Hair conditioner containing beta-D-glucan of Candida utilis
A technology for producing Candida utilis and glucan, which is applied in the field of daily chemicals, can solve the problems of unsatisfactory moisturizing effect and anti-oxidation effect, side effects, etc., and achieves a simple and easy operation process, reduces pollution, and avoids degradation. Effect
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Embodiment 1
[0039] Candida utilis β-D-glucan prepared by a method comprising the following steps:
[0040] 1) Cultivate Candida utilis in protein-free potato juice for 14 hours, then centrifuge, and rinse with deionized water to obtain Candida utilis cells; the used Candida utilis (Candidautilis), preservation number CICC1769 ;
[0041] 2) The obtained Candida utilis cells were added to a sodium chloride solution with a mass percentage concentration of 5%, and were shaken and autolyzed at pH 7.0, 55-60° C., and 120 rpm for 24 hours. After autolysis, the centrifugal force was 3000g, centrifuge at 4°C and wash with deionized water;
[0042] 3) Add the obtained Candida utilis cells into the phosphate buffer of pH 6.0, in the phosphate buffer, the mass percentage concentration of the yeast cells is 10%, suspend the Candida utilis cells, and then add the diameter 0.5cm glass beads, solid-liquid ratio 1:15, high-temperature leaching, leaching time 2h, temperature 120°C, centrifugation, washin...
Embodiment 2
[0049] Candida utilis β-D-glucan prepared by a method comprising the following steps:
[0050]1) Cultivate Candida utilis in protein-free potato juice for 14 hours, then centrifuge, and rinse with deionized water to obtain Candida utilis cells; the used Candida utilis (Candidautilis), preservation number CICC1769 ;
[0051] 2) The obtained Candida utilis cells were added to a sodium chloride solution with a mass percentage concentration of 5%, and were shaken and autolyzed at pH 7.0, 55-60° C., and 120 rpm for 24 hours. After autolysis, the centrifugal force was 3000g, centrifuge at 4°C and wash with deionized water;
[0052] 3) Add the obtained Candida utilis cells into the phosphate buffer of pH 6.0, in the phosphate buffer, the mass percentage concentration of the yeast cells is 10%, suspend the Candida utilis cells, and then add the diameter 0.5cm glass beads, solid-liquid ratio 1:15, high-temperature leaching, leaching time 2h, temperature 120°C, centrifugation, washing...
Embodiment 3
[0058] Candida utilis β-D-glucan prepared by a method comprising the following steps:
[0059] 1) Cultivate Candida utilis in protein-free potato juice for 14 hours, then centrifuge, and rinse with deionized water to obtain Candida utilis cells; the used Candida utilis (Candidautilis), preservation number CICC1769 ;
[0060] 2) The obtained Candida utilis cells were added to a sodium chloride solution with a mass percentage concentration of 5%, and were shaken and autolyzed at pH 7.0, 55-60° C., and 120 rpm for 24 hours. After autolysis, the centrifugal force was 3000g, centrifuge at 4°C and wash with deionized water;
[0061] 3) Add the obtained Candida utilis cells into the phosphate buffer of pH 6.0, in the phosphate buffer, the mass percentage concentration of the yeast cells is 10%, suspend the Candida utilis cells, and then add the diameter 0.5cm glass beads, solid-liquid ratio 1:15, high-temperature leaching, leaching time 2h, temperature 120°C, centrifugation, washin...
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