A kind of composition and application and diagnosis kit
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A diagnostic kit and marker technology, applied in the field of analytical chemistry, to achieve the effects of good diagnostic accuracy, good diagnostic ability and high AUC
Active Publication Date: 2022-03-22
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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This study found that the concentrations of glucose-6-phosphate, erythrose-4-phosphate, xanthine, and phosphatidylcholine PC (33:1) in the benign pulmonary nodules and the lesion tissues of lung cancer patients were significantly different. None of the four metabolites combined was reported for the diagnosis of lung cancer and benign pulmonary nodules
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Embodiment 1
[0024] 1. Collection of Tissue Samples
[0025] Before sampling, all volunteers included in the study signed the informed consent.
[0026] Under the same conditions, 85 cases of lung cancer patients were collected from tumor lesion area tissues, including 18 cases of benign pulmonary nodules and 47 cases of lung cancer. Of the 47 cases of lung cancer, 17 cases belonged to early-stage lung cancer patients. All patients should have an enema or laxative one day before lung tumor resection, fast for more than 8 hours before surgery, and smokers should quit smoking for at least 2-3 weeks before surgery. The tissue samples collected separately were first dipped in blood on the surface through gauze, then quickly transferred to liquid nitrogen for short-term storage, and finally transferred to -80°C refrigerator for long-term storage.
[0027] 2. Analysis method
[0028] 2.1 Tissue samples were pretreated separately
[0029] Tissue samples were thawed on ice at room temperature, ...
Embodiment 2
[0053] In order to verify that the combined markers also have a good diagnostic effect on patients with early-stage lung cancer, 20 samples of early-stage lung cancer were added for verification. Analytical method, instrument and reagent used in this experiment are all completely consistent with embodiment 1.
[0054] 1. Before the collection of tissue samples, the included volunteers signed the informed consent.
[0055]Under the same conditions, 20 cases of early lung cancer tissue samples were collected according to the method described in Example 1, preserved, and prepared for inspection.
[0056] 2. Tissue test and auxiliary diagnosis results
[0057] Compared with lung benign nodules, the concentrations of glucose-6-phosphate and erythrose-4-phosphate were significantly decreased in early lung cancer, and the concentrations of xanthine and phosphatidylcholine PC (33:1) were significantly increased in early lung cancer (See figure 2 ). At the same time, the relative ...
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Abstract
The present invention relates to the preparation of small molecule metabolites glucose-6-phosphate, erythrose-4-phosphate, xanthine and phosphatidylcholine PC (33:1) in tissue samples as combined metabolic markers for diagnosing subjects A new application of the kit in patients with lung cancer and benign pulmonary nodules. The present invention also relates to a kit for detecting patients with lung cancer and benign pulmonary nodules in a subject, by detecting the relative concentrations of the above-mentioned combined markers in tissue samples from the subject, and calculating the combined markers based on a binary logistic regression equation The P value of the marker variable is then based on the determined cutoff value (cutoff) to diagnose patients with lung cancer and benign pulmonary nodules. In addition, the combined marker also has good diagnostic ability for patients with early lung cancer and benign pulmonary nodules. The kit can realize highly sensitive and efficient detection of several small molecule metabolites involved in the present invention, and has the characteristics of low detection cost and good repeatability.
Description
technical field [0001] The present invention relates to the use of a new combined marker in the preparation of kits for diagnosing lung cancer and lung cancer benign nodules. It belongs to the field of analytical chemistry, biochemistry and clinical medicine. Background technique [0002] Lung cancer is the malignant tumor with the highest mortality rate in the world. The lack of obvious symptoms of early lung cancer and the interference of other benign pulmonary nodules are difficult problems in the clinical diagnosis of lung cancer. Although the study of lung cancer has always been a research hotspot in oncology and clinical medicine, its pathogenic mechanism has not yet been clarified, which involves the combined effects of polygenic inheritance, environmental factors and lifestyle. Currently, serum tumor markers and imaging examinations are the main methods for lung cancer screening. However, the serum tumor marker carcinoembryonic antigen has low specificity and sens...
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/57484G01N33/57423G01N2800/7066
Inventor 许国旺由蕾刘心昱
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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