Anti-wrinkle composition containing recombinant collagen as well as preparation method and application of anti-wrinkle composition

Abstract

The invention relates to the field of cosmetics, and discloses an anti-wrinkle composition containing recombinant collagen as well as a preparation method and application of the anti-wrinkle composition. The anti-wrinkle composition is prepared by compounding I-type collagen peptide nano-micelles, recombinant humanized III-type collagen, recombinant humanized XVII-type collagen, glycosylglycerol and ceramide. The skin care composition has the advantages that multiple synergistic effects are achieved, collagen generation is promoted, the skin moisturizing capacity is enhanced, and the skin barrier is repaired, so that the elasticity, compactness and smoothness of the skin are remarkably improved. The product disclosed by the invention has the effects of skin barrier repair, anti-aging care, collagen protection, collagen generation promotion, water locking and moisturizing and the like, and is suitable for research, development and application of skin care products for resisting wrinkles, tightening and delaying senescence.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics and skin care products, and in particular to an anti-wrinkle composition containing recombinant collagen, and a preparation method and application thereof. Background Art

[0002] As we age, the amount of collagen in our skin decreases, causing it to lose elasticity, sag, and develop wrinkles. Collagen is a key protein that maintains skin firmness and elasticity, especially collagen types I, III, and XVII. Collagen type I is primarily responsible for maintaining the structural support of the skin, collagen type III helps improve skin elasticity, and collagen type XVII is responsible for strengthening the connection between the epidermis and dermis and improving skin firmness. However, with natural aging and the influence of the external environment, the skin's ability to produce these collagens decreases, collagen loss intensifies, and the skin becomes loose and wrinkles appear.

[0003] Currently, many skin care products on the market focus on supplementing collagen or simply moisturizing effects, but traditional collagen supplementation methods often have difficulty penetrating deep into the skin, and due to problems with its source and structure, it is easy to cause allergic reactions or cannot be well integrated with the body's own collagen. In addition, most of these products use animal-derived collagen, which has problems such as poor biocompatibility and low absorption efficiency, affecting the final anti-wrinkle effect.

[0004] Therefore, how to supplement and generate collagen through more effective and safer ways has become a key technical challenge in the field of skin care. Summary of the invention

[0005] The purpose of the present invention is to provide an anti-wrinkle composition containing recombinant collagen, and a preparation method and application thereof. Through the composite combination of type I collagen peptide nano-micelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glycerol glucoside and ceramide, multiple synergistic effects are achieved to promote collagen production, enhance the skin's moisturizing ability, and repair the skin barrier, thereby significantly improving the skin's elasticity, firmness and smoothness, effectively combating skin aging, and well overcoming the difficult problem of balancing skin transdermal properties, efficacy and safety of traditional anti-aging products, and fundamentally solving the problem of skin aging.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides an anti-wrinkle composition containing recombinant collagen, comprising the following components in mass percentage: 0.01% to 20% type I collagen peptide nano-micelles, 0.01% to 20% recombinant humanized type III collagen, 0.01% to 10% recombinant humanized type XVII collagen, 0.01% to 20% glyceryl glucoside and 0.01% to 5% ceramide.

[0008] Furthermore, the anti-wrinkle composition containing recombinant collagen comprises the following components in percentage by mass: 0.01% to 10% of type I collagen peptide nano-micelles, 0.01% to 8% of recombinant humanized type III collagen, 0.01% to 2% of recombinant humanized type XVII collagen, 0.01% to 10% of glyceryl glucoside and 0.01% to 2% of ceramide.

[0009] Furthermore, the anti-wrinkle composition containing recombinant collagen comprises the following components in mass percentage: 0.01% to 5% of type I collagen peptide nano-micelles, 0.01% to 3% of recombinant humanized type III collagen, 0.01% to 1% of recombinant humanized type XVII collagen, 0.01% to 5% of glyceryl glucoside and 0.01% to 0.5% of ceramide.

[0010] The anti-wrinkle composition containing recombinant collagen provided by the present invention is prepared by compounding type I collagen peptide nano-micelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glyceryl glucoside, and ceramide, and has the following effects:

[0011] 1. Multiple synergistic effects

[0012] (1) Supplement collagen and strengthen skin structure

[0013] Type I collagen peptide nano-micelles enhance skin support and slow down skin sagging; recombinant humanized type III collagen enhances skin elasticity, making the skin firmer and more elastic; recombinant humanized type XVII collagen strengthens the connection between the epidermis and dermis, enhancing the overall firmness of the skin.

[0014] (2) Improve moisturizing ability and lock collagen

[0015] Glyceryl glucoside can activate aquaporins (AQP) in the skin, promote the transport and retention of water, and enhance the hydration of the skin. By locking in moisture, it not only improves the smoothness of the skin, but also helps maintain the stability of collagen and prevents its loss.

[0016] (3) Repair skin barrier and protect collagen

[0017] Ceramide can repair the skin barrier, strengthen the skin's defense function, and prevent the external environment from eroding collagen. It repairs the damaged skin barrier, reduces water evaporation, maintains the activity of collagen in the skin, and makes the anti-wrinkle effect more lasting.

[0018] 2. Excellent biocompatibility

[0019] The amino acid sequence of recombinant humanized collagen is highly similar to human collagen, and almost completely matches the natural collagen structure of human skin, so it has extremely high biocompatibility. This means that it can be well absorbed by the skin, avoiding the immune response or allergy problems that may be caused by traditional animal-derived collagen. The present invention uses recombinant humanized collagen to significantly improve the integration efficiency of collagen in the skin, thereby achieving a more natural and long-lasting anti-wrinkle effect.

[0020] 3. Enhanced absorption and penetration capabilities

[0021] Traditional collagen has difficulty penetrating the surface of the skin due to its large molecular weight, which limits its effect in the deeper layers of the skin. However, recombinant humanized collagen has been optimized and has a better molecular structure and molecular weight design. It can more easily penetrate into the deeper layers of the skin and effectively play a role in the dermis, promoting the production and repair of collagen, and improving aging problems such as skin sagging and wrinkles.

[0022] Nano-micelle technology: Type I collagen peptide can greatly improve its transdermal permeability in the form of nano-micelle carriers. This is because the nano-micelles have a small particle size (usually between tens and hundreds of nanometers), which can break through the skin barrier and directly transport active ingredients to the deep layers of the skin, especially the dermis, thereby exerting stronger biological activity.

[0023] 4. Enhance skin moisturizing ability and repair skin barrier

[0024] Glyceryl Glucoside: As a natural moisturizing factor, Glyceryl Glucoside can enhance the hydration of the skin, improve the skin's moisturizing ability, help restore the skin's water-oil balance, and it can form a good barrier with the hydration in the skin to keep the skin soft and moisturized.

[0025] Ceramide: As an important skin barrier component, ceramide can repair damaged skin barriers, prevent moisture loss and resist external stimuli. Moreover, it is essential to strengthen the barrier function of the stratum corneum and can effectively prevent skin dryness, allergies and other problems.

[0026] The dual effects of glyceryl glucoside and ceramide can not only enhance skin hydration and reduce dryness problems common in the aging process, but also repair damaged skin barriers and improve the skin's ability to self-repair and resist external stimuli.

[0027] 5. Ensure safety and long-term effectiveness

[0028] Safety of nano-micelle system: The active ingredients are encapsulated in the nano-micelle system, avoiding adverse reactions (such as allergies, irritation, etc.) that may be caused by direct contact with the skin surface. Nano-micelles can form a protective film on the skin surface, allowing the active ingredients to be released slowly and improving their bioavailability in the skin.

[0029] At the same time, through the fine control of nano-micelle technology, the product can achieve long-lasting moisturizing and anti-aging effects, avoiding the problem of frequent application of traditional skin care products, thereby improving ease of use and durability of effects.

[0030] 6. Innovative anti-wrinkle composition to solve existing market pain points

[0031] Multi-dimensional treatment of skin aging: Most of the existing anti-aging products on the market focus on a single aspect (such as anti-oxidation, moisturizing, firming, etc.), while the present invention adopts a composite solution of multiple collagens, combined with glyceryl glucoside and ceramide, which can synergistically act at multiple levels and aspects to comprehensively improve the various manifestations of skin aging (such as sagging, dryness, fine lines, etc.).

[0032] Long-term effects and non-invasiveness: Compared with traditional invasive treatments (such as botulinum toxin injections, fillers, etc.), this composite product can be used externally to deeply repair the skin for a long time and continuously, avoiding the risks and side effects of traumatic treatments.

[0033] It can be seen that the present invention solves the challenges of traditional anti-aging products in terms of transdermal permeability, efficacy and safety by adopting a composite combination of multiple ingredients such as type I collagen peptide nano-micelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glycerol glucoside and ceramide, and provides a multi-synergistic solution that can effectively promote the production of collagen, enhance the skin's moisturizing ability, repair the skin barrier, and improve signs of aging. This innovative technology not only has a significant anti-aging effect, but also ensures the safety of use and the durability of the effect, filling the technical gap in the market for anti-aging products.

[0034] In some embodiments, the anti-wrinkle composition containing recombinant collagen further comprises one or a combination of two or more of an emulsifier, an antioxidant, an emollient, a thickener, a moisturizer, a chelating agent, a skin conditioner, a flavoring agent, a preservative, and a pH adjuster, and the balance is water. In addition, other functional ingredients may also be included as needed.

[0035] Specifically, based on the total mass of the anti-wrinkle composition containing recombinant collagen as 100%, the content of the emulsifier is 0-20%, the content of the antioxidant is 0-10%, the content of the emollient is 0-50%, the content of the thickener is 0.01%-8%, the content of the moisturizer is 0.01%-40%, the content of the chelating agent is 0.01%-1%, the content of the skin conditioner is 0-50%, the content of the flavoring agent is 0-1%, the content of the preservative is 0.01-3%, and the content of the pH regulator is 0-3%.

[0036] More specifically, based on the total mass of the anti-wrinkle composition containing recombinant collagen as 100%, the content of the emulsifier is preferably 0-10%, the content of the antioxidant is preferably 0-5%, the content of the emollient is preferably 0-20%, the content of the thickener is preferably 0.01%-1%, the content of the moisturizer is preferably 0.01%-20%, the content of the chelating agent is preferably 0.01%-0.08%, the content of the skin conditioner is preferably 0-20%, the content of the flavoring agent is preferably 0-0.2%, the content of the preservative is preferably 0.01-2%, and the content of the pH regulator is preferably 0-2%.

[0037] Furthermore, the emulsifier is selected from one or more of PEG-100 stearate, polyglyceryl-3 methylglucose distearate, potassium cetyl phosphate, cetearyl glucoside, cetearyl olivetate, sodium methyl stearoyl taurate, sodium methyl stearoyl taurate, hydrogenated lecithin, sorbitan olivetate and polyglyceryl-3 distearate. It should be noted that the emulsifier may be any emulsifier that can form a water-in-oil (W / O) type or an oil-in-water (O / W) type emulsified anti-wrinkle cosmetic composition without any particular limitation.

[0038] In some embodiments, the emulsifier is selected from one or more of cetearyl glucoside, potassium cetyl phosphate, and hydrogenated lecithin.

[0039] Furthermore, the antioxidant is selected from one or more of vitamin E and its derivatives, vitamin C and its derivatives, ubiquinone and fullerene.

[0040] In some embodiments, the antioxidant is selected from vitamin E and its derivatives.

[0041] Further, the emollient is selected from one or more of tri(ethylhexanoic acid)glyceryl, caprylic / capric triglyceride, pentaerythritol tetra(ethylhexanoic acid) ester, butyloctanol salicylate, diethoxyethyl succinate, isododecane, rice germ oil, hydrogenated palm kernel oil, cocoa seed butter cetyl ethylhexanoate, isopropyl myristate, isopropyl palmitate, diisopropyl sebacate, polydimethylsiloxane, cetearyl alcohol, dibutyl adipate, C12-15 alcohol benzoate, dicaprylyl carbonate, coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, octyl polymethylsiloxane, cyclopentasiloxane and phenyl trimethyl polysiloxane. It should be noted that as the emollient, other emollients commonly used in the cosmetics field can be used without particular limitation.

[0042] In some embodiments, the emollient is selected from one or more of C12-15 alcohol benzoate, dimethicone, and rice germ oil.

[0043] Furthermore, as the aqueous phase component, the thickener is selected from one or more of xanthan gum, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, carbomer, ammonium acryloyldimethyltaurate / VP copolymer, sodium acrylate / sodium acryloyldimethyltaurate copolymer.

[0044] In some embodiments, the thickener is selected from one or more of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, ammonium acryloyldimethyl taurate / VP copolymer, sodium acrylate / sodium acryloyldimethyl taurate copolymer.

[0045] Furthermore, as the aqueous phase component, any moisturizer commonly used in the cosmetics field can be used without particular limitation, and the moisturizer is selected from one or more of glycerin, propylene glycol, seaweed extract, sodium hyaluronate, aloe extract, lubricant oil, polyethylene glycol / polypropylene glycol-14 / 7 dimethyl ether, xylitol, and sorbitol.

[0046] In some embodiments, the humectant is selected from glycerin.

[0047] Furthermore, as the aqueous phase component, the chelating agent is one or a combination of two or more of disodium EDTA, tetrasodium EDTA, and sodium phytate.

[0048] Furthermore, the skin conditioning agent is selected from one or more of vitamin E, scutellaria baicalensis extract, bisabolol, scutellaria baicalensis root extract, prinus utilis extract, glucan, and vitamin B5, which have the function of repairing damaged skin.

[0049] Furthermore, the flavoring agent includes one or both of essence and essential oil.

[0050] Furthermore, as a water phase component, the preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, hexylene glycol, and pentylene glycol.

[0051] Furthermore, as the aqueous phase component, the pH adjuster is selected from one or more of arginine, sodium hydroxide, potassium hydroxide, triethanolamine, and aminomethyl propanol.

[0052] In a second aspect, the present invention provides a method for preparing the above-mentioned anti-wrinkle composition containing recombinant collagen, comprising the following steps:

[0053] S1. Weigh each component according to the formula;

[0054] S2. Dissolve the aqueous phase components at 80°C to 90°C and stir until there are no particles;

[0055] S3. After cooling to below 45° C., add the remaining components and stir evenly to prepare an anti-wrinkle composition containing recombinant collagen.

[0056] In a third aspect, the present invention also provides the use of the above-mentioned anti-wrinkle composition containing recombinant collagen in the preparation of cosmetics.

[0057] In some embodiments, the cosmetic is any one of skin care lotion, essence, lotion, face cream, face mask, sunscreen, BB cream, and isolation cream.

[0058] Compared with the prior art, the present invention provides an anti-wrinkle composition containing recombinant collagen and a preparation method and application thereof, which have the following beneficial effects:

[0059] The present invention achieves multiple synergistic effects through the composite combination of type I collagen peptide nano-micelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glycerol glucoside and ceramide, promotes collagen production, enhances the skin's moisturizing ability, and repairs the skin barrier, thereby significantly improving the skin's elasticity, firmness and smoothness. The product of the present invention has the effects of skin barrier repair, anti-aging care, collagen protection, collagen production promotion, water locking and moisturizing, and is suitable for the research and development and application of anti-wrinkle, firming and anti-aging skin care products. DETAILED DESCRIPTION

[0060] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0061] In the embodiments and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used can be purchased from conventional raw material manufacturers unless otherwise specified.

[0062] Type I collagen peptide nano-micelles are currently available and can be purchased from Shaanxi Future Peptide Biotechnology Co., Ltd. Collagen peptides.

[0063] The present invention is further described in detail below through detailed embodiments, comparative examples and experimental tests.

[0064] 1. Application Examples

[0065] Example 1

[0066] This embodiment provides an anti-wrinkle composition containing recombinant collagen, which includes the following components by mass percentage:

[0067] Phase B: Type I collagen peptide nanomicelles 0.05%, recombinant humanized type III collagen 0.05%, recombinant humanized type XVII collagen 0.05%, glyceryl glucoside 0.05%, preservative 0.5%;

[0068] Phase A: 0.02% ceramide, 0.05% chelating agent, 0.5% thickener, 5% humectant and the balance deionized water.

[0069] Among them, the chelating agent is EDTA-2Na, the thickener is sodium acrylate / sodium acryloyldimethyl taurate copolymer, the moisturizer is glycerin, and the preservative is phenoxyethanol.

[0070] The specific preparation method is as follows:

[0071] S1. Weigh each component according to the formula;

[0072] S2. Dissolve the components of phase A at 85°C and stir until there are no particles;

[0073] S3. After cooling to below 45° C., add the phase B component and stir evenly to prepare an anti-wrinkle composition.

[0074] Example 2

[0075] This embodiment provides an anti-wrinkle composition containing recombinant collagen, which is different from Example 1 in that the specific dosage of the components is different, and the other components remain unchanged. For specific changes in the dosage of each component, please refer to Table 1 below.

[0076] Example 3

[0077] This embodiment provides an anti-wrinkle composition containing recombinant collagen, which is different from Example 1 in that the specific dosage of the components is different, and the other components remain unchanged. For specific changes in the dosage of each component, please refer to Table 1 below.

[0078] Example 4

[0079] This embodiment provides an anti-wrinkle composition containing recombinant collagen, which is different from Example 1 in that the specific dosage of the components is different, and the other components remain unchanged. For specific changes in the dosage of each component, please refer to Table 1 below.

[0080] Example 5

[0081] This embodiment provides an anti-wrinkle composition containing recombinant collagen, which is different from Example 1 in that the specific dosage of the components is different, and the other components remain unchanged. For specific changes in the dosage of each component, please refer to Table 1 below.

[0082] Example 6

[0083] This embodiment provides an anti-wrinkle composition containing recombinant collagen, which is different from Example 1 in that the specific dosage of the components is different, and the other components remain unchanged. For specific changes in the dosage of each component, please refer to Table 1 below.

[0084] Table 1 Formulation of Examples 1 to 6

[0085]

[0086] 2. Application comparison

[0087] Based on Example 3, multiple comparative examples, namely, Comparative Examples 1 to 34, are set up around the five important components, namely, type I collagen peptide nanomicelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glyceryl glucoside, and ceramide, by eliminating one or more components or replacing a certain component.

[0088] Among them, in Comparative Examples 1 to 5, any four of the five components, namely, type I collagen peptide nano-micelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glyceryl glucoside, and ceramide, are selected for setting.

[0089] Comparative Examples 6 to 15 are prepared by selecting any three of the five components: type I collagen peptide nanomicelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glyceryl glucoside, and ceramide.

[0090] Comparative Examples 16 to 24 are prepared by selecting any two of the five components: type I collagen peptide nanomicelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glyceryl glucoside, and ceramide.

[0091] Comparative Examples 25 to 29 are prepared by selecting one of the five components: type I collagen peptide nanomicelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glyceryl glucoside, and ceramide.

[0092] Comparative Examples 30 to 34 respectively replace the five components of type I collagen peptide nanomicelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glyceryl glucoside, and ceramide with conventional raw materials, that is, Comparative Example 30 uses conventional animal-derived type I collagen to replace type I collagen peptide nanomicelles, Comparative Example 31 uses conventional animal-derived type III collagen to replace recombinant humanized type III collagen, Comparative Example 32 uses conventional animal-derived type XVII collagen to replace recombinant humanized type XVII collagen, Comparative Example 33 uses conventional glycerol to replace glyceryl glucoside, and Comparative Example 34 uses conventional panthenol to replace ceramide.

[0093] 3. Experimental Test

[0094] Experiment 1: Elastase activity inhibition test

[0095] Elastase activity inhibition test is of great significance in anti-aging research because it directly evaluates the ability of skin care products to inhibit the degradation of elastin. Elastin is a key component in the skin that maintains elasticity and firmness. Although it accounts for a small proportion in the dermis, it plays an important role in maintaining skin elasticity and preventing wrinkles and sagging. However, with age, elastin is gradually broken down by elastase, causing the skin to lose elasticity and develop aging phenomena such as wrinkles and sagging. Through the elastase activity inhibition test, researchers can evaluate the inhibitory effect of active ingredients in skin care products on elastase activity. This test can reveal whether the skin care composition effectively prevents or slows the degradation of elastin, thereby reflecting the product's potential to maintain skin elasticity and delay aging. Inhibiting elastase activity is an important mechanism for delaying wrinkle formation and maintaining skin firmness, so it has key evaluation value in anti-aging research.

[0096] Therefore, the elastase activity inhibition test was used, and the specific test process was as follows:

[0097] Take the compositions prepared in Examples 1 to 6 and Comparative Examples 1 to 34, dilute them with deionized water to a sample solution with a concentration of 5%, and keep them for later use. Add 1 mL of the sample solution and 1 mL of the elastase solution to a 5 ml centrifuge tube, mix thoroughly, and react at 37 ° C for 5 min, then add 1 mL of the substrate solution, and continue to react at 37 ° C for 30 min; after incubation, centrifuge at 1000 rpm for 5 min, draw 200 uL of the supernatant into a 96-well plate, and detect the absorbance at a wavelength of 405 nm with an enzyme reader. At the same time, set up a blank substrate group (replace the substrate with an equal volume of 10% DMSO), a 100% enzyme activity control group, and a blank control group. Three parallel experiments were performed in each group, with sivelestat sodium as a positive control. The calculation formula for the elastase inhibition rate is as follows:

[0098] Elastase inhibition rate = [1-(Y1-Y2) / (Y3-Y4)] x 100%

[0099] Wherein: Y1 is the absorbance value of the test sample + elastase solution + substrate solution, Y2 is the absorbance value of the test sample + elastase solution + 10% DMSO, Y3 is the absorbance value of the PBS buffer solution + elastase solution + substrate solution, and Y4 is the absorbance value of the PBS buffer solution + elastase solution + 10% DMSO. The results of the elastase activity inhibition test are shown in Table 2 below.

[0100] Table 2 Elastase activity inhibition test results

[0101]

[0102]

[0103] As can be seen from Table 2, the anti-wrinkle compositions obtained in Examples 1 to 6 all have good elastase activity inhibition ability, and the highest clearance rate reaches 89.67%. Among them, the anti-wrinkle composition obtained in Examples 1 to 6 has a much stronger ability to inhibit elastase activity than the effect of using a single component alone in Comparative Examples 25 to 29, and is also much stronger than the effect of using any four components in Comparative Examples 1 to 5, any three components in Comparative Examples 6 to 15, and any two components in Comparative Examples 16 to 24. The composition obtained in Comparative Examples 30 to 34, due to the use of components with similar efficacy to replace a component in the composition of the present invention, its elastase activity inhibition ability is also much lower than the anti-wrinkle composition obtained in Examples 1 to 6 of the present invention. Therefore, it is further explained that the components in the anti-wrinkle composition provided by the present invention have a synergistic effect, and the five components of type I collagen peptide nano-micelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glyceryl glucoside, and ceramide are indispensable, and the composition of the present invention has a strong ability to inhibit elastase activity.

[0104] Experiment 2: Analysis of Transepidermal Water Loss Rate

[0105] Trans-epidermal water loss (TEWL) is measured by Tewameter, TM300, and is an important parameter that reflects skin water loss and barrier function. The lower the measured value, the better the skin barrier function. The detection method for trans-epidermal water loss analysis is as follows:

[0106] Subject screening criteria: healthy female subjects aged between 20 and 50 years old; and the subjects' skin tested positive after a 10% lactic acid sting test (determined to be sensitive skin); and there was slight redness on the face; 85 subjects, the compositions obtained from Examples 1 to 6 and Comparative Examples 1 to 10 were taken as samples for use evaluation, and 5 subjects were randomly selected for each sample.

[0107] Application area: face

[0108] Frequency of use: Use once in the morning and once in the evening for 28 consecutive days, 1.2g each time.

[0109] Test environment conditions: temperature 21±1℃, relative humidity 50±5%.

[0110] Experimental design: Single-blind, randomized, self-controlled before and after, face (the side opposite to the area where the lactic acid solution was applied was selected according to the random table).

[0111] Evaluation method details: Skin transepidermal water loss rate analysis, the results are shown in Table 3 below.

[0112] Table 3 Facial skin transepidermal water loss change rate data

[0113]

[0114]

[0115] From the results in Table 3, it can be seen that in Examples 1 to 6, as the mass percentage of type I collagen peptide nano-micelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glycerol glucoside and ceramide in the formula gradually increases, the transepidermal water loss of facial skin is improved. From Example 3 and Comparative Examples 1 to 10, it can be seen that the combination of type I collagen peptide nano-micelles, recombinant humanized type III collagen, recombinant humanized type XVII collagen, glycerol glucoside and ceramide can achieve better effects than a single or simple combination, and achieves the effects of moisturizing and barrier repair.

[0116] The above embodiments are merely illustrative of the concept and technical solution of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or change the above embodiments without violating the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by a person with ordinary knowledge in the technical field without departing from the spirit and technical ideas disclosed by the present invention should still be covered by the claims of the present invention.

[0117] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each implementation mode may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. An anti-wrinkle composition containing recombinant collagen, characterized in that: The invention comprises the following components in percentage by mass: 0.01% to 20% of type I collagen peptide nano micelles, 0.01% to 20% of recombinant humanized type III collagen, 0.01% to 10% of recombinant humanized type XVII collagen, 0.01% to 20% of glyceryl glucoside and 0.01% to 5% of ceramide.

2. The anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that: The invention comprises the following components in percentage by mass: 0.01% to 10% of type I collagen peptide nano micelles, 0.01% to 8% of recombinant humanized type III collagen, 0.01% to 2% of recombinant humanized type XVII collagen, 0.01% to 10% of glyceryl glucoside and 0.01% to 2% of ceramide.

3. The anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that: The invention comprises the following components in percentage by weight: 0.01% to 5% of type I collagen peptide nano-micelles, 0.01% to 3% of recombinant humanized type III collagen, 0.01% to 1% of recombinant humanized type XVII collagen, 0.01% to 5% of glyceryl glucoside and 0.01% to 0.5% of ceramide.

4. The anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that: It also includes one or a combination of two or more of an emulsifier, an antioxidant, a moisturizer, a thickener, a humectant, a chelating agent, a skin conditioner, a flavoring agent, a preservative, and a pH adjuster, and the balance is water.

5. The anti-wrinkle composition containing recombinant collagen according to claim 4, characterized in that: Based on the total mass of the anti-wrinkle composition as 100%, the content of the emulsifier is 0-20%, the content of the antioxidant is 0-10%, the content of the emollient is 0-50%, the content of the thickener is 0.01%-8%, the content of the humectant is 0.01%-40%, the content of the chelating agent is 0.01%-1%, the content of the skin conditioner is 0-50%, the content of the flavoring agent is 0-1%, the content of the preservative is 0.01-3%, and the content of the pH regulator is 0-3%.

6. The anti-wrinkle composition containing recombinant collagen according to claim 5, characterized in that: Based on the total mass of the anti-wrinkle composition as 100%, the content of the emulsifier is 0-10%, the content of the antioxidant is 0-5%, the content of the emollient is 0-20%, the content of the thickener is 0.01%-1%, the content of the humectant is 0.01%-20%, the content of the chelating agent is 0.01%-0.08%, the content of the skin conditioner is 0-20%, the content of the flavoring agent is 0-0.2%, the content of the preservative is 0.01-2%, and the content of the pH regulator is 0-2%.

7. The anti-wrinkle composition containing recombinant collagen according to claim 4, characterized in that: The emulsifier is selected from one or more of PEG-100 stearate, polyglyceryl-3 methyl glucose distearate, potassium cetyl phosphate, cetearyl glucoside, cetearyl olivetate, sodium methyl stearoyl taurate, sodium methyl stearoyl taurate, hydrogenated lecithin, sorbitan olivetate and polyglyceryl-3 distearate; The antioxidant is selected from one or more of vitamin E and its derivatives, vitamin C and its derivatives, ubiquinone and fullerene; The emollient is selected from one or more of tri(ethylhexanoin)glyceryl, caprylic / capric triglyceride, pentaerythritol tetra(ethylhexanoin), butyloctanol salicylate, diethoxyethyl succinate, isododecane, rice germ oil, hydrogenated palm kernel oil, cocoa seed butter cetyl ethylhexanoate, isopropyl myristate, isopropyl palmitate, diisopropyl sebacate, polydimethylsiloxane, cetearyl alcohol, dibutyl adipate, C12-15 alcohol benzoate, dicaprylyl carbonate, coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, octyl polymethylsiloxane, cyclopentasiloxane and phenyl trimethicone; The thickener is selected from one or more of xanthan gum, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, carbomer, ammonium acryloyldimethyl taurate / VP copolymer, sodium acrylate / sodium acryloyldimethyl taurate copolymer; The moisturizer is selected from one or more of glycerin, propylene glycol, seaweed extract, sodium hyaluronate, aloe extract, lubricant oil, polyethylene glycol / polypropylene glycol-14 / 7 dimethyl ether, xylitol, and sorbitol; The chelating agent is one or a combination of two or more of disodium EDTA, tetrasodium EDTA and sodium phytate; The skin conditioning agent is selected from one or more of vitamin E, scutellaria baicalensis extract, bisabolol, scutellaria baicalensis root extract, prickle fruit extract, glucan, and vitamin B5; The fragrance agent includes one or both of essence and essential oil; The preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, hexylene glycol, and pentylene glycol; The pH regulator is selected from one or more of arginine, sodium hydroxide, potassium hydroxide, triethanolamine, and aminomethyl propanol.

8. The method for preparing an anti-wrinkle composition containing recombinant collagen according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1. Weigh each component according to the formula; S2. Dissolve the aqueous phase components at 80°C to 90°C and stir until there are no particles; S3. After cooling to below 45° C., add the remaining components and stir evenly to prepare an anti-wrinkle composition containing recombinant collagen.

9. Use of the anti-wrinkle composition containing recombinant collagen according to any one of claims 1 to 7 in the preparation of cosmetics.

10. The use according to claim 9, characterized in that: The cosmetic is any one of skin care lotion, essence, emulsion, facial cream, facial mask, sunscreen, BB cream, and isolation cream.

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