Anti-aging and hair-toughening collagen peptide composition and preparation method thereof

Abstract

The invention provides an anti-aging and hair-toughening collagen peptide composition and a preparation method thereof, and belongs to the technical field of collagen peptides. The preparation process comprises the following steps: pretreating the raw materials; extraction and preparation of collagen peptide; preparation of the gallic acid-sodium hyaluronate grafted collagen peptide; preparing a haematococcus pluvialis composite microcapsule; pea seedling and opuntia ficus-indica-assisted bidirectional fermentation preparation; and preparing the collagen peptide composition. The composition disclosed by the invention contains gallic acid-sodium hyaluronate grafted collagen peptide, haematococcus pluvialis composite microcapsules, fermentation liquor, wheat germ powder and ginseng powder, and has anti-aging and hair-toughening effects.

Description

Technical Field

[0001] The present invention relates to the technical field of collagen peptide preparation, and in particular to an anti-aging and hair-toughening collagen peptide composition and a preparation method thereof. Background Art

[0002] With the improvement of people's living standards and the increase in demand for beauty and skin care, hair care products have gradually attracted widespread attention. In particular, a variety of hair care products have appeared on the market to address problems such as hair aging and fragility. At present, common hair care ingredients include natural plant extracts, proteins, peptides, etc. Among them, collagen peptides have been widely used in the field of hair care due to their good biocompatibility and moisturizing properties.

[0003] Although existing hair care products have improved the health of hair to a certain extent, there are still some shortcomings: many hair care products only use a single active ingredient, such as plant extracts or proteins, and their overall effect is limited. In addition, traditional collagen peptides have a large molecular weight and are difficult to be effectively absorbed by the scalp and hair follicles, resulting in low bioavailability. At the same time, most active ingredients are easily inactivated during storage and use, affecting the long-term effects of the product.

[0004] Therefore, we propose an anti-aging and tough hair collagen peptide composition and a preparation method thereof, aiming to solve the problems existing in the prior art. Summary of the invention

[0005] In view of the shortcomings of the prior art, the object of the present invention is to provide an anti-aging and hair-toughening collagen peptide composition and a preparation method thereof.

[0006] A method for preparing an anti-aging and hair-toughening collagen peptide composition comprises the following steps: S1: Pretreatment of raw materials, Silver carp skin is subjected to impurity removal, degreasing and decalcification treatment; S2: Extraction and preparation of collagen peptides, The collagen peptides were extracted by ultrasound combined with multi-step acid enzymatic hydrolysis; S3: Preparation of gallic acid-sodium hyaluronate grafted collagen peptide, Sodium hyaluronate is modified with gallic acid and then grafted onto collagen peptide to prepare gallic acid-sodium hyaluronate grafted collagen peptide; S4: Preparation of Haematococcus pluvialis composite microcapsules, Soy lecithin and soy protein isolate were used as wall materials to encapsulate Haematococcus pluvialis and rosemary essential oil emulsion, and then they were encapsulated with gum arabic and treated with low-temperature plasma to prepare Haematococcus pluvialis composite microcapsules. S5: Preparation of pea seedling and prickly pear cactus assisted bidirectional fermentation, The bidirectional fermentation of Schizophyllum and Dendrobium officinale was assisted by pea seedlings and Opuntia pomegranate. S6: Preparation of collagen peptide composition, 3-5 parts by weight of gallic acid-sodium hyaluronate grafted collagen peptide, 2-3 parts by weight of Haematococcus pluvialis composite microcapsules, 6-8 parts by weight of fermentation liquid, 1-2 parts by weight of wheat germ powder and 1-2 parts by weight of ginseng powder are mixed to obtain a collagen peptide composition.

[0007] Furthermore, step S1 of pre-processing raw materials specifically includes the following steps: S1.1: silver carp skin is recovered from the waste materials of the surimi factory, the skin is cut into pieces, the cut pieces are placed in a vacuum freeze dryer and dried for 48-50 hours, the freeze-dried pieces are placed in a sealed bag, and placed in a dryer for later use; S1.2: The chopped fish skin is homogenized and stirred in a 0.1-0.2 mol / L sodium hydroxide solution for 5-10 min, then soaked for 6-7 h, the alkali solution is replaced every 2-3 h, and finally washed to neutrality and filtered to obtain a fish skin mixture after removing impurities such as protein and pigment; S1.3: Soak 1-2 parts by weight of the fish skin mixture in 10-12 parts by weight of a 10-12wt% isopropanol solution for 9-12 hours, replace the solution every 3-4 hours, then rinse and filter with distilled water for 2-3 times, then put it into 10-12 parts by weight of a 0.3mol / L EDTA solution and soak it for 9-12 hours, then rinse and filter with distilled water for 2-3 times, and centrifuge to obtain the pretreated raw material.

[0008] Furthermore, step S2, extraction and preparation of collagen peptides, specifically comprises the following steps: S2.1: Soak 3-5 parts by weight of the pretreated raw material in 15-20 parts by weight of 0.5-0.8 mol / L lactic acid solution, homogenize in an ice bath for 5-8 minutes, ultrasonicate at 4-5°C, frequency 60-70 kHz, time 15-20 minutes, power 100-200 W, add pepsin at 20-22 U / g, and hydrolyze for 10-12 hours to obtain the first hydrolyzate; S2.2: The first hydrolyzate is ultrasonicated at 30-40°C, frequency 60-70kHz, time 15-20min, power 100-200W, 10-12U / g papain is added, and the hydrolysis is carried out for 10-12h, and then 10-12U / g bromelain is added, and the hydrolysis is carried out for 10-12h to obtain the hydrolyzate; S2.3: The enzymatic hydrolysis product is heated to 90-95°C for inactivation and maintained for 20-30 minutes, then cooled to 35-40°C and filtered. The filtrate is ultrafiltered using a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and the permeate is freeze-dried to obtain collagen peptides.

[0009] Furthermore, step S3 of preparing gallic acid-sodium hyaluronate grafted collagen peptide specifically comprises the following steps: S3.1: Add 2-3 parts by weight of sodium hyaluronate to 30-50 parts by weight of 2-3% formic acid solution, stir and mix for 10-12 minutes, then add 0.1-0.2 parts by weight of Vc and 1-2 parts by weight of gallic acid, stir and react for 20-30 minutes, then add 8-10 parts by weight of hydrogen peroxide, stir and mix for 20-24 hours, and then perform ultrafiltration with a hollow fiber membrane with a molecular weight cutoff of 10000Da, and freeze-dry the filtrate to obtain gallic acid-sodium hyaluronate; S3.2: Dissolve 2-3 parts by weight of gallic acid-sodium hyaluronate in 30-50 parts by weight of deionized water, then add 5-8 parts by weight of collagen peptide, stir and mix, then add 0.2-0.5 parts by weight of transglutaminase, react for 5-6 hours, then raise the temperature to 30-50°C, react for 15-20 minutes, cool after the reaction is completed, ultrafilter with a hollow fiber membrane with a molecular weight cutoff of 10,000Da, and freeze-dry the filtrate to obtain gallic acid-sodium hyaluronate grafted collagen peptide.

[0010] Furthermore, step S4 of preparing the composite microcapsules of Haematococcus pluvialis specifically comprises the following steps: S4.1: dissolving 1-2 parts by weight of soy lecithin and 1-2 parts by weight of soy protein isolate in 50-60 parts by weight of deionized water at 40-50° C. to obtain a wall material solution; S4.2: 1-2 parts by weight of Haematococcus pluvialis, 0.5-0.8 parts by weight of Tween 80 and 30-50 parts by weight of deionized water are mixed and sheared and stirred at 8000-9000 r / min for 10-12 min to obtain a Haematococcus pluvialis emulsion; 1-2 parts by weight of rosemary essential oil, 0.5-0.8 parts by weight of Tween 80 and 30-50 parts by weight of deionized water are mixed and sheared and stirred at 8000-9000 r / min for 10-12 min to obtain a rosemary essential oil emulsion; S4.3: 2-3 parts by weight of Haematococcus pluvialis emulsion and 1-2 parts by weight of wall material solution are mixed, and then the pH is adjusted to 11-12 with a sodium hydroxide solution, and after ultrasonic treatment, the pH is adjusted to neutral with hydrogen chloride, and the mixture is stirred at 5000-6000 r / min for 30-40 min to obtain a mixed solution I; S4.4: 2-3 parts by weight of rosemary essential oil emulsion and 1-2 parts by weight of wall material solution are mixed, and then the pH is adjusted to 11-12 with sodium hydroxide solution, and after ultrasonic treatment, the pH is adjusted to neutral with hydrogen chloride, and the mixture is stirred at 5000-6000 r / min for 30-40 min to obtain a mixed solution II; S4.5: Mix the mixed solution I and the mixed solution II, add 2-3 parts by weight of 1-2wt% gum arabic solution, and then stir and mix at 200-300r / min for 20-30min, transfer the mixed solution into a quartz reactor with 1-1.2A, 50-60W low-temperature plasma for 60-70s treatment, spray dry the treated mixed solution to obtain Haematococcus pluvialis composite microcapsules.

[0011] Furthermore, step S5 of preparing pea seedlings and prickly pear cactus assisted bidirectional fermentation specifically comprises the following steps: S5.1: Dry the Dendrobium officinale, pea seedlings, and Opuntia pyri in a constant temperature oven at 50-60°C to constant weight, grind them with a traditional Chinese medicine grinder, and pass them through a 30-40 mesh sieve to obtain a mixed powder, seal the mixed powder in a ziplock bag, and store it in a dry state; S5.2: 3-5 g of the mixed powder, 2-3 g of yeast extract, 0.5-0.8 g of magnesium sulfate, 1-2 g of potassium dihydrogen phosphate, 30-50 g of glucose, and 1-1.2 L of deionized water are mixed to obtain a culture medium, and the culture medium is adjusted to 6-6.5 to obtain a culture medium; S5.3: After activating the Schizophyllum, culture it and inoculate the obtained Schizophyllum seed liquid into the culture medium, and then ferment it at 25-26°C and 150-160r / min for 40-48h. Centrifuge the fermentation product to obtain the fermentation liquid.

[0012] Furthermore, step S6 of preparing the collagen peptide composition specifically comprises the following steps: S6.1: adding 3-5 parts by weight of gallic acid-sodium hyaluronate grafted collagen peptide and 2-3 parts by weight of Haematococcus pluvialis composite microcapsules to 6-8 parts by weight of fermentation broth, stirring and mixing to obtain a mixture; S6.2: Add 1-2 parts by weight of wheat germ powder and 1-2 parts by weight of ginseng powder to the mixture, and stir and mix at 200-300 r / min for 20-30 min to obtain a collagen peptide composition.

[0013] Furthermore, in step S1.2, the ratio of fish skin to sodium hydroxide solution is 1:25-27 w / v.

[0014] Furthermore, the parameters of the ultrasonic treatment in steps S4.3 and S4.4 are 200-230 W, 10-12 kHz, and 3-5 min.

[0015] An anti-aging and hair-toughening collagen peptide composition is prepared by any of the methods for preparing an anti-aging and hair-toughening collagen peptide composition.

[0016] Compared with the prior art, the present invention has at least the following beneficial effects: 1. The anti-aging and hair-toughening collagen peptide composition of the present invention adopts multiple active ingredients such as Haematococcus pluvialis, rosemary essential oil, pea seedlings, prickly pear cactus and gallic acid-sodium hyaluronate grafted collagen peptide, etc., with strong comprehensive effect. In addition, the bioavailability of collagen peptide is improved by grafting collagen peptide with gallic acid-sodium hyaluronate. At the same time, it is not easy to inactivate during storage and use after modification and grafting. In addition, Haematococcus pluvialis and rosemary essential oil are double-compounded to improve their stability and improve the long-term anti-aging and hair-toughening effect of the composition.

[0017] 2. The present invention grafts collagen peptides after sodium hyaluronate is modified by gallic acid. After sodium hyaluronate modified by gallic acid is grafted with fish bone collagen peptides, chemical bonds are formed between the components. Compared with simple physical mixing, this bond is stronger and less likely to separate or degrade during storage and use. Sodium hyaluronate itself has good hydrophilicity and can attract moisture and keep it on the surface of skin and hair. Gallic acid has a certain lipophilicity. After grafting, the molecules of gallic acid-sodium hyaluronate grafted collagen peptides have both hydrophilicity and lipophilicity, and can better adhere to skin and hair. The balance interacts with the lipid layer and water environment of the hair. This balance makes it easier for gallic acid-sodium hyaluronate grafted collagen peptide to penetrate the barrier of skin and hair, increasing the possibility of being absorbed by cells, thereby effectively improving bioavailability. Gallic acid has an antioxidant effect, sodium hyaluronate has a moisturizing effect, and fish bone collagen peptide has a nourishing and repairing effect. Through the grafting reaction, these ingredients can work synergistically at the molecular level and improve multiple hair problems at the same time. They can more effectively promote hair growth and toughness, reduce hair aging, and avoid hair loss and gray hair.

[0018] 3. The present invention adopts pea seedlings and prickly pear cactus to assist in the bidirectional fermentation of Dendrobium officinale and Schizophyllum, and the fermentation liquid contains a variety of active ingredients, so that the fermentation liquid has the ability of anti-oxidation and scavenging free radicals. In addition, pea seedlings and prickly pear cactus are used for auxiliary fermentation. Pea seedlings and prickly pear cactus are rich in nutrients such as carbohydrates, proteins, vitamins, etc., which provide a good matrix for the growth and metabolism of Schizophyllum. Schizophyllum will use these matrices for growth and metabolism during the fermentation process to produce various metabolites, thereby increasing the antioxidant properties of the fermentation liquid and inhibiting the levels of elastase and tyrosinase. At the same time, pea seedlings and prickly pear cactus themselves promote hair growth and have anti-aging and hair toughening effects. Therefore, adding the composition of the fermentation liquid can further improve the product's effect of promoting hair growth and toughness and reducing hair aging.

[0019] 4. The present invention adopts soybean lecithin and soybean protein isolate as wall materials to encapsulate Haematococcus pluvialis and rosemary essential oil. Soybean lecithin is an amphiphilic substance, that is, it has both hydrophilicity and lipophilicity, so that the prepared Haematococcus pluvialis composite microcapsules can better act on the scalp. Thereafter, ultrasonic modification is performed, and then a gum arabic solution is used for composite encapsulation, and finally low-temperature plasma treatment is performed. The ultrasonic treatment can make the encapsulation material more evenly encapsulated on the surface of Haematococcus pluvialis and rosemary essential oil, thereby improving the compactness and uniformity of the encapsulation. The cavitation and mechanical effect of the ultrasound can promote the interaction between the wall material and the core material, enhance the stability of the encapsulation, and improve the embedding rate. The embedding rate can be improved after the low-temperature plasma treatment, and the dual-core encapsulation after the ultrasonic treatment combined with the low-temperature plasma treatment can promote the antioxidant effect of Haematococcus pluvialis and rosemary essential oil and prolong the antioxidant effect, so that the Haematococcus pluvialis composite microcapsules can achieve a long-lasting antioxidant effect, thereby improving the lasting effect of the composition in resisting aging and toughening hair. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the invention and, together with the description, further serve to explain the principles of the invention and to enable those skilled in the relevant art to make and use the invention.

[0021] Figure 1 A flow chart of a method for preparing an anti-aging and hair-toughening collagen peptide composition used in an embodiment of the present invention; Figure 2 This is an example diagram of the results of multi-core double-wall microcapsules prepared in an embodiment of the present invention. DETAILED DESCRIPTION

[0022] The following is a detailed description of the preparation method of the anti-aging and toughening hair collagen peptide composition provided by the present invention in conjunction with the accompanying drawings and specific examples. At the same time, it is explained here that in order to make the examples more detailed, the following examples are the best and preferred examples, and those skilled in the art may also adopt other alternative methods to implement some known technologies; and the accompanying drawings are only for more specific description of the examples, and are not intended to specifically limit the present invention.

[0023] Example 1 A method for preparing an anti-aging and hair-toughening collagen peptide composition, such as Figure 1 As shown, the following steps are included: S1: Pretreatment of raw materials S1.1: silver carp skin is recovered from the waste materials of the surimi factory, the skin is cut into pieces, the cut pieces are placed in a vacuum freeze dryer and dried for 48 hours, the freeze-dried pieces are placed in a sealed bag, and placed in a dryer for later use; S1.2: The chopped fish skin is placed in a 0.1 mol / L sodium hydroxide solution, wherein the ratio of fish skin to sodium hydroxide solution is 1:25 w / v, homogenized and stirred for 5 min, and then soaked for 6 h, the alkali solution is replaced every 2 h, and finally washed to neutrality and filtered to obtain a fish skin mixture after removing impurities such as protein and pigment; S1.3: 1 part by weight of the fish skin mixture is soaked in 10 parts by weight of a 10wt% isopropanol solution for 9 hours, the solution is replaced every 3 hours, then filtered twice with distilled water, then placed in 10 parts by weight of a 0.3mol / L EDTA solution and soaked for 9 hours, then filtered twice with distilled water, and centrifuged to obtain the pretreated raw material; S2: Extraction and preparation of collagen peptides S2.1: 3 parts by weight of the pretreated raw material were soaked in 15 parts by weight of 0.5 mol / L lactic acid solution, homogenized in an ice bath for 5 min, and then sonicated at 4°C, with a frequency of 60 kHz, a time of 15 min, a power of 100 W, and pepsin was added at a rate of 20 U / g, and enzymolysis was performed for 10 h to obtain the first enzymolysis product; S2.2: The first hydrolyzate was subjected to ultrasonic treatment at 30°C, a frequency of 60 kHz, a time of 15 min, a power of 100 W, 10 U / g of papain was added, and the hydrolysis was carried out for 10 h, and then 10 U / g of bromelain was added, and the hydrolysis was carried out for 10 h to obtain the hydrolyzate; S2.3: The enzymatic hydrolysate was heated to 90°C for inactivation and maintained for 20 min, then cooled to 35°C and filtered. The filtrate was ultrafiltered using a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and the permeate was freeze-dried to obtain collagen peptides. S3: Preparation of Gallic Acid-Sodium Hyaluronate Grafted Collagen Peptide S3.1: Add 2 parts by weight of sodium hyaluronate to 30 parts by weight of 2% formic acid solution, stir and mix for 10 minutes, then add 0.1 parts by weight of Vc and 1 part by weight of gallic acid, stir and react for 20 minutes, then add 8 parts by weight of hydrogen peroxide, stir and mix for 20 hours, and then perform ultrafiltration with a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and freeze-dry the filtrate to obtain gallic acid-sodium hyaluronate; S3.2: Dissolve 2 parts by weight of gallic acid-sodium hyaluronate in 30 parts by weight of deionized water, then add 5 parts by weight of collagen peptide, stir and mix, then add 0.2 parts by weight of transglutaminase, react for 5 hours, then heat to 30°C, react for 15 minutes, cool after the reaction is completed, ultrafilter with a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and freeze-dry the filtrate to obtain gallic acid-sodium hyaluronate grafted collagen peptide; S4: Preparation of Haematococcus pluvialis composite microcapsules S4.1: dissolving 1 part by weight of soybean lecithin and 1 part by weight of isolated soybean protein in 50 parts by weight of deionized water at 40° C. to obtain a wall material solution; S4.2: 1 part by weight of Haematococcus pluvialis, 0.5 part by weight of Tween 80 and 30 parts by weight of deionized water were mixed and sheared and stirred at 8000 r / min for 10 min to obtain a Haematococcus pluvialis emulsion; 1 part by weight of rosemary essential oil, 0.5 part by weight of Tween 80 and 30 parts by weight of deionized water were mixed and sheared and stirred at 8000 r / min for 10 min to obtain a rosemary essential oil emulsion; S4.3: 2 parts by weight of Haematococcus pluvialis emulsion and 1 part by weight of wall material solution were mixed, and then the pH was adjusted to 11 by sodium hydroxide solution, and ultrasonic treatment was performed at 200W, 10kHz for 3 minutes, and then adjusted to neutrality with hydrogen chloride, and stirred and mixed at 5000r / min for 30 minutes to obtain mixed solution I; S4.4: 2 parts by weight of rosemary essential oil emulsion and 1 part by weight of wall material solution were mixed, and then the pH was adjusted to 11 with sodium hydroxide solution, and ultrasonic treatment was performed at 200 W and 10 kHz for 3 min, and then adjusted to neutral with hydrogen chloride, and stirred and mixed at 5000 r / min for 30 min to obtain a mixed solution II; S4.5: Mix the mixed solution I and the mixed solution II, add 2 parts by weight of 1 wt% gum arabic solution, and then stir and mix at 200 r / min for 20 min. Transfer the mixed solution into a quartz reactor with 1A, 50W low-temperature plasma for 60 s treatment. Spray dry the treated mixed solution to obtain Haematococcus pluvialis composite microcapsules. The structure of the Haematococcus pluvialis composite microcapsules is as follows: Figure 2 ; S5: Preparation of pea seedling and prickly pear cactus assisted bidirectional fermentation S5.1: Dry the Dendrobium officinale, pea seedlings, and Opuntia pyri in a constant temperature oven at 50°C to constant weight, grind them with a traditional Chinese medicine grinder, and pass them through a 30-mesh sieve to obtain a mixed powder, seal the mixed powder in a ziplock bag, and store it in a dry place; S5.2: 3 g of the mixed powder, 2 g of yeast extract, 0.5 g of magnesium sulfate, 1 g of potassium dihydrogen phosphate, 30 g of glucose, and 1 L of deionized water are mixed to obtain a culture medium, and the culture medium is adjusted to 6 to obtain a culture medium; S5.3: activating the Schizophyllum and culturing it, inoculating the obtained Schizophyllum seed liquid into the culture medium, and then fermenting it at 25°C and 150r / min for 40h, centrifuging the fermentation product to obtain a fermentation liquid; Among them, the strain number of Schizophyllum is CICC 2591, and its Latin name is Schizophyllum commune; S6: Preparation of collagen peptide composition S6.1: Add 3 parts by weight of gallic acid-sodium hyaluronate grafted collagen peptide and 2 parts by weight of Haematococcus pluvialis composite microcapsules to 6 parts by weight of fermentation broth, stir and mix evenly to obtain a mixture; S6.2: Add 1 part by weight of wheat germ powder and 1 part by weight of ginseng powder to the mixture, and stir and mix at 200 r / min for 20 minutes to obtain a collagen peptide composition.

[0024] Example 2 A method for preparing an anti-aging and hair-toughening collagen peptide composition, such as Figure 1 As shown, the following steps are included: S1: Pretreatment of raw materials S1.1: silver carp skin is recovered from the waste materials of the surimi factory, the skin is cut into pieces, the cut pieces are placed in a vacuum freeze dryer and dried for 48 hours, the freeze-dried pieces are placed in a sealed bag, and placed in a dryer for later use; S1.2: The chopped fish skin is placed in a 0.2 mol / L sodium hydroxide solution, wherein the ratio of fish skin to sodium hydroxide solution is 1:27 w / v, homogenized and stirred for 5 min, and then soaked for 6 h, the alkali solution is replaced every 2 h, and finally washed to neutrality and filtered to obtain a fish skin mixture after removing impurities such as protein and pigment; S1.3: 2 parts by weight of the fish skin mixture are soaked in 12 parts by weight of a 12wt% isopropanol solution for 9 hours, the solution is replaced every 3 hours, then filtered twice with distilled water, then placed in 12 parts by weight of a 0.3mol / L EDTA solution and soaked for 9 hours, then filtered twice with distilled water, and centrifuged to obtain the pretreated raw material; S2: Extraction and preparation of collagen peptides S2.1: 5 parts by weight of the pretreated raw material were soaked in 20 parts by weight of 0.8 mol / L lactic acid solution, homogenized in an ice bath for 5 min, and then sonicated at 4°C, with a frequency of 60 kHz, a time of 15 min, a power of 100 W, and pepsin was added at a rate of 22 U / g, and enzymolysis was performed for 10 h to obtain the first enzymolysis product; S2.2: The first hydrolyzate was subjected to ultrasonic treatment at 30°C, a frequency of 60 kHz, a time of 15 min, a power of 100 W, 12 U / g of papain was added, and the hydrolysis was carried out for 10 h, and then 12 U / g of bromelain was added, and the hydrolysis was carried out for 10 h to obtain the hydrolyzate; S2.3: The enzymatic hydrolysate was heated to 90°C for inactivation and maintained for 20 min, then cooled to 35°C and filtered. The filtrate was ultrafiltered using a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and the permeate was freeze-dried to obtain collagen peptides. S3: Preparation of Gallic Acid-Sodium Hyaluronate Grafted Collagen Peptide S3.1: 3 parts by weight of sodium hyaluronate are added to 50 parts by weight of 3% formic acid solution, and the mixture is stirred for 10 minutes. Then, 0.2 parts by weight of Vc and 2 parts by weight of gallic acid are added, and the mixture is stirred for 20 minutes. Then, 10 parts by weight of hydrogen peroxide are added, and the mixture is stirred for 20 hours. The mixture is then ultrafiltered using a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and the filtrate is freeze-dried to obtain gallic acid-sodium hyaluronate; S3.2: 3 parts by weight of gallic acid-sodium hyaluronate were dissolved in 50 parts by weight of deionized water, and then 8 parts by weight of collagen peptide were added. After stirring and mixing, 0.5 parts by weight of transglutaminase were added, and the reaction was continued for 5 hours. Then, the temperature was raised to 30°C and the reaction was continued for 15 minutes. After the reaction was completed, the mixture was cooled, ultrafiltered with a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and the filtrate was freeze-dried to obtain gallic acid-sodium hyaluronate grafted collagen peptide; S4: Preparation of Haematococcus pluvialis composite microcapsules S4.1: dissolving 2 parts by weight of soybean lecithin and 2 parts by weight of isolated soybean protein in 60 parts by weight of deionized water at 40° C. to obtain a wall material solution; S4.2: 2 parts by weight of Haematococcus pluvialis, 0.8 parts by weight of Tween 80 and 50 parts by weight of deionized water were mixed and sheared and stirred at 8000 r / min for 10 min to obtain a Haematococcus pluvialis emulsion; 2 parts by weight of rosemary essential oil, 0.8 parts by weight of Tween 80 and 50 parts by weight of deionized water were mixed and sheared and stirred at 8000 r / min for 10 min to obtain a rosemary essential oil emulsion; S4.3: 3 parts by weight of Haematococcus pluvialis emulsion and 2 parts by weight of wall material solution were mixed, and then the pH was adjusted to 12 with a sodium hydroxide solution, and ultrasonic treatment was performed at 200 W and 10 kHz for 3 min, and then adjusted to neutral with hydrogen chloride, and stirred and mixed at 5000 r / min for 30 min to obtain a mixed solution I; S4.4: 3 parts by weight of rosemary essential oil emulsion and 2 parts by weight of wall material solution were mixed, and then the pH was adjusted to 12 with sodium hydroxide solution, and ultrasonic treatment was performed at 200 W and 10 kHz for 3 min, and then adjusted to neutral with hydrogen chloride, and stirred and mixed at 6000 r / min for 40 min to obtain a mixed solution II; S4.5: Mix the mixed solution I and the mixed solution II, add 3 parts by weight of 2 wt% gum arabic solution, stir and mix at 200 r / min for 20 min, transfer the mixed solution into a quartz reactor with 1A, 50W low-temperature plasma for 60 s, spray dry the treated mixed solution, and obtain Haematococcus pluvialis composite microcapsules; S5: Preparation of pea seedling and prickly pear cactus assisted bidirectional fermentation S5.1: Dry the Dendrobium officinale, pea seedlings, and Opuntia pyri in a constant temperature oven at 50°C to constant weight, grind them with a traditional Chinese medicine grinder, and pass them through a 30-mesh sieve to obtain a mixed powder, seal the mixed powder in a ziplock bag, and store it in a dry place; S5.2: 5 g of mixed powder, 3 g of yeast extract, 0.8 g of magnesium sulfate, 2 g of potassium dihydrogen phosphate, 50 g of glucose, and 1.2 L of deionized water were mixed to obtain a culture medium, and the culture medium was adjusted to 6.5 to obtain a culture medium; S5.3: activating the Schizophyllum and culturing it, inoculating the obtained Schizophyllum seed liquid into the culture medium, and then fermenting it at 25°C and 150r / min for 40h, centrifuging the fermentation product to obtain a fermentation liquid; Among them, the strain number of Schizophyllum is CICC 2591, and its Latin name is Schizophyllum commune; S6: Preparation of collagen peptide composition S6.1: adding 5 parts by weight of gallic acid-sodium hyaluronate grafted collagen peptide and 3 parts by weight of Haematococcus pluvialis composite microcapsules to 8 parts by weight of the fermentation broth, stirring and mixing to obtain a mixture; S6.2: Add 2 parts by weight of wheat germ powder and 2 parts by weight of ginseng powder to the mixture, and stir and mix at 200 r / min for 20 minutes to obtain a collagen peptide composition.

[0025] Example 3 A method for preparing an anti-aging and hair-toughening collagen peptide composition, such as Figure 1 As shown, the following steps are included: S1: Pretreatment of raw materials S1.1: silver carp skin is recovered from the waste materials of the surimi factory, the skin is cut into pieces, the cut pieces are placed in a vacuum freeze dryer and dried for 50 hours, the freeze-dried pieces are placed in a sealed bag, and placed in a dryer for later use; S1.2: The chopped fish skin is placed in a 0.1 mol / L sodium hydroxide solution, wherein the ratio of fish skin to sodium hydroxide solution is 1:25 w / v, homogenized and stirred for 10 min, and then soaked for 7 h, the alkali solution is replaced every 3 h, and finally washed to neutrality and filtered to obtain a fish skin mixture after removing impurities such as protein and pigment; S1.3: 1 part by weight of the fish skin mixture is soaked in 12 parts by weight of a 10wt% isopropanol solution for 12 hours, the solution is replaced every 4 hours, then filtered 3 times with distilled water, then placed in 10 parts by weight of a 0.3mol / L EDTA solution and soaked for 12 hours, then filtered 3 times with distilled water, and centrifuged to obtain the pretreated raw material; S2: Extraction and preparation of collagen peptides S2.1: 3 parts by weight of the pretreated raw material were soaked in 15 parts by weight of 0.5 mol / L lactic acid solution, homogenized in an ice bath for 8 min, and then sonicated at 5°C, with a frequency of 70 kHz, a time of 20 min, a power of 200 W, and pepsin was added at a rate of 20 U / g, and enzymolysis was performed for 12 h to obtain the first enzymolysis product; S2.2: The first hydrolyzate was subjected to ultrasonic treatment at 40°C, a frequency of 70 kHz, a time of 20 min, a power of 200 W, 10 U / g of papain was added, and the hydrolysis was carried out for 12 h, and then 10 U / g of bromelain was added, and the hydrolysis was carried out for 12 h to obtain the hydrolyzate; S2.3: The enzymatic hydrolysate was heated to 95°C for inactivation and maintained for 30 min, then cooled to 40°C and filtered. The filtrate was ultrafiltered using a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and the permeate was freeze-dried to obtain collagen peptides. S3: Preparation of Gallic Acid-Sodium Hyaluronate Grafted Collagen Peptide S3.1: Add 2 parts by weight of sodium hyaluronate to 30 parts by weight of 2% formic acid solution, stir and mix for 12 minutes, then add 0.1 parts by weight of Vc and 1 part by weight of gallic acid, stir and react for 30 minutes, then add 8 parts by weight of hydrogen peroxide, stir and mix for 24 hours, and then perform ultrafiltration with a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and freeze-dry the filtrate to obtain gallic acid-sodium hyaluronate; S3.2: Dissolve 2 parts by weight of gallic acid-sodium hyaluronate in 30 parts by weight of deionized water, then add 5 parts by weight of collagen peptide, stir and mix, then add 0.2 parts by weight of transglutaminase, react for 6 hours, then heat to 50°C, react for 20 minutes, cool after the reaction is completed, ultrafilter with a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and freeze-dry the filtrate to obtain gallic acid-sodium hyaluronate grafted collagen peptide; S4: Preparation of Haematococcus pluvialis composite microcapsules S4.1: dissolving 1 part by weight of soybean lecithin and 1 part by weight of isolated soybean protein in 60 parts by weight of deionized water at 50° C. to obtain a wall material solution; S4.2: 1 part by weight of Haematococcus pluvialis, 0.5 part by weight of Tween 80 and 30 parts by weight of deionized water were mixed and sheared and stirred at 9000 r / min for 12 min to obtain a Haematococcus pluvialis emulsion; 1 part by weight of rosemary essential oil, 0.5 part by weight of Tween 80 and 30 parts by weight of deionized water were mixed and sheared and stirred at 9000 r / min for 12 min to obtain a rosemary essential oil emulsion; S4.3: 2 parts by weight of Haematococcus pluvialis emulsion and 1 part by weight of wall material solution were mixed, and then the pH was adjusted to 11 by sodium hydroxide solution, and ultrasonic treatment was performed at 230W, 12kHz for 5 minutes, and then adjusted to neutrality with hydrogen chloride, and stirred and mixed at 6000r / min for 40 minutes to obtain mixed solution I; S4.4: 2 parts by weight of rosemary essential oil emulsion and 1 part by weight of wall material solution were mixed, and then the pH was adjusted to 11 with sodium hydroxide solution, and ultrasonic treatment was performed at 230W, 12kHz for 5 minutes, and then adjusted to neutral with hydrogen chloride, and stirred and mixed at 6000r / min for 40 minutes to obtain mixed solution II; S4.5: Mix the mixed solution I and the mixed solution II, add 2 parts by weight of 1 wt% gum arabic solution, stir and mix at 300 r / min for 30 min, transfer the mixed solution into a quartz reactor with 1.2A, 60W low-temperature plasma for 70 s, spray dry the treated mixed solution, and obtain Haematococcus pluvialis composite microcapsules; S5: Preparation of pea seedling and prickly pear cactus assisted bidirectional fermentation S5.1: Dry the Dendrobium officinale, pea seedlings, and Opuntia pyri in a constant temperature oven at 60°C to constant weight, grind them with a traditional Chinese medicine grinder, and pass them through a 40-mesh sieve to obtain a mixed powder, seal the mixed powder in a ziplock bag, and store it in a dry state; S5.2: 3 g of the mixed powder, 2 g of yeast extract, 0.5 g of magnesium sulfate, 1 g of potassium dihydrogen phosphate, 30 g of glucose, and 1 L of deionized water are mixed to obtain a culture medium, and the culture medium is adjusted to 6 to obtain a culture medium; S5.3: activating the Schizophyllum and culturing it, inoculating the obtained Schizophyllum seed liquid into the culture medium, and then fermenting it at 26°C and 160r / min for 48h, centrifuging the fermentation product to obtain a fermentation liquid; Among them, the strain number of Schizophyllum is CICC 2591, and its Latin name is Schizophyllum commune; S6: Preparation of collagen peptide composition S6.1: Add 3 parts by weight of gallic acid-sodium hyaluronate grafted collagen peptide and 2 parts by weight of Haematococcus pluvialis composite microcapsules to 6 parts by weight of fermentation broth, stir and mix evenly to obtain a mixture; S6.2: Add 1 part by weight of wheat germ powder and 1 part by weight of ginseng powder to the mixture, and stir and mix at 300 r / min for 30 minutes to obtain a collagen peptide composition.

[0026] Determination of anti-DPPH ability: The anti-DPPH· ability of the collagen peptide composition finally obtained in Examples 1-3 was measured, and the decrease in the ultraviolet absorption peak after adding the collagen peptide composition was used to represent its scavenging ability against DPPH·.

[0027] Accurately weigh 4 mg DPPH and add 50 mL anhydrous ethanol to prepare a DPPH solution with a concentration of 0.2 mmol / L. Use a UV-1600 ultraviolet-visible spectrophotometer to measure the absorbance of the solution and collect the absorption peak of the solution at 517 nm.

[0028] Accurately weigh the collagen peptide composition obtained in Examples 1-3 in a conical flask, dilute it to 500 mg / L with anhydrous ethanol, and use it as the sample solution to be tested. Take 5 mL of the sample solution to be tested and 5 mL of DPPH·solution, mix them, and react in the dark for 30 minutes. Using 95% ethanol as a reference object, measure the absorbance of the mixed solution at a wavelength of 517 nm, and record the result as A0. Take 5 mL of the sample solution to be tested and mix it with 5 mL of 95% ethanol, and react in the dark for 30 minutes. Using 95% ethanol as a reference object, measure the absorbance of the mixed solution at a wavelength of 517 nm, and record the result as A1. Take 5 mL of 95% ethanol and 5 mL of DPPH·solution, mix them, and react in the dark for 30 minutes. Using 95% ethanol as a reference object, measure the absorbance of the mixed solution at a wavelength of 517 nm, and record the result as A2.

[0029] DPPH clearance rate = [1-(A0-A1) / A2] × 100%.

[0030] The results of the DPPH resistance test are shown in Table 1.

[0031] Breaking strength and scalp health determination: The collagen peptide composition is added into deionized water, lauryl surfactant and coconut oil-based surfactant, and then tea saponin, guar gum hydroxypropyltrimethylammonium chloride and softener are added, and the mixture is stirred evenly to obtain an anti-aging and tough hair shampoo.

[0032] Hair Breaking Strength: Use a tensile testing machine to measure the hair's ability to resist stretching after shampooing.

[0033] Scalp health: Dermatologists use a skin scoring system to score the scalp condition out of 10 points, including observation of scalp dryness, erythema and inflammation.

[0034] Testing process: Each shampoo of Examples 1-3 was applied to 30 volunteers, and the baseline status of hair and scalp was evaluated for all test subjects before use.

[0035] Wash your hair once a day for two weeks and reassess your hair and scalp at pre-set time points during and after washes.

[0036] Please refer to Table 1 for the test results.

[0037] Table 1. Example 1-3 DPPH clearance, anti-breaking strength and scalp health test results

[0038] From the data in Table 1, it can be seen that the collagen peptide composition prepared by the present invention using multiple active ingredients such as Haematococcus pluvialis, rosemary essential oil, pea seedlings, prickly pear cactus and gallic acid-sodium hyaluronate grafted collagen peptide has good DPPH scavenging ability, and the collagen peptide composition has good antioxidant properties, which can effectively help cells restore vitality, promote cell repair, and restore damaged hair to natural health. In addition, after shampooing with the shampoo prepared by the collagen peptide composition of the present invention, the anti-breaking strength of the hair is 132-137N, the hair has good toughness, and the scalp can be kept in a healthy state, indicating that the collagen peptide composition prepared by the present invention has a good anti-aging and hair toughening effect.

[0039] The collagen peptide composition prepared by the present invention can also be used in other anti-aging and hair strengthening products.

[0040] Comparative Example 1 Compared with Example 1, the difference of Comparative Example 1 is that Comparative Example 1 removes step S3, and replaces the gallic acid-sodium hyaluronate grafted collagen peptide in step S6.1 with 3-5 parts by weight of gallic acid, 3-5 parts by weight of sodium hyaluronate and 3-5 parts by weight of collagen peptide, and the remaining steps remain unchanged to prepare the collagen peptide composition, which is recorded as Comparative Example 1.

[0041] Comparative Example 2 Compared with Example 1, the difference of Comparative Example 2 is that the pea seedlings and prickly pear cactus in step S5.1 are removed in Comparative Example 2, and the collagen peptide composition is prepared without changing the other steps, which is recorded as Comparative Example 2.

[0042] Comparative Example 3 Compared with Example 1, the difference of Comparative Example 3 is that Comparative Example 3 removes "then adjust the pH to 11 with sodium hydroxide solution, ultrasonically treat at 200W, 10kHz for 3 minutes, and then adjust to neutral with hydrogen chloride" in steps S4.3 and S4.4, and the remaining steps remain unchanged to prepare the collagen peptide composition, which is recorded as Comparative Example 3.

[0043] Comparative Example 4 Compared with Example 1, the difference of Comparative Example 4 is that the low-temperature plasma treatment in step S4.5 is removed in Comparative Example 4, and the remaining steps remain unchanged to prepare the collagen peptide composition, which is recorded as Comparative Example 4.

[0044] Comparative Example 5 Compared with Example 1, the difference of Comparative Example 4 is that, in Comparative Example 5, step S4.3 is removed, and S4.4 is replaced by “mixing 2 parts by weight of Haematococcus pluvialis emulsion and 2 parts by weight of rosemary essential oil emulsion with 1 part by weight of wall material solution, and then adjusting the pH to 11 with sodium hydroxide solution, ultrasonically treating at 200 W, 10 kHz for 3 min, and then adjusting to neutrality with hydrogen chloride, and stirring and mixing at 5000 r / min for 30 min to obtain a mixed solution, and replacing the mixed solution I with the mixed solution II in step S4.5 with the mixed solution, and the remaining steps remain unchanged to prepare a collagen peptide composition, which is recorded as Comparative Example 5.

[0045] The data of Example 1 were used continuously, and the collagen peptide compositions prepared in Comparative Examples 1-5 were tested for DPPH clearance rate, anti-fracture strength and scalp health. The test results are shown in Table 2.

[0046] Table 2. Comparative Examples 1-5 DPPH·scavenging rate, anti-breaking strength and scalp health test results

[0047] It can be seen from the data in Table 2 that the data of Comparative Example 1 is lower than that of Example 1, indicating that after simply physically mixing and adding gallic acid, sodium hyaluronate and collagen peptide, the effect on hair growth and toughness and reducing hair aging is not as good as the effect of grafting collagen peptide after sodium hyaluronate is modified by gallic acid, indicating that the grafting of fish bone collagen peptide by gallic acid modified sodium hyaluronate is separated or degraded during use through chemical bond bonding, which effectively improves the bioavailability, so the effect after modification and grafting is better, which is more conducive to promoting hair growth and toughness, reducing hair aging, and avoiding hair loss and white hair; similarly, the data of Comparative Example 2 is lower than that of Example 1, indicating that the use of pea seedlings and pear cactus to assist in the bidirectional fermentation of Dendrobium officinale and Schizophyllum can increase the antioxidant properties of the fermentation liquid, further improve the product to promote hair growth and toughness, and reduce hair aging; from the data of Comparative Examples 3-5, it can be seen that the antioxidant effect of Haematococcus pluvialis and rosemary essential oil can be promoted by dual-core encapsulation after ultrasonic treatment combined with low-temperature plasma treatment.

[0048] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A method for preparing an anti-aging and hair-toughening collagen peptide composition, characterized in that: The steps include: S1: Pretreatment of raw materials, Silver carp skin is subjected to impurity removal, degreasing and decalcification treatment; S2: Extraction and preparation of collagen peptides, The collagen peptides were extracted by ultrasound combined with multi-step acid enzymatic hydrolysis; S3: Preparation of gallic acid-sodium hyaluronate grafted collagen peptide, Sodium hyaluronate is modified with gallic acid and then grafted onto collagen peptide to prepare gallic acid-sodium hyaluronate grafted collagen peptide; S4: Preparation of Haematococcus pluvialis composite microcapsules, Soy lecithin and soy protein isolate were used as wall materials to encapsulate Haematococcus pluvialis and rosemary essential oil emulsion, and then they were encapsulated with gum arabic and treated with low-temperature plasma to prepare Haematococcus pluvialis composite microcapsules. S5: Preparation of pea seedling and prickly pear cactus assisted bidirectional fermentation, The bidirectional fermentation of Schizophyllum and Dendrobium officinale was assisted by pea seedlings and Opuntia pomegranate. S6: Preparation of collagen peptide composition, 3-5 parts by weight of gallic acid-sodium hyaluronate grafted collagen peptide, 2-3 parts by weight of Haematococcus pluvialis composite microcapsules, 6-8 parts by weight of fermentation liquid, 1-2 parts by weight of wheat germ powder and 1-2 parts by weight of ginseng powder are mixed to obtain a collagen peptide composition.

2. The method for preparing an anti-aging and hair-toughening collagen peptide composition according to claim 1, characterized in that: Step S1: Pretreatment of raw materials, specifically including the following steps: S1.1: silver carp skin is recovered from the waste materials of the surimi factory, the skin is cut into pieces, the cut pieces are placed in a vacuum freeze dryer and dried for 48-50 hours, the freeze-dried pieces are placed in a sealed bag, and placed in a dryer for later use; S1.2: The chopped fish skin is homogenized and stirred in a 0.1-0.2 mol / L sodium hydroxide solution for 5-10 min, then soaked for 6-7 h, the alkali solution is replaced every 2-3 h, and finally washed to neutrality and filtered to obtain a fish skin mixture after removing impurities such as protein and pigment; S1.3: Soak 1-2 parts by weight of the fish skin mixture in 10-12 parts by weight of a 10-12wt% isopropanol solution for 9-12 hours, replace the solution every 3-4 hours, then rinse and filter with distilled water for 2-3 times, then put it into 10-12 parts by weight of a 0.3mol / L EDTA solution and soak it for 9-12 hours, then rinse and filter with distilled water for 2-3 times, and centrifuge to obtain the pretreated raw material.

3. The method for preparing an anti-aging and hair-toughening collagen peptide composition according to claim 2, characterized in that: Step S2: Extraction and preparation of collagen peptides, specifically comprising the following steps: S2.1: Soak 3-5 parts by weight of the pretreated raw material in 15-20 parts by weight of 0.5-0.8 mol / L lactic acid solution, homogenize in an ice bath for 5-8 minutes, ultrasonicate at 4-5°C, frequency 60-70 kHz, time 15-20 minutes, power 100-200 W, add pepsin at 20-22 U / g, and hydrolyze for 10-12 hours to obtain the first hydrolyzate; S2.2: The first hydrolyzate is ultrasonicated at 30-40°C, frequency 60-70kHz, time 15-20min, power 100-200W, 10-12U / g papain is added, and the hydrolysis is carried out for 10-12h, and then 10-12U / g bromelain is added, and the hydrolysis is carried out for 10-12h to obtain the hydrolyzate; S2.3: The enzymatic hydrolysis product is heated to 90-95°C for inactivation and maintained for 20-30 minutes, then cooled to 35-40°C and filtered. The filtrate is ultrafiltered using a hollow fiber membrane with a molecular weight cutoff of 10,000 Da, and the permeate is freeze-dried to obtain collagen peptides.

4. The method for preparing an anti-aging and hair-toughening collagen peptide composition according to claim 3, characterized in that: Step S3: Preparation of gallic acid-sodium hyaluronate grafted collagen peptide, specifically comprising the following steps: S3.1: Add 2-3 parts by weight of sodium hyaluronate to 30-50 parts by weight of 2-3% formic acid solution, stir and mix for 10-12 minutes, then add 0.1-0.2 parts by weight of Vc and 1-2 parts by weight of gallic acid, stir and react for 20-30 minutes, then add 8-10 parts by weight of hydrogen peroxide, stir and mix for 20-24 hours, and then perform ultrafiltration with a hollow fiber membrane with a molecular weight cutoff of 10000Da, and freeze-dry the filtrate to obtain gallic acid-sodium hyaluronate; S3.2: Dissolve 2-3 parts by weight of gallic acid-sodium hyaluronate in 30-50 parts by weight of deionized water, then add 5-8 parts by weight of collagen peptide, stir and mix, then add 0.2-0.5 parts by weight of transglutaminase, react for 5-6 hours, then raise the temperature to 30-50°C, react for 15-20 minutes, cool after the reaction is completed, ultrafilter with a hollow fiber membrane with a molecular weight cutoff of 10,000Da, and freeze-dry the filtrate to obtain gallic acid-sodium hyaluronate grafted collagen peptide.

5. The method for preparing an anti-aging and hair-toughening collagen peptide composition according to claim 4, characterized in that: Step S4 is the preparation of Haematococcus pluvialis composite microcapsules, which specifically comprises the following steps: S4.1: dissolving 1-2 parts by weight of soy lecithin and 1-2 parts by weight of soy protein isolate in 50-60 parts by weight of deionized water at 40-50° C. to obtain a wall material solution; S4.2: 1-2 parts by weight of Haematococcus pluvialis, 0.5-0.8 parts by weight of Tween 80 and 30-50 parts by weight of deionized water are mixed and sheared and stirred at 8000-9000 r / min for 10-12 min to obtain a Haematococcus pluvialis emulsion; 1-2 parts by weight of rosemary essential oil, 0.5-0.8 parts by weight of Tween 80 and 30-50 parts by weight of deionized water are mixed and sheared and stirred at 8000-9000 r / min for 10-12 min to obtain a rosemary essential oil emulsion; S4.3: 2-3 parts by weight of Haematococcus pluvialis emulsion and 1-2 parts by weight of wall material solution are mixed, and then the pH is adjusted to 11-12 with a sodium hydroxide solution, and after ultrasonic treatment, the pH is adjusted to neutral with hydrogen chloride, and the mixture is stirred at 5000-6000 r / min for 30-40 min to obtain a mixed solution I; S4.4: 2-3 parts by weight of rosemary essential oil emulsion and 1-2 parts by weight of wall material solution are mixed, and then the pH is adjusted to 11-12 with sodium hydroxide solution, and after ultrasonic treatment, the pH is adjusted to neutral with hydrogen chloride, and the mixture is stirred at 5000-6000 r / min for 30-40 min to obtain a mixed solution II; S4.5: Mix the mixed solution I and the mixed solution II, add 2-3 parts by weight of 1-2wt% gum arabic solution, and then stir and mix at 200-300r / min for 20-30min, transfer the mixed solution into a quartz reactor with 1-1.2A, 50-60W low-temperature plasma for 60-70s treatment, spray dry the treated mixed solution to obtain Haematococcus pluvialis composite microcapsules.

6. The method for preparing an anti-aging and hair-toughening collagen peptide composition according to claim 3, characterized in that: Step S5: preparation of bean sprouts and prickly pear cactus assisted bidirectional fermentation, specifically comprising the following steps: S5.1: Dry the Dendrobium officinale, pea seedlings, and Opuntia pyri in a constant temperature oven at 50-60°C to constant weight, grind them with a traditional Chinese medicine grinder, and pass them through a 30-40 mesh sieve to obtain a mixed powder, seal the mixed powder in a ziplock bag, and store it in a dry state; S5.2: 3-5 g of the mixed powder, 2-3 g of yeast extract, 0.5-0.8 g of magnesium sulfate, 1-2 g of potassium dihydrogen phosphate, 30-50 g of glucose, and 1-1.2 L of deionized water are mixed to obtain a culture medium, and the culture medium is adjusted to 6-6.5 to obtain a culture medium; S5.3: After activating the Schizophyllum, culture it and inoculate the obtained Schizophyllum seed liquid into the culture medium, and then ferment it at 25-26°C and 150-160r / min for 40-48h. Centrifuge the fermentation product to obtain the fermentation liquid.

7. The method for preparing an anti-aging and hair-toughening collagen peptide composition according to claim 4, characterized in that: Step S6: Preparation of the collagen peptide composition, specifically comprising the following steps: S6.1: adding 3-5 parts by weight of gallic acid-sodium hyaluronate grafted collagen peptide and 2-3 parts by weight of Haematococcus pluvialis composite microcapsules to 6-8 parts by weight of fermentation broth, stirring and mixing to obtain a mixture; S6.2: Add 1-2 parts by weight of wheat germ powder and 1-2 parts by weight of ginseng powder to the mixture, and stir and mix at 200-300 r / min for 20-30 min to obtain a collagen peptide composition.

8. The method for preparing an anti-aging and hair-toughening collagen peptide composition according to claim 2, characterized in that: In step S1.2, the ratio of fish skin to sodium hydroxide solution is 1:25-27 w / v.

9. The method for preparing an anti-aging and hair-toughening collagen peptide composition according to claim 5, characterized in that: The parameters of the ultrasonic treatment in steps S4.3 and S4.4 are 200-230 W, 10-12 kHz, and 3-5 min.

10. An anti-aging and hair-toughening collagen peptide composition, characterized in that: The hair-strengthening collagen peptide composition is prepared by the method for preparing the anti-aging and hair-strengthening collagen peptide composition according to any one of claims 1 to 9.

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