Slide dyeing device for rapid on-site evaluation

Abstract

The invention relates to a slide dyeing device for rapid on-site evaluation. The slide dyeing device comprises a box body, a box body cover, a dyeing assembly and an automatic liquid supply unit, wherein the dyeing assembly and the automatic liquid supply unit are located in the box body; the dyeing assembly comprises a dyeing workbench, a limiting unit, a connecting unit and a driving unit; a through hole allowing the dyeing workbench to extend out is formed in the front face of the box body, when the limiting guide rail is located at the first working position, the dyeing workbench extends out of the through hole, and the two breast board hinges are parallel to each other; when the limiting guide rail is located at the second working position, the two breast board hinges are opened by a set angle, and the five-head dropper is aligned to one end of the dyeing workbench in the vertical direction; when the limiting guide rail is located at the third working position, the dyeing workbench is located below the air drying unit, and the two breast board hinges are parallel to each other. Compared with the prior art, the device is small in size, small in dye consumption and controlled in dyeing time, and the dyeing efficiency and the dyeing quality are improved.

Description

Technical Field

[0001] The invention relates to the technical field of medical clinical testing, and in particular to a slide staining device for rapid on-site evaluation. Background Art

[0002] Rapid on-site evaluation (ROSE) is an extremely fast cytological judgment technology that accompanies diagnostic interventional operations. It can quickly interpret the puncture specimens obtained by the endoscopic ultrasound physician, evaluate whether the adequacy of the specimens can meet the diagnostic needs, provide timely feedback on the spot, and decide whether it is necessary to repeat the puncture operation or change the puncture path to obtain more and higher-quality specimens, thereby improving the diagnosis rate, reducing unnecessary punctures, and thus reducing the occurrence of operation-related complications.

[0003] The most common method for clinical on-site rapid evaluation is the Dif rapid staining method for staining the puncture smear. The reagents used are Dif A solution, Dif B solution and clean water for cleaning. The main staining steps are to soak the prepared slide in Dif A solution for ten to thirty seconds, then soak the slide in Dif B solution for about twenty to forty seconds, rinse with clean water, and after drying, it can be sent to an optical microscope for pathological diagnosis.

[0004] Although existing slide staining components can be used to stain slides in large quantities, they occupy a large space and consume a large amount of dye, making them unsuitable for on-site rapid evaluation during diagnostic interventional procedures. In addition, existing devices mostly use immersion staining technology, which has a high possibility of sample contamination and affects sample quality. At present, it is difficult for most domestic hospitals to achieve automated slide staining in on-site rapid evaluation of diagnostic interventional procedures. Summary of the invention

[0005] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and provide a slide staining device for rapid on-site evaluation. The present invention has a small size, a small amount of dye, a controlled staining time, and improves staining efficiency and staining quality.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] The present invention provides a slide staining device for rapid on-site evaluation, which is a small-volume device that can be placed in an operating room to achieve automated slide staining during endoscopic diagnosis and treatment. The use of the device is conducive to reducing the amount of dye used, controlling the staining time, and improving the staining efficiency and staining quality.

[0008] The present invention provides a slide staining device for rapid on-site evaluation, comprising: a box body, a box body cover, and a staining component and an automatic liquid supply unit located inside the box body;

[0009] The dyeing assembly comprises a dyeing workbench, a limiting unit, a connecting unit and a driving unit; the limiting unit and the driving unit are fixedly connected via the connecting unit, and the dyeing workbench is fixedly connected to the limiting unit;

[0010] The automatic liquid supply unit includes a dye bottle, a clean water tank, a peristaltic pump unit, and a five-head dropper. The peristaltic pump unit includes multiple peristaltic pumps. Each dye bottle is connected to a peristaltic pump, and the clean water tank is connected to a peristaltic pump, and then they are merged into the five-head dropper. When replacing the dye or adding clean water, the box cover is opened to take out the dye bottle and the clean water tank, so that the liquid can be added and replaced.

[0011] The limiting unit includes a slide plate and a limiting guide rail, and the slide plate is provided with a movable latch pin which cooperates with the limiting guide rail; the limiting guide rail has a first working position, a second working position and a third working position: a through hole is provided on the front of the box body for the dyeing workbench to extend out, and when the limiting guide rail is in the first working position, the dyeing workbench extends out from the through hole, and the two railing hinges are parallel to each other; when the limiting guide rail is in the second working position, the two railing hinges are opened to a set angle, and the five-head dropper is vertically aligned with one end of the dyeing workbench; when the limiting guide rail is in the third working position, the dyeing workbench is located below the air-drying unit, and the two railing hinges are parallel to each other.

[0012] Furthermore, the connection unit includes two guardrail hinges, which are rotatably connected and fixedly connected to the limiting unit and the driving unit respectively.

[0013] Furthermore, the driving unit includes: a slider, a lead screw, a slide rail and a stepper motor, the slider is arranged on the slide rail, the slider can slide along the slide rail, and the stepper motor drives the lead screw to achieve linear movement; through the linear movement of the slider, the vertical lifting of the slide plate is achieved, so as to achieve the purpose of increasing the hinge angle of the two fences.

[0014] Furthermore, the staining workbench includes a fast staining table, a glass slide table and two spring sheets. The glass slide table is provided with five glass slide slots and is fixed to the fast staining table by two spring sheets.

[0015] The limiting unit is fixedly connected to the fast dyeing table, so that when the driving unit drives the dyeing table to slide on the slide rail, a certain angle can exist between the dyeing table and the driving unit.

[0016] Furthermore, the rapid dyeing table is provided with a liquid diversion slope, and liquid outlets are provided on both sides of the rapid dyeing table. The outflowing waste liquid is concentrated and collected through the diversion slope and the liquid outlet, which is convenient for subsequent cleaning and treatment.

[0017] Furthermore, when the limiting guide rail is in the first working position, the staining workbench extends from the through hole, and the staining workbench is parallel to the bottom surface of the box body. Five slides to be processed are placed on the slide table in sequence, waiting for staining.

[0018] Further, the peristaltic pump unit includes: a first peristaltic pump, a second peristaltic pump, and a third peristaltic pump;

[0019] When the limit guide rail is in the second working position, the five-head dropper is vertically aligned with one end of the glass stage, and there is an angle between the staining workbench and the bottom surface of the box so that the liquid flows along the slope;

[0020] The first peristaltic pump works to push the dye solution A to the five-head dropper and drop it vertically onto the glass slide. The dyeing workbench is an inclined plane in the second working position, and the dye solution flows through the entire glass slide due to gravity. The stepper motor of the drive unit then drives the dyeing workbench to move to the third working position, where it stands for a period of time to complete the dyeing of the dye solution A. The horizontal third working position can save dye.

[0021] Furthermore, after dyeing with dye A is completed, it returns to the second working position, and the second peristaltic pump starts working to push dye B to the five-head dropper, and drops vertically onto the glass slide, and the dye flows through the entire glass slide; the stepper motor of the drive unit drives the dyeing workbench to move to the third working position to complete the dyeing of dye B, that is, the dyeing process is ended; it returns to the second working position again, and the third peristaltic pump works to push clean water to spray out from the five-head dropper to flush out the excess dye remaining on the glass slide, thereby realizing the cleaning process.

[0022] Furthermore, the box is provided with an air drying unit and a touch display screen. The air drying unit includes two fans, which are placed above the slide rail. After the staining and washing steps are completed, the fans are started to dry the slide.

[0023] Furthermore, the box body is provided with ventilation holes to achieve air circulation in the box.

[0024] The working principle of the present invention is:

[0025] The staining workbench extends out of the through hole, that is, it is in the first working position. The user places the five slides to be processed on the slide in turn and waits for staining. Operate the touch display screen to start staining. The staining workbench moves along the slide rail toward the inside of the box, and at the same time, the staining workbench is lifted to a certain angle until it reaches the second working position. The first peristaltic pump pumps out the dye from the five-head dropper, so that the dye A soaks the glass slide, moves to the third working position, and stands for a period of time. After completing the staining of dye A, return to the second working position. The staining of dye B is completed by the same principle. The third peristaltic pump works to rinse the glass slide. The air drying unit starts to complete the drying of the glass slide, the staining workbench returns to the first working position, and the processed glass slide is taken out.

[0026] Compared with the prior art, the present invention has the following advantages:

[0027] The present invention has a small volume, uses less dye, and has controlled dyeing time, thereby improving dyeing efficiency and dyeing quality. The inclination and horizontal conversion of the dyeing workbench is realized by a limit guide rail. When tilted, the dye solution infiltrates the glass slide, and when horizontal, the dye is left to stand, thereby saving the amount of dye and controlling the dyeing time. Only one stepper motor is used to drive the device to work normally, thereby reducing the size of the device. In the process of endoscopic diagnosis and treatment, automatic glass slide dyeing is realized, thereby improving the dyeing efficiency and dyeing quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0029] Figure 2 This is a schematic diagram of the box cover structure of the present invention;

[0030] Figure 3 This is a schematic diagram of the structure of the dyeing component of the present invention;

[0031] Figure 4 This is a structural schematic diagram of the dyeing workbench of the present invention;

[0032] Figure 5 This is a schematic diagram of the structure of the connection unit of the present invention;

[0033] Figure 6 This is a schematic diagram of the automatic liquid supply unit of the present invention;

[0034] Figure 7 This is a schematic diagram of the first working position of the present invention;

[0035] Figure 8 This is a schematic diagram of the second working position of the present invention;

[0036] Fig. 9 This is a schematic diagram of the third working position of the present invention.

[0037] Figure numerals: 1-box, 11-through hole, 12-ventilation hole, 2-box cover, 3-dyeing assembly, 31-dyeing workbench, 311-fast dyeing table, 312-glass carrier, 313-spring sheet, 314-flow guide slope, 315-liquid outlet, 32-limiting unit, 321-slide plate, 322-limiting guide rail, 323-movable latch, 33-connecting unit, 331-rail hinge, 34-driving unit, 341-slider, 342-screw, 343-slide rail, 344-stepping motor, 4-automatic liquid supply unit, 41-dye bottle, 42-clean water tank, 43-peristaltic pump unit; 431-first peristaltic pump, 432-second peristaltic pump, 433-third peristaltic pump, 44-five-head dropper, 5-air drying unit, 6-touch display screen. DETAILED DESCRIPTION

[0038] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. Component models, material names, connection structures, control methods, algorithms and other features not clearly described in this technical solution are all considered to be common technical features disclosed in the prior art.

[0039] Example 1

[0040] This embodiment provides a slide staining device for rapid on-site evaluation, such as Figure 1 , 2 , 3, 4, 5, 6, comprising: a box body 1, a box body cover 2, and a dyeing component 3 and an automatic liquid supply unit 4 located inside the box body 1;

[0041] The dyeing assembly 3 includes a dyeing workbench 31, a limiting unit 32, a connecting unit 33 and a driving unit 34; the limiting unit 32 and the driving unit 34 are fixedly connected via the connecting unit 33, and the dyeing workbench 31 is fixedly connected to the limiting unit 32;

[0042] The automatic liquid supply unit 4 includes a dye bottle 41, a clean water tank 42, a peristaltic pump unit 43, and a five-head dropper 44. The peristaltic pump unit 43 includes multiple peristaltic pumps. Each dye bottle 41 is connected to a peristaltic pump, and the clean water tank 42 is connected to a peristaltic pump, and then merges into the five-head dropper 44. When replacing the dye or adding clean water, open the box cover 2, take out the dye bottle 41 and the clean water tank 42, and realize the addition and replacement of liquid.

[0043] like Figure 7 , 8 As shown in Figures 9 and 10, the limiting unit 32 includes a slide plate 321 and a limiting guide rail 322. The slide plate 321 is provided with a movable latch 323 to cooperate with the limiting guide rail 322. The limiting guide rail 322 has a first working position, a second working position and a third working position. A through hole 11 is provided on the front of the box body 1 for the dyeing workbench 31 to extend out. When the limiting guide rail 322 is located in the first working position, the dyeing workbench 31 extends out from the through hole 11, and the two fence hinges 331 are parallel to each other. When the limiting guide rail 322 is located in the second working position, the two fence hinges 331 are opened to a set angle, and the five-head dropper 44 is vertically aligned with one end of the dyeing workbench 31. When the limiting guide rail 322 is located in the third working position, the dyeing workbench 31 is located below the air-drying unit 5, and the two fence hinges 331 are parallel to each other.

[0044] In a specific embodiment, the connection unit 33 includes two fence hinges 331, the two fence hinges 331 are rotatably connected, and the fence hinges 331 are fixedly connected to the limiting unit 32 and the driving unit 34 respectively.

[0045] In a specific embodiment, the driving unit 34 includes: a slider 341, a lead screw 342, a slide rail 343 and a stepper motor 344. The slider 341 is arranged on the slide rail 343, and the slider 341 can slide along the slide rail 343. The stepper motor 344 drives the lead screw 342 to achieve linear movement; through the linear movement of the slider 341, the vertical lifting of the slide plate 321 is achieved, so as to achieve the purpose of increasing the angle between the two fence hinges 331.

[0046] In a specific embodiment, the staining workbench 31 includes a fast staining table 311 , a glass slide table 312 and two spring sheets 313 . The glass slide table 312 is provided with five glass slide slots and is fixed to the fast staining table 311 via two spring sheets 313 .

[0047] The limiting unit 32 is fixedly connected to the fast dyeing table 311 , so that when the driving unit 34 drives the dyeing table 31 to slide on the slide rail 343 , a certain angle can exist between the dyeing table 31 and the driving unit 34 .

[0048] In a specific embodiment, the rapid dyeing table 311 is provided with a liquid diversion slope 314, and liquid outlets 315 are provided on both sides of the rapid dyeing table 311. The diversion slope 314 and the liquid outlet 315 are used to concentrate and collect the outflowing waste liquid, which is convenient for subsequent cleaning and treatment.

[0049] In a specific embodiment, when the limiting guide rail 322 is in the first working position, the staining workbench 31 extends from the through hole 11, and the staining workbench 31 is parallel to the bottom surface of the box 1, and five slides to be processed are placed on the slide table 312 in sequence to wait for staining.

[0050] In a specific embodiment, the peristaltic pump unit 43 includes: a first peristaltic pump 431, a second peristaltic pump 432, and a third peristaltic pump 433;

[0051] When the limiting guide rail 322 is in the second working position, the five-head dropper 44 is vertically aligned with one end of the glass stage 312, and there is an angle between the dyeing workbench 31 and the bottom surface of the box 1, so that the liquid flows along the inclined surface;

[0052] The first peristaltic pump 431 works to push the dye solution A to the five-head dropper 44, and drops vertically onto the glass slide 312. The dyeing workbench 31 is an inclined plane in the second working position, and the dye solution flows through the entire glass slide due to gravity. The stepper motor 344 of the driving unit 34 then drives the dyeing workbench 31 to move to the third working position, and stands still for a period of time to complete the dyeing of the dye solution A. The horizontal third working position can save dye.

[0053] In a specific embodiment, after dyeing with dye A is completed, it returns to the second working position, and the second peristaltic pump 432 starts working to push dye B to the five-head dropper 44, and vertically drops to the glass stage 312, and the dye flows through the entire glass slide; the stepper motor 344 of the drive unit 34 drives the dyeing workbench 31 to move to the third working position to complete the dyeing of dye B, that is, the dyeing process is ended; it returns to the second working position again, and the third peristaltic pump 433 works to push clean water to spray out from the five-head dropper 44 to flush out the excess dye remaining on the glass slide, thereby completing the cleaning process.

[0054] In a specific embodiment, the box 1 is provided with an air drying unit 5 and a touch screen 6. The air drying unit 5 includes two fans, which are placed above the slide rail 343. After the staining and washing steps are completed, the fans are started to dry the slide. The touch screen 6 is arranged on the box cover 2, as shown in FIG. Figure 2 shown.

[0055] In a specific embodiment, the box body 1 is provided with ventilation holes 12 to achieve air circulation in the box.

[0056] The working principle of the present invention is:

[0057] The staining workbench 31 extends out from the through hole 11, that is, it is in the first working position. The user places the five slides to be processed on the slide table 312 in turn and waits for staining. Operate the touch display screen 66 to start staining. The staining workbench 31 moves along the slide rail 343 to the inside of the box 1, and at the same time, the staining workbench 31 is lifted by a certain angle until it reaches the second working position. The first peristaltic pump 431 pumps out the dye from the five-head dropper 44, so that the dye A soaks the glass slide, moves to the third working position, and stands for a period of time. After completing the staining of dye A, return to the second working position. The staining of dye B is completed by the same principle. The third peristaltic pump 433 works to rinse the glass slide. The air-drying unit 5 starts to complete the drying of the glass slide, the staining workbench 31 returns to the first working position, and the processed glass slide is taken out.

[0058] Components not described in detail in this embodiment are all existing components that can be purchased through public channels.

[0059] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A slide staining device for rapid on-site evaluation, characterized in that: include: A box body (1), a box body cover (2), and a dyeing component (3) and an automatic liquid supply unit (4) located inside the box body (1); The dyeing component (3) comprises a dyeing workbench (31), a limiting unit (32), a connecting unit (33) and a driving unit (34); the limiting unit (32) and the driving unit (34) are fixedly connected via the connecting unit (33), and the dyeing workbench (31) is fixedly connected to the limiting unit (32); The automatic liquid supply unit (4) comprises a dye bottle (41), a clean water tank (42), a peristaltic pump unit (43), and a five-head dropper (44); the peristaltic pump unit (43) comprises a plurality of peristaltic pumps, each dye bottle (41) is connected to a peristaltic pump, the clean water tank (42) is connected to a peristaltic pump, and then the liquids are fed into the five-head dropper (44); The limiting unit (32) comprises a slide plate (321) and a limiting guide rail (322); the slide plate (321) is provided with a movable latch (323) which cooperates with the limiting guide rail (322); the limiting guide rail (322) has a first working position, a second working position and a third working position: a through hole (11) for the dyeing workbench (31) to extend out is opened on the front of the box body (1); when the limiting guide rail (322) is located at the first working position, the dyeing workbench (31) extends out from the through hole (11); when the limiting guide rail (322) is located at the second working position, the five-head dropper (44) is vertically aligned with one end of the dyeing workbench (31); when the limiting guide rail (322) is located at the third working position, the dyeing workbench (31) is located below the air-drying unit (5).

2. A slide staining device for rapid on-site evaluation according to claim 1, characterized in that: The connection unit (33) comprises two guardrail hinges (331), the two guardrail hinges (331) are rotatably connected, and the guardrail hinges (331) are fixedly connected to the limiting unit (32) and the driving unit (34) respectively.

3. A slide staining device for rapid on-site evaluation according to claim 1, characterized in that: The driving unit (34) comprises: a slider (341), a lead screw (342), a slide rail (343) and a stepping motor (344); the slider (341) is arranged on the slide rail (343); the slider (341) can slide along the slide rail (343); the stepping motor (344) drives the lead screw (342) to achieve linear movement; and the slider (321) is lifted in the vertical direction through the linear movement of the slider (341).

4. The slide staining device for rapid on-site evaluation according to claim 1, characterized in that: The dyeing workbench (31) comprises a fast dyeing table (311), a glass slide table (312) and two spring sheets (313); the glass slide table (312) is provided with five glass slide slots and is fixed to the fast dyeing table (311) via the two spring sheets (313).

5. A slide staining device for rapid on-site evaluation according to claim 4, characterized in that: The rapid dyeing table (311) is provided with a liquid diversion slope (314), and liquid outlets (315) are provided on both sides of the rapid dyeing table (311). The outflowing waste liquid is concentrated and collected through the diversion slope (314) and the liquid outlet (315), which is convenient for subsequent cleaning and treatment.

6. The slide staining device for rapid on-site evaluation according to claim 4, characterized in that: When the limiting guide rail (322) is in the first working position, the dyeing workbench (31) extends out from the through hole (11), the dyeing workbench (31) is parallel to the bottom surface of the box body (1), and five slides to be processed are placed on the slide table (312) in sequence to wait for dyeing.

7. A slide staining device for rapid on-site evaluation according to claim 4, characterized in that: The peristaltic pump unit (43) comprises: a first peristaltic pump (431), a second peristaltic pump (432), and a third peristaltic pump (433); When the limiting guide rail (322) is in the second working position, the five-head dropper (44) is vertically aligned with one end of the glass carrier (312), and an angle exists between the dyeing workbench (31) and the bottom surface of the box (1), so that the liquid flows along the inclined surface; The first peristaltic pump (431) works to push the dye solution A to the five-head dropper (44), which vertically drops onto the glass slide (312). The dyeing workbench (31) is an inclined plane at the second working position, and the dye solution flows through the entire glass slide due to gravity. The stepper motor (344) of the drive unit (34) then drives the dyeing workbench (31) to move to the third working position, where it is left to stand for a period of time to complete the dyeing of the dye solution A. The horizontal third working position can save dye.

8. The slide staining device for rapid on-site evaluation according to claim 7, characterized in that: After dyeing with dye A is completed, it returns to the second working position, and the second peristaltic pump (432) starts to work, pushing dye B to the five-head dropper (44), and vertically drops onto the glass slide (312), and the dye flows through the entire glass slide; the stepper motor (344) of the drive unit (34) drives the dyeing workbench (31) to move to the third working position to complete the dyeing of dye B, that is, the dyeing process is completed; it returns to the second working position again, and the third peristaltic pump (433) starts to work, pushing clean water to spray out from the five-head dropper (44), flushing out the excess dye remaining on the glass slide, and completing the cleaning process.

9. The slide staining device for rapid on-site evaluation according to claim 1, characterized in that: The box body (1) is provided with an air drying unit (5) and a touch display screen (6).

10. The slide staining device for rapid on-site evaluation according to claim 1, characterized in that: The box body (1) is provided with ventilation holes (12) to enable the circulation of air in the box.

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