Method for analyzing lipid plaque content by fixing major arteries of rabbits

A lipid plaque and large artery technology, applied in the field of biological experiments, can solve the problems of unintuitive scanning images, time-wasting, insufficient scanning, etc., and achieve the effect of avoiding arterial curling and improving analysis efficiency

Inactive Publication Date: 2020-04-07
YANGZHOU UNIV
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Problems solved by technology

[0003] However, because there is no good method to fix the rabbit artery at present, the rabbit artery cannot be flattened and fixed for

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  • Method for analyzing lipid plaque content by fixing major arteries of rabbits

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Embodiment Construction

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0022] see figure 1 , example:

[0023] A method for fixing a rabbit aorta to analyze lipid plaque content, comprising the following steps:

[0024] A. Select raw materials: without perfusion, take out the complete branches of the neck and iliac part of the rabbit including the artery, and peel off the excess peripheral fat;

[0025] B. Artery section: stop along the upper edge of the aortic arch to the root of the aorta, cut the lower edge of the aorta to t...

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Abstract

The invention relates to the technical field of biological experiments, in particular to a method for analyzing lipid plaque content by fixing major arteries of rabbits. The method comprises the following steps: A, selection of raw materials; B, arterial choice; C, artery shaping: washing an artery sample clean, and soaking the artery sample in a pre-prepared paraformaldehyde stationary liquid for24 hours; D, fat marking: taking out the shaped artery sample, splitting the artery sample from top to bottom, and dyeing the lipid plaques by adopting a Sudan IV dyeing method; E, preparing of a sample plate: adhering a black double-sided adhesive tape to the transparent sample plate, and adhering the dyed artery sample to the surface layer of the black double-sided adhesive tape; and F, scanning analysis. According to the invention, a proper fixing mode is adopted, the artery can be quickly flattened, so that a clear artery inner wall lipid plaque map can be obtained, shaping of the arteries by adopting the paraformaldehyde stationary liquid are fat marking by a Sudan IV coloring agent are completed in order, the arteries can be prevented from curling or being unobviously distinguishedby using the sticking mode, and the arteries can be rapidly and comprehensively scanned by using the finally prepared sample plate, so that the rabbit artery fixing is simplified, and the lipid content analysis efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of biological experiments, in particular to a method for fixing the aorta of a rabbit to analyze the content of lipid plaque. Background technique [0002] Rabbit is a commonly used research object in biological experiments. For example, the research on the content of lipid plaque in rabbit aorta can help technicians better understand the physiological structure of similar organisms, which will help to carry out other related research in the later stage . [0003] However, due to the lack of a good method to fix the rabbit artery, the rabbit artery cannot be flattened and fixed for scanning, often resulting in insufficient scanning, time-consuming multiple scans, and unintuitive scanning images. Contents of the invention [0004] The purpose of the present invention is to provide a method for fixing the aorta of rabbits to analyze the content of lipid plaque, which has the advantages of convenient fixing ...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/305G01N2001/302
Inventor 梁景岩严坤宁王英歌庄文雯
Owner YANGZHOU UNIV
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