Method for culturing dry moss by using brine

Abstract

The invention relates to a method for culturing dry moss by using brine, and belongs to the technical field of artificial culture of marine green algae, and the method comprises the following steps: preparing a brine solution; the method comprises the following steps: selecting dry moss frond in a good growth state, cleaning, cutting the cleaned dry moss frond into algae sections, and uniformly putting the algae sections into a container filled with a brine solution and a substratum; stimulating the dry moss frond in the container to release spores / gametes by increasing illumination or increasing temperature; after the spores / gametes are attached to the substratum, the substratum is placed in aquaculture water for aquaculture culture; harvesting the algae when the algae cultured on the substratum reach a proper growth amount. The method is easy to operate, the yield and quality of the dried moss are improved by manually controlling the breeding environment, meanwhile, brine resources and the salt pan mud flat area are effectively utilized, and good ecological benefits and economic benefits are achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of artificial cultivation of marine green algae, and in particular relates to a method for cultivating dry moss by utilizing brine. Background Art

[0002] Dry moss, as a general name for large green algae such as Enteromorpha sphaerocephala, Enteromorpha sphaerocephala, Enteromorpha sphaerocephala, Enteromorpha sphaerocephala, Enteromorpha sphaerocephala, etc. of the Ulvaceae family, is widely distributed in rocky marshes or mudflats in the middle and low tide areas along the coast of my country. Dry moss algae are rich in protein, essential amino acids, flavor amino acids, unsaturated fatty acids, minerals and vitamins, and are a type of economic seaweed with high nutritional value. In addition, it is also a material of medicinal and edible origin. The Tang Dynasty's "Sea Medicine Materia Medica", the Ming Dynasty's "Compendium of Materia Medica", and the Qing Dynasty's "Sui Xi Ju Diet" all have records about the medicinal value of dry moss, such as expectoration, detoxification and swelling.

[0003] At present, food and health products processed from dried moss have been widely favored by the market. However, the biomass of dried moss mostly depends on the natural growth environment. The supply of wild algae is difficult to meet the demand every year, and the quality is also greatly affected by environmental factors. Therefore, artificial breeding is needed, but due to the complex offshore breeding environment and many interference factors, artificial breeding is difficult.

[0004] my country is rich in brine resources, especially the underground brine in coastal areas, which provides unique resource conditions for Laizhou Bay, Bohai Bay, Liaodong Bay coast, coastal plain area and Yellow River Delta plain area in northern coastal areas of my country. These areas rely on the geographical advantages of being close to the sea, vast tidal flat resources and rich brine resources to form many large salt fields, providing good conditions for the production of salt industry and related industrial products.

[0005] Therefore, it is particularly necessary to develop artificial cultivation of dry moss using salt pan brine. Summary of the invention

[0006] The technical problem to be solved by the present invention is to provide a method for cultivating dry moss by utilizing brine.

[0007] In order to solve the above technical problems, the technical solution adopted by the present invention is: a method for cultivating dry moss using brine, comprising the following steps: S1. Prepare brine solution with a concentration of 20‰~40‰ for standby use; S2, select dry moss algae in good growth state for cleaning, cut the cleaned dry moss algae into algae segments, and evenly place them in a container filled with 20‰~40‰ brine solution and attachment base; S3, stimulate the dry moss algae in the container to release spores / gametes by increasing light or raising temperature; S4, after the spores / gametes are attached to the attachment base, the attachment base is placed in aquaculture water for aquaculture and culture; S5. Harvest when the algae cultured on the attachment substrate reaches the set growth amount.

[0008] Furthermore, in step S1, the brine solution is prepared by preparing brine from a salt lake, salt pond or salt pan.

[0009] Further, in step S2, the dry moss algae body is Enteromorpha, Enteromorpha flat, Enteromorpha curvilineara, Enteromorpha marginale, Enteromorpha stripes or green algae.

[0010] Furthermore, in step S2, the cleaned dry moss algae bodies are cut into algae segments of 0.01 cm to 1 cm.

[0011] Furthermore, in step S2, the attachment base is a nylon rope, a mesh curtain, a gauze, a gauze or a filter cotton.

[0012] Further, in step S3, the dried moss algae are placed in a strong light to induce spore / gamete release, and the strong light is 100-200 μmol photons m -2 s -1 ; Or increase the culture temperature by 2℃~5℃ to induce spore / gamete release.

[0013] Furthermore, in step S4, the aquaculture water body is a natural or artificial salt lake, salt pond or salt pan.

[0014] Furthermore, in step S4, during the culture and cultivation process, water quality indicators are regularly monitored and nutrients are supplemented.

[0015] Furthermore, in step S4, during the culture and cultivation process, water quality indicators are monitored once a week, and the water quality indicators include salinity, pH value, and nutrient salt content; the supplemented nutrients include carbon, nitrogen, phosphorus, and iron.

[0016] The beneficial effects of the present invention are: The invention has the advantages of simple operation and extensive cultivation, and the yield and quality of dry moss are improved by artificially selecting dry moss varieties and controlling the breeding environment, thereby realizing the comprehensive utilization of brine resources.

[0017] The present invention utilizes brine as a breeding water source, which not only saves seawater resources and offshore breeding area, but also fully utilizes brine resources and tidal flat area.

[0018] The present invention ensures the healthy growth of dry moss and improves the safety of the product through measures such as water quality control and light management.

[0019] It can be seen that the cultivation method of the present invention can not only meet the market demand for dry moss, but also realize the effective utilization of brine resources and salt pan tidal flat area. At the same time, during the cultivation process, dry moss will secrete sugars, proteins, amino acids, flavor nucleotides and other substances into the environment, which can enrich the nutritional components of brine water bodies such as salt pans, add unique flavor and nutritional value to salt products, form new products with market competitiveness, and have good ecological and economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention will be described in detail below with reference to the accompanying drawings and in combination with examples, and the advantages and implementation methods of the present invention will become more obvious. The contents shown in the accompanying drawings are only used to explain the present invention and do not constitute any limitation to the present invention in any sense. In the accompanying drawings: Figure 1 This is a growth photo of the dry moss algae body according to Example 1 of the present invention. DETAILED DESCRIPTION

[0021] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and the best embodiments.

[0022] Site selection: Choose a coastal or inland salt pan or an artificial pond with similar conditions to ensure clean water and sufficient sunlight.

[0023] A method for cultivating dry moss using brine comprises the following steps: S1. Prepare brine solution of appropriate concentration using brine from natural or artificial salt lakes, salt ponds or salt pans, and prepare the brine solution with a concentration of 20‰~40‰ for standby use; Specifically, the concentration of the brine solution is adjusted by adding fresh water, and the preferred concentration is 25‰~35‰; the concentration of the brine solution is measured by a salinometer or a hydrometer.

[0024] S2. Select dry mosses (Enteromorpha serrata, Enteromorpha serrata, Enteromorpha flexuosa, Enteromorpha fringeata, Enteromorpha stripes, green algae, etc.) in good growth state for cleaning, cut the cleaned dry mosses into 0.01 cm to 1 cm segments, and evenly place them in a container containing brine solution and attachment base of appropriate concentration; Specifically, when the temperature of the brine solution is 25°C ~ 35°C, the selectable types of dry moss include Enteromorpha quli, Enteromorpha, green algae, etc.; when the temperature of the brine solution is 10°C ~ 24°C, the selectable types of dry moss include Enteromorpha serrata, Enteromorpha flat, Enteromorpha stripes, Enteromorpha fringetuberis, Enteromorpha quli, green algae, etc.; the attachment base is nylon rope, mesh curtain, gauze, gauze or filter cotton.

[0025] The dry moss algae are cut into 0.01cm~1cm algae segments with scissors, which is conducive to the formation of sporangia / gametangia and the release of spores / gametes.

[0026] S3, stimulate the release of spores / gametes from dry moss algae by increasing light or raising temperature; Specifically, the dried moss was placed in a strong light environment to induce spore / gamete release. The strong light was 100-200 μmolphotons m -2 s -1 ; Or increase the regular culture temperature by 2℃~5℃.

[0027] Under the same conditions, sporophytes are more likely to release spores than gametes.

[0028] S4. After the spores / gametes to be released attach to the attachment base, the attachment base is placed in aquaculture water (natural or artificial salt lake, salt pond or salt pan) for aquaculture and cultivation. The water quality indicators are regularly monitored and adjusted, and nutrients are supplemented in a timely manner; Among them, for large-area aquaculture water bodies, it is more suitable to use net curtains as the attachment base of dry moss and algae; for small-area aquaculture water bodies or aquaculture water bodies with special purposes, it is more suitable to use nylon ropes, gauze, gauze or filter cotton as the attachment base of dry moss and algae.

[0029] Specifically, water quality indicators such as salinity, pH value, nutrient content, etc. are monitored once a week, and nutrients such as carbon, nitrogen, phosphorus, iron and other biogenic elements are added in a timely manner.

[0030] S5. The algae cultured on the attachment substrate are harvested when they reach an appropriate growth volume. The algae basal cells remaining on the attachment substrate can continue to grow and form new biomass.

[0031] Embodiment 1: The Enteromorpha samples were collected from the river channel of the sea cucumber breeding area in Rizhao, Shandong Province, the Enteromorpha samples came from laboratory culture, and the green algae (Ulva simplex) samples were collected from the waterway of Tianjin Changlu Hangu Salt Field Co., Ltd.

[0032] A method for cultivating dry moss using brine comprises the following steps: S1. Obtain brine from the salt fields of Tianjin Changlu Hangu Salt Field Co., Ltd. The salinity is measured to be 50‰. Add 3 volumes of brine to 2 volumes of fresh water to prepare a 30‰ brine solution for use.

[0033] S2. Select dark green and good-condition Enteromorpha algae bodies, wash them to remove the miscellaneous algae and other attachments, and place them in natural seawater for cultivation for later use. When using, cut the cleaned dry algae bodies into 0.01cm~1cm algae segments and evenly place them in a beaker containing 30‰ brine solution and nylon rope.

[0034] S3, placed at 80~120 μmol photons m -2 s-1 Treat under strong light for 2h~5h to stimulate the algae to release spores / gametes.

[0035] S4. After the spores / gametes to be released are attached to the nylon rope, the nylon rope with the spores / gametes attached is transferred to new brine for cultivation, and then transferred to natural light for cultivation (0d).

[0036] S5, such as Figure 1 As shown in the figure, after one week (7 days), a large number of seedlings were visible on the nylon rope, with a length of about 1 cm to 3 cm. After another two weeks (21 days), the algae grew rapidly, with a length of about 5 cm to 15 cm.

[0037] Embodiment 2: The Enteromorpha samples were collected from the river channel of the sea cucumber breeding area in Rizhao, Shandong Province, the Enteromorpha samples came from laboratory culture, and the green algae (Ulva simplex) samples were collected from the waterway of Tianjin Changlu Hangu Salt Field Co., Ltd.

[0038] A method for cultivating dry moss using brine comprises the following steps: S1. Obtain brine from the salt fields of Tianjin Changlu Hangu Salt Field Co., Ltd. The salinity is measured to be 50‰. Add 3 volumes of brine to 2 volumes of fresh water to prepare a 30‰ brine solution for use.

[0039] S2. Select Ulva simplex algae with dark green color and good condition, clean them to remove the miscellaneous algae and other attachments, and place them in natural seawater for cultivation. When using, cut the cleaned dry moss algae into 0.01cm~1cm algae segments and evenly place them in a beaker containing 30‰ brine solution and gauze.

[0040] S3, placed at 80~120 μmol photons m -2 s -1 Treat under strong light for 2h~5h to stimulate the algae to release spores / gametes.

[0041] S4. After the spores / gametes to be released are attached to the gauze, the gauze with the spores / gametes is transferred to new brine for cultivation, and then transferred to natural light for cultivation.

[0042] S5. After one week, a large number of seedlings can be seen on the gauze with the naked eye, with a length of about 1cm~3cm. After another two weeks, the algae grow rapidly, with a length of about 5cm~15cm.

[0043] The embodiments of the present invention are described in detail above, but the contents are only preferred embodiments of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the present invention.

Claims

1. A method for cultivating dry moss using brine, characterized in that: The steps include: S1. Prepare brine solution with a concentration of 20‰~40‰; S2, the dried moss algae are cleaned, and the cleaned dried moss algae are cut into algae segments, and evenly placed in a container filled with 20‰~40‰ brine solution and attachment base; S3, stimulate the dry moss algae in the container to release spores / gametes by increasing light or raising temperature; S4, after the spores / gametes are attached to the attachment base, the attachment base is placed in aquaculture water for aquaculture and culture; S5. Harvest when the algae cultured on the attachment substrate reaches the set growth amount.

2. The method for cultivating dry moss using brine according to claim 1, characterized in that: In step S1, the brine solution is prepared by preparing brine from a salt lake, salt pond or salt pan.

3. The method for cultivating dry moss using brine according to claim 1, characterized in that: In step S2, the dry moss algae body is Enteromorpha, Enteromorpha flat, Enteromorpha curvilineara, Enteromorpha marginale, Enteromorpha stripes or green algae.

4. The method for cultivating dry moss using brine according to claim 1, characterized in that: In step S2, the cleaned dry moss algae are cut into algae segments of 0.01 cm to 1 cm.

5. The method for cultivating dry moss using brine according to claim 1, characterized in that: In step S2, the attachment base is a nylon rope, a mesh curtain, a gauze, a gauze or a filter cotton.

6. The method for cultivating dry moss using brine according to claim 1, characterized in that: In step S3, the dried moss algae are placed in a strong light environment to induce spore / gamete release. The strong light intensity is 100-200 μmol photons m -2 s -1 ; Or increase the culture temperature by 2℃~5℃ to induce spore / gamete release.

7. The method for cultivating dry moss using brine according to claim 1, characterized in that: In step S4, the aquaculture water body is a natural or artificial salt lake, salt pond or salt pan.

8. The method for cultivating dry moss using brine according to claim 1, characterized in that: In step S4, during the culture and cultivation process, water quality indicators are regularly monitored and nutrients are supplemented.

9. The method for cultivating dry moss using brine according to claim 8, characterized in that: In step S4, during the culture and cultivation process, water quality indicators are monitored once a week, and the water quality indicators include salinity, pH value, and nutrient salt content; the supplemented nutrients include carbon, nitrogen, phosphorus, and iron.

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