Essence for turning white hair into black hair and preparation method thereof

Abstract

The invention discloses essence for turning white hair into black hair and a preparation method of the essence. The raw materials such as the nasturtium officinale leaf / stem extract, the hydroxyferriheme, the polygonum multiflorum and the pseudo-ginseng are carefully selected, so that the hair follicle is activated, the vitality of melanocytes in the hair follicle is enhanced, and the melanogenesis is promoted. Tests of volunteers prove that along with the growth of new black hair and the shedding of aged white hair, the black hair is gradually increased and the white hair is gradually reduced when the essence for turning the white hair into the black hair is used for a long time, so that the sensory effect of turning the white hair into the black hair is realized.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, in particular to an essence liquid for converting white hair into black hair and a preparation method thereof. Background Art

[0002] Graying of hair is a complex process involving many factors. In most cases, it is a normal physiological phenomenon for hair to turn gray naturally with aging. However, factors such as the accelerated pace of life, increased work and mental stress, and lack of rest and sleep have caused an imbalance in the nutrition required for hair, resulting in an increasing number of young and middle-aged people with gray hair. Gray hair has become an important factor affecting people's image and mental and physical health, and has also become a key research object in the field of hair care.

[0003] Hair contains a large amount of melanin, which is synthesized by melanocytes in hair follicles. The melanin synthesis and metabolism process can be divided into the following stages: melanin synthesis in melanocytes; melanin particles are released from melanocytes by cell secretion at the dendrites of melanocytes and transferred to surrounding keratinocytes; melanin particles in keratinocytes melt. In the process of melanin particle generation, transfer and melting, any obstacle in any link can affect melanin metabolism, thus causing hair color changes.

[0004] Domestic and foreign research on gray hair mainly focuses on its etiology and pathogenesis. That is, most people believe that the reduction of tyrosinase activity is the main factor affecting the production and deposition of hair pigment. Reduced tyrosinase activity, reduced melanin synthesis, reduced pigmentation, and hair graying. At the same time, the interaction between melanin and keratinocytes is weakened, and the migration of melanocytes from the outer root sheath to the hair follicle papilla is reduced, which destroys the normal coloring function and can also cause hair graying. At the same time, the free radical theory is also the mechanism of hair graying, which is related to genetics, trace elements, vitamin abnormalities, etc.

[0005] The inventors of the present invention have discovered through research in the field of hair care that a combination of watercress leaf / stem extract, hematoxylin, Polygonum multiflorum, Panax notoginseng and other raw materials has a good effect on activating hair follicles, enhancing the activity of melanocytes in hair follicles and promoting melanin production. With the growth of new black hair and the shedding of old white hair, long-term use will gradually increase the number of black hair and reduce the number of white hair, thereby achieving the sensory effect of "white hair turning into black hair", thereby completing the present invention. Summary of the invention

[0006] The purpose of the present invention is to provide a white hair to black hair essence, which is compounded with watercress leaf / stem extract, hydroxyhemoglobin, Polygonum multiflorum, Panax notoginseng and other raw materials, and has good effects on activating hair follicles, enhancing the activity of melanocytes in hair follicles, and promoting melanin production. With the growth of new black hair and the shedding of aging white hair, long-term use will gradually increase the number of black hair and gradually reduce the number of white hair, thereby achieving the sensory effect of "white hair to black hair".

[0007] The specific technical solutions of the present invention are as follows:

[0008] A white hair to black hair essence contains the following components in percentage by mass: 1-10% of nasturtium officinale leaf / stem extract, 1-10% of hematoxylin, 1-10% of POLYGONUM MULTIFLORUM root extract, and 1-10% of PANAX NOTOGINSENG root extract.

[0009] In one embodiment, the white hair to black hair essence contains the following components in percentage by weight: 7% of NASTURTIUM OFFICINALE leaf / stem extract, 5% of hematoxylin, 3% of POLYGONUMMULTIFLORUM root extract, and 3% of PANAX NOTOGINSENG root extract.

[0010] Preferably, the white hair to black hair essence may also contain the following components in percentage by weight: 1-10% moisturizer and 0.1-5% fat excipient. The purpose of adding the moisturizer and fat excipient is to keep the moisture on the upper layer of the scalp for a long time, maintain and preserve the hydrolipid film on the surface of the scalp, and build a healthy environment for the scalp.

[0011] The moisturizing agent described in the present invention can be a polyol such as glycerol, butylene glycol, polyethylene glycol, propylene glycol, hexylene glycol, xylitol, sorbitol, etc., or it can be a pseudo-natural moisturizing factor such as amino acid, pyrrolidone carboxylic acid, sodium lactate, urea, ceramide, etc., or it can be a sugar such as methyl glucose polyether, chitin derivatives, trehalose, tremella polysaccharide, sodium hyaluronate, sugar isomers, etc., or it can be a multifunctional active ingredient such as panthenol and silk protein with moisturizing and nourishing effects.

[0012] The fat excipient of the present invention can be one or a mixture of silicone oil, vegetable oil, animal oil, synthetic oil.

[0013] Preferably, the white hair to black hair essence may also contain the following components in percentage by weight: 0.1-1% of a heat-sensing agent. The heat-sensing agent may produce a warm feeling, making people feel more comfortable. It may also stimulate blood vessel dilation, accelerate blood circulation, promote body metabolism, and facilitate the absorption of other active ingredients.

[0014] The heat-sensing agent of the present invention can be capsaicin, capsicum extract, vanillyl butyl ether, gingerol, ginger extract, etc.

[0015] Preferably, the white hair to black hair essence may also contain the following components in percentage by weight: 0.1-1% of anti-dandruff agent. The anti-dandruff agent can inhibit and kill fungi and bacteria, soften the stratum corneum, promote the exfoliation of the stratum corneum, etc., can remove dandruff, relieve scalp itching, improve scalp oil secretion, and promote healthy hair growth.

[0016] The anti-dandruff agent of the present invention can be an artificial synthetic / semi-synthetic raw material such as selenium disulfide, salicylic acid, pyrithione zinc, dipotassium glycyrrhizinate, climbazole, piroctone olamine salt, etc., or it can be a plant extract with anti-dandruff effect obtained by extraction, purification and compounding of tea tree, scutellaria baicalensis, coptis chinensis, cnidium monnieri, white moss peel, stemona radix, dandelion, agrimony and / or sophora flavescens.

[0017] Preferably, the white hair to black hair essence may also contain the following components in percentage by weight: 0.1-1% of an anti-allergic agent. The anti-allergic agent has the functions and effects of inhibiting the release of histamine, relieving itching, anti-inflammatory, and improving mucosal congestion, and can relieve scalp discomfort, eliminate inflammation, and promote healthy hair growth. The anti-allergic agent may be one or a mixture of bisabolol, purslane extract, chamomile extract, oat kernel extract, sodium guaiacyl sulfonate, etc.

[0018] The technical solution provided by the embodiment of the present invention has the following beneficial effects:

[0019] (1) The present invention follows the monarch-minister-assistant-assistant scheme, and selects watercress leaf / stem extract as the monarch drug, hematin and Polygonum multiflorum root extract as the ministerial drugs, and Panax notoginseng root extract as the assistant drug. Among them, watercress leaf / stem extract can activate hair follicles, improve gray hair, and promote hair growth; hematin can repair hair and promote the production of melanin; Polygonum multiflorum root extract can enhance the activity of melanocytes in hair follicles and promote the production of melanin in hair follicles; Panax notoginseng root extract can reduce inflammation, resist environmental stress, and protect hair follicle cells. The four active ingredients are used together, which has a good effect on activating hair follicles, enhancing the activity of melanocytes in hair follicles, and promoting melanin production.

[0020] (2) The embodiment of the white hair to black hair essence of the present invention also adds a moisturizer, a fat-forming agent, a thermal agent, an anti-dandruff agent and an anti-allergic agent, which can effectively maintain and preserve the hydrolipid film on the surface of the scalp, stimulate blood vessel dilation, promote the absorption of active ingredients, relieve scalp discomfort, improve scalp oil secretion, effectively build a healthy environment for the scalp, and promote healthy hair growth. Volunteer trials have proved that after long-term use of the white hair to black hair essence provided by the embodiment of the present invention, the volunteers' black hair gradually increased and the white hair gradually decreased, achieving the sensory effect of "white hair to black hair". BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for describing the embodiments or the prior art are briefly introduced below.

[0022] Figure 1 Shown are comparison photos of the volunteers in Experiment 3 before and after using the white hair to black hair essence. DETAILED DESCRIPTION

[0023] The following describes the embodiments of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0024] Before further describing the specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the examples of the present invention are for describing specific embodiments rather than for limiting the scope of protection of the present invention.

[0025] When the embodiment gives a numerical range, it should be understood that, unless otherwise specified in the present invention, the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those of ordinary skill in the art to which the present invention belongs.

[0026] It is worth noting that the raw materials used in the present invention are all common commercially available products, so their sources are not specifically limited.

[0027] The nasturtium officinale leaf / stem extract of the present invention is extracted from the above-ground part of the perennial aquatic herb nasturtium of the genus nasturtium of the cruciferous family. The nasturtium has a crisp and tender taste, is sweet and delicious, and can be cooked into various dishes. The whole plant can be used as medicine, and has the effects of clearing the lungs, cooling the blood, diuresis, and detoxification. Modern research has found that the nasturtium leaf / stem extract can activate hair follicles, improve gray hair, and promote hair growth. Suitable nasturtium officinale leaf / stem extracts can include BURGEON-UP (from ICHIMARU PHARCO Co., Ltd, Japan).

[0028] The hematin of the present invention is a raw material that can be used for Japanese quasi-drugs, has the function of promoting tyrosinase activity, and has an iron atom in the center of its porphyrin structure, which can combine with histidine and cysteine ​​in hair keratin, has strong adhesion to hair and exerts a repairing effect on hair. Suitable hematin can include GROWTHPHYLLIN P (PF) (from Ichimaru Co., Ltd., Japan).

[0029] The root extract of Polygonum multiflorum described in the present invention is extracted from the root of Polygonum multiflorum, a plant of the Polygonaceae family. Polygonum multiflorum has the effects of nourishing essence and blood (prepared); detoxifying, stopping malaria, and moistening the intestines and relieving constipation (raw use). It is mainly used to treat deficiency of essence and blood, dizziness, premature graying of hair, soreness of waist and knees, long-term malaria, carbuncle, scrofula, and constipation caused by dry intestines. The source of the root extract of Polygonum multiflorum can be a common commercial product. Suitable root extract of Polygonum multiflorum can include Polygonum multiflorum extract SHOU WU PE (from Guilin Rhine Biotechnology Co., Ltd.).

[0030] The Panax notoginseng root extract of the present invention is extracted from the root of Panax notoginseng, a perennial herb of the genus Panax of the Araliaceae family. Panax notoginseng is warm in nature, sweet and slightly bitter in taste. It enters the liver and stomach meridians. It has the effects of nourishing blood, resisting inflammation, promoting blood circulation and removing blood stasis, and can resist oxidation and anti-aging. The source of the Panax notoginseng root extract can be a common commercial product. Suitable Panax notoginseng root extracts can include Panax notoginseng extract (from Kunshan Jialong Biotechnology Co., Ltd.).

[0031] Experiment 1: Detection of intracellular melanin content and tyrosinase activity

[0032] This experiment is specially designed to verify the ability of the white hair to black hair essence provided by the present invention to enhance the activity of melanocytes in hair follicles and promote the production of melanin.

[0033] Mouse melanoma cell B16F10 is a commonly used melanin research model with low cost, strong repeatability and short experimental cycle. The experiment was carried out according to the method described in the paper "Huang Jie, et al. Synergistic whitening, antioxidant and anti-inflammatory activity of glabridin-resveratrol-polydatin composition [J]. Flavors and Fragrances Cosmetics, 2024, (03): 99-107+233." to determine the melanin content and tyrosinase activity.

[0034] Specific steps: B16F10 were inoculated in 6-well plates (1×10 5Each well was divided into two groups (cells / well), and different test substances were added on the next day. A negative control group was set up (PBS was used to replace the test substance). After 48 hours of culture, cells were collected for subsequent determination, and the final results were calibrated with cell counts. The concentration of the test substances was diluted to 0.1‰ with PBS. The inhibition rate was the ratio of the difference between the results of the control group and the test group to the result of the control group.

[0035] (1) Melanin content: Cell pellets were collected from each well and 0.4 mL of 1 mol / L NaOH solution (containing 10% DMSO by volume) was added, incubated at 80° C. for 30 min, and the absorbance was measured at 490 nm.

[0036] (2) Tyrosinase activity: Cell pellets were collected from each well and 0.5 mL of 0.5% sodium deoxycholate solution was added. The cells were lysed on ice for 15 min, then rewarmed at 37°C and 0.25 mL of 0.3% DOPA solution was added. The cells were reacted at 37°C for 30 min and the absorbance was measured at 475 nm.

[0037] Table 1 Effects of the test substances on melanin content and tyrosinase activity

[0038]

[0039] As can be seen from Table 1, the melanin inhibition rate and tyrosinase inhibition rate of each test group are negative, indicating that they all show the effect of activating tyrosinase and promoting melanin production. Among the five test groups, test group 1, which was added with NASTURTIUM OFFICINALE leaf / stem extract, hematoxylin, POLYGONUM MULTIFLORUM root extract, and PANAX NOTOGINSENG root extract, showed better effects.

[0040] Test 2 Chicken embryo chorioallantoic membrane test

[0041] The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. Since the surface of the chicken embryo chorioallantoic membrane is rich in blood vessels and can be regarded as a complete organism, this test uses the characteristics of the complete, clear and transparent chorioallantoic membrane system of the incubated chicken embryo in the middle stage. A certain amount of the test substance is directly in contact with the chicken embryo chorioallantoic membrane. After a specified time, the changes in the toxic effect indicators of the chorioallantoic membrane (such as bleeding, coagulation and vascular melting) are observed, and scores are given. The mathematical mean is calculated to evaluate the irritation of the test substance. The test was conducted with reference to the method described in the literature "Wang Xianhao, et al. Study on the soothing and repairing effects of a combination of two plant extracts on the skin [J]. Shandong Chemical Industry, 2024, 53(05): 43-45+50."

[0042] The test steps are as follows: SPF eggs are placed in a constant temperature incubator at 37.5±0.5℃ and relative humidity of 55-70% to hatch until 9 days old. The negative control is a 0.9% sodium chloride solution prepared with deionized water, which is used after sterilization. The positive control is a sodium dodecyl sulfate (SLS) solution, which is prepared with normal saline, and the final test concentration is 1% by mass. Take 0.3mL of normal saline and 1% SLS solution, respectively, and drop the test samples (NASTURTIUM OFFICINALE leaf / stem extract, hematoxylin, Polygonum multiflorum root extract, Panax notoginseng root extract in a ratio of 1:1:1:1) on the surface of CAM, observe the reaction of CAM, and record the time of vascular bleeding, coagulation, and vascular lysis within 300s, and calculate the irritation score (IS) and classification. There are 6 replicates in each treatment group.

[0043] IS < 1, non-irritating;

[0044] 1≤IS<5, mild irritation;

[0045] 5≤IS<9, moderate irritation;

[0046] IS ≥ 9, severe irritation.

[0047] IS = (301-bleeding time)*5 / 300+(301-bleeding thawing time)*5 / 300+(301-clotting time)*9 / 300.

[0048] The test results show that after the SLS solution was treated with CAM, obvious bleeding occurred within 300 seconds, and the IS value was 12.38, which was severely irritating; after the test sample was treated with CAM, no bleeding was observed within 300 seconds, and the IS value was 0, which showed no irritation. It can be seen that the active ingredients of the white hair to black hair essence of the present invention are mild and do not show irritation.

[0049] Example 1

[0050] A white hair to black hair essence contains the following components in percentage by mass: 1% of nasturtium officinale leaf / stem extract, 10% of hydroxyhemoglobin, 1% of POLYGONUM MULTIFLORUM root extract, 10% of PANAX NOTOGINSENG root extract, 1% of 1,3-propylene glycol, 5% of bis-aminopropyl polydimethylsiloxane, 0.1% of ginger extract, 1% of piroctone olamine salt, 0.1% of purslane extract, 1% of 1,2-pentanediol, 0.4% of p-hydroxyacetophenone, and the balance of water.

[0051] Example 2

[0052] A white hair to black hair essence contains the following components in percentage by mass: 10% of nasturtium officinale leaf / stem extract, 1% of hydroxyhemoglobin, 10% of POLYGONUM MULTIFLORUM root extract, 1% of PANAX NOTOGINSENG root extract, 10% of 1,3-propylene glycol, 0.1% of bis-aminopropyl polydimethylsiloxane, 1% of ginger extract, 0.1% of piroctone olamine salt, 1% of purslane extract, 0.5% of 1,2-pentanediol, 0.6% of p-hydroxyacetophenone, and the balance of water.

[0053] Example 3

[0054] A white hair to black hair essence contains the following components in percentage by mass: 7% of nasturtium officinale leaf / stem extract, 5% of hydroxyhemoglobin, 3% of POLYGONUM MULTIFLORUM root extract, 3% of PANAX NOTOGINSENG root extract, 5% of 1,3-propylene glycol, 2.5% of bis-aminopropyl polydimethylsiloxane, 0.5% of ginger extract, 0.5% of piroctone olamine salt, 0.5% of purslane extract, 0.8% of 1,2-pentanediol, 0.5% of p-hydroxyacetophenone, and the balance of water.

[0055] Trial 3 Volunteer Trial Test

[0056] 60 volunteers with gray hair problems, regardless of gender, aged 30-50 years old, were recruited and randomly divided into 2 groups, 30 in each group, the first group tested the gray hair to black hair essence prepared in Example 3, and the second group tested a commercially available brand of black hair essence.

[0057] Usage: Put the essence into a dropper bottle, wash and dry your hair, then use a dropper to drop 5-15 mL of the product on the gray hair, comb it evenly with a comb. Use once in the morning and once in the evening for 3 consecutive months. Conduct a follow-up visit in the first, second and third months, collect the self-evaluation results of the volunteers, and calculate the average score of each group.

[0058] The self-assessment criteria for volunteers’ improvement in gray hair: 0 points for those with no obvious effect in turning gray hair into black hair; 3 points for those with moderate improvement, who feel that they have less gray hair; 5 points for those with very good improvement, who feel that their gray hair has obviously decreased and their black hair has increased.

[0059] Table 2 Self-evaluation results of volunteers' improvement of gray hair

[0060] Month 1 Month 2 Month 3 Example 3 2.15±0.56 3.07±1.11 4.68±0.87 Commercially available products 1.02±0.12 1.87±1.02 3.12±1.23

[0061] As can be seen from Table 2, the "white hair to black hair" effect of the white hair to black hair essence prepared in the embodiment of the present invention is significantly better than that of commercially available products, and the effect becomes better and better with the extension of the use time, achieving excellent technical effects.

[0062] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Any simple modification or equivalent substitution made by any technician familiar with the technical field within the technical scope disclosed by the present invention does not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A white hair to black hair essence, characterized in that: The invention contains the following components in percentage by mass: 1-10% of NASTURTIUM OFFICINALE leaf / stem extract, 1-10% of hematin, 1-10% of POLYGONUMMULTIFLORUM root extract and 1-10% of PANAX NOTOGINSENG root extract.

2. The essence for turning white hair into black hair according to claim 1, characterized in that: The composition comprises the following components in percentage by weight: 7% of NASTURTIUM OFFICINALE leaf / stem extract, 5% of hematin, 3% of POLYGONUM MULTIFLORUM root extract, and 3% of PANAX NOTOGINSENG root extract.

3. The white hair to black hair essence according to claim 1 or 2, characterized in that: The invention also contains the following components in percentage by weight: 1-10% of a moisturizing agent and 0.1-5% of a fat excipient.

4. The essence for turning white hair into black hair according to claim 3, characterized in that: The moisturizing agent is one or a mixture of more than one of polyols, pseudo-natural moisturizing factors, sugars, and multifunctional active ingredients with moisturizing and nutritional properties.

5. The essence for turning white hair into black hair according to claim 3, characterized in that: The fat excipient is one or a mixture of more than one of silicone oil, vegetable oil, animal oil and synthetic oil.

6. The essence for turning white hair into black hair according to claim 1 or 2, characterized in that: The invention also contains the following components in percentage by weight: 0.1-1% of thermal sensitizer.

7. The essence for converting white hair to black hair according to claim 6, characterized in that: The heat-sensing agent is one or a mixture of more than one of capsaicin, capsicum extract, vanillyl butyl ether, gingerol and ginger extract.

8. The essence for turning white hair into black hair according to claim 1 or 2, characterized in that: The invention also contains the following components in percentage by weight: 0.1-1% of anti-dandruff agent.

9. The essence for converting white hair to black hair according to claim 8, characterized in that: The antidandruff agent is one or a mixture of more than one of selenium disulfide, salicylic acid, pyrithione zinc, dipotassium glycyrrhizinate, climbazole, piroctone ethanolamine salt, tea tree, scutellaria baicalensis, coptis chinensis, cnidium monnieri, white moss peel, stemona, dandelion, agrimony and sophora flavescens.

10. The essence for turning white hair into black hair according to claim 1 or 2, characterized in that: The invention also contains the following components in percentage by weight: 0.1-1% of anti-allergic agent; the anti-allergic agent is one or a mixture of bisabolol, purslane extract, chamomile extract, oat kernel extract and sodium guaiacyl sulfonate.

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