PBDEs), BDE-209 (representing high bromine PBDEs), wherein, BDE-209 solution is prepared by BDE-209 powder not dissolved in dimethyl sulfoxide organic solution, the BDE- The 47 solution is prepared as a mother liquor by BDE-47 powder undissolved in dimethyl sulfoxide organic solution. BDE-47, BDE-209 mother liquor preparation: Dissolve 200mg of BDE-47 powder and...

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Abstract

The invention provides a poly brominated diphenyl ether and heavy metal pollution moss physiological and biochemical determining method, and belongs to the technical field of environment. The poly brominated diphenyl ether and heavy metal pollution moss physiological and biochemical determining method comprises the following steps of (1) selecting a reagent; (2) selecting and culturing the moss; (3) performing virus exposure on the moss; (4) culturing the moss into an artificial climate box; (5) putting the cultured moss into a refrigerator being -80 DEG C to be stored; (6) performing repeated treatment for three times; determining chlorophyll (Chl), malonaldehyde (MDA), proline (Pro), superoxide radicals, (O2<->), hydrogen peroxide (H2O2), superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in each group of moss. The method has the advantages that the structure is simple; the moss physiological and biochemical features are obvious, so that the features can be better used as markers for atmospheric pollution early warning.

Description

technical field [0001] The invention belongs to the field of environmental technology, and relates to a method for measuring the physiology and biochemistry of mosses, in particular to a method for measuring the physiology and biochemistry of mosses under the pollution of polybrominated diphenyl ethers and heavy metals. Background technique [0002] Moss is a plant group that transitions from aquatic to terrestrial, and is an important category of higher plants. Its unique physiological and ecological characteristics have obvious advantages in monitoring air pollution: ①It can grow and reproduce in extremely harsh environments; ②Bryophytes absorb water and nutrients from the air through body surface exchange; ③Pollutants can easily pass through the cell wall directly on the leaf surface; ④The cell wall is thick, with strong cation exchange capacity and metal element retention; ⑤Meristem After the plant is dormant or dead, it can also develop rapidly to keep it evergreen all ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 赵建华林匡飞陆强
Owner QUZHOU UNIV
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