Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for distinguishing authenticity of selaginella tamariscina varieties in non-targeted metabonomics and application

A metabolomics, non-targeted technology, applied in the field of Selaginella identification, can solve the problems of inaccurate quantitative results, inability to effectively extract Selaginella-specific markers, and cumbersome extraction methods.

Pending Publication Date: 2022-07-29
湖南省药品检验检测研究院 +1
View PDF22 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technique uses advanced techniques from large sample collection (metabotrophy) research that allows us to identify different types of compounds called sialic acid or glucose attached to certain molecules such as lipids found within cells during cell division. By analyzing these substances with various methods like mass spectrometry, gas chromatography, liquid chromato graphy, X ray diffraction, nuclear magnetic resonance spectroscopy, etc., we are able to accurately determine how much each type of sugar was added by studying changes caused by environmental factors affects gene expression patterns. These results help scientists better study biological systems more effectively.

Problems solved by technology

This patented technical problem addressed in this patent relates to identifying certain substances called seeds associated with various plants like Celasma Cordgrass Grunting Flower Leech Silver Needles, Belvidium Cortex Microscope Grape seed Extract, and Essential Oil Manufacture Technologies. Existing techniques involve complicated procedures involving multiple stages including centrifugal sorting followed by purification processes. There has long existed difficulties in achieving reliable recognition between these sources due to their complex nature.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for distinguishing authenticity of selaginella tamariscina varieties in non-targeted metabonomics and application
  • Method for distinguishing authenticity of selaginella tamariscina varieties in non-targeted metabonomics and application
  • Method for distinguishing authenticity of selaginella tamariscina varieties in non-targeted metabonomics and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] The preparation procedure of the samples of the present invention investigated different extraction solvents (50% methanol, 70% methanol and 100% methanol), different extraction methods (ultrasonic and reflux extraction) and extraction time (30 minutes, 45 minutes, 60 minutes and 75 minutes) .

[0042] like Image 6 As shown, 70% methanol solution can extract Selaginellin and Selaginellin A specific markers, but 50% methanol and 100% methanol cannot extract Selaginella specific markers.

[0043] like Figure 7 As shown, Selaginellin and Selaginellin A can be extracted with reflux extraction time of 60 minutes, but the amount of Selaginellin and Selaginellin A extracted by ultrasonic extraction for 60 minutes is very small, and no obvious peak can be seen from the figure.

[0044] like Figure 8 As shown, when the reflux extraction time was 50-75 min, the content of specific markers of Selaginella serrata extracted was higher.

[0045] Therefore, it was finally deter...

Embodiment 1

[0047] The present invention will be further described below with reference to the accompanying drawings.

[0048] 1. Preparation of Selaginella and Selaginella contamination samples:

[0049] Pulverize Selaginella and Selaginella mixed product, add 30 mL of 70% methanol solution per 0.27-0.33 g of sample powder, seal it, weigh it, extract under reflux, cool, make up the weight with methanol, filter, and take the subsequent filtrate, 18000 g × Centrifuge at 4 degrees and get the supernatant.

[0050] 2. LC-MS detection of Selaginella and Selaginella contamination sample components:

[0051] Chromatographic conditions: chromatographic column ACQUITY UPLC RBEH C18 column (100mm×2.1mm, 1.7μm); mobile phase is 0.1% formic acid aqueous solution-acetonitrile, gradient elution program is 0~10min, 5~15% acetonitrile; 10~20min, 15%~35% acetonitrile; 20~30min, 35%~55% acetonitrile; 30~40min, 55%~85% acetonitrile; 40~45min, 85%~95% acetonitrile; 45~50min, 95%~95% % acetonitrile; flow ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Volume concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a non-targeted metabonomics selaginella tamariscina variety authenticity distinguishing method and application, and the method comprises the following steps: S1, respectively crushing selaginella tamariscina and selaginella tamariscina adulterants, respectively adding a methanol solution with the volume concentration of 55-85% v/v for reflux extraction, filtering, and taking filtrate to respectively prepare a selaginella tamariscina extracting solution and a selaginella tamariscina adulterant extracting solution; s2, detecting and analyzing the selaginella tamariscina extracting solution; s3, obtaining an identification model to distinguish selaginella tamariscina and selaginella tamariscina And S4, determining the content of the differential marker determined in S3 based on an ultra-high performance liquid chromatography-mass spectrometer, and establishing the quality standard of selaginella tamariscina. The difference between the selaginella tamariscina and the selaginella tamariscina adulterants is clarified, it is indicated that metabonomics can be used for distinguishing the selaginella tamariscina and the selaginella tamariscina adulterants, the method is advanced, the experimental result is reliable, and an excellent method is provided for authentic selaginella tamariscina variety identification and quality evaluation.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Owner 湖南省药品检验检测研究院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com