Detection method of myrtle fruit medicinal material

Abstract

The invention relates to the technical field of traditional Chinese medicine, and is a method for detecting a myrtle fruit medicinal material. The method for detecting the myrtle fruit medicinal material comprises the following steps: first, thin layer chromatography identification of gallic acid and ellagic acid in myrtle fruit; second, thin layer chromatography identification of quercetin and hyperoside in myrtle fruit; third, determination of extracts in myrtle fruit; and fourth, determination of the content of quercetin in myrtle fruit medicinal material. The method is simple to operate, rapid, stable, reliable, accurate, and highly specific, and is effectively used for detecting myrtle fruit medicinal materials, provides technical guarantee for further development of identification of myrtle fruit medicinal materials and control of intrinsic quality, and lays a foundation for further in-depth research on myrtle fruit.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines and relates to a method for detecting myrtle fruit medicinal materials. Background Art

[0002] Myrtle fruit, also known as myrtle fruit, also known as Aibrias, is a plant of the Myrtaceae family Myrtle Myrtus communis L. Nearly ripe fruit. There is a small amount of cultivation in some parts of my country, and it is mainly produced in India, Sri Lanka and Europe. It has the effects of cooling blood and stopping bleeding, drying dampness and stopping diarrhea, astringing and stopping sweating, anti-inflammatory and analgesic, nourishing hair roots, etc. It is mainly used to treat damp-heat or blood-related diseases, such as hot bleeding, gingival bleeding, hematuria and astringent urine, menorrhagia, wet diarrhea, sweating, various inflammations, abscesses, hair loss, etc. There are few documents on the fruit of the myrtle, and only volatile oils, flavonoids, sugars and organic acids can be found, but there are no specific indicator components. The current legal standard for the fruit of the myrtle is included in the "Drug Standards of the Ministry of Health of the People's Republic of China", but the content of the standard is relatively simple, with only properties and microscopic identification items, and its quality cannot be well controlled. Summary of the invention

[0003] The invention provides a method for detecting a myrtle fruit medicinal material, which overcomes the deficiencies of the prior art and can effectively solve the problems of unclear chemical components of the myrtle fruit and lack of effective and feasible qualitative and quantitative methods in the prior art.

[0004] The technical scheme of the present invention is realized by the following measures: a detection method for a myrtle fruit medicinal material, which is carried out according to the following steps: the first step is to identify gallic acid and ellagic acid in myrtle fruit by thin layer chromatography; the second step is to identify quercetin and hyperoside in myrtle fruit by thin layer chromatography; the third step is to determine the extract in myrtle fruit; and the fourth step is to determine the content of quercetin in the myrtle fruit medicinal material.

[0005] The following are further optimizations and / or improvements to the above technical solutions:

[0006] In the first step above, the step of thin layer chromatography identification of gallic acid and ellagic acid in myrtle fruit is: prepare a first test solution, prepare ellagic acid reference solution and gallic acid reference solution, respectively absorb the ellagic acid reference solution and the gallic acid reference solution, the first test solution, spot on the same silica gel thin layer plate, use toluene-ethyl formate-formic acid with a volume ratio of 5:5:0.4 as a developing agent, develop, dry, spray with an equal amount of a mixed solution of 2% ferric chloride solution and 2% potassium ferrocyanide solution, and inspect under sunlight. In the thin layer chromatogram of the test sample, spots of the same color appear at the corresponding position of the thin layer chromatogram of the reference sample.

[0007] In the above second step, the steps of thin layer chromatography identification of quercetin and hyperoside in myrtle fruit are: preparing a second test solution, preparing a quercetin reference solution and a hyperoside reference solution, respectively aspirating the quercetin reference solution, the hyperoside reference solution, and the second test solution, spotting them on the same silica gel thin layer plate, using ethyl acetate-formic acid-water with a volume ratio of 8:1:1 as a developing agent, developing, taking out, drying, spraying with 5% aluminum chloride ethanol solution, heating at 105°C for 2 minutes, and examining under ultraviolet light. In the thin layer chromatogram of the test sample, fluorescent spots of the same color appear at the corresponding positions of the thin layer chromatogram of the reference sample.

[0008] In the third step, water is used as the extraction solvent for the determination of the extract in the myrtle fruit, and the hot soaking method is used as the determination method for the extract in the myrtle fruit.

[0009] In the fourth step, the content of quercetin in the myrtle fruit wood medicinal material is determined by liquid chromatography.

[0010] In the first step above, the preparation method of the first test solution is: take 1g to 5g of myrtle fruit powder, add 20mL to 30mL of 40% ethanol, heat and reflux for 30 minutes, filter, evaporate the filtrate to dryness, add 20mL of water to dissolve the residue after the filtrate is evaporated to dryness, extract twice with 15mL to 30mL of ethyl acetate after the residue is dissolved, combine the extracts to obtain ethyl acetate solution, evaporate to dryness, and dissolve the evaporated ethyl acetate solution with 1mL of ethyl acetate to obtain the first test solution.

[0011] The preparation method of the above-mentioned second test solution is as follows: take 1g to 5g of myrtle fruit powder, add 15mL to 30mL of water, ultrasonically extract for 30 minutes, filter, discard the filtrate, add 40mL to 70mL of methanol to the residue, ultrasonically extract for 40 minutes, filter to obtain the methanol extract, evaporate the methanol extract to dryness, and then add 2mL of methanol to dissolve to obtain the second test solution.

[0012] In the fourth step, the liquid chromatography conditions are as follows: the chromatographic column is a C18 chromatographic column, the chromatographic column filler particle size is 5 μm, the mobile phase is a mixture of acetonitrile and 0.1% formic acid in a volume ratio of 20:80, the detection wavelength is 256 nm, the column temperature is 30° C., and the injection volume is 10 μL.

[0013] The method of the invention is simple to operate, rapid, stable, reliable, accurate, and highly specific, and can be effectively used for the detection of myrtle fruit medicinal materials, providing technical guarantee for the further development of myrtle fruit medicinal material identification and intrinsic quality control, and laying a foundation for further in-depth research on myrtle fruit. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Attached Figure 1This is a thin layer identification diagram of gallic acid and ellagic acid in Example 9 of the present invention, wherein serial number 8 is a gallic acid reference solution, serial number 9 is an ellagic acid reference solution, serial numbers 1 to 7 correspond to myrtle fruit samples No. 1 to 7, and serial numbers 10 to 17 correspond to myrtle fruit samples No. 8 to 15.

[0015] Attached Figure 2 This is a thin layer identification diagram of quercetin and hyperoside in Example 9 of the present invention, wherein serial number 8 is a quercetin reference solution, serial number 9 is a hyperoside reference solution, serial numbers 1 to 7 correspond to myrtle fruit samples No. 1 to 7, and serial numbers 10 to 17 correspond to myrtle fruit samples No. 8 to 15.

[0016] Attached Figure 3 The chromatogram is of the quercetin reference substance solution in Example 9 of the present invention.

[0017] Attached Figure 4 The chromatogram is of the myrtle fruit test solution in Example 9 of the present invention. DETAILED DESCRIPTION

[0018] The present invention is not limited by the following embodiments, and the specific implementation method can be determined according to the technical scheme of the present invention and the actual situation. Unless otherwise specified, the various chemical reagents and chemicals mentioned in the present invention are all chemical reagents and chemicals known and used in the prior art; unless otherwise specified, the percentages in the present invention are all mass percentages; unless otherwise specified, the solutions in the present invention are all aqueous solutions with water as the solvent, for example, hydrochloric acid solution is a hydrochloric acid aqueous solution; the normal temperature and room temperature in the present invention generally refer to a temperature of 15°C to 25°C, and are generally defined as 25°C.

[0019] There are a total of 15 batches of fragrant myrtle fruit samples used in the embodiments of the present invention (the corresponding batch numbers of the samples of sequence numbers 1 to 15 are 20160531, 20160605, 20160621, 20170506, 20160305, 20160815, Z30019901, Z30019932, Z30019809, Z30101501, Z30101523, Z30101615, Z30019903, Z30020007, and Z30020018, respectively), all of which are imported from Pakistan and are identified as the fragrant myrtle fruit studied in the present invention.

[0020] The present invention will be further described below in conjunction with embodiments:

[0021] Embodiment 1: The detection method of the myrtle fruit medicinal material is carried out according to the following steps: the first step is to identify gallic acid and ellagic acid in myrtle fruit by thin layer chromatography; the second step is to identify quercetin and hyperoside in myrtle fruit by thin layer chromatography; the third step is to determine the extract in myrtle fruit; the fourth step is to determine the content of quercetin in the myrtle fruit medicinal material.

[0022] Example 2: As an optimization of the above example, in the first step, the step of thin layer chromatography identification of gallic acid and ellagic acid in myrtle fruit is as follows: prepare a first test solution, prepare ellagic acid reference solution and gallic acid reference solution, respectively absorb the ellagic acid reference solution and the gallic acid reference solution, the first test solution, spot on the same silica gel thin layer plate, use toluene-ethyl formate-formic acid with a volume ratio of 5:5:0.4 as a developing agent, develop, dry, spray with an equal amount of a mixed solution of 2% ferric chloride solution and 2% potassium ferrocyanide solution, and inspect under sunlight. In the thin layer chromatogram of the test sample, spots of the same color appear at the corresponding position of the thin layer chromatogram of the reference sample.

[0023] Example 3: As an optimization of the above example, in the second step, the steps of thin layer chromatography identification of quercetin and hyperoside in myrtle fruit are as follows: prepare a second test solution, prepare a quercetin reference solution and a hyperoside reference solution, respectively absorb the quercetin reference solution, the hyperoside reference solution, and the second test solution, spot them on the same silica gel thin layer plate, use ethyl acetate-formic acid-water with a volume ratio of 8:1:1 as the developing agent, develop, take out, dry, spray with 5% aluminum chloride ethanol solution, heat at 105°C for 2 minutes, and examine under ultraviolet light. In the test thin layer chromatogram, fluorescent spots of the same color appear at the corresponding positions of the reference thin layer chromatogram.

[0024] Example 4: As an optimization of the above example, in the third step, water is used as the extraction solvent for determining the extract in the myrtle fruit, and the hot soaking method is used as the method for determining the extract in the myrtle fruit.

[0025] Example 5: As an optimization of the above example, in the fourth step, the content of quercetin in the medicinal material of Myrtles sinensis is determined by liquid chromatography.

[0026] Example 6: As an optimization of the above example, in the first step, the preparation method of the first test solution is: take 1g to 5g of myrtle fruit powder, add 20mL to 30mL of 40% ethanol, heat and reflux for 30 minutes, filter, evaporate the filtrate to dryness, add 20mL of water to dissolve the residue after the filtrate is evaporated, and after the residue is dissolved, extract it twice with 15mL to 30mL of ethyl acetate, combine the extracts to obtain ethyl acetate solution, evaporate to dryness, and dissolve the evaporated ethyl acetate solution with 1mL of ethyl acetate to obtain the first test solution.

[0027] Example 7: As an optimization of the above example, the preparation method of the second test solution is as follows: take 1g to 5g of myrtle fruit powder, add 15mL to 30mL of water, ultrasonically extract for 30 minutes, filter, discard the filtrate, add 40mL to 70mL of methanol to the residue, ultrasonically extract for 40 minutes, filter to obtain the methanol extract, evaporate the methanol extract to dryness, and then add 2mL of methanol to dissolve to obtain the second test solution.

[0028] Example 8: As an optimization of the above example, in the fourth step, the liquid chromatography conditions are: the chromatographic column is a C18 chromatographic column, the chromatographic column filler particle size is 5 μm, the mobile phase is a mixture of acetonitrile and 0.1% formic acid with a volume ratio of 20:80, the detection wavelength is 256 nm, the column temperature is 30°C, and the injection volume is 10 μL.

[0029] Example 9: The first step is to identify gallic acid and ellagic acid in myrtle fruit by thin layer chromatography.

[0030] Take 2 g of myrtle fruit powder, add 20 mL of 40% ethanol, heat and reflux for 30 minutes, filter, evaporate the filtrate to dryness, add 20 mL of water to the residue after the filtrate is evaporated to dryness to dissolve it, extract it twice with 20 mL of ethyl acetate after the residue is dissolved, combine the extracts to obtain ethyl acetate solution, evaporate to dryness, dissolve the evaporated ethyl acetate solution with 1 mL of ethyl acetate to obtain the first test solution.

[0031] Use methanol as solvent to prepare 1 mg / mL ellagic acid reference solution and gallic acid reference solution. Take 20 μL of ellagic acid reference solution, 5 μL of gallic acid reference solution, and 10 μL of the first test solution, respectively, and spot them on the same silica gel G thin layer plate. Use toluene-ethyl formate-formic acid with a volume ratio of 5:5:0.4 as the developing solvent. Develop, dry, spray with a mixed solution of 2% ferric chloride solution and 2% potassium ferrocyanide solution in equal amounts, and inspect under sunlight. In the thin layer chromatogram of the test sample, spots of the same color appear at the corresponding positions of the thin layer chromatogram of the reference sample. See the thin layer identification diagram of gallic acid and ellagic acid in the myrtle fruit sample. Figure 1 .

[0032] The second step is to identify quercetin and hyperoside in myrtle fruit by thin layer chromatography.

[0033] Take 2 g of myrtle fruit powder, add 20 mL of water, ultrasonically extract for 30 minutes, filter, discard the filtrate, add 50 mL of methanol to the residue and ultrasonically extract for 40 minutes, filter to obtain the methanol extract, evaporate the methanol extract to dryness and then add 2 mL of methanol to dissolve to obtain the second test solution.

[0034] Use methanol as solvent to prepare 0.5 mg / mL quercetin reference solution and hyperoside reference solution, respectively, take 5 μL of quercetin reference solution and hyperoside reference solution, and 10 μL of the second test solution, spot on the same silica gel G thin layer plate, use ethyl acetate-formic acid-water with a volume ratio of 8:1:1 as the developing solvent, develop, take out, dry, spray with 5% aluminum chloride ethanol solution, heat at 105°C for 2 minutes, and inspect under ultraviolet light at 365nm. In the thin layer chromatogram of the test sample, fluorescent spots of the same color appear at the corresponding positions of the thin layer chromatogram of the reference sample. See the thin layer identification diagram of quercetin and hyperoside in the myrtle fruit sample. Figure 2 .

[0035] The third step is to determine the extract of the myrtle fruit medicinal material.

[0036] Fifteen batches of myrtle fruit sample powder (passed through No. 2 sieve) were taken, water was used as the extraction solvent, and the hot soaking method was used as the myrtle fruit extract determination method. The specific data are shown in Table 3. The determination results were in the range of 43.8% to 57.2%. Based on the sample data, the extract limit was proposed to be 40%.

[0037] The fourth step is the liquid chromatography determination of the quercetin content in the myrtle fruit wood medicinal material.

[0038] Take an appropriate amount of quercetin reference substance, accurately weigh it, and add methanol to prepare a quercetin reference substance solution containing 50 μg of quercetin per 1 mL.

[0039] Take about 4.0 g of myrtle powder (passed through No. 3 sieve), accurately weigh it, place it in a stoppered conical flask, accurately add 20 mL of 80% methanol, stopper it, weigh it, heat it under reflux for 1 hour, cool it, weigh it again, make up the lost weight with 80% methanol, shake it well, filter it, and take the filtrate to obtain the myrtle test solution.

[0040] The quercetin reference solution and the myrtle fruit test solution were tested under the following chromatographic conditions to prepare a standard curve and verify the accuracy and reproducibility of the method.

[0041] Chromatographic column: Agilent 5 TC-C18(2) 4.6×250mm 5μm; mobile phase: acetonitrile-0.1% formic acid (20:80); detection wavelength: 256nm; column temperature: 30℃. Accurately pipette 10μL of reference solution and test solution respectively, inject into liquid chromatograph for determination.

[0042] Figure 3 , Figure 4 The chromatograms are of quercetin reference solution and myrtle fruit test solution. Figure 3 , Figure 4It can be seen that the characteristic absorption peak of quercetin appeared in both the quercetin reference substance and the myrtle fruit test substance at around 17.3 minutes, proving that this method has good specificity.

[0043] Embodiment 10: Selection of the determination method of the extract of the myrtle fruit medicinal material:

[0044] According to the provisions of "Determination of Extracts" under "General Rules for the Verification of Medicinal Materials and Decoction Pieces 0212" in Part IV of the 2020 edition of the Chinese Pharmacopoeia. In this example, water and ethanol solutions of different concentrations were selected, and the water-soluble and alcohol-soluble extracts of myrtle fruit were investigated by cold immersion and hot immersion, respectively, and the solvent was selected, and the myrtle fruit sample was measured.

[0045] Table 1 is a comparison of the results of hot soaking and cold soaking of myrtle fruit samples. It can be seen from Table 1 that the results of hot soaking and cold soaking using water and ethanol as solvents show that the content of extract measured by hot soaking is significantly higher than that by cold soaking.

[0046] Table 2 is a comparison of the results of hot soaking of myrtle fruit using different solvents. The samples were tested using ethanol, 50% ethanol, 75% ethanol, water, and anhydrous ethanol, respectively. The specific data are shown in Table 2.

[0047] According to the determination results, water is selected as the extraction solvent and the hot soaking method is used as the method for determining the extract of myrtle fruit.

[0048] Example 11: Standard curve and method verification for determination of quercetin content in Peach Blossom Tree medicinal material by liquid chromatography:

[0049] (1) Standard curve

[0050] Accurately weigh 11.79 mg of quercetin reference substance (batch number 111538-201105, calculated as 92.7%), place in a 50 mL brown volumetric flask, add methanol to dissolve and dilute to scale, shake well, and make a solution containing 218.5866 μg per 1 mL as the reference substance stock solution. Accurately pipette appropriate amount of the above reference substance stock solution to make quercetin concentrations of 1.0929, 10.9293, 27.3233, 54.6467, 78.3414, 97.9268, 122.4085, 153.0106 μg mL -1 Accurately pipette 10 μL of each of the above solutions, inject into the liquid chromatograph, and record the peak area. -1 ) as the horizontal axis, and the peak area integral value as the vertical axis for regression, the standard curve equation is: Y = 3.12 × 10 4 X-3.84×10 4 , R = 0.9998, at 1.0929 μg mL -1To 153.0106 μg mL -1 Within this range, the peak area of ​​quercetin had a good linear relationship with its concentration;

[0051] (2) Precision experiment

[0052] Take the quercetin reference solution, repeat the injection 6 times under the chromatographic conditions of Example 9, and record the peak area. The results show that the RSD of the quercetin peak area integral value is 0.06% (n=6), indicating that the precision of the instrument is good;

[0053] (3) Stability test

[0054] Take a sample of myrtle fruit (source: Pakistan, batch number Z30020018) to prepare a test solution, place it at room temperature for 0, 2, 4, 6, 8, 10, 12, 24, 30, 36, 48 hours, and measure it under the chromatographic conditions of Example 9, and record the peak area. The results show that the RSD of quercetin is 1.12% (n=11), indicating that the stability of the peak area integral value of quercetin determined by this method is good, and the measurement result within 48 hours is reliable;

[0055] (4) Repeatability experiment

[0056] Six parallel samples of the myrtle fruit sample (source: Pakistan, batch number Z30020018) were taken and prepared into test solution respectively. The sample was tested under the chromatographic conditions of Example 9 and the injection was repeated twice. The average peak area of ​​the reference substance was 1626103, and the RSD% of the sample content was 0.46%. The results are shown in Table 4. The results show that the method of the present invention has good repeatability for the determination of quercetin in myrtle fruit samples and can be used as a charge indicator for myrtle fruit medicinal materials;

[0057] (5) Recovery rate determination

[0058] Accurately weigh the myrtle fruit sample with a known content determined by the same method (source: Pakistan, batch number Z30020018, known content is 358.67 μg g -1 ) 2g, 9 portions in total, added at a concentration of 218.5866μg·mL -1 2 ml, 3 ml, and 4 ml of quercetin reference solution were used to prepare test solution, respectively, with a concentration of 54.64665 μg mL -1 The quercetin reference solution was injected with 10 μL each, and the recovery was calculated by the external standard method. The average peak area of ​​the quercetin reference was 1626103, the average recovery was 99.86%, and the RSD (%) was 1.48% (n=9);

[0059] (6) Determination of detection limit and quantification limit

[0060] A solution containing an appropriate concentration of quercetin reference substance was prepared and 10 μL was injected. When the signal-to-noise ratio (S / N) was 3, the minimum detection limit was 0.3279 μg·mL -1 ; When the signal-to-noise ratio (S / N) is 10, the limit of quantification is 1.0929 μg·mL -1 ;

[0061] (7) Durability test

[0062] Two parallel samples of myrtle fruit (source: Pakistan, batch number Z30020018) were taken and prepared into test solutions. The contents were determined by using three high performance liquid chromatographs from three different manufacturers and three different chromatographic columns. The results are shown in Table 5. The RSD% of the results was 1.27%, indicating that the quercetin (C 21 H 20 O 11 ) The content determination method has good robustness;

[0063] (8) Sample measurement

[0064] 15 batches of myrtle fruit samples were taken, crushed, and the powders (passed through a No. 3 sieve) were respectively prepared into test solutions, and the content was determined under the chromatographic conditions of Example 9. The content results are shown in Table 6. The content determination results of the above 15 batches of myrtle fruit ranged from 0.024% to 0.036%, with an average content of 0.032%. The content determination limit can be formulated according to the sample data; the content determination results between batches are not much different, and the method has good stability. The quercetin (C 21 H 20 O 11 ) content determination method can effectively control the intrinsic quality of myrtle fruit medicinal materials.

[0065] The present invention uses two thin layer chromatography to qualitatively identify the effective components in the myrtle fruit medicinal material; uses a water-soluble hot leaching method as a method for determining the medicinal material extract; and uses a high performance liquid chromatography method to determine the content of the effective components of the medicinal material. Among them, the thin layer identification method of the myrtle fruit medicinal material has clear spots and good separation effect; the average leaching rate of the extract method is 48.3%; the liquid chromatography method determines the content of quercetin in the medicinal material, which has the characteristics of good accuracy, simple sample processing, good stability, and strong specificity. It can be used as a detection method for the quality standard of myrtle fruit and can effectively control the intrinsic quality of the medicinal material. The present invention provides a reference basis for Chinese medicine researchers and lays a foundation for further in-depth research on myrtle fruit.

[0066] In summary, the method of the present invention is simple to operate, rapid, stable, reliable, accurate, and highly specific, and can be effectively used for the detection of myrtle fruit medicinal materials, providing technical guarantee for the further development of myrtle fruit medicinal material identification and intrinsic quality control, and laying a foundation for further in-depth research on myrtle fruit.

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Claims

1. A method for detecting myrtle fruit medicinal material, characterized in that Follow these steps: The first step is to identify gallic acid and ellagic acid in myrtle fruit by thin layer chromatography, the steps are: prepare the first test solution, prepare ellagic acid reference solution and gallic acid reference solution, respectively absorb the ellagic acid reference solution, gallic acid reference solution, and the first test solution, spot them on the same silica gel G thin layer plate, use toluene-ethyl formate-formic acid with a volume ratio of 5:5:0.4 as the developing agent, develop, dry, spray with an equal amount of a mixed solution of 2% ferric chloride solution and 2% potassium ferrocyanide solution, and inspect under sunlight. In the thin layer chromatography of the test sample, Spots of the same color appear at the corresponding positions of the thin-layer chromatography of the reference substance; wherein the preparation method of the first test solution is: take 1g to 5g of myrtle fruit powder, add 20mL to 30mL of 40% ethanol, heat and reflux for 30 minutes, filter, evaporate the filtrate to dryness, add 20mL of water to the residue after the filtrate is evaporated to dryness to dissolve, extract twice with 15mL to 30mL of ethyl acetate after the residue is dissolved, combine the extracts to obtain an ethyl acetate solution, evaporate to dryness, and dissolve the evaporated ethyl acetate solution with 1mL of ethyl acetate to obtain the first test solution; The second step is to identify quercetin and hyperoside in myrtle fruit by thin layer chromatography, the steps are: prepare a second test solution, prepare a quercetin reference solution and a hyperoside reference solution, respectively absorb the quercetin reference solution, the hyperoside reference solution, and the second test solution, spot them on the same silica gel G thin layer plate, use ethyl acetate-formic acid-water with a volume ratio of 8:1:1 as a developing solvent, develop, take out, dry, spray with 5% aluminum chloride ethanol solution, heat at 105°C for 2 minutes, and place under ultraviolet light. Under the fluorescent lamp, in the thin layer chromatogram of the test sample, a fluorescent spot of the same color appears at the corresponding position of the thin layer chromatogram of the reference sample; wherein, the preparation method of the second test solution is as follows: take 1g to 5g of myrtle fruit powder, add 15mL to 30mL of water, ultrasonically extract for 30 minutes, filter, discard the filtrate, add 40mL to 70mL of methanol to the filter residue, ultrasonically extract for 40 minutes, filter to obtain a methanol extract, evaporate the methanol extract to dryness, and then add 2mL of methanol to dissolve to obtain a second test solution; The third step is to determine the extracts in the myrtle fruit; The fourth step is to determine the content of quercetin in the myrtle fruit wood medicinal material.

2. The method for detecting the myrtle fruit medicinal material according to claim 1, wherein In the third step, water is used as the extraction solvent for the determination of the extract in the myrtle fruit, and the hot soaking method is used as the determination method for the extract in the myrtle fruit.

3. The detection method of the myrtle fruit medicinal material according to claim 1 or 2, characterized in that In the fourth step, the content of quercetin in the myrtle fruit wood medicinal material was determined by liquid chromatography.

4. The method for detecting the myrtle fruit medicinal material according to claim 3, wherein In the fourth step, the liquid chromatography conditions are as follows: the chromatographic column is a C18 chromatographic column, the chromatographic column filler particle size is 5 μm, the mobile phase is a mixture of acetonitrile and 0.1% formic acid in a volume ratio of 20:80, the detection wavelength is 256 nm, the column temperature is 30° C., and the injection volume is 10 μL.

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