SNP molecular marker related to Cucumis sativus L multiple-trichome character, and application of SNP molecular marker

A technology of molecular markers and epidermis, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of molecular marker-assisted breeding and other problems, achieve convenient identification, solve low reliability, and gene loci point precise effect

Active Publication Date: 2021-02-05
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most cucumber trichome-deficient mutants have a hairless phenotype, but the research on cucumber hirsutism is still blank, which brings difficulties to the genetic improvement and molecular marker-assisted breeding of cucumber fruit skin-related traits

Method used

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  • SNP molecular marker related to Cucumis sativus L multiple-trichome character, and application of SNP molecular marker
  • SNP molecular marker related to Cucumis sativus L multiple-trichome character, and application of SNP molecular marker
  • SNP molecular marker related to Cucumis sativus L multiple-trichome character, and application of SNP molecular marker

Examples

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Embodiment 1

[0032] Example 1 Cucumber multiple epidermis trait linkage molecular markers are obtained by the following methods:

[0033] (1) Acquisition and genetic analysis of the trait of multiple epidermis in cucumber

[0034] Cucumber multiple trichomes mutant mt was obtained by mutagenizing the northern cucumber cultivar 'Changchun Mici' with EMS (ethyl methanesulfonate). Compared with wild-type plants, the mt mutant had more epidermal trichomes on the surface of leaves, stems, tendrils, floral organs and ovaries. F1 plants of WT and mt crosses exhibited an intermediate phenotype with a moderate number of epidermal trichomes on the surface. The length of the epidermal trichomes of the mt mutant was shorter than that of the wild type, and the length of the epidermal trichomes of the F1 was intermediate between the parents. Microscopic observation revealed that the number, size, arrangement and shape of epidermal trichomes in leaves of mt were different from those of wild type. In a...

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Abstract

The invention discloses an SNP molecular marker related to a Cucumis sativus L multiple-trichome character, and application of the SNP molecular marker. The SNP molecular marker is a primer designed by taking each 400bp of DNA sequence before and behind a Chr6:27397366 site in a "Chinese Long v2" Cucumis sativus L genome database as a template, a genome sequence obtained by amplification is disclosed by SEQ ID NO:1, and from a 5' terminal, A / C polymorphism, i.e., an SNP site, is in the presence on the 401st site of the sequence. The molecular marker disclosed by the invention is tightly linkedwith a Cucumis sativus L multiple-trichome gene, is reliable, is quick in detection, can be used for the directional genetic breeding of Cucumis sativus L multiple-trichome related characters, and has an important theoretical and practical guidance meaning for quickening the genetic improvement progress of a Cucumis sativus L variety and improving breeding selection efficiency.

Description

technical field [0001] The invention relates to a SNP molecular marker related to the multi-skinned trichomes trait of cucumber and an application thereof, belonging to the field of molecular genetic breeding of cucumber. Background technique [0002] Epidermal hairs (Trichomes) develop from epidermal cells. It is divided into unicellular or multicellular, some branched and some not branched. Some of the epidermis have glands, which can secrete some lipid substances or secondary metabolites, and some have no glands. Different types of epidermis can be produced on the same plant. Plant epidermal trichomes have a series of functions: non-glandular epidermal trichomes can dissipate heat, increase tolerance to chilling damage, facilitate seed dispersal, absorb water, protect from ultraviolet rays and damage plant tissues from some herbivorous insects; glandular trichomes Cuticle hairs help plants resist herbivorous insects and pathogens by secreting chemicals, but they also ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2600/172C12Q2531/113
Inventor 娄群峰杨志歌宋蒙飞陈劲枫
Owner NANJING AGRICULTURAL UNIVERSITY
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