Method for detecting illegally added ribavirin in health care product

Abstract

The invention discloses a method for detecting illegally added ribavirin in a health care product. The method comprises the following steps: a phenolphthalein-ethanol solution, a sodium hydroxide solution, tert-butyl diphenyl chlorosilane, a pyridine aqueous solution, a catalyst DMAP aqueous solution, chloroform, a hydrochloric acid solution and excessive calcium oxide are added into a sample to be detected, wet litmus test paper is used, and whether the sample contains ribavirin is determined according to twice chromogenic reaction. All used reagents are common chemical reagents, the identification method is sensitive to reaction, high in specificity and obvious in phenomena, large instruments are not needed, operation is simple and safe, the cost is lot, and the method is suitable for large-batch onsite rapid detection. The detection limit of illegally added ribavirin in the health care product is 1.0 mg / time.

Description

technical field

[0001] The invention relates to the detection field, in particular to a detection method for illegally adding ribavirin in health products. Background technique

[0002] The chemical name of ribavirin is 1-β-D-ribofuranose-1,2,4-triazole-3-hydroxyamide, which is a broad-spectrum and potent antiviral drug, mainly used to treat various viral infections Diseases are currently widely used in the prevention and treatment of common viral diseases. Common dosage forms include injections, tablets, oral liquids, aerosols, etc. The reported adverse reactions of ribavirin include allergic reactions, anaphylactic shock, blood system reactions, nervous system reactions, circulatory system reactions, damage to the skin and mucous membranes and their accessories, teratogenicity, and fetal death. Therefore, ribavirin should be taken under the guidance of a physician, and the indications and contraindications should be clarified and used reasonably.

[0003] However, in or...

Examples

Embodiment 1

[0031] The concentrated hydrochloric acid in this example is the analytically pure concentrated hydrochloric acid supplied by Nanjing Chemical Reagent Co., Ltd., the analytically pure acetic acid supplied by Xilong Science Co., Ltd. for acetic acid, and the analytically pure anhydrous sodium acetate supplied by Sinopharm Chemical Reagent Co., Ltd. For sodium acetate and phenolphthalein, select analytically pure phenolphthalein supplied by Xilong Science Co., Ltd., for ethanol, select analytically pure absolute ethanol supplied by Xilong Science Co., Ltd., and for sodium hydroxide, select analytically pure sodium hydroxide supplied by Sinopharm Chemical Reagent Co., Ltd. For chloroform, select analytically pure chloroform supplied by Nanjing Chemical Reagent Co., Ltd., for calcium oxide, select analytically pure calcium oxide supplied by Nanjing Chemical Reagent Co., Ltd., and for tert-butyldiphenylchlorosilane (TBDPSCL), select Alfa Aesar ( Analytical pure tert-butyldimethylchl...

Embodiment 2

[0042] A method for detecting illegally added ribavirin in health products, comprising the following steps:

[0043] Step a: Take a sample of 1 capsule, take out the contents of the capsule, grind it, add it to 3ml of water, shake it to dissolve, let it stand still, and prepare a sample solution;

[0044]Step b: Slowly add 0.01ml of phenolphthalein ethanol solution to the sample solution, oscillate and shake well, slowly drop into 0.03ml0.01% sodium hydroxide solution, oscillate and shake well to obtain a mixed solution, observe the color change of the mixed solution, Carry out the first judgment, after dripping the 0.01% sodium hydroxide solution, the mixed solution turns red, after shaking and shaking, it is observed that the red color of the mixed solution does not disappear, and it is judged that there is no ribavirin in the sample , terminate the detection;

[0045] The sample solution is colorless after adding phenolphthalein ethanol solution, indicating that the sample...

Embodiment 3

[0047] A method for detecting illegally added ribavirin in health products, comprising the following steps:

[0048] Step a: Take a sample of 1 capsule, take out the contents of the capsule, grind it, add it to 3.0ml of water, shake it to dissolve, let it stand still, and prepare a sample solution;

[0049] Step b: Slowly add 0.01ml of phenolphthalein ethanol solution to the sample solution, oscillate and shake well, slowly drop into 0.03ml0.01% sodium hydroxide solution, oscillate and shake well to obtain a mixed solution, observe the color change of the mixed solution, Carry out the judgment for the first time, after dripping described 0.01% sodium hydroxide solution, described mixed solution turns red, after shaking shakes up, observes that the red color of described mixed solution disappears, and the color of described mixed solution turns into nothing. If it is judged that there may be ribavirin in the sample, proceed to the following steps. The reaction formula is:

[...

technical field

[0001] The invention relates to the detection field, in particular to a detection method for illegally adding ribavirin in health products. Background technique

[0002] The chemical name of ribavirin is 1-β-D-ribofuranose-1,2,4-triazole-3-hydroxyamide, which is a broad-spectrum and potent antiviral drug, mainly used to treat various viral infections Diseases are currently widely used in the prevention and treatment of common viral diseases. Common dosage forms include injections, tablets, oral liquids, aerosols, etc. The reported adverse reactions of ribavirin include allergic reactions, anaphylactic shock, blood system reactions, nervous system reactions, circulatory system reactions, damage to the skin and mucous membranes and their accessories, teratogenicity, and fetal death. Therefore, ribavirin should be taken under the guidance of a physician, and the indications and contraindications should be clarified and used reasonably.

[0003] However, in or...

Examples

Embodiment 1

[0031] The concentrated hydrochloric acid in this example is the analytically pure concentrated hydrochloric acid supplied by Nanjing Chemical Reagent Co., Ltd., the analytically pure acetic acid supplied by Xilong Science Co., Ltd. for acetic acid, and the analytically pure anhydrous sodium acetate supplied by Sinopharm Chemical Reagent Co., Ltd. For sodium acetate and phenolphthalein, select analytically pure phenolphthalein supplied by Xilong Science Co., Ltd., for ethanol, select analytically pure absolute ethanol supplied by Xilong Science Co., Ltd., and for sodium hydroxide, select analytically pure sodium hydroxide supplied by Sinopharm Chemical Reagent Co., Ltd. For chloroform, select analytically pure chloroform supplied by Nanjing Chemical Reagent Co., Ltd., for calcium oxide, select analytically pure calcium oxide supplied by Nanjing Chemical Reagent Co., Ltd., and for tert-butyldiphenylchlorosilane (TBDPSCL), select Alfa Aesar ( Analytical pure tert-butyldimethylchl...

Embodiment 2

[0042] A method for detecting illegally added ribavirin in health products, comprising the following steps:

[0043] Step a: Take a sample of 1 capsule, take out the contents of the capsule, grind it, add it to 3ml of water, shake it to dissolve, let it stand still, and prepare a sample solution;

[0044]Step b: Slowly add 0.01ml of phenolphthalein ethanol solution to the sample solution, oscillate and shake well, slowly drop into 0.03ml0.01% sodium hydroxide solution, oscillate and shake well to obtain a mixed solution, observe the color change of the mixed solution, Carry out the first judgment, after dripping the 0.01% sodium hydroxide solution, the mixed solution turns red, after shaking and shaking, it is observed that the red color of the mixed solution does not disappear, and it is judged that there is no ribavirin in the sample , terminate the detection;

[0045] The sample solution is colorless after adding phenolphthalein ethanol solution, indicating that the sample...

Embodiment 3

[0047] A method for detecting illegally added ribavirin in health products, comprising the following steps:

[0048] Step a: Take a sample of 1 capsule, take out the contents of the capsule, grind it, add it to 3.0ml of water, shake it to dissolve, let it stand still, and prepare a sample solution;

[0049] Step b: Slowly add 0.01ml of phenolphthalein ethanol solution to the sample solution, oscillate and shake well, slowly drop into 0.03ml0.01% sodium hydroxide solution, oscillate and shake well to obtain a mixed solution, observe the color change of the mixed solution, Carry out the judgment for the first time, after dripping described 0.01% sodium hydroxide solution, described mixed solution turns red, after shaking shakes up, observes that the red color of described mixed solution disappears, and the color of described mixed solution turns into nothing. If it is judged that there may be ribavirin in the sample, proceed to the following steps. The reaction formula is:

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