A method of using high temperature to cultivate gynogenetic bream pseudo-male fish

A technology of gynogenesis and bream, applied in the biological field, can solve problems such as water environmental pollution and hidden safety hazards of aquatic products

Active Publication Date: 2022-05-17
HUNAN NORMAL UNIVERSITY
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  • Description
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Problems solved by technology

However, hormone treatment faces problems such as water pollution and aquatic product safety hazards. It is necessary to further seek safe, efficient and environmentally friendly fish sex reversal technology

Method used

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  • A method of using high temperature to cultivate gynogenetic bream pseudo-male fish
  • A method of using high temperature to cultivate gynogenetic bream pseudo-male fish
  • A method of using high temperature to cultivate gynogenetic bream pseudo-male fish

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Embodiment

[0025] A method for cultivating gynogenetic bream pseudo-male fish at high temperature, comprising the steps of:

[0026] (1) Sperm genetic inactivation: take the male crucian carp (1-2 ages) obtained after artificial induction, extrude the semen and dilute it with Hank's solution at a volume ratio of 1:200, spread it on a petri dish, and irradiate it with a 15W ultraviolet lamp for 2 ~5min to complete the genetic inactivation of crucian carp sperm;

[0027] (2) Artificial gynogenetic development of bream: 2-3-year-old common bream is used as the parent fish to artificially induce labor, and genetically inactivated sperm are used to artificially inseminate the eggs of bream, and the obtained "fertilized eggs" Place it in a petri dish for 3 minutes in static water at 22°C-25°C, then transfer the fertilized eggs to water at 0°C-4°C for cold shock treatment for 22-26 minutes, and then place them in water at 22°C-25°C for static water incubation to obtain artificial females. nucl...

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Abstract

The invention discloses a method for cultivating pseudo-male fish of gynogenetic bream at high temperature, comprising the following steps: cultivating artificial gynogenetic bream all-female population fry at 24±1°C for 15-20 days, After being transferred to a water body at 34±1°C for 3-4 months of continuous high-temperature induction, it was transferred to a pond for routine feeding, and the gynogenetic bream pseudo-male was obtained. The method of the present invention does not use exogenous hormones, and uses the method of temperature-controlled cultivation to successfully realize the sex reversal of the gynogenetic bream, and the survival rate of the gynogenetic bream during the high-temperature treatment process of the present invention is 70-80% , the sex reversal rate is 10‑20%, which is of great significance for the propagation of artificial gynogenetic bream and the cultivation of new breeds of bream.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for cultivating gynogenetic giant bream pseudo-male fish by using high temperature. Background technique [0002] Artificial gynogenesis is an effective way for fish genetic improvement, and it plays an important role in the purification and rejuvenation of fish germplasm and the selection of improved species. Bram bream is an important freshwater cultured fish in my country, but the germplasm of bream is degraded at present, which seriously restricts the promotion and development of its culture. The artificial gynogenetic bream produced by gynogenetic technology is an all-female population, and new breeding strains with good quality can be obtained through inbreeding, which has important economic value and social effects in production and application. However, artificial gynogenesis includes double operations of sperm genetic inactivation and chromosome...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 肖亚梅符文彭亮跃刘文彬刘锦辉
Owner HUNAN NORMAL UNIVERSITY
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