Application of composition of herba houttuyniae and traditional Chinese medicine in preparation of food, medicine or health care product with anti-oxidation effect

Abstract

The invention relates to an application of a composition of herba houttuyniae and traditional Chinese medicines in preparation of food, medicines or health care products with an anti-oxidation effect. The composition comprises the following raw materials: houttuynia cordata, paniculate swallowwort root, dandelion, platycodon grandiflorum, American ginseng, bamboo fungus, exocarpium citri grandis, honeysuckle, momordica grosvenori, lily, red dates, lophatherum gracile, flos farfarae, scaphium scaphigerum, liquorice, plantain herb, medlar and mint. The composition has high in-vitro antioxidant activity, the highest scavenging rate of the composition to DPPH free radicals with the concentration of 50 mg / L can reach 89.04%, the highest scavenging rate to hydroxyl free radicals (OH.) is 30.65%, and the highest iron oxidation reduction capability (FRAP) is 0.93, so that the composition has the effects of retarding oxidative stress, resisting aging and preventing and treating chronic diseases, and can be used for preparing antioxidant drugs, health care products or food.

Description

Technical Field

[0001] The invention relates to a new application of a composition of Houttuynia cordata and traditional Chinese medicine, and in particular to an application of the composition of Houttuynia cordata and traditional Chinese medicine in preparing food, medicine or health food with antioxidant effect. Background Art

[0003] A series of diseases caused by oxidative stress and oxidative inflammation have become a major hidden danger threatening human health. The so-called oxidative stress refers to the imbalance between the production and elimination of reactive oxygen species (ROS) in the body. Long-term oxidative stress can lead to oxidative inflammation, which is closely related to a variety of human diseases, especially non-communicable chronic diseases such as cardiovascular and cerebrovascular diseases, cancer, chronic respiratory diseases, diabetes, etc. The appropriate intake of antioxidants can help the body get rid of oxidative stress, reduce oxidative damage, and prevent the occurrence of oxidative inflammation, which is of great significance for the treatment and prevention of human diseases.

[0004] There are many antioxidant products on the market. Among the commercially available products, vitamin C is currently recognized as one of the antioxidants with the best antioxidant activity and has been widely used in production and life. However, as a synthetic antioxidant, it has obvious side effects and has the potential risk of causing blood clots, stones and tumors in the human body. Therefore, it is very important to conduct in-depth research and development of natural, safe and effective antioxidant foods, health products and medicines through multiple channels. After thousands of years of practical testing by the Chinese nation, plants with medicinal and edible properties are known for their effectiveness, traceability and safety, and are ideal sources of antioxidants.

[0005] Houttuynia cordata Thunb, also known as Houttuynia cordata Thunb, is the rhizome of Houttuynia cordata of the Saururaceae family, and is certified as a rare variety of "drug and food". Houttuynia cordata is widely distributed in southwest my country, central China, Tibet, Shaanxi and other places, and likes to grow in moist streams, ridges and forests. It is not only commonly used to make cold dishes, but also in traditional Chinese medicine treatment, the dried Houttuynia cordata is boiled in water or brewed into tea, which has multiple effects such as clearing away heat and detoxification, expectorant and cough, analgesic and hemostatic. Studies have shown that Houttuynia cordata extract has significant therapeutic effects on diseases such as cancer, diabetes, obesity and gastric ulcers, and its water extract also shows strong anti-lipid peroxidation activity, comparable to vitamin C. However, at present, Houttuynia cordata, as a traditional Chinese medicinal material, has relevant research reports on its antioxidant activity, but there is no public report on its compatibility with the Chinese medicine composition in the present invention for antioxidant efficacy. Summary of the invention

[0006] In view of the limitations of current technologies, the present invention is committed to providing an innovative composition of Houttuynia cordata and traditional Chinese medicine and its preparation process. The composition not only combines the natural active ingredients of Houttuynia cordata, but also combines the unique efficacy of traditional Chinese medicine, has excellent antioxidant properties, and significantly improves its health care and therapeutic effects.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] The invention provides a composition of Houttuynia cordata and traditional Chinese medicine. The composition of Houttuynia cordata and traditional Chinese medicine is prepared from the following raw material components in parts by weight: 500-6000 parts of houttuynia cordata, 150-600 parts of Cynanchum chinense, 100-1500 parts of dandelions, 50-1500 parts of platycodon, 50-800 parts of American ginseng, 50-500 parts of bamboo fungus, 100-800 parts of tangerine peel, 100-2000 parts of honeysuckle, 50-500 parts of momordica grosvenori, 100-2500 parts of lily, 100-600 parts of red dates, 50-800 parts of loquat leaves, 50-1000 parts of coltsfoot flowers, 50-750 parts of Sterculia lychnophora, 100-600 parts of liquorice, 50-600 parts of plantain, 50-1000 parts of wolfberry and 50-1500 parts of mint.

[0009] Preferably, the composition of Houttuynia cordata and traditional Chinese medicine comprises the following raw materials: houttuynia, Cynanchum indica, dandelion, platycodon, American ginseng, bamboo mushroom, tangerine peel, honeysuckle, momordica grosvenori, lily, red dates, light bamboo leaves, coltsfoot flower, Sterculia lychnophora, licorice, plantain, wolfberry;

[0010] or houttuynia, dandelion, honeysuckle, mint, American ginseng, lily, bamboo leaf, coltsfoot, plantain;

[0011] Or Houttuynia cordata, Cynanchum chinense, honeysuckle, Platycodon grandiflorum, Plantain, American ginseng, wolfberry, dandelion, bamboo fungus.

[0012] The raw materials of the present invention respectively have the following effects and functions: a. Houttuynia cordata provides heat-clearing and detoxifying, antiviral and major antioxidant functions; b. Cynanchum cyrtonema has the effects of dispelling wind, removing dampness, relieving pain and relieving itching; c. Dandelion has the effects of clearing away heat and detoxifying, protecting the stomach and aiding digestion; d. Platycodon grandiflorum has the functions of clearing the lungs, relieving sore throat, removing phlegm and draining pus; e. American ginseng has the effects of replenishing qi and yin, clearing away heat and promoting body fluid; f. Bamboo mushrooms have the effects of clearing away heat and detoxifying, reducing swelling and relieving pain, and moistening the intestines and promoting bowel movements; g. Huajuhong has the effects of regulating qi, relieving fullness, drying dampness and resolving phlegm, and promoting digestion; h. Honeysuckle has the effects of clearing away heat, detoxifying, dispersing wind and dissipating heat; i. Monk fruit has the effects of clearing heat and moistening the lungs, relieving sore throat and opening voice, and moistening the intestines and relieving constipation; j. Lily has the effects of calming the mind, beautifying the skin, moisturizing and relieving cough; k. Red dates have the effect of replenishing qi, nourishing blood and calming the mind; l. Bamboo leaves have the effects of clearing away heat and purging fire, relieving restlessness and quenching thirst, and promoting diuresis and relieving stranguria; m. Coltsfoot has the effect of moistening the lungs, relieving qi, relieving cough and reducing phlegm; n. Sterculia lychnophora has the effects of clearing heat and moistening the lungs, relieving sore throat and opening the voice, and moistening the intestines and relieving constipation; o. Licorice has the functions of replenishing qi, tonifying the middle, removing phlegm and relieving cough, detoxifying, relieving urgency and relieving pain; p. Plantain has the effects of promoting diuresis, relieving stranguria, dispelling dampness and stopping diarrhea, improving eyesight, clearing the lungs and resolving phlegm; q. Wolfberry nourishes the liver and kidneys, improves intelligence and improves vision; r. Peppermint has the effects of dispersing wind-heat, clearing the head and eyes, relieving sore throat, clearing rashes, and soothing the liver and promoting qi.

[0013] Preferably, the composition of Houttuynia cordata and traditional Chinese medicine comprises the following raw materials in parts by weight: 500-6000 parts of Houttuynia cordata, 150-550 parts of Cynanchum wilfordii, 100-600 parts of Taraxacum mongolicum, 50-500 parts of Platycodon grandiflorum, 50-600 parts of American ginseng, 50-300 parts of Bambusa longituba, 100-800 parts of Citrus aurantium, 100-1000 parts of Honeysuckle, 50-500 parts of Momordica grosvenori, 100-800 parts of Lilium, 100-500 parts of Red Dates, 50-800 parts of Lophatherum gracile, 50-500 parts of Coltsfoot Flos, 50-500 parts of Sterculia lychnophora, 50-600 parts of Licorice, 50-500 parts of Plantain, and 50-500 parts of Lycium barbarum.

[0014] Or 500-6000 parts of houttuynia, 100-1250 parts of dandelions, 150-1800 parts of honeysuckle, 50-1500 parts of mint, 25-800 parts of American ginseng, 100-2000 parts of lilies, 50-1500 parts of lophatherum gracile, 50-1000 parts of coltsfoot flowers, and 50-500 parts of plantain.

[0015] Or 500-6000 parts of houttuynia, 25-800 parts of cynanchum chinense, 100-1000 parts of honeysuckle, 100-1250 parts of platycodon, 100-800 parts of plantain, 50-500 parts of American ginseng, 100-1000 parts of wolfberry, 100-800 parts of dandelion, and 50-500 parts of bamboo fungus.

[0016] Preferably, the auxiliary materials also include: white sugar and / or honey, and the white sugar and honey are used to adjust the taste of the refreshing drink.

[0017] The invention provides a preparation method of the above-mentioned Houttuynia cordata and traditional Chinese medicine composition, comprising the following steps: soaking Houttuynia cordata and its auxiliary materials in clear water respectively to obtain plant essence extract, mixing and boiling the extracts to obtain the Houttuynia cordata and traditional Chinese medicine composition.

[0018] The composition of Houttuynia cordata and traditional Chinese medicine of the present invention also includes auxiliary materials or auxiliary ingredients acceptable in food, medicine and health food.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The invention provides a combination of Houttuynia cordata and traditional Chinese medicine. The Houttuynia cordata is used as a main material and other auxiliary materials are combined. The Houttuynia cordata and traditional Chinese medicine combination has a scavenging ability of up to 89.04% for 50 mg / L DPPH free radicals, an in vitro scavenging ability of up to 30.65% for hydroxyl free radicals (·OH), a maximum ferric redox potential (FRAP) reduction ability of 0.93, and high antioxidant capacity. Antioxidant Houttuynia cordata and traditional Chinese medicine combinations with multiple flavors and functions are prepared according to the auxiliary materials, thereby enriching the types of antioxidant products and providing a new idea for preventing and eliminating oxidative stress diseases, namely, the integration of traditional medicinal materials and the diversification of therapeutic effects.

[0021] The invention provides a method for preparing the composition of the Houttuynia cordata and traditional Chinese medicine. The preparation is simple and convenient, the prepared product has good taste and has broad market prospects. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 Example 1 and Comparative Example 1, Example 2 and Comparative Example 2, Example 3 and Comparative Example 3 The results of the scavenging ability test on 50 mg / mL DPPH free radicals. Figure 2 Example 1 and Comparative Example 1, Example 2 and Comparative Example 2, Example 3 and Comparative Example 3 The results of the scavenging ability test for hydroxyl radicals (·OH) are shown in the figure. Figure 3 Example 1 and Comparative Example 1, Example 2 and Comparative Example 2, Example 3 and Comparative Example 3 Results of the ferrous redox ability (FRAP) assay. DETAILED DESCRIPTION

[0022] The invention provides a composition of Houttuynia cordata and traditional Chinese medicine. The composition of Houttuynia cordata and traditional Chinese medicine comprises the following raw materials: houttuynia cordata, Cynanchum chinense, dandelion, platycodon, American ginseng, bamboo mushroom, tangerine peel, honeysuckle, momordica grosvenori, lily, red dates, loquat leaf, coltsfoot flower, Sterculia lychnophora, liquorice, plantain, wolfberry and mint.

[0023] Unless otherwise specified, the present invention has no special requirements for the preparation raw materials, and commercially available products known to those skilled in the art can be used.

[0024] The Houttuynia cordata of the present invention can be collected in a well-known distribution area (widely distributed in southwest my country, central China, Tibet, Shaanxi, etc.) or purchased in a local market. In an embodiment of the present invention, the Houttuynia cordata was purchased from the Gucheng Market in Dali Town, Dali Bai Autonomous Prefecture, Yunnan Province from February to April 2024.

[0025] To further illustrate the present invention, the application of the composition of Houttuynia cordata and Chinese medicine provided by the present invention and the preparation method thereof in food, medicine or health care products is further described in detail below in conjunction with examples.

[0026] Example 1

[0027] (1) Raw material processing:

[0028] Select fresh Houttuynia cordata, wash it and drain the water; cut the Houttuynia cordata into small pieces of 1-5cm and set aside.

[0029] (2) Making Houttuynia cordata juice:

[0030] Put the reserved Houttuynia cordata in (1) into a pestle and pound it to extract juice; put the pounded Houttuynia cordata into a volumetric container, brew it with hot water for 30 minutes, then filter the Houttuynia cordata and keep the liquid for later use.

[0031] (3) Prepare auxiliary juice:

[0032] Mix Cynanchum chinense, dandelion, platycodon, American ginseng, bamboo mushroom, tangerine peel, honeysuckle, momordica grosvenori, lily, red dates, loquat leaves, coltsfoot, Sterculia lychnophora, licorice, plantain and wolfberry in a mass ratio of 1:1:1:1:0.5:1.5:1.5:1.5:1.5:1.5:1:1:1:1:1; add 20 times boiled mineral water to 105 g of the above mixed raw materials, soak for 30 min, and after solid-liquid separation, one of the preferred auxiliary materials can be obtained.

[0033] (4) Preparation:

[0034] The reserved houttuynia cordata juice in (2), the auxiliary materials prepared in (3) and honey are mixed in a ratio of 20:5:1, and 3 g of honey is added for seasoning to obtain the Houttuynia cordata and traditional Chinese medicine composition.

[0035] (5) Detection of the antioxidant capacity of the composition:

[0036] The combination of Houttuynia cordata and traditional Chinese medicine in Example 1 was poured into 50 mL centrifuge tubes respectively, and centrifuged at 5000 rpm / min for 15 min. Then 20 mL of sample was taken for dilution. After dilution into 6 concentration gradients, the following tests were performed.

[0037] DPPH scavenging ability in vitro:

[0038] Take 800 μL of sample supernatant with different concentration gradients and mix with 50 mg / L DPPH working solution in equal volume, react in a 37°C water bath in the dark for 30 minutes, and measure the absorbance at 517 nm. At the same time, take 800 μL of sample supernatant with different concentration gradients and mix with an equal volume of anhydrous ethanol as a control tube, and take 800 μL of anhydrous ethanol and mix with an equal volume of DPPH working solution as a blank tube. Determine its DPPH free radical scavenging ability according to the above sample method. Repeat the experiment 3 times.

[0039] DPPH free radical scavenging rate = {1-[(A1-A2) / A0]}×100%

[0040] Where A1 is the absorbance value of the sample tube; A2 is the absorbance value of the control tube; A0 is the absorbance value of the blank tube.

[0041] Hydroxyl radical (OH·) scavenging ability:

[0042] First, add 400μL of 3mmol / L FeSO4, 3mmol / L H2O2 solution and samples of different concentration gradients to the centrifuge tube, mix well, react in the dark for 10 minutes, add 400μL of 3mmol / L salicylic acid, mix well, react in the dark for 30 minutes, and measure the absorbance at 510nm. At the same time, take 400μL of anhydrous ethanol to replace salicylic acid as a control tube. Replace the samples of different concentration gradients with anhydrous ethanol, add 400μL of 3mmol / L FeSO4, 3mmol / L H2O2 solution and anhydrous ethanol respectively, mix well and react in the dark for 10 minutes, then add 400μL of 3mmol / L salicylic acid, mix well, react in the dark for 30 minutes as a blank tube, and repeat the experiment 3 times.

[0043] Hydroxyl radical (OH·) scavenging rate = {1-[(A1-A2) / A0]}×100%

[0044] Where A1 is the absorbance value of the sample tube; A2 is the absorbance value of the control tube; A0 is the absorbance value of the blank tube.

[0045] Ferric redox potential (FRAP) reduction capacity determination:

[0046] Take 50 μL of sample supernatant with different concentration gradients and mix with 1.2 mL of FRAP working solution, react in a 37°C water bath in the dark for 10 minutes, and measure the absorbance at 593 nm. At the same time, take 50 μL of sample supernatant with different concentration gradients and mix with 1.2 mL of deionized water as a control tube. Calculate the blank group according to the standard curve. Drawing of the standard curve: Use 0.1-1.6 mmol / L FeSO4 standard solution instead of the sample to draw the standard curve. Determine its FRAP reducing ability according to the above sample method. Repeat the experiment 3 times.

[0047] Results: The composition of Houttuynia cordata and Chinese medicine prepared in Example 1 has good antioxidant activity. Its scavenging ability for 50 mg / L DPPH free radicals can reach up to 57.14%, its scavenging ability for hydroxyl free radicals (OH·) can reach up to 20.26%, and its maximum reducing ability for iron redox potential (FRAP) is 0.92.

[0048] Example 2

[0049] (1) Raw material processing: same as in Example 1.

[0050] (2) Preparation of Houttuynia Cordata Juice: Same as Example 1.

[0051] (3) Prepare auxiliary juice:

[0052] Evenly mix dandelion, honeysuckle, mint, American ginseng, lily, bamboo leaves, coltsfoot and plantain in a mass ratio of 5:7:6:3:8:6:4:2; take 82g of the mixed raw materials, add 15 times of boiled mineral water, soak for 30 minutes, and after solid-liquid separation, one of the preferred auxiliary materials can be obtained.

[0053] (4) Preparation:

[0054] The reserved houttuynia cordata juice in (2), the auxiliary materials prepared in (3) and honey are mixed in a ratio of 50:20:1, and 1.5 g of honey is added for seasoning to obtain the Houttuynia cordata and traditional Chinese medicine composition.

[0055] (5) Detection of the antioxidant capacity of the composition: Same as Example 1.

[0056] Results: The composition of Houttuynia cordata and traditional Chinese medicine prepared in Example 2 has good antioxidant activity. Its scavenging ability for 50 mg / L DPPH free radicals can reach up to 49.72%, its scavenging ability for hydroxyl free radicals (OH·) can reach up to 30.65%, and its maximum reducing ability for iron redox potential (FRAP) is 0.45.

[0057] Example 3

[0058] (1) Raw material processing: same as in Example 1.

[0059] (2) Preparation of Houttuynia Cordata Juice: Same as Example 1.

[0060] (3) Prepare auxiliary juice:

[0061] Mix Cynanchum chinense, honeysuckle, platycodon, plantain, American ginseng, wolfberry, dandelion and bamboo mushroom in a mass ratio of 3:4:5:3:2:4:3:2; take 52g of the above mixed raw materials, add 10 times boiled mineral water, soak for 30 minutes, and after solid-liquid separation, you can get one of the preferred auxiliary materials.

[0062] (4) Preparation:

[0063] The reserved houttuynia cordata juice in (2) and the prepared auxiliary materials in (3) are mixed in a ratio of 5:2 to obtain the Houttuynia cordata and traditional Chinese medicine composition.

[0064] (5) Detection of the antioxidant capacity of the composition: Same as Example 1.

[0065] Results: The composition of Houttuynia cordata and Chinese medicine prepared in Example 3 has good antioxidant activity. Its scavenging ability for 50 mg / L DPPH free radicals can reach up to 89.04%, its scavenging ability for hydroxyl free radicals (OH·) can reach up to 22.20%, and its maximum reducing ability for iron redox potential (FRAP) is 0.93.

[0066] Comparative Example 1

[0067] The method of Example 1 is followed, except that Houttuynia cordata is omitted from the raw materials.

[0068] Comparative Example 2

[0069] The method of Example 2 is followed, except that Houttuynia cordata is omitted from the raw materials.

[0070] Comparative Example 3

[0071] The method of Example 3 was followed, except that Houttuynia cordata was omitted from the raw materials.

[0072] It is worth noting that the above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the invention patent. It should be pointed out that for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An application of a composition of Houttuynia cordata and Chinese medicine in the preparation of food, medicine or health care product with antioxidant effect, wherein the Houttuynia cordata is Houttuynia cordata, and the composition of Houttuynia cordata and Chinese medicine comprises the following raw materials in parts by weight: 500-6000 parts of Houttuynia cordata, 150-600 parts of Cynanchum indica, 100-1500 parts of Taraxacum mongolicum, 50-1500 parts of Platycodon grandiflorum, 50-800 parts of American ginseng, 50-500 parts of Bamboo mushroom 100-800 parts of Huajuhong, 100-2000 parts of honeysuckle, 50-500 parts of momordica, 100-2500 parts of lilies, 100-600 parts of red dates, 50-800 parts of light bamboo leaves, 50-1000 parts of coltsfoot flowers, 50-750 parts of sterculia lychnophora, 100-600 parts of liquorice, 50-600 parts of plantain, 50-1000 parts of wolfberry, and 50-1500 parts of mint.

2. The composition of Houttuynia cordata and traditional Chinese medicine according to claim 1, characterized in that: The composition of Houttuynia cordata and traditional Chinese medicine is prepared from the following raw material components in parts by weight: 500-6000 parts of houttuynia cordata, 150-550 parts of cynanchum chinense, 100-600 parts of dandelions, 50-500 parts of platycodon, 50-600 parts of American ginseng, 50-300 parts of bamboo fungus, 100-800 parts of tangerine peel, 100-1000 parts of honeysuckle, 50-500 parts of momordica grosvenori, 100-800 parts of lily, 100-500 parts of red dates, 50-800 parts of bamboo leaves, 50-500 parts of coltsfoot flowers, 50-500 parts of sterculia lychnophora, 50-600 parts of liquorice, 50-500 parts of plantain, and 50-500 parts of wolfberry. Or 500-6000 parts of houttuynia, 100-1250 parts of dandelions, 150-1800 parts of honeysuckle, 50-1500 parts of mint, 25-800 parts of American ginseng, 100-2000 parts of lilies, 50-1500 parts of lophatherum gracile, 50-1000 parts of coltsfoot flowers, and 50-500 parts of plantain. Or 500-6000 parts of houttuynia, 25-800 parts of cynanchum chinense, 100-1000 parts of honeysuckle, 100-1250 parts of platycodon, 100-800 parts of plantain, 50-500 parts of American ginseng, 100-1000 parts of wolfberry, 100-800 parts of dandelion, and 50-500 parts of bamboo fungus.

3. The composition of any one of Houttuynia cordata and Chinese medicine according to claim 2, characterized in that: The auxiliary materials also include: white sugar and / or honey.

4. A method for preparing the composition of Houttuynia cordata and traditional Chinese medicine according to any one of claims 1 to 3, characterized in that: The following steps are involved: The herb Houttuynia cordata and its auxiliary materials are respectively soaked in clear water to obtain an extract, and the extracts are mixed and boiled to obtain a composition of Houttuynia cordata and traditional Chinese medicine.

5. Product according to one of the preceding claims, characterized in that It has antioxidant properties.

6. Use of a product according to any one of the preceding claims, characterized in that The composition of Houttuynia cordata and traditional Chinese medicine may further comprise excipients or auxiliary ingredients acceptable in food, medicine, or health food.

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