Method for detecting illegally added ribavirin in health care product

Abstract

The invention discloses a method for detecting illegally added ribavirin in a health care product. The method comprises the following steps: a phenolphthalein-ethanol solution, a sodium hydroxide solution, tert-butyl diphenyl chlorosilane, a pyridine aqueous solution, a catalyst DMAP aqueous solution, chloroform, a hydrochloric acid solution and excessive calcium oxide are added into a sample to be detected, wet litmus test paper is used, and whether the sample contains ribavirin is determined according to twice chromogenic reaction. All used reagents are common chemical reagents, the identification method is sensitive to reaction, high in specificity and obvious in phenomena, large instruments are not needed, operation is simple and safe, the cost is lot, and the method is suitable for large-batch onsite rapid detection. The detection limit of illegally added ribavirin in the health care product is 1.0 mg / time.

Description

technical field [0001] The invention relates to the detection field, in particular to a detection method for illegally adding ribavirin in health products. Background technique [0002] The chemical name of ribavirin is 1-β-D-ribofuranose-1,2,4-triazole-3-hydroxyamide, which is a broad-spectrum and potent antiviral drug, mainly used to treat various viral infections Diseases are currently widely used in the prevention and treatment of common viral diseases. Common dosage forms include injections, tablets, oral liquids, aerosols, etc. The reported adverse reactions of ribavirin include allergic reactions, anaphylactic shock, blood system reactions, nervous system reactions, circulatory system reactions, damage to the skin and mucous membranes and their accessories, teratogenicity, and fetal death. Therefore, ribavirin should be taken under the guidance of a physician, and the indications and contraindications should be clarified and used reasonably. [0003] However, in or...

AI Technical Summary

Problems solved by technology

It is characterized in that the disadvantages of thin-layer chromatography are that it has high requirements for spot plate technology, the edge effect is difficult to overcome, and there are many interference factors, which affect the accuracy of the test results.

The cost of chromogenic reaction analysis is low, but the specificity of this method is not strong, the sensitivity is poor, and the anti-interference ability is poor

The ammonia odor produced during the experiment is strong and not friendly to the human body and the environment, so the use is limited, and a boiling water bath is required for heating, and heating tools are required, which is not suitable for rapid on-site detection

Moreover, the heating operation will cause the solution to spray out, which is not safe enough.

Patent application number 201711078153.8, a patent document for ribavirin detection test strips, discloses the use of colloidal gold method to detect ribavirin, however, this detection method requires the use of exclusive colloidal gold cards, made of colloidal gold cards The process is difficult and difficult to replicate, has high requirements for technology and environment, and the cost is too high, so it is not suitable for mass testing

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