Supercharge Your Innovation With Domain-Expert AI Agents!

Splitting, binding, washing and/or eluting reagent for separating and/or purifying RNA

A reagent and biological technology, applied in the field of extraction kits, can solve the problems of time-consuming and inability to extract polyphenol polysaccharide plant RNA, etc., and achieve the effect of a wide range of applications

Pending Publication Date: 2021-10-01
汉远化生医国际科技(北京)有限公司
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] First, the Trizol method and the guanidine isothiocyanate method cannot be used to extract polyphenol polysaccharide plant RNA; secondly, the above-mentioned t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Splitting, binding, washing and/or eluting reagent for separating and/or purifying RNA
  • Splitting, binding, washing and/or eluting reagent for separating and/or purifying RNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Example 1 - Extraction of RNA from room temperature pothos leaves

[0101] As mentioned above, take about 100mg of pothos radix leaves, treat with liquid nitrogen freezing and grinding method, transfer to 600ul lysate (3.5M NaCl, 3% glycerol, 100mM PH4.5NaAc, 20ul 2-mercaptoethanol) and shake well, add 300ul redistilled phenol, shake well, centrifuge at 12000rpm for 1 minute, take the supernatant, add 0.6 times the volume of supernatant absolute ethanol, shake well and transfer to the spin column, centrifuge at 12000rpm for 30 seconds, wash with 500ul 75% ethanol for 2 Once, the RNA was eluted from the spin column with 100ul eluent.

[0102] The results were determined on biaosharp (Table 1), and detected by gel electrophoresis ( figure 1 ), it can be seen that the method of the present invention extracts the RNA of the leaves of the pothos at room temperature.

[0103] Table 1: Quality of RNA extracted from pothos radish leaves at room temperature

[0104] ...

Embodiment 2

[0105] Example 2——Extraction of mouse pancreas tissue RNA at room temperature

[0106] According to the above, about 30mg of mouse pancreas tissue was taken, treated with liquid nitrogen cryogrinding method, and transferred to 500ul lysis solution (2% NP40, 1.8M NaCl, 200mM NaAc pH4.5, 3% glycerol, 20ul 2 -Mercaptoethanol) and shake well, add 100ul 5M NaCl, shake well, then add 400ul redistilled phenol, shake well, centrifuge at 12000rpm for 1 minute, take the supernatant, add 0.8 times the volume of supernatant absolute ethanol, shake well and transfer to The spin column was centrifuged at 12000 rpm for 30 seconds, washed twice with 500ul 75% ethanol, and the RNA was eluted from the spin column with 100ul eluent.

[0107] The results were determined on biaosharp (Table 2), and detected by gel electrophoresis ( figure 2 ), it can be seen that the 2% NP40RNA kit of the present invention can extract mouse pancreas tissue RNA.

[0108] Table 2: Quality of RNA extracted from pa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a splitting, binding, washing and/or eluting reagent for separating and/or purifying RNA as well as a method and a kit for separating and/or purifying RNA. The method has the advantages that 1, the operation is simple; 2, less time is consumed; 3, RNA is extracted at room temperature; and 4, the application range is wide, including polyphenol polysaccharide plant RNA extraction.

Description

【Technical field】 [0001] The present invention relates to lysing, binding, washing and / or eluting reagents, methods for isolating and / or purifying RNA and extraction kits for isolating and / or purifying RNA. 【Background technique】 [0002] In order to better study and understand the biological functions of RNA, some methods for extracting RNA from biological tissues have been developed. Traditional biological RNA extraction methods mainly include Trizol method, SDS method, CTAB method, guanidine isothiocyanate method, etc. [0003] Firstly, the Trizol method and the guanidine isothiocyanate method cannot be used to extract polyphenol polysaccharide plant RNA; secondly, the above-mentioned traditional RNA extraction methods cannot extract biological RNA at room temperature; thirdly, the above-mentioned traditional RNA extraction methods are time-consuming. [0004] In order to overcome the shortcoming of traditional biological RNA extraction method, the present invention prov...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/10
CPCC12N15/1003C12N15/1006
Inventor 张怀远安豹王治元
Owner 汉远化生医国际科技(北京)有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com