Bio-enzyme phosphorus removal agent and preparation method thereof

Abstract

The invention discloses a biological enzyme phosphorus removal agent and a preparation method thereof, and relates to the technical field of biological enzyme phosphorus removal agents. The preparation method specifically comprises the following steps: S1, dissolving cellulose, polycaprolactone, aluminum oxide and polypropylene microspheres into 500ml of water according to a formula ratio of 2: 2: 1: 3, mixing and stirring, and adding 15-32ml of triethylene tetramine for cross-linking reaction to prepare a cured carrier; s2, uniformly mixing the bio-enzyme and the auxiliary agent according to a formula ratio of 3: 1, adding the mixture into the carrier by utilizing a nanotechnology, and fully mixing and stirring to obtain a semi-finished product of a mixed solution of the bio-enzyme phosphorus removal agent; s3, propylene glycol, sodium sorbate, polyvinylpyrrolidone and phosphate are added into the mixed solution according to the formula proportion of 1: 1.2: 0.8: 1 in the mode of adding while stirring, and the stirring speed is controlled to range from 150 r / min to 200 r / min. Phosphorus compounds in wastewater are effectively degraded, and the content of phosphorus pollutants in water is reduced, so that the quality of the water is improved.

Description

Technical Field

[0001] The invention relates to the technical field of bio-enzyme dephosphorization agents, and in particular to a bio-enzyme dephosphorization agent and a preparation method thereof. Background Art

[0002] Bioenzyme phosphorus remover is a product that uses the action of bioenzymes to degrade phosphorus in water. Phosphorus is an important nutrient element, but excessive phosphorus can lead to eutrophication of water bodies, cause algae to reproduce in large numbers, cause water quality deterioration, and even cause environmental problems such as red tides.

[0003] The main factors that cause algae to grow in large numbers are nitrogen and phosphorus. Phosphorus is an important factor that causes eutrophication of water bodies. In water bodies polluted by phosphorus, algae grow in large numbers. After the algae die and decompose, they will produce musty and smelly water bodies. Many species will also produce toxins and affect human health through the food chain. Therefore, it is of great significance to reduce the phosphorus content in sewage.

[0004] Currently, commercial chemical phosphorus removal agents on the market are mainly aluminum ion phosphorus removal agents, such as polyaluminum chloride. However, the phosphorus removal effect of aluminum ion phosphorus removal agents is poor. When using them to remove phosphorus from sewage, a large amount of agents often need to be added, which is costly. The treated wastewater that meets the standards often contains a certain amount of aluminum ions, which will cause a certain degree of adverse effects on the human body or aquatic organisms. Summary of the invention

[0005] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a bioenzyme dephosphorus agent and a preparation method thereof.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: A biological enzyme dephosphorization agent, comprising biological enzymes, auxiliary agents, carriers and additives, and its mass ratio is as follows: Biological enzyme 21%-52%; Auxiliary agents 12%-35%; Carrier 13%-40%; Additives 4%-10%.

[0007] Preferably: the biological enzymes include phosphatase, nuclease, aminophosphatase, glucuronidase, lipase, protease and esterase; The auxiliary agents include surfactants, buffers and nutrients, wherein the surfactants include fatty alcohol polyoxyethylene ether, sodium alkylbenzene sulfonate and octyl polyoxyethylene ether, and the buffers include sodium dihydrogen phosphate and ethylenediaminetetraacetic acid.

[0008] Furthermore: the nutrients include glucose, nitrates, trace elements and vitamin C.

[0009] Based on the above scheme: the trace elements are selected from any two or more of iron, zinc, magnesium and manganese.

[0010] A more preferred embodiment of the aforementioned embodiment is that the carrier comprises cellulose, polycaprolactone, alumina and polypropylene microspheres.

[0011] As a further embodiment of the present invention: the additives include propylene glycol, sodium sorbate, polyvinyl pyrrolidone and phosphate.

[0012] Meanwhile, the biological enzyme, auxiliary agent, carrier and additive have the following specific mass components: 7-10 parts of phosphatase, 6-15 parts of nuclease, 9-17 parts of aminophosphatase, 8-14 parts of glucuronidase, 6-9 parts of lipase, 5-8 parts of protease, 4-7 parts of esterase, 8-12 parts of fatty alcohol polyoxyethylene ether, 7-11 parts of sodium alkylbenzene sulfonate, 1-5 parts of octyl polyoxyethylene ether, 5-8 parts of sodium dihydrogen phosphate, 8-11 parts of ethylenediaminetetraacetic acid, 1-6 parts of glucose, 2-5 parts of nitrate, 1-3 parts of zinc, 1-3 parts of magnesium, 2-8 parts of vitamin C, 10-14 parts of cellulose, 9-16 parts of polycaprolactone, 7-12 parts of alumina, 2-7 parts of polypropylene microspheres, 2-9 parts of propylene glycol, 4-11 parts of sodium sorbate, 3-6 parts of polyvinyl pyrrolidone, and 4-8 parts of phosphate.

[0013] A method for preparing a bio-enzyme dephosphorizing agent comprises the following steps: S1: dissolving cellulose, polycaprolactone, alumina and polypropylene microspheres in 500 ml of water in a ratio of 2:2:1:3, mixing and stirring, and adding 15-32 ml of triethylenetetramine for cross-linking reaction to obtain a solidified carrier; S2: The biological enzyme and the auxiliary agent are mixed evenly in a formula ratio of 3:1, added into the carrier by using nanotechnology, and fully mixed and stirred to obtain a mixed solution of a semi-finished biological enzyme dephosphorization agent; S3: adding propylene glycol, sodium sorbate, polyvinyl pyrrolidone and phosphate into the mixed solution in a ratio of 1:1.2:0.8:1 while stirring, and controlling the stirring speed to 150-200 r / min; S4: adding 10-20 ml of sodium acetate to the mixed solution to adjust the pH value of the mixed solution; S5: placing the mixed solution of the semi-finished bio-enzyme dephosphorus agent in a sterilization cabinet for sterilization, and drying the mixed solution to obtain a solid bio-enzyme dephosphorus agent.

[0014] Meanwhile, in the step S2, the biological enzyme and the auxiliary agent are mixed evenly according to the formula ratio, and added into the carrier by using nanotechnology, and the specific steps are as follows: S21: preparing the retained solid carrier into a carrier with a nanostructured form; S22: mixing the biological enzyme and the auxiliary agent in a ratio of 3:1 to form a solution; S23: mixing and stirring the nanocarrier with the biological enzyme and auxiliary agent solution, and making use of the high specific surface area and adsorption properties of the nanocarrier to allow the biological enzyme and auxiliary agent to be adsorbed onto the surface of the nanocarrier, the mixing and stirring speed is 200-800 r / min, the stirring time is 1-2 h, and the stirring temperature is 12-25° C.; S24: cooling and standing naturally for 20-25 minutes to allow the biological enzyme and auxiliary agent to be evenly distributed on the nanocarrier; S25: drying and cross-linking the nanocarriers loaded with the biological enzyme and the auxiliary agent.

[0015] As a more preferred solution of the present invention: the nanocarrier is prepared in step S21, and the specific steps are as follows: S211: dissolving the prepared solid carrier into water to form a uniform carrier solution; S212: uniformly coating the carrier solution on a flat substrate to form a thin film coating; S213: placing the substrate coated with the carrier solution in a sterile environment, and controlling the ambient temperature at 29-48°C, the humidity at 30-52%rh, and the ventilation volume at 11-32L / min; S214: observing changes on the substrate surface, and controlling the morphology and size of the nanostructure formed by the carrier by adjusting the temperature, humidity and ventilation volume; S215: After the solvent is completely evaporated, the nano-shaped carrier is collected by peeling.

[0016] The beneficial effects of the present invention are: 1. A bio-enzyme phosphorus removal agent and a preparation method thereof. Enzymes such as phosphatase and aminophosphatase can effectively degrade phosphorus compounds in wastewater, help reduce the content of phosphorus pollutants in water bodies, and thus improve water quality. At the same time, enzymes such as glucuronidase, lipase, and protease can accelerate the degradation process of organic matter, improve the treatment efficiency of wastewater, and make the treatment process more efficient. In addition to enzyme components, it also contains a variety of other ingredients, such as fatty alcohol polyoxyethylene ether, sodium alkylbenzene sulfonate, vitamin C, etc., which enables the bio-enzyme phosphorus removal agent to comprehensively treat different types of wastewater, and is suitable for various wastewater treatment scenarios such as urban sewage treatment plants and industrial wastewater treatment. Through the synergistic effect of multiple enzymes and other components, it can efficiently remove phosphorus and degrade organic matter. It has the advantages of environmental protection, pollution-free, multifunctional, safe and reliable, and has a wide range of applications.

[0017] 2. This kind of bio-enzyme dephosphorization agent and its preparation method can improve the utilization rate and activity of the bio-enzyme and increase the wastewater treatment efficiency by evenly mixing the bio-enzyme and the auxiliary agent and adsorbing them onto the surface of the nano-carrier. The bio-enzyme and the auxiliary agent are fixed on the nano-carrier, which can increase their stability, extend their service life, and reduce the activity loss caused by changes in external conditions. At the same time, through the process of mixing, stirring and standing, the bio-enzyme and the auxiliary agent are evenly distributed on the nano-carrier, ensuring the uniformity and stability of the product. Compared with traditional chemical methods, the use of bio-enzymes and auxiliary agents to treat wastewater is more environmentally friendly. The use of nanotechnology to prepare nano-carriers helps to reduce the impact on the environment during the wastewater treatment process. The nano-carriers have a high specific surface area and adsorption properties, can better adsorb the bio-enzymes and auxiliary agents, and improve the adsorption capacity and effect of the product.

[0018] 3. A bio-enzyme dephosphorizer and a preparation method thereof, wherein the solid carrier is dissolved in water and coated on a substrate, and the temperature, humidity and ventilation volume are controlled to prepare the carrier in a sterile environment, which is conducive to forming a carrier with a nanostructure, and increases the specific surface area and adsorption performance of the carrier. The solid carrier is dissolved and evenly coated on the substrate, which is conducive to forming a uniform carrier film coating, and the uniformity and stability of the carrier are ensured. The morphology and size of the nanostructure formed by the carrier can be accurately adjusted by controlling the ambient temperature, humidity and ventilation volume, and accurate control of the carrier structure is achieved. In addition, preparation in a sterile environment is conducive to reducing the impact of external pollution on the carrier preparation, and the purity and quality stability of the product are ensured. The nano-shaped carrier is collected by stripping after the solvent is completely volatilized, which is convenient for subsequent processing and utilization, and improves the collection efficiency and product utilization rate. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0021] A biological enzyme dephosphorization agent, comprising biological enzymes, auxiliary agents, carriers and additives, and its mass ratio is as follows: Biological enzyme 21%-52%; Auxiliary agents 12%-35%; Carrier 13%-40%; Additives 4%-10%.

[0022] The biological enzyme, adjuvant, carrier and additive have the following specific ingredients: Biological enzymes include phosphatases, nucleases, aminophosphatases, glucuronidases, lipases, proteases, and esterases; The auxiliary agents include surfactants, buffers and nutrients, wherein the surfactants include fatty alcohol polyoxyethylene ether, sodium alkylbenzene sulfonate and octyl polyoxyethylene ether, the buffers include sodium dihydrogen phosphate and ethylenediaminetetraacetic acid, and the nutrients include glucose, nitrates, trace elements and vitamin C, wherein the trace elements are selected from any two or more of iron, zinc, magnesium and manganese.

[0023] Supports include cellulose, polycaprolactone, alumina, and polypropylene microspheres; Additives include propylene glycol, sodium sorbate, polyvinyl pyrrolidone and phosphates.

[0024] Enzymes such as phosphatase and aminophosphatase can effectively degrade phosphorus compounds in wastewater, help reduce the content of phosphorus pollutants in water bodies, and thus improve water quality. At the same time, enzymes such as glucuronidase, lipase, and protease can accelerate the degradation process of organic matter, improve the efficiency of wastewater treatment, and make the treatment process more efficient. In addition to enzyme components, it also contains a variety of other ingredients, such as fatty alcohol polyoxyethylene ether, sodium alkylbenzene sulfonate, vitamin C, etc., which allows bio-enzyme phosphorus removal agents to comprehensively treat different types of wastewater, and are suitable for various wastewater treatment scenarios such as urban sewage treatment plants and industrial wastewater treatment. Through the synergistic effect of multiple enzymes and other ingredients, it can efficiently remove phosphorus and degrade organic matter. It has the advantages of environmental protection, pollution-free, multifunctional, safe and reliable, and has a wide range of applications.

[0025] The biological enzyme, adjuvant, carrier and additive have the following specific mass components: 7-10 parts of phosphatase, 6-15 parts of nuclease, 9-17 parts of aminophosphatase, 8-14 parts of glucuronidase, 6-9 parts of lipase, 5-8 parts of protease, 4-7 parts of esterase, 8-12 parts of fatty alcohol polyoxyethylene ether, 7-11 parts of sodium alkylbenzene sulfonate, 1-5 parts of octyl polyoxyethylene ether, 5-8 parts of sodium dihydrogen phosphate, 8-11 parts of ethylenediaminetetraacetic acid, 1-6 parts of glucose, 2-5 parts of nitrate, 1-3 parts of zinc, 1-3 parts of magnesium, 2-8 parts of vitamin C, 10-14 parts of cellulose, 9-16 parts of polycaprolactone, 7-12 parts of alumina, 2-7 parts of polypropylene microspheres, 2-9 parts of propylene glycol, 4-11 parts of sodium sorbate, 3-6 parts of polyvinyl pyrrolidone, and 4-8 parts of phosphate.

[0026] The bio-enzyme dephosphorizing agent involved in the following examples is prepared by the following preparation method: S1: dissolving cellulose, polycaprolactone, alumina and polypropylene microspheres in 500 ml of water in a ratio of 2:2:1:3, mixing and stirring, and adding 15-32 ml of triethylenetetramine for cross-linking reaction to obtain a solidified carrier; S2: The biological enzyme and the auxiliary agent are mixed evenly in a formula ratio of 3:1, added into the carrier by using nanotechnology, and fully mixed and stirred to obtain a mixed solution of a semi-finished biological enzyme dephosphorization agent; S3: adding propylene glycol, sodium sorbate, polyvinyl pyrrolidone and phosphate into the mixed solution in a ratio of 1:1.2:0.8:1 while stirring, and controlling the stirring speed to 150-200 r / min; S4: adding 10-20 ml of sodium acetate to the mixed solution to adjust the pH value of the mixed solution; S5: placing the mixed solution of the semi-finished bio-enzyme dephosphorus agent in a sterilization cabinet for sterilization, and drying the mixed solution to obtain a solid bio-enzyme dephosphorus agent.

[0027] In the step S2, the biological enzyme and the auxiliary agent are mixed evenly according to the formula ratio, and added into the carrier by using nanotechnology, and the specific steps are as follows: S21: preparing the retained solid carrier into a carrier with a nanostructured form; S22: mixing the biological enzyme and the auxiliary agent in a ratio of 3:1 to form a solution; S23: mixing and stirring the nanocarrier with the biological enzyme and auxiliary agent solution, and making use of the high specific surface area and adsorption properties of the nanocarrier to allow the biological enzyme and auxiliary agent to be adsorbed onto the surface of the nanocarrier, the mixing and stirring speed is 200-800 r / min, the stirring time is 1-2 h, and the stirring temperature is 12-25° C.; S24: cooling and standing naturally for 20-25 minutes to allow the biological enzyme and auxiliary agent to be evenly distributed on the nanocarrier; S25: drying and cross-linking the nanocarriers loaded with the biological enzyme and the auxiliary agent.

[0028] By evenly mixing the biological enzyme and the auxiliary agent and adsorbing them onto the surface of the nanocarrier, the utilization rate and activity of the biological enzyme can be improved, and the wastewater treatment efficiency can be increased. By fixing the biological enzyme and the auxiliary agent on the nanocarrier, their stability can be increased, their service life can be extended, and the activity loss caused by changes in external conditions can be reduced. At the same time, through the process of mixing, stirring and standing, the biological enzyme and the auxiliary agent are evenly distributed on the nanocarrier, ensuring the uniformity and stability of the product. Using biological enzymes and auxiliary agents to treat wastewater is more environmentally friendly than traditional chemical methods. The use of nanotechnology to prepare nanocarriers helps to reduce the impact on the environment during wastewater treatment. The nanocarrier has a high specific surface area and adsorption properties, which can better adsorb biological enzymes and auxiliary agents, thereby improving the adsorption capacity and effect of the product.

[0029] Wherein, the nanocarrier is prepared in step S21, and the specific steps are as follows: S211: dissolving the prepared solid carrier into water to form a uniform carrier solution; S212: uniformly coating the carrier solution on a flat substrate to form a thin film coating; S213: placing the substrate coated with the carrier solution in a sterile environment, and controlling the ambient temperature at 29-48°C, the humidity at 30-52%rh, and the ventilation volume at 11-32L / min; S214: observing changes on the substrate surface, and controlling the morphology and size of the nanostructure formed by the carrier by adjusting the temperature, humidity and ventilation volume; S215: After the solvent is completely evaporated, the nano-shaped carrier is collected by peeling.

[0030] By dissolving the solid carrier in water and coating it on a substrate, controlling the temperature, humidity and ventilation volume, and preparing the carrier in a sterile environment, it is beneficial to form a nanostructured carrier, which increases the specific surface area and adsorption performance of the carrier. Dissolving the solid carrier and evenly coating it on the substrate is beneficial to forming a uniform carrier film coating, ensuring the uniformity and stability of the carrier. By controlling the ambient temperature, humidity and ventilation volume, the morphology and size of the nanostructure formed by the carrier can be accurately adjusted to achieve precise control of the carrier structure. In addition, preparation in a sterile environment is beneficial to reducing the impact of external pollution on carrier preparation, ensuring the purity and quality stability of the product. The nano-shaped carrier is collected by stripping after the solvent is completely volatilized, which is convenient for subsequent processing and utilization, thereby improving the collection efficiency and product utilization rate.

[0031] Embodiment 1: A biological enzyme dephosphorization agent, wherein the biological enzyme, auxiliary agent, carrier and additive have the following specific mass components: Phosphatase 7 parts, nuclease 6 parts, aminophosphatase 9 parts, glucuronidase 8 parts, lipase 6 parts, protease 5 parts, esterase 4 parts, fatty alcohol polyoxyethylene ether 8 parts, sodium alkylbenzene sulfonate 7 parts, octyl polyoxyethylene ether 1 part, sodium dihydrogen phosphate 5 parts, ethylenediaminetetraacetic acid 8 parts, glucose 1 part, nitrate 2 parts, zinc 1 part, magnesium 1 part, vitamin C 2 parts, cellulose 10 parts, polycaprolactone 9 parts, alumina 7 parts, polypropylene microspheres 2 parts, propylene glycol 2 parts, sodium sorbate 4 parts, polyvinyl pyrrolidone 3 parts, phosphate 4 parts.

[0032] A method for preparing a bio-enzyme dephosphorizing agent comprises the following steps: S1: Dissolve cellulose, polycaprolactone, aluminum oxide and polypropylene microspheres in 500 ml of water in a ratio of 2:2:1:3, mix and stir, and add 18 ml of triethylenetetramine for cross-linking reaction to prepare a solidified carrier; S2: The biological enzyme and the auxiliary agent are mixed evenly in a formula ratio of 3:1, added into the carrier by using nanotechnology, and fully mixed and stirred to obtain a mixed solution of a semi-finished biological enzyme dephosphorization agent; S3: Propylene glycol, sodium sorbate, polyvinyl pyrrolidone and phosphate are added to the mixed solution in a ratio of 1:1.2:0.8:1 while stirring, and the stirring speed is controlled at 165 r / min; S4: adding 15 ml of sodium acetate to the mixed solution to adjust the pH value of the mixed solution; S5: placing the mixed solution of the semi-finished bio-enzyme dephosphorus agent in a sterilization cabinet for sterilization, and drying the mixed solution to obtain a solid bio-enzyme dephosphorus agent.

[0033] In the step S2, the biological enzyme and the auxiliary agent are mixed evenly according to the formula ratio, and added into the carrier by using nanotechnology, and the specific steps are as follows: S21: preparing the retained solid carrier into a carrier with a nanostructured form; S22: mixing the biological enzyme and the auxiliary agent in a ratio of 3:1 to form a solution; S23: mixing and stirring the nanocarrier with the biological enzyme and auxiliary agent solution, and making use of the high specific surface area and adsorption properties of the nanocarrier to allow the biological enzyme and auxiliary agent to be adsorbed onto the surface of the nanocarrier, with a mixing and stirring speed of 425 r / min, a stirring time of 1.3 h, and a stirring temperature of 18° C.; S24: cooling and standing naturally for 20 minutes to allow the biological enzyme and auxiliary agent to be evenly distributed on the nanocarrier; S25: drying and cross-linking the nanocarriers loaded with the biological enzyme and the auxiliary agent.

[0034] Wherein, the nanocarrier is prepared in step S21, and the specific steps are as follows: S211: dissolving the prepared solid carrier into water to form a uniform carrier solution; S212: uniformly coating the carrier solution on a flat substrate to form a thin film coating; S213: placing the substrate coated with the carrier solution in a sterile environment, and controlling the ambient temperature at 34°C, the humidity at 34% rh, and the ventilation volume at 17 L / min; S214: observing changes on the substrate surface, and controlling the morphology and size of the nanostructure formed by the carrier by adjusting the temperature, humidity and ventilation volume; S215: After the solvent is completely evaporated, the nano-shaped carrier is collected by peeling.

[0035] Embodiment 2: A biological enzyme dephosphorization agent, wherein the biological enzyme, auxiliary agent, carrier and additive have the following specific mass components: 8 parts of phosphatase, 10 parts of nuclease, 9 parts of aminophosphatase, 10 parts of glucuronidase, 7 parts of lipase, 6 parts of protease, 5 parts of esterase, 9 parts of fatty alcohol polyoxyethylene ether, 8 parts of sodium alkylbenzene sulfonate, 3 parts of octyl polyoxyethylene ether, 6 parts of sodium dihydrogen phosphate, 8 parts of ethylenediaminetetraacetic acid, 4 parts of glucose, 3 parts of nitrate, 1 part of zinc, 2 parts of magnesium, 5 parts of vitamin C, 12 parts of cellulose, 10 parts of polycaprolactone, 8 parts of alumina, 5 parts of polypropylene microspheres, 3 parts of propylene glycol, 6 parts of sodium sorbate, 5 parts of polyvinyl pyrrolidone, and 7 parts of phosphate.

[0036] A method for preparing a bio-enzyme dephosphorizing agent comprises the following steps: S1: Dissolve cellulose, polycaprolactone, aluminum oxide and polypropylene microspheres in 500 ml of water in a ratio of 2:2:1:3, mix and stir, and add 20 ml of triethylenetetramine for cross-linking reaction to prepare a solidified carrier; S2: The biological enzyme and the auxiliary agent are mixed evenly in a formula ratio of 3:1, added into the carrier by using nanotechnology, and fully mixed and stirred to obtain a mixed solution of a semi-finished biological enzyme dephosphorization agent; S3: Propylene glycol, sodium sorbate, polyvinyl pyrrolidone and phosphate are added to the mixed solution in a ratio of 1:1.2:0.8:1 while stirring, and the stirring speed is controlled at 180 r / min; S4: adding 15 ml of sodium acetate to the mixed solution to adjust the pH value of the mixed solution; S5: placing the mixed solution of the semi-finished bio-enzyme dephosphorus agent in a sterilization cabinet for sterilization, and drying the mixed solution to obtain a solid bio-enzyme dephosphorus agent.

[0037] In the step S2, the biological enzyme and the auxiliary agent are mixed evenly according to the formula ratio, and added into the carrier by using nanotechnology, and the specific steps are as follows: S21: preparing the retained solid carrier into a carrier with a nanostructured form; S22: mixing the biological enzyme and the auxiliary agent in a ratio of 3:1 to form a solution; S23: mixing and stirring the nanocarrier with the biological enzyme and auxiliary agent solution, and making use of the high specific surface area and adsorption properties of the nanocarrier to allow the biological enzyme and auxiliary agent to be adsorbed onto the surface of the nanocarrier, with a mixing and stirring speed of 512 r / min, a stirring time of 1 h, and a stirring temperature of 20° C.; S24: cooling and standing naturally for 23 minutes to allow the biological enzyme and auxiliary agent to be evenly distributed on the nanocarrier; S25: drying and cross-linking the nanocarriers loaded with the biological enzyme and the auxiliary agent.

[0038] Wherein, the nanocarrier is prepared in step S21, and the specific steps are as follows: S211: dissolving the prepared solid carrier into water to form a uniform carrier solution; S212: uniformly coating the carrier solution on a flat substrate to form a thin film coating; S213: placing the substrate coated with the carrier solution in a sterile environment, and controlling the ambient temperature at 41°C, the humidity at 40% rh, and the ventilation volume at 25 L / min; S214: observing changes on the substrate surface, and controlling the morphology and size of the nanostructure formed by the carrier by adjusting the temperature, humidity and ventilation volume; S215: After the solvent is completely evaporated, the nano-shaped carrier is collected by peeling.

[0039] Embodiment 3: A biological enzyme dephosphorization agent, wherein the biological enzyme, auxiliary agent, carrier and additive have the following specific mass components: Phosphatase 7 parts, nuclease 13 parts, aminophosphatase 14 parts, glucuronidase 9 parts, lipase 7 parts, protease 8 parts, esterase 7 parts, fatty alcohol polyoxyethylene ether 10 parts, sodium alkylbenzene sulfonate 9 parts, octyl polyoxyethylene ether 3 parts, sodium dihydrogen phosphate 7 parts, ethylenediaminetetraacetic acid 10 parts, glucose 4 parts, nitrate 5 parts, zinc 3 parts, magnesium 2 parts, vitamin C 6 parts, cellulose 11 parts, polycaprolactone 15 parts, alumina 10 parts, polypropylene microspheres 7 parts, propylene glycol 6 parts, sodium sorbate 7 parts, polyvinyl pyrrolidone 5 parts, phosphate 6 parts.

[0040] A method for preparing a bio-enzyme dephosphorizing agent comprises the following steps: S1: Dissolve cellulose, polycaprolactone, aluminum oxide and polypropylene microspheres in 500 ml of water in a ratio of 2:2:1:3, mix and stir, and add 28 ml of triethylenetetramine for cross-linking reaction to prepare a solidified carrier; S2: The biological enzyme and the auxiliary agent are mixed evenly in a formula ratio of 3:1, added into the carrier by using nanotechnology, and fully mixed and stirred to obtain a mixed solution of a semi-finished biological enzyme dephosphorization agent; S3: Propylene glycol, sodium sorbate, polyvinyl pyrrolidone and phosphate are added to the mixed solution in a ratio of 1:1.2:0.8:1 while stirring, and the stirring speed is controlled at 189 r / min; S4: adding 17 ml of sodium acetate to the mixed solution to adjust the pH value of the mixed solution; S5: placing the mixed solution of the semi-finished bio-enzyme dephosphorus agent in a sterilization cabinet for sterilization, and drying the mixed solution to obtain a solid bio-enzyme dephosphorus agent.

[0041] In the step S2, the biological enzyme and the auxiliary agent are mixed evenly according to the formula ratio, and added into the carrier by using nanotechnology, and the specific steps are as follows: S21: preparing the retained solid carrier into a carrier with a nanostructured form; S22: mixing the biological enzyme and the auxiliary agent in a ratio of 3:1 to form a solution; S23: mixing and stirring the nanocarrier with the biological enzyme and auxiliary agent solution, and making use of the high specific surface area and adsorption properties of the nanocarrier to allow the biological enzyme and auxiliary agent to be adsorbed onto the surface of the nanocarrier, with a mixing and stirring speed of 638 r / min, a stirring time of 2 h, and a stirring temperature of 24° C.; S24: cooling and standing naturally for 25 minutes to allow the biological enzyme and auxiliary agent to be evenly distributed on the nanocarrier; S25: drying and cross-linking the nanocarriers loaded with the biological enzyme and the auxiliary agent.

[0042] Wherein, the nanocarrier is prepared in step S21, and the specific steps are as follows: S211: dissolving the prepared solid carrier into water to form a uniform carrier solution; S212: uniformly coating the carrier solution on a flat substrate to form a thin film coating; S213: placing the substrate coated with the carrier solution in a sterile environment, and controlling the ambient temperature at 45°C, the humidity at 47% rh, and the ventilation volume at 26 L / min; S214: observing changes on the substrate surface, and controlling the morphology and size of the nanostructure formed by the carrier by adjusting the temperature, humidity and ventilation volume; S215: After the solvent is completely evaporated, the nano-shaped carrier is collected by peeling.

[0043] Comparative Example: Take 4 portions of 300 ml of phosphorus-containing sewage respectively, and the phosphorus content is the same. The first three portions are added with the bio-enzyme phosphorus removal agent prepared by the preparation method of this case in Examples 1-3 for mixing and stirring, and the last portion is added with the existing PAC-02 phosphorus removal agent on the market for mixing and stirring. The dosage of the phosphorus removal agent added to the four portions is the same. After treatment, the content of the components (phosphorus content, nucleic acid ester content, amino phosphoric acid ester content, glucose ester content, fat and oil content, protein pollutant content, ester compounds) in the four portions of sewage is detected. The detection means of each substance are as follows: The phosphorus content was measured by spectrophotometer; The content of nucleic acid ester, phosphoramidate and glucose ester was detected by liquid chromatography; The fat and oil content and ester compounds were tested by gas chromatography; The content of protein contaminants was detected by colorimeter; The test results are shown in the following table: According to the above table, the use of the bio-enzyme phosphorus removal agent of the present application can achieve better results than the PAC-02 phosphorus removal agent in the comparative example, with a higher phosphorus removal rate, and the bio-enzyme phosphorus removal agent in Example 3 is the best, reaching 93.3%. Among them, the bio-enzyme phosphorus removal agent in this case also has the ability to remove nucleic acid esters, amino phosphoric acid esters, glucose esters, fats and oils, protein pollutants and ester compounds in sewage, and the removal effect is better than that of the PAC-02 phosphorus removal agent.

[0044] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A bio-enzyme dephosphorization agent, comprising a bio-enzyme, an adjuvant, a carrier and an additive, It is characterized in that The quality ratio is as follows: Biological enzyme 21%-52%; Auxiliary agents 12%-35%; Carrier 13%-40%; Additives 4%-10%.

2. A bio-enzyme dephosphorization agent according to claim 1, It is characterized in that The biological enzymes include phosphatase, nuclease, aminophosphatase, glucuronidase, lipase, protease and esterase; The auxiliary agents include surfactants, buffers and nutrients, wherein the surfactants include fatty alcohol polyoxyethylene ether, sodium alkylbenzene sulfonate and octyl polyoxyethylene ether, and the buffers include sodium dihydrogen phosphate and ethylenediaminetetraacetic acid.

3. A bio-enzyme dephosphorization agent according to claim 2, It is characterized in that The nutrients include glucose, nitrate, trace elements and vitamin C.

4. A bio-enzyme dephosphorization agent according to claim 3, It is characterized in that The trace elements are selected from any two or more of iron, zinc, magnesium and manganese.

5. A bio-enzyme dephosphorization agent according to claim 1, It is characterized in that The carriers include cellulose, polycaprolactone, alumina and polypropylene microspheres.

6. A bio-enzyme dephosphorization agent according to claim 1, It is characterized in that The additives include propylene glycol, sodium sorbate, polyvinyl pyrrolidone and phosphate.

7. A bio-enzyme dephosphorization agent according to claim 1, It is characterized in that The biological enzyme, adjuvant, carrier and additive have the following specific mass components: 7-10 parts of phosphatase, 6-15 parts of nuclease, 9-17 parts of aminophosphatase, 8-14 parts of glucuronidase, 6-9 parts of lipase, 5-8 parts of protease, 4-7 parts of esterase, 8-12 parts of fatty alcohol polyoxyethylene ether, 7-11 parts of sodium alkylbenzene sulfonate, 1-5 parts of octyl polyoxyethylene ether, 5-8 parts of sodium dihydrogen phosphate, 8-11 parts of ethylenediaminetetraacetic acid, 1-6 parts of glucose, 2-5 parts of nitrate, 1-3 parts of zinc, 1-3 parts of magnesium, 2-8 parts of vitamin C, 10-14 parts of cellulose, 9-16 parts of polycaprolactone, 7-12 parts of alumina, 2-7 parts of polypropylene microspheres, 2-9 parts of propylene glycol, 4-11 parts of sodium sorbate, 3-6 parts of polyvinyl pyrrolidone, and 4-8 parts of phosphate.

8. A method for preparing the bio-enzyme phosphorus removal agent according to any one of claims 1 to 7, It is characterized in that The following steps are involved: S1: dissolving cellulose, polycaprolactone, alumina and polypropylene microspheres in 500 ml of water in a ratio of 2:2:1:3, mixing and stirring, and adding 15-32 ml of triethylenetetramine for cross-linking reaction to obtain a solidified carrier; S2: The biological enzyme and the auxiliary agent are mixed evenly in a formula ratio of 3:1, added into the carrier by using nanotechnology, and fully mixed and stirred to obtain a mixed solution of a semi-finished biological enzyme dephosphorization agent; S3: adding propylene glycol, sodium sorbate, polyvinyl pyrrolidone and phosphate into the mixed solution in a ratio of 1:1.2:0.8:1 while stirring, and controlling the stirring speed to 150-200 r / min; S4: adding 10-20 ml of sodium acetate to the mixed solution to adjust the pH value of the mixed solution; S5: placing the mixed solution of the semi-finished bio-enzyme dephosphorus agent in a sterilization cabinet for sterilization, and drying the mixed solution to obtain a solid bio-enzyme dephosphorus agent.

9. The method for preparing a bio-enzyme dephosphorizing agent according to claim 8, It is characterized in that In the step S2, the biological enzyme and the auxiliary agent are mixed evenly according to the formula ratio, and added into the carrier by using nanotechnology, and the specific steps are as follows: S21: preparing the retained solid carrier into a carrier with a nanostructured form; S22: mixing the biological enzyme and the auxiliary agent in a ratio of 3:1 to form a solution; S23: mixing and stirring the nanocarrier with the biological enzyme and auxiliary agent solution, and making use of the high specific surface area and adsorption properties of the nanocarrier to allow the biological enzyme and auxiliary agent to be adsorbed onto the surface of the nanocarrier, the mixing and stirring speed is 200-800 r / min, the stirring time is 1-2 h, and the stirring temperature is 12-25° C.; S24: cooling and standing naturally for 20-25 minutes to allow the biological enzyme and auxiliary agent to be evenly distributed on the nanocarrier; S25: drying and cross-linking the nanocarriers loaded with the biological enzyme and the auxiliary agent.

10. The method for preparing a bio-enzyme dephosphorizing agent according to claim 9, It is characterized in that The nanocarrier is prepared in step S21, and the specific steps are as follows: S211: dissolving the prepared solid carrier into water to form a uniform carrier solution; S212: uniformly coating the carrier solution on a flat substrate to form a thin film coating; S213: placing the substrate coated with the carrier solution in a sterile environment, and controlling the ambient temperature at 29-48°C, the humidity at 30-52%rh, and the ventilation volume at 11-32L / min; S214: observing changes on the substrate surface, and controlling the morphology and size of the nanostructure formed by the carrier by adjusting the temperature, humidity and ventilation volume; S215: After the solvent is completely evaporated, the nano-shaped carrier is collected by peeling.

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