The invention discloses a preparation method and application of a
selaginella tamariscina extract. The method comprises the following steps that 1, a
solvent is added into smashed
selaginella tamariscina, extraction is conducted at the temperature of 0-80 DEG C,
herb residues are removed through
centrifugation, and a centrifugate is obtained; the centrifugate is concentrated, the pH value of the centrifugate is adjusted to be alkaline, standing and
centrifugation are conducted, and
sediment is collected and obtained; 2, after the
sediment is dissolved by adding water, the pH value is adjustedto be acidic, decoloration is conducted to obtain the
selaginella tamariscina extract. The method is simple in operation, the extracting efficiency is high, the content of total
polyphenol in the obtained
selaginella tamariscina extract is high, and at the
relative density of 1.010-1.090, the content of the total
polyphenol in the obtained
selaginella tamariscina extract is higher than or equal to50 mg / mL. The
selaginella tamariscina extract is high in safety performance and has the advantages that free radicals are remarkably removed, oxidation is resistant, and proliferation of human embryonic
kidney cells is promoted; the extract is a
liquid product, so that the extract is conveniently added into
cosmetics and suitable for industrialized application and popularization.