Agkistrodon halys peptide composite probiotic solid beverage and preparation method thereof

Abstract

The invention discloses an agkistrodon halys peptide composite probiotic solid beverage and a preparation method of the agkistrodon halys peptide composite probiotic solid beverage. Comprising the following raw materials in parts by weight: 12 to 17 parts of ginseng oligopeptide powder, 12 to 17 parts of soybean oligopeptide powder, 5 to 10 parts of agkistrodon halys peptide powder, 10 to 15 parts of ganoderan, 15 to 20 parts of fructo-oligosaccharide, 15 to 20 parts of glucose, 8 to 12 parts of cranberry powder, 0.5 to 1.5 parts of anhydrous citric acid, 5 to 10 parts of bifidobacterium bifidum BGN4, 7 to 13 parts of gamma-aminobutyric acid, 5 to 10 parts of yeast beta-glucan and 0.04 to 0.07 part of vitamin E. According to the solid beverage, various active peptides such as the agkistrodon halys peptide, the ginseng oligopeptide and the soybean oligopeptide are compounded with the probiotics and the dietary fibers, the bioavailability of all the components is improved, absorption of peptide substances can be promoted through the activity of the probiotics, and meanwhile the immunity of the organism can be remarkably enhanced through the synergistic effect of various functional components.

Description

Technical Field

[0001] The invention belongs to the technical field of solid beverages, and particularly relates to a viper peptide compound probiotic solid beverage and a preparation method thereof. Background Art

[0002] With the acceleration of urbanization and the prevalence of unhealthy lifestyles among residents, the number of people suffering from various diseases and sub-health caused by low immunity in my country has increased dramatically. Low immunity causes the body to be unable to play a protective role normally, and is extremely susceptible to infection by bacteria, viruses, fungi, etc. The most direct manifestation is that it is easy to get sick. Frequent illness increases the body's consumption, and symptoms of low immunity such as physical weakness, malnutrition, mental depression, fatigue, decreased appetite, and sleep disorders appear to varying degrees. Getting sick, getting injections, and taking medicine have become commonplace. It takes a long time to recover from each illness, and it often recurs. In the long run, it leads to physical and intellectual stunting, and is also prone to major diseases. Therefore, enhancing immunity and preventing and treating low immunity are important means of preventing diseases.

[0003] Solid beverages are solid products made by adding food additives such as maltodextrin and flavors to food raw materials such as milk, coffee, and tea, and then drying and processing them. Compared with liquid beverages, solid beverages have effectively inhibited the growth of microorganisms after drying, and their shelf life is extended, making them easy to store. Solid beverages on the market are developing in multiple directions, such as enriched nutrition, trendy packaging, and portable.

[0004] Peptides are a class of biologically active substances involved in multiple cell functions in organisms, and participate in regulating various complex physiological processes in the human body. They have the characteristics of high activity, strong affinity and high safety. Micron-level peptide molecules can easily pass through the surface of the skin and directly enter the deep skin layer, and are quickly absorbed by the human body to exert their effects. Modern pharmacological studies have shown that some active peptides not only have antioxidant activity, but can also effectively promote the growth and proliferation of human skin fibroblasts. In particular, they can effectively promote the synthesis of cell matrices, especially collagen and other matrices, by increasing the activity of skin fibroblasts, and play a significant role in anti-skin aging.

[0005] Probiotics can synthesize digestive enzymes, promote the absorption of intestinal nutrients, remove or reduce the adhesion of pathogenic bacteria, maintain the balance of intestinal flora structure, and improve symptoms of constipation, diarrhea, and indigestion. The peptidoglycan, lipoteichoic acid and other ingredients contained in probiotics can improve the body's immunity and enhance the activity of the body's inherent immune cells and natural killer cells.

[0006] At present, the varieties of solid beverages in my country are mainly concentrated in instant tea and fruit juice products, while there are fewer products of probiotic solid beverages. Chinese patent application CN102277405A discloses a method for preparing snake skin active peptides, which uses fresh snake skin as raw material, and is washed, minced, refined, adjusted material concentration, adjusted material temperature, added with protease hydrolysis, centrifugal filtration, enzyme inactivation, nanofiltration desalting concentration, ultrafiltration purification and freeze drying to obtain snake skin active peptides. More than 90% of the product molecular weight is distributed between 200-50000Da, and it also contains ingredients such as DHA and EPA. Although the method uses protease to hydrolyze snake skin collagen, the process is simple, the production cycle is short, and the obtained active peptides have excellent water solubility, stability and moisture absorption and moisturizing functions, but the yield of snake skin active peptides obtained by enzyme cleavage is low, the types of effective small molecule peptides are few, the antioxidant activity is not high, and the obtained product contains more invalid components, the separation process is complicated, and the pertinence is not strong.

[0007] Therefore, it is of great significance to develop a viper peptide composite probiotic solid beverage with immune enhancement function in the art. Summary of the invention

[0008] In view of the deficiencies in the prior art, the object of the present invention is to provide a viper peptide composite probiotic solid beverage and a preparation method thereof. The solid beverage is prepared by compounding a variety of active peptides such as viper peptide, ginseng oligopeptide and soybean oligopeptide with probiotics and dietary fiber, thereby not only improving the bioavailability of each component, but also promoting the absorption of peptide substances through the activity of probiotics. At the same time, the synergistic effect of various functional ingredients can significantly enhance the body's immunity.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A viper peptide compound probiotic solid beverage, comprising the following raw materials by weight:

[0011] 12-17 parts of ginseng oligopeptide powder, 12-17 parts of soybean oligopeptide powder, 5-10 parts of pit viper peptide powder, 10-15 parts of ganoderma lucidum polysaccharide, 15-20 parts of oligofructose, 15-20 parts of glucose, 8-12 parts of cranberry powder, 0.5-1.5 parts of anhydrous citric acid, 5-10 parts of Bifidobacterium bifidum BGN4, 7-13 parts of γ-aminobutyric acid, 5-8 parts of yeast β-glucan, and 0.04-0.07 parts of vitamin E.

[0012] Preferably, the agkistrodon acutus peptide composite probiotic solid beverage comprises the following raw materials by weight:

[0013] 13-15 parts of ginseng oligopeptide powder, 14-16 parts of soybean oligopeptide powder, 7-10 parts of pit viper peptide powder, 12-15 parts of ganoderma lucidum polysaccharide, 15-18 parts of oligofructose, 17-20 parts of glucose, 10-12 parts of cranberry powder, 1-1.5 parts of anhydrous citric acid, 7-10 parts of Bifidobacterium bifidum BGN4, 10-13 parts of γ-aminobutyric acid, 6-7 parts of yeast β-glucan, and 0.05-0.06 parts of vitamin E.

[0014] Preferably, the agkistrodon acutus peptide composite probiotic solid beverage comprises the following raw materials by weight:

[0015] 15 parts of ginseng oligopeptide powder, 14 parts of soybean oligopeptide powder, 8 parts of pit viper peptide powder, 13 parts of ganoderma lucidum polysaccharide, 17 parts of oligofructose, 18 parts of glucose, 11 parts of cranberry powder, 1 part of anhydrous citric acid, 8 parts of Bifidobacterium bifidum BGN4, 12 parts of gamma-aminobutyric acid, 7 parts of yeast beta-glucan, and 0.06 parts of vitamin E.

[0016] In the present invention, ginseng belongs to the perennial herbaceous plant of the genus Panax in the Araliaceae family, has the title of "king of herbs", and is listed as the first of the "three treasures" of Northeast China. The main component of ginseng is ginsenoside, and ginseng also contains many components required by the human body, such as carbohydrates, proteins, amino acids, vitamins and various trace elements. Modern research has shown that ginseng has the effects of regulating the nervous system, improving cardiovascular and cerebrovascular diseases, regulating immunity, anti-tumor, lowering blood sugar and beautifying the skin.

[0017] In the present invention, the agkistrodon is the whole body of the Viperidae animal, with the internal organs removed. Agkistrodon is also known as the earth snake and the grass flying snake. It is a small venomous snake distributed in various parts of my country. In addition to being edible, it has a very high medical value. "Compendium of Materia Medica" records: "Agkistrodon has the effect of dispelling wind and attacking poison", and is often used to treat rheumatic arthralgia, leprosy, scrofula, sores, scabies, hemorrhoids, tumors and other diseases. Since ancient times, the people have used agkistrodon as a tonic, fatigue relief, and physical strength enhancement; in modern times, it is mainly used as a tonic medicine for strengthening, post-illness and weak constitution.

[0018] The pit viper is one of the species on the list of items that are both food and medicine as specified in a 2002 document by the Ministry of Health. This list is a catalogue of Chinese medicinal materials that can be used as medicine and also as ordinary food, drawn up based on the safety, stability and reliability of Chinese medicinal materials.

[0019] In the present invention, Ganoderma lucidum is a fungus of the genus Ganoderma of the Basidiomycetes, which has been known as "immortal grass" and "auspicious grass" since ancient times. It is a precious Chinese medicine for strengthening the body and nourishing the body. It has a long history of medicinal use in my country and has extremely high medicinal value. It is regarded as a precious Chinese medicinal material and a treasure in the treasure house of traditional Chinese medicine. Modern medicine has proved that Ganoderma lucidum contains a variety of physiologically active substances that can regulate and enhance human immunity.

[0020] In the present invention, cranberry, also known as cranberry and cranberry, is a fruit of the genus Vaccinium, mainly grown in acidic peat soil in the cool zone of the northern hemisphere, and is known as one of the three traditional fruits of North America along with Concord grapes and blueberries. Cranberry contains a large amount of phytochemicals such as flavonoids with physiological activity, most of which have antioxidant effects, can resist lipid peroxidation of unsaturated fatty acids in biofilms, protect the structure and function of biofilms, and prevent various degenerative diseases.

[0021] In the present invention, Bifidobacterium is a non-spore-forming, Gram-positive, anaerobic probiotic actinomycete belonging to the phylum Actinomycetes. It was originally isolated from the feces of breast-fed infants. It has the functions of regulating the balance of intestinal flora, improving human immunity, lowering blood lipid cholesterol levels, and promoting the body's digestion and absorption of nutrients.

[0022] Preferably, the method for preparing the agkistrodon acutus peptide powder comprises the following steps:

[0023] The dried agkistrodon meat is added into a citric acid solution for immersion treatment, and after the treatment is completed, it is filtered, dried and crushed to obtain pretreated agkistrodon meat; then the pretreated agkistrodon meat is added into deionized water, and then trypsin, papain, citric acid fatty acid glyceride and proanthocyanidin are added for enzymolysis, and after the enzymolysis is completed, the enzyme is inactivated, and then the enzymolysis solution is ultrafiltered, concentrated and spray-dried to obtain the agkistrodon peptide powder.

[0024] Preferably, the mass concentration of the citric acid is 8-10%, the temperature of the immersion treatment is 30-40° C., and the time is 1-2 h.

[0025] Preferably, the solid-liquid ratio of the pretreated agkistrodon meat to deionized water is 1:8-12, the added amount of trypsin is 2-3% of the mass of the pretreated agkistrodon meat, the added amount of papain is 1-2% of the mass of the pretreated agkistrodon meat, the added amount of citric acid fatty acid glyceride is 0.5-0.8% of the mass of the pretreated agkistrodon meat, and the added amount of proanthocyanidins is 0.3-0.4% of the mass of the pretreated agkistrodon meat; the temperature of the enzymatic hydrolysis is 45-55°C, and the time is 5-7h; the ultrafiltration adopts a membrane ultrafiltration with a pore size of 1500D, the concentration treatment is concentrated to 1 / 4 of the volume at a pressure of 10kpa, the feed temperature of spray drying is 150-180°C, the discharge temperature is 80-90°C, and the atomization pressure is 15-20MPa.

[0026] In the present invention, citric acid solution is first used for pretreatment, which can effectively soften the tissue structure of the pit viper meat and improve the efficiency of subsequent enzymatic hydrolysis. Then, trypsin and papain are used to enzymatically hydrolyze the pit viper meat. At the same time, citric acid fatty acid glyceride added during the enzymatic hydrolysis process can be used as an emulsifier to enhance the contact area between the enzyme and the substrate and improve the enzymatic hydrolysis efficiency. The added proanthocyanidin acts as a reducing agent to prevent protein oxidation, thereby improving the biological activity of pit viper peptide.

[0027] Preferably, the method for preparing the Ganoderma lucidum polysaccharide comprises the following steps:

[0028] Put Ganoderma lucidum into a grinder and grind it to 200 mesh, then add it into deionized water, and then add cellulase, pectinase, succinic acid monoglyceride, and propyl gallate for enzymolysis. After the enzymolysis is completed, the enzyme is inactivated, extracted in a water bath, and then centrifuged. The supernatant is taken and concentrated to 1 / 4 of the volume under reduced pressure, and 4 times the volume of anhydrous ethanol is added for precipitation. The precipitate is filtered and dried to obtain Ganoderma lucidum polysaccharide.

[0029] Preferably, the solid-liquid ratio of the Ganoderma lucidum to deionized water is 1:10-15, the added amount of the cellulase is 2-4% of the mass of the Ganoderma lucidum, the added amount of the pectinase is 1-2% of the mass of the Ganoderma lucidum, the added amount of the succinic acid monoglyceride is 0.6-0.9% of the mass of the Ganoderma lucidum, the added amount of the propyl gallate is 0.3-0.5% of the mass of the Ganoderma lucidum, the temperature of the enzymolysis is 40-50°C, the time is 30-40min, the temperature of the water bath extraction is 70-80°C, the time is 1-2h, and the pressure of the reduced pressure concentration is 10-20kpa.

[0030] In the present invention, a composite enzymatic hydrolysis system of cellulase and pectinase is used to synergistically degrade the cell wall structure of Ganoderma lucidum. At the same time, succinic acid monoglyceride is innovatively introduced as a penetration enhancer during the enzymatic hydrolysis process to improve the tissue penetration of the enzyme solution, and propyl gallate is added as an enzymatic hydrolysis stabilizer to inhibit the activity of polyphenol oxidase in Ganoderma lucidum, effectively preventing oxidative browning of Ganoderma lucidum polysaccharide during the extraction process. This process can not only improve the extraction rate of Ganoderma lucidum polysaccharide, but also make the prepared Ganoderma lucidum polysaccharide have better biological activity.

[0031] The present invention also protects a method for preparing the above-mentioned agkistrodon compound probiotic solid beverage, comprising the following steps:

[0032] Weigh the raw materials according to the formula, mix ginseng oligopeptide powder, soybean oligopeptide powder, pit viper peptide powder, ganoderma lucidum polysaccharide and cranberry powder evenly, then add fructooligosaccharide, glucose, anhydrous citric acid, Bifidobacterium bifidum BGN4, γ-aminobutyric acid, yeast β-glucan and vitamin E, stir evenly to obtain the product.

[0033] The present invention also protects the use of the above-mentioned agkistrodon compound probiotic solid beverage in enhancing immunity.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] (1) The agkistrodon acutus peptide composite probiotic solid beverage provided by the present invention is prepared by compounding animal small molecule peptides, plant small molecule peptides with fungal polysaccharides, cranberry powder and probiotics in a specific ratio, and supplemented with functional small molecule nutrients γ-aminobutyric acid, yeast β-glucan and vitamin E. Ganoderma lucidum polysaccharide can promote the proliferation of T lymphocytes, yeast β-glucan can activate macrophages, and Bifidobacterium bifidum can enhance mucosal immune function by regulating the balance of intestinal flora. The synergistic effect of the three makes the prepared agkistrodon acutus peptide composite probiotic solid beverage have the effect of significantly enhancing immunity.

[0036] (2) The agkistrodon peptide composite probiotic solid beverage provided by the present invention, when preparing the agkistrodon peptide powder, is first pretreated with a citric acid solution, which can effectively soften the tissue structure of the agkistrodon meat and improve the efficiency of subsequent enzymatic hydrolysis. The agkistrodon meat is then enzymatically hydrolyzed using trypsin and papain. At the same time, the citric acid fatty acid glyceride added during the enzymatic hydrolysis process can be used as an emulsifier to enhance the contact area between the enzyme and the substrate and improve the enzymatic hydrolysis efficiency. The added proanthocyanidin acts as a reducing agent to prevent protein oxidation, thereby improving the biological activity of the agkistrodon peptide. This method enables the prepared agkistrodon peptide powder to have a more ideal molecular weight distribution characteristic, thereby improving the efficacy of the agkistrodon peptide powder.

[0037] (3) The agkistrodon acutus peptide composite probiotic solid beverage provided by the present invention has a wide range of pharmacological activities of Ganoderma lucidum polysaccharide, which can improve the body's immunity and the body's tolerance to hypoxia, eliminate free radicals, inhibit tumors, resist radiation, and improve the liver, bone marrow, and blood The ability to synthesize DNA, RNA and protein; when preparing Ganoderma lucidum polysaccharide, a composite enzymatic hydrolysis system of cellulase and pectinase is used to synergistically degrade the cell wall structure of Ganoderma lucidum, and at the same time, succinic acid monoglyceride is innovatively introduced as a penetration enhancer during the enzymatic hydrolysis process to improve the tissue penetration of the enzyme solution, and propyl gallate is added as an enzymatic hydrolysis stabilizer to inhibit the activity of polyphenol oxidase in Ganoderma lucidum, effectively preventing the oxidative browning of Ganoderma lucidum polysaccharide during the extraction process. This process can not only improve the extraction rate of Ganoderma lucidum polysaccharide, but also make the prepared Ganoderma lucidum polysaccharide have better biological activity. DETAILED DESCRIPTION

[0038] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solution of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] In the present invention, the ginseng oligopeptide powder was purchased from Mingfu (Shanghai) Health Technology Co., Ltd., with a molecular weight between 180-1000 Daltons; the soybean oligopeptide powder was purchased from Mingfu (Shanghai) Health Technology Co., Ltd., with a molecular weight of 500 Daltons; the cranberry powder was purchased from Tianjin Zhenru Food Industry Co., Ltd., which is a water-soluble concentrated powder with a specification of 10:1; the Bifidobacterium bifidum BGN4 was purchased from BIFIDO Company of South Korea.

[0040] Example 1

[0041] A viper peptide compound probiotic solid beverage, comprising the following raw materials by weight:

[0042] 15 parts of ginseng oligopeptide powder, 14 parts of soybean oligopeptide powder, 8 parts of pit viper peptide powder, 13 parts of ganoderma lucidum polysaccharide, 17 parts of oligofructose, 18 parts of glucose, 11 parts of cranberry powder, 1 part of anhydrous citric acid, 8 parts of Bifidobacterium bifidum BGN4, 12 parts of gamma-aminobutyric acid, 7 parts of yeast beta-glucan, and 0.06 parts of vitamin E.

[0043] The preparation method of the agkistrodon acutus peptide powder comprises the following steps:

[0044] The dried agkistrodon meat is added to a citric acid solution with a mass concentration of 9%, and immersed at 35°C for 1.5 hours. After the treatment, it is filtered, dried, crushed, and sieved through an 80-mesh sieve to obtain pretreated agkistrodon meat; then 100g of the pretreated agkistrodon meat is added to 1000g of deionized water, followed by adding 2.5g of trypsin, 1.5g of papain, 0.7g of citric acid fatty acid glyceride, and 0.35g of proanthocyanidin, and enzymolysis is performed at 50°C for 6h. After the enzymolysis is completed, the enzyme is inactivated in boiling water for 10min, and then the enzymolysis solution is ultrafiltered with an ultrafiltration membrane with a pore size of 1500D, concentrated to 1 / 4 of the volume at a pressure of 10kpa, and then spray-dried. The feed temperature of the spray drying is 160°C, the discharge temperature is 85°C, and the atomization pressure is 18Mpa, so as to obtain the agkistrodon peptide powder.

[0045] The preparation method of the ganoderma lucidum polysaccharide comprises the following steps:

[0046] Put 100g of Ganoderma lucidum into a grinder and grind it to 200 mesh, then add it into 1300g of deionized water, followed by adding 3g of cellulase, 1.5g of pectinase, 0.8g of succinic acid monoglyceride, and 0.4g of propyl gallate, and perform enzymolysis at 45℃ for 35min. After the enzymolysis is completed, inactivate the enzyme in boiling water for 10min, extract in a water bath at 70-80℃ for 1.5h, then centrifuge, take the supernatant, and concentrate it to 1 / 4 of the volume under reduced pressure at 15kpa, add 4 times the volume of anhydrous ethanol for precipitation, filter, and dry the precipitate to obtain Ganoderma lucidum polysaccharide.

[0047] A method for preparing a viper peptide compound probiotic solid beverage comprises the following steps:

[0048] Weigh the raw materials according to the formula, mix ginseng oligopeptide powder, soybean oligopeptide powder, pit viper peptide powder, ganoderma lucidum polysaccharide and cranberry powder evenly, then add fructooligosaccharide, glucose, anhydrous citric acid, Bifidobacterium bifidum BGN4, γ-aminobutyric acid, yeast β-glucan and vitamin E, stir evenly to obtain the product.

[0049] Example 2

[0050] A viper peptide compound probiotic solid beverage, comprising the following raw materials by weight:

[0051] 14 parts of ginseng oligopeptide powder, 15 parts of soybean oligopeptide powder, 7 parts of pit viper peptide powder, 12 parts of ganoderma lucidum polysaccharide, 12 parts of oligofructose, 13 parts of glucose, 10 parts of cranberry powder, 1 part of anhydrous citric acid, 7 parts of Bifidobacterium bifidum BGN4, 9 parts of gamma-aminobutyric acid, 6 parts of yeast beta-glucan, and 0.05 parts of vitamin E.

[0052] The preparation method of the agkistrodon acutus peptide powder comprises the following steps:

[0053] The dried agkistrodon meat is added to a citric acid solution with a mass concentration of 9%, and immersed at 35°C for 1.5 hours. After the treatment, it is filtered, dried, crushed, and passed through an 80-mesh sieve to obtain pretreated agkistrodon meat; then 100g of the pretreated agkistrodon meat is added to 900g of deionized water, followed by adding 2.5g of trypsin, 1.5g of papain, 0.6g of citric acid fatty acid glyceride, and 0.35g of proanthocyanidin, and enzymolysis is performed at 50°C for 6h. After the enzymolysis is completed, the enzyme is inactivated in boiling water for 10min, and then the enzymolysis solution is ultrafiltered with an ultrafiltration membrane with a pore size of 1500D, concentrated to 1 / 4 of the volume at a pressure of 10kpa, and then spray-dried. The feed temperature of the spray drying is 170°C, the discharge temperature is 85°C, and the atomization pressure is 17Mpa, so as to obtain the agkistrodon peptide powder.

[0054] The preparation method of the ganoderma lucidum polysaccharide comprises the following steps:

[0055] 100g of Ganoderma lucidum was put into a grinder and crushed to 200 mesh, then added into 1200g of deionized water, followed by 3g of cellulase, 1.5g of pectinase, 0.7g of succinic acid monoglyceride, and 0.4g of propyl gallate, and enzymolysis was performed at 45°C for 35min. After the enzymolysis was completed, the enzyme was inactivated in boiling water for 10min, and extracted in a water bath at 75°C for 1.5h, followed by centrifugation. The supernatant was taken and concentrated to 1 / 4 of the volume under reduced pressure at 15kpa, and 4 times the volume of anhydrous ethanol was added for precipitation. The precipitate was filtered and dried to obtain Ganoderma lucidum polysaccharide.

[0056] A method for preparing a viper peptide compound probiotic solid beverage comprises the following steps:

[0057] Weigh the raw materials according to the formula, mix ginseng oligopeptide powder, soybean oligopeptide powder, pit viper peptide powder, ganoderma lucidum polysaccharide and cranberry powder evenly, then add fructooligosaccharide, glucose, anhydrous citric acid, Bifidobacterium bifidum BGN4, γ-aminobutyric acid, yeast β-glucan and vitamin E, stir evenly to obtain the product.

[0058] Example 3

[0059] A viper peptide compound probiotic solid beverage, comprising the following raw materials by weight:

[0060] 12 parts of ginseng oligopeptide powder, 12 parts of soybean oligopeptide powder, 5 parts of pit viper peptide powder, 10 parts of ganoderma lucidum polysaccharide, 15 parts of oligofructose, 15 parts of glucose, 8 parts of cranberry powder, 0.5 parts of anhydrous citric acid, 5 parts of Bifidobacterium bifidum BGN4, 7 parts of γ-aminobutyric acid, 5 parts of yeast β-glucan, and 0.04 parts of vitamin E.

[0061] The preparation method of the agkistrodon acutus peptide powder comprises the following steps:

[0062] The dried agkistrodon meat is added to a citric acid solution with a mass concentration of 8%, and immersed at 30°C for 2h. After the treatment, it is filtered, dried, crushed, and sieved through an 80-mesh sieve to obtain pretreated agkistrodon meat; then 100g of the pretreated agkistrodon meat is added to 800g of deionized water, followed by adding 2g of trypsin, 1g of papain, 0.5g of citric acid fatty acid glyceride, and 0.3g of proanthocyanidin, and enzymolysis is performed at 45°C for 7h. After the enzymolysis is completed, the enzyme is inactivated in boiling water for 10min, and then the enzymolysis solution is ultrafiltered with an ultrafiltration membrane with a pore size of 1500D, concentrated to 1 / 4 of the volume at a pressure of 10kpa, and then spray-dried. The feed temperature of the spray drying is 150°C, the discharge temperature is 80°C, and the atomization pressure is 15Mpa, so as to obtain the agkistrodon peptide powder.

[0063] The preparation method of the ganoderma lucidum polysaccharide comprises the following steps:

[0064] Put 100g of Ganoderma lucidum into a grinder and grind it to 200 mesh, then add it into 1000g of deionized water, followed by adding 2g of cellulase, 1g of pectinase, 0.6g of succinic acid monoglyceride, and 0.3g of propyl gallate, and perform enzymolysis at 40℃ for 40min. After the enzymolysis is completed, inactivate the enzyme in boiling water for 10min, extract it in a water bath at 70℃ for 2h, then centrifuge it, take the supernatant, and concentrate it to 1 / 4 of its volume under reduced pressure at a pressure of 10kPa, add 4 times the volume of anhydrous ethanol for precipitation, filter it, and dry the precipitate to obtain Ganoderma lucidum polysaccharide.

[0065] A method for preparing a viper peptide compound probiotic solid beverage comprises the following steps:

[0066] Weigh the raw materials according to the formula, mix ginseng oligopeptide powder, soybean oligopeptide powder, pit viper peptide powder, ganoderma lucidum polysaccharide and cranberry powder evenly, then add fructooligosaccharide, glucose, anhydrous citric acid, Bifidobacterium bifidum BGN4, γ-aminobutyric acid, yeast β-glucan and vitamin E, stir evenly to obtain the product.

[0067] Example 4

[0068] A viper peptide compound probiotic solid beverage, comprising the following raw materials by weight:

[0069] 17 parts of ginseng oligopeptide powder, 17 parts of soybean oligopeptide powder, 10 parts of agkistrodon powder, 15 parts of ganoderma lucidum polysaccharide, 200 parts of oligofructose, 20 parts of glucose, 12 parts of cranberry powder, 1.5 parts of anhydrous citric acid, 10 parts of Bifidobacterium bifidum BGN4, 13 parts of gamma-aminobutyric acid, 8 parts of yeast beta-glucan, and 0.07 parts of vitamin E.

[0070] The preparation method of the agkistrodon acutus peptide powder comprises the following steps:

[0071] The dried agkistrodon meat is added to a citric acid solution with a mass concentration of 10%, and immersed at 40°C for 1 hour. After the treatment, it is filtered, dried, crushed, and sieved through an 80-mesh sieve to obtain pretreated agkistrodon meat; then 100g of the pretreated agkistrodon meat is added to 1200g of deionized water, followed by adding 3g of trypsin, 2g of papain, 0.8g of citric acid fatty acid glyceride, and 0.4g of proanthocyanidin, and enzymolysis is performed at 55°C for 5h. After the enzymolysis is completed, the enzyme is inactivated in boiling water for 10min, and then the enzymolysis solution is ultrafiltered with an ultrafiltration membrane with a pore size of 1500D, concentrated to 1 / 4 of the volume at a pressure of 10kpa, and then spray-dried. The feed temperature of the spray drying is 180°C, the discharge temperature is 90°C, and the atomization pressure is 20Mpa, so as to obtain the agkistrodon peptide powder.

[0072] The preparation method of the ganoderma lucidum polysaccharide comprises the following steps:

[0073] 100g of Ganoderma lucidum was put into a grinder and crushed to 200 mesh, then added into 1500g of deionized water, followed by 4g of cellulase, 2g of pectinase, 0.9g of succinic acid monoglyceride, and 0.5g of propyl gallate, and enzymolysis was performed at 50°C for 30min. After the enzymolysis was completed, the enzyme was inactivated in boiling water for 10min, and extracted in a water bath at 80°C for 1h, followed by centrifugation, and the supernatant was taken and concentrated to 1 / 4 of the volume under reduced pressure at 20kPa, and 4 times the volume of anhydrous ethanol was added for precipitation, filtered, and the precipitate was dried to obtain Ganoderma lucidum polysaccharide.

[0074] A method for preparing a viper peptide compound probiotic solid beverage comprises the following steps:

[0075] Weigh the raw materials according to the formula, mix ginseng oligopeptide powder, soybean oligopeptide powder, pit viper peptide powder, ganoderma lucidum polysaccharide and cranberry powder evenly, then add fructooligosaccharide, glucose, anhydrous citric acid, Bifidobacterium bifidum BGN4, γ-aminobutyric acid, yeast β-glucan and vitamin E, stir evenly to obtain the product.

[0076] Comparative Example 1

[0077] A viper peptide compound probiotic solid beverage, comprising the following raw materials by weight:

[0078] 15 parts of ginseng oligopeptide powder, 14 parts of soybean oligopeptide powder, 8 parts of pit viper peptide powder, 13 parts of ganoderma lucidum polysaccharide, 17 parts of oligofructose, 18 parts of glucose, 11 parts of cranberry powder, 1 part of anhydrous citric acid, 8 parts of Bifidobacterium bifidum BGN4, 12 parts of gamma-aminobutyric acid, 7 parts of yeast beta-glucan, and 0.06 parts of vitamin E.

[0079] The preparation method of the agkistrodon acutus peptide powder comprises the following steps:

[0080] The dried agkistrodon meat is added to a citric acid solution with a mass concentration of 9%, and immersed at 35°C for 1.5 hours. After the treatment, it is filtered, dried, crushed, and sieved through an 80-mesh sieve to obtain pretreated agkistrodon meat; then 100g of the pretreated agkistrodon meat is added to 1000g of deionized water, followed by adding 2.5g of trypsin and 1.5g of papain, and enzymolysis is performed at 50°C for 6h. After the enzymolysis is completed, the enzyme is inactivated in boiling water for 10min, and then the enzymolysis solution is ultrafiltered with an ultrafiltration membrane with a pore size of 1500D, concentrated to 1 / 4 of the volume at a pressure of 10kpa, and then spray-dried. The feed temperature of the spray drying is 160°C, the discharge temperature is 85°C, and the atomization pressure is 18Mpa, so as to obtain the agkistrodon peptide powder.

[0081] The preparation method of the ganoderma lucidum polysaccharide comprises the following steps:

[0082] Put 100g of Ganoderma lucidum into a grinder and grind it to 200 mesh, then add it into 1300g of deionized water, followed by adding 3g of cellulase, 1.5g of pectinase, 0.8g of succinic acid monoglyceride, and 0.4g of propyl gallate, and perform enzymolysis at 45℃ for 35min. After the enzymolysis is completed, inactivate the enzyme in boiling water for 10min, extract in a water bath at 70-80℃ for 1.5h, then centrifuge, take the supernatant, and concentrate it to 1 / 4 of the volume under reduced pressure at 15kpa, add 4 times the volume of anhydrous ethanol for precipitation, filter, and dry the precipitate to obtain Ganoderma lucidum polysaccharide.

[0083] A method for preparing a viper peptide compound probiotic solid beverage comprises the following steps:

[0084] Weigh the raw materials according to the formula, mix ginseng oligopeptide powder, soybean oligopeptide powder, pit viper peptide powder, ganoderma lucidum polysaccharide and cranberry powder evenly, then add fructooligosaccharide, glucose, anhydrous citric acid, Bifidobacterium bifidum BGN4, γ-aminobutyric acid, yeast β-glucan and vitamin E, stir evenly to obtain the product.

[0085] Compared with Example 1, in this comparative example, no citric acid fatty acid glyceride and proanthocyanidin were added when preparing the agkistrodon peptide powder.

[0086] Comparative Example 2

[0087] A viper peptide compound probiotic solid beverage, comprising the following raw materials by weight:

[0088] 15 parts of ginseng oligopeptide powder, 14 parts of soybean oligopeptide powder, 8 parts of pit viper peptide powder, 13 parts of ganoderma lucidum polysaccharide, 17 parts of oligofructose, 18 parts of glucose, 11 parts of cranberry powder, 1 part of anhydrous citric acid, 8 parts of Bifidobacterium bifidum BGN4, 12 parts of gamma-aminobutyric acid, 7 parts of yeast beta-glucan, and 0.06 parts of vitamin E.

[0089] The preparation method of the agkistrodon acutus peptide powder comprises the following steps:

[0090] The dried agkistrodon meat is added to a citric acid solution with a mass concentration of 9%, and immersed at 35°C for 1.5 hours. After the treatment, it is filtered, dried, crushed, and sieved through an 80-mesh sieve to obtain pretreated agkistrodon meat; then 100g of the pretreated agkistrodon meat is added to 1000g of deionized water, followed by adding 2.5g of trypsin, 1.5g of papain, 0.7g of citric acid fatty acid glyceride, and 0.35g of proanthocyanidin, and enzymolysis is performed at 50°C for 6h. After the enzymolysis is completed, the enzyme is inactivated in boiling water for 10min, and then the enzymolysis solution is ultrafiltered with an ultrafiltration membrane with a pore size of 1500D, concentrated to 1 / 4 of the volume at a pressure of 10kpa, and then spray-dried. The feed temperature of the spray drying is 160°C, the discharge temperature is 85°C, and the atomization pressure is 18Mpa, so as to obtain the agkistrodon peptide powder.

[0091] The preparation method of the ganoderma lucidum polysaccharide comprises the following steps:

[0092] 100g of Ganoderma lucidum was put into a grinder and crushed to 200 mesh, then added into 1300g of deionized water, followed by 3g of cellulase and 1.5g of pectinase, and enzymolysis was performed at 45°C for 35min. After enzymolysis, the enzyme was inactivated in boiling water for 10min, and extracted in a water bath at 70-80°C for 1.5h, then centrifuged, the supernatant was taken, and concentrated to 1 / 4 of the volume under reduced pressure at 15kpa, and 4 times the volume of anhydrous ethanol was added for precipitation, filtered, and the precipitate was dried to obtain Ganoderma lucidum polysaccharide.

[0093] A method for preparing a viper peptide compound probiotic solid beverage comprises the following steps:

[0094] Weigh the raw materials according to the formula, mix ginseng oligopeptide powder, soybean oligopeptide powder, pit viper peptide powder, ganoderma lucidum polysaccharide and cranberry powder evenly, then add fructooligosaccharide, glucose, anhydrous citric acid, Bifidobacterium bifidum BGN4, γ-aminobutyric acid, yeast β-glucan and vitamin E, stir evenly to obtain the product.

[0095] Compared with Example 1, in this comparative example, no succinic acid monoglyceride and propyl gallate were added when preparing Ganoderma lucidum polysaccharide.

[0096] Comparative Example 3

[0097] A viper peptide compound probiotic solid beverage, comprising the following raw materials by weight:

[0098] 14 parts of soybean oligopeptide powder, 8 parts of pit viper peptide powder, 13 parts of ganoderma lucidum polysaccharide, 17 parts of oligofructose, 18 parts of glucose, 11 parts of cranberry powder, 1 part of anhydrous citric acid, 12 parts of gamma-aminobutyric acid, 7 parts of yeast beta-glucan, and 0.06 parts of vitamin E.

[0099] The preparation method of the agkistrodon acutus peptide powder comprises the following steps:

[0100] The dried agkistrodon meat is added to a citric acid solution with a mass concentration of 9%, and immersed at 35°C for 1.5 hours. After the treatment, it is filtered, dried, crushed, and sieved through an 80-mesh sieve to obtain pretreated agkistrodon meat; then 100g of the pretreated agkistrodon meat is added to 1000g of deionized water, followed by adding 2.5g of trypsin, 1.5g of papain, 0.7g of citric acid fatty acid glyceride, and 0.35g of proanthocyanidin, and enzymolysis is performed at 50°C for 6h. After the enzymolysis is completed, the enzyme is inactivated in boiling water for 10min, and then the enzymolysis solution is ultrafiltered with an ultrafiltration membrane with a pore size of 1500D, concentrated to 1 / 4 of the volume at a pressure of 10kpa, and then spray-dried. The feed temperature of the spray drying is 160°C, the discharge temperature is 85°C, and the atomization pressure is 18Mpa, so as to obtain the agkistrodon peptide powder.

[0101] The preparation method of the ganoderma lucidum polysaccharide comprises the following steps:

[0102] Put 100g of Ganoderma lucidum into a grinder and grind it to 200 mesh, then add it into 1300g of deionized water, followed by adding 3g of cellulase, 1.5g of pectinase, 0.8g of succinic acid monoglyceride, and 0.4g of propyl gallate, and perform enzymolysis at 45℃ for 35min. After the enzymolysis is completed, inactivate the enzyme in boiling water for 10min, extract in a water bath at 70-80℃ for 1.5h, then centrifuge, take the supernatant, and concentrate it to 1 / 4 of the volume under reduced pressure at 15kpa, add 4 times the volume of anhydrous ethanol for precipitation, filter, and dry the precipitate to obtain Ganoderma lucidum polysaccharide.

[0103] A method for preparing a viper peptide compound probiotic solid beverage comprises the following steps:

[0104] Weigh the raw materials according to the formula, mix the soybean oligopeptide powder, pit viper peptide powder, ganoderma lucidum polysaccharide and cranberry powder evenly, then add oligofructose, glucose, anhydrous citric acid, γ-aminobutyric acid, yeast β-glucan and vitamin E, stir evenly to obtain the product.

[0105] Compared with Example 1, no ginseng oligopeptide powder and Bifidobacterium bifidum BGN4 were added in this comparative example.

[0106] The acerpeptide compound probiotic solid beverage prepared in Examples 1-4 and Comparative Examples 1-3 was subjected to a mouse experiment to verify that the acerpeptide compound probiotic solid beverage prepared in the present invention is helpful in enhancing immunity, as follows:

[0107] 180 SPF Kunming male mice were randomly divided into 9 groups, 20 mice in each group. The mice were 6-8 weeks old and weighed 18-22 g. The grouping is shown in Table 1. The experimental animals were kept in a barrier environment animal room with a temperature of 22°C, a humidity of 40-70%, and a 12-h light-dark cycle. They were fed with standard feed and adaptively fed for 1 week with free access to water. The blank group and the model group were gavaged with normal saline for 30 consecutive days; the Example 1 group was gavaged with the solid beverage in Example 1, the Example 2 group was gavaged with the solid beverage in Example 2, the Example 3 group was gavaged with the solid beverage in Example 3, the Example 4 group was gavaged with the solid beverage in Example 4, the Comparative Example 1 group was gavaged with the solid beverage in Comparative Example 1, the Comparative Example 2 group was gavaged with the solid beverage in Comparative Example 2, and the Comparative Example 3 group was gavaged with the solid beverage in Comparative Example 3, with a gavage dose of 200 mg / kg / day (5 times the recommended amount for humans) for 30 consecutive days; on the 1st to 3rd day, except for the blank group, the other groups of mice were intraperitoneally injected with normal saline, and 100 μL cyclophosphamide (40 mg / kg) was injected intraperitoneally to establish an immunocompromised mouse model. 12 hours after the last gavage of the mice, the weight was recorded and the mice were killed by cervical dislocation, and the thymus and spleen were aseptically removed and the weight was recorded.

[0108] Table 1 Experimental grouping

[0109]

[0110]

[0111] (1) Spleen lymphocyte transformation experiment: On the 31st day of the experiment, 5 mice were randomly selected from each group, their weights were recorded, and the mice were killed by cervical dislocation. The thymus and spleen were removed aseptically and their weights were recorded. Subsequently, the mouse spleen lymphocyte transformation experiment was determined by the MTT method, and the NK cell activity was determined by the lactate dehydrogenase (LDH) method. The results are shown in Table 2:

[0112] Table 2 Lymphatic organ / body weight ratio, optical density difference, and NK cell activity test results of each group of mice

[0113]

[0114] Note: Compared with the blank group, *P<0.05, **P<0.01; compared with the model group, #P<0.05, ##P<0.01

[0115] As can be seen from Table 2, the solid beverage prepared by the present invention can significantly improve the thymus / body weight ratio and spleen / body weight ratio of mice, and can also enhance the conA-induced mouse spleen lymphocyte transformation and mouse NK cell activity. The solid beverage prepared in Example 1 has better efficacy than those of Comparative Examples 1-3, indicating that there is a synergistic effect between the substances in the raw materials.

[0116] (2) Delayed hypersensitivity reaction experiment: On the 31st day of the experiment, 5 mice were randomly selected from each group and each mouse was intraperitoneally injected with 0.2 mL of 2% (v / v) packed sheep red blood cell (SRBC) suspension for immunization. 4 days after immunization, the thickness of the left hind foot plantar was measured, and then 20% (v / v) SRBC was subcutaneously injected at the measurement site, 20 μL (about 1×10 8 SRBC), the thickness of the left hind foot plantar was measured 24 hours after injection, and the same part was measured three times and the average value was taken. The results are shown in Table 3:

[0117] Table 3 Test results of thickness difference of left hind foot of mice in each group

[0118] Group Number of animals Thickness difference of left hindfoot plantar part (mm) Blank group 5 0.96±0.23 Model Group 5 0.57±0.11** Example 1 Group 5 <![CDATA[0.87±0.21 ## ]]> Example 2 Group 5 <![CDATA[0.83±0.17 ## ]]> Example 3 Group 5 <![CDATA[0.79±0.19 ## ]]> Example 4 Group 5 <![CDATA[0.84±0.18 ## ]]> Comparative Example 1 5 <![CDATA[0.69±0.14 # ]]> Comparative Example 2 5 <![CDATA[0.71±0.13 # ]]> Comparative Example 4 5 <![CDATA[0.65±0.15 # ]]>

[0119] Note: Compared with the blank group, *P<0.05, **P<0.01; compared with the model group, #P<0.05, ##P<0.01

[0120] As can be seen from Table 3, compared with the blank group, the thickness difference of the left hind foot plantar of the model group was significantly decreased, and the difference was extremely significant, indicating that the immunosuppressed mouse model was successfully established, while the thickness difference of the left hind foot plantar of the mice in Example 1-4 groups was significantly increased compared with the model group, and the difference was extremely significant, indicating that the solid beverage prepared by the present invention has an enhancing effect on delayed-type hypersensitivity in mice.

[0121] (3) Humoral immune function determination: On the 31st day of the experiment, 5 mice were randomly selected from each group, and each mouse was immunized by intraperitoneal injection of 0.2 mL of 2% (v / v) SRBC suspension. After 4 days, the mice were killed by cervical dislocation. The serum hemolysin level was determined by hemagglutination method; the ability of mice to produce antibodies to SRBC was determined by Jerne's modified slide method. The results are shown in Table 4:

[0122] Table 4 Results of humoral immunity test of mice in each group

[0123] Group Number of animals Number of hemolytic plaques Anti-volume number Blank group 5 36.9±6.5 121±26 Model Group 5 25.4±4.1** 72±15** Example 1 Group 5 <![CDATA[34.8±5.9 ## ]]> <![CDATA[103±27 ## ]]> Example 2 Group 5 <![CDATA[34.2±5.5 ## ]]> <![CDATA[97±20 ## ]]> Example 3 Group 5 <![CDATA[33.9±4.6 ## ]]> <![CDATA[95±23 ## ]]> Example 4 Group 5 <![CDATA[34.5±5.3 ## ]]> <![CDATA[98±21 ## ]]> Comparative Example 1 5 <![CDATA[30.7±3.5 # ]]> <![CDATA[87±18 # ]]> Comparative Example 2 5 <![CDATA[31.6±4.4 # ]]> <![CDATA[88±20 # ]]> Comparative Example 3 5 29.1±3.9 <![CDATA[84±17 # ]]>

[0124] Note: Compared with the blank group, *P<0.05, **P<0.01; compared with the model group, #P<0.05, ##P<0.01

[0125] It can be seen from Table 4 that the solid beverage prepared by the present invention can significantly improve the humoral immune function of mice, and the efficacy of the solid beverage prepared in Example 1 is better than that of the comparative examples 1-3, indicating that there is a synergistic effect between the substances in the raw materials.

[0126] (4) Peritoneal macrophage phagocytosis experiment: On the 31st day of the experiment, 5 mice were randomly selected from each group and 1 mL of 20% (v / v) chicken red blood cell suspension was injected into the peritoneal cavity of each mouse. The mice were killed by cervical dislocation at intervals of 1 h. Peritoneal fluid droplets were collected and incubated in a 38°C incubator for 30 min. The cells were fixed, stained, and examined under a microscope. 100 macrophages were counted, and the phagocytic rate and phagocytic index were calculated.

[0127]

[0128] The results are shown in Table 5:

[0129] Table 5 Test results of phagocytosis rate and phagocytosis index of mice in each group

[0130] Group Number of animals Phagocytosis rate (%) Phagocytic index Blank group 5 25.1±3.6 0.54±0.16 Model Group 5 16.2±2.1** 0.31±0.08** Example 1 Group 5 <![CDATA[24.6±3.2 ## ]]> <![CDATA[0.49±0.17 ## ]]> Example 2 Group 5 <![CDATA[24.1±3.5 ## ]]> <![CDATA[0.46±0.15 ## ]]> Example 3 Group 5 <![CDATA[23.9±2.6 ## ]]> <![CDATA[0.44±0.12 ## ]]> Example 4 Group 5 <![CDATA[24.4±3.3 ## ]]> <![CDATA[0.47±0.13 ## ]]> Comparative Example 1 5 <![CDATA[21.1±3.1 # ]]> <![CDATA[0.41±0.11 # ]]> Comparative Example 2 5 <![CDATA[21.8±2.9 # ]]> <![CDATA[0.42±0.12 # ]]> Comparative Example 3 5 <![CDATA[20.1±2.5 # ]]> <![CDATA[0.39±0.10 # ]]>

[0131] Note: Compared with the blank group, *P<0.05, **P<0.01; compared with the model group, #P<0.05, ##P<0.01

[0132] As can be seen from Table 5, compared with the blank group, the phagocytic rate and phagocytic index of the mice in the model group were significantly decreased, indicating that the immunosuppressed mice model was successfully established, while the phagocytic rate and phagocytic index of the mice in the Example 1-4 groups were significantly increased compared with the model group, and the difference was extremely significant, indicating that the solid beverage prepared by the present invention has an effect of increasing the ability of mouse macrophages to phagocytose chicken red blood cells, and can improve the immunity of mice.

[0133] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A viper peptide compound probiotic solid beverage, characterized in that: By weight, it includes the following raw materials: 12-17 parts of ginseng oligopeptide powder, 12-17 parts of soybean oligopeptide powder, 5-10 parts of pit viper peptide powder, 10-15 parts of ganoderma lucidum polysaccharide, 15-20 parts of oligofructose, 15-20 parts of glucose, 8-12 parts of cranberry powder, 0.5-1.5 parts of anhydrous citric acid, 5-10 parts of Bifidobacterium bifidum BGN4, 7-13 parts of γ-aminobutyric acid, 5-8 parts of yeast β-glucan, and 0.04-0.07 parts of vitamin E.

2. The agkistrodon compound probiotic solid beverage according to claim 1, characterized in that: By weight, it includes the following raw materials: 13-15 parts of ginseng oligopeptide powder, 14-16 parts of soybean oligopeptide powder, 7-10 parts of pit viper peptide powder, 12-15 parts of ganoderma lucidum polysaccharide, 15-18 parts of oligofructose, 17-20 parts of glucose, 10-12 parts of cranberry powder, 1-1.5 parts of anhydrous citric acid, 7-10 parts of Bifidobacterium bifidum BGN4, 10-13 parts of γ-aminobutyric acid, 6-7 parts of yeast β-glucan, and 0.05-0.06 parts of vitamin E.

3. The agkistrodon compound probiotic solid beverage according to claim 1, characterized in that: By weight, it includes the following raw materials: 15 parts of ginseng oligopeptide powder, 14 parts of soybean oligopeptide powder, 8 parts of pit viper peptide powder, 13 parts of ganoderma lucidum polysaccharide, 17 parts of oligofructose, 18 parts of glucose, 11 parts of cranberry powder, 1 part of anhydrous citric acid, 8 parts of Bifidobacterium bifidum BGN4, 12 parts of gamma-aminobutyric acid, 7 parts of yeast beta-glucan, and 0.06 parts of vitamin E.

4. The agkistrodon compound probiotic solid beverage according to claim 1, characterized in that: The preparation method of the agkistrodon acutus peptide powder comprises the following steps: The dried agkistrodon meat is added into a citric acid solution for immersion treatment, and after the treatment is completed, it is filtered, dried and crushed to obtain pretreated agkistrodon meat; then the pretreated agkistrodon meat is added into deionized water, and then trypsin, papain, citric acid fatty acid glyceride and proanthocyanidin are added for enzymolysis, and after the enzymolysis is completed, the enzyme is inactivated, and then the enzymolysis solution is ultrafiltered, concentrated and spray-dried to obtain the agkistrodon peptide powder.

5. The agkistrodon compound probiotic solid beverage according to claim 3, characterized in that: The mass concentration of the citric acid is 8-10%, the temperature of the immersion treatment is 30-40° C., and the time is 1-2 hours.

6. The agkistrodon compound probiotic solid beverage according to claim 3, characterized in that: The material-liquid ratio of the pretreated agkistrodon meat to deionized water is 1:8-12, the added amount of trypsin is 2-3% of the mass of the pretreated agkistrodon meat, the added amount of papain is 1-2% of the mass of the pretreated agkistrodon meat, the added amount of citric acid fatty acid glyceride is 0.5-0.8% of the mass of the pretreated agkistrodon meat, and the added amount of proanthocyanidins is 0.3-0.4% of the mass of the pretreated agkistrodon meat; the temperature of the enzymolysis is 45-55°C, and the time is 5-7h; the ultrafiltration adopts a membrane ultrafiltration with a pore size of 1500D, the concentration treatment is concentrated to 1 / 4 of the volume at a pressure of 10kpa, the feed temperature of the spray drying is 150-180°C, the discharge temperature is 80-90°C, and the atomization pressure is 15-20MPa.

7. The agkistrodon compound probiotic solid beverage according to claim 1, characterized in that: The preparation method of the ganoderma lucidum polysaccharide comprises the following steps: Put Ganoderma lucidum into a grinder and grind it to 200 mesh, then add it into deionized water, and then add cellulase, pectinase, succinic acid monoglyceride, and propyl gallate for enzymolysis. After the enzymolysis is completed, the enzyme is inactivated, extracted in a water bath, and then centrifuged. The supernatant is taken and concentrated to 1 / 4 of the volume under reduced pressure, and 4 times the volume of anhydrous ethanol is added for precipitation. The precipitate is filtered and dried to obtain Ganoderma lucidum polysaccharide.

8. The agkistrodon compound probiotic solid beverage according to claim 7, characterized in that: The solid-liquid ratio of the ganoderma lucidum to deionized water is 1:10-15, the added amount of the cellulase is 2-4% of the mass of the ganoderma lucidum, the added amount of the pectinase is 1-2% of the mass of the ganoderma lucidum, the added amount of the succinic acid monoglyceride is 0.6-0.9% of the mass of the ganoderma lucidum, the added amount of the propyl gallate is 0.3-0.5% of the mass of the ganoderma lucidum, the temperature of the enzymolysis is 40-50°C, the time is 30-40min, the temperature of the water bath extraction is 70-80°C, the time is 1-2h, and the pressure of the reduced pressure concentration is 10-20kpa.

9. A method for preparing the agkistrodon compound probiotic solid beverage according to any one of claims 1 to 8, characterized in that: The following steps are involved: Weigh the raw materials according to the formula, mix ginseng oligopeptide powder, soybean oligopeptide powder, pit viper peptide powder, ganoderma lucidum polysaccharide and cranberry powder evenly, then add fructooligosaccharide, glucose, anhydrous citric acid, Bifidobacterium bifidum BGN4, γ-aminobutyric acid, yeast β-glucan and vitamin E, stir evenly to obtain the product.

10. Use of the acerpeptide compound probiotic solid beverage according to any one of claims 1 to 8 in enhancing immunity.

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