Hangover alleviating composition containing dendrobium small molecule peptide and preparation method of hangover alleviating composition

Abstract

The invention relates to the technical field of biology, in particular to a hangover alleviating composition containing dendrobium small molecule peptide and a preparation method, the hangover alleviating composition containing dendrobium small molecule peptide mainly comprises dendrobium small molecule peptide extract, the dendrobium small molecule peptide extract contains small molecule peptide DHP-1 (Gly-Ala-Val-His-Leu), DHP-2 (Pro-Tyr-Ser-Met-Gln) or DHP-3 (Asp-Ile-Thr-Lys-Cys) which are not reported, and the hangover alleviating composition further comprises auxiliary materials such as vitamin C, glucose and citric acid. After drinking, the alcohol concentration in blood is obviously lower than that of a person who does not take the alcohol-dispelling beverage within a certain period of time, so that the composition has a good alcohol-dispelling effect. After the anti-alcoholism beverage is taken, drunkenness symptoms such as dizziness, nausea and vomiting are obviously relieved, and the comfort level of a subject is improved. Liver function indexes (such as glutamic-pyruvic transaminase, glutamic oxalacetic transaminase and the like) are detected to find that the liver injury degree of a subject who drinks the anti-alcohol beverage for a long time is obviously lower than that of a single drinker, so that the composition has a protective effect on the liver.

Description

Technical Field

[0001] The invention relates to the technical field of food, and in particular to a hangover-relief composition containing dendrobium small-molecule peptides and a preparation method thereof. Background Art

[0002] Excessive drinking can cause many harms to the human body, such as liver damage, nervous system dysfunction, etc. The effects of hangover-relieving compositions on the market vary, and some compositions may have side effects. Dendrobium has many health benefits, but its application in hangover-relieving has not been fully explored, especially the mechanism of action of Dendrobium small molecule peptides in the hangover-relieving process and the development of related compositions still need to be studied. Summary of the invention

[0003] The invention provides a hangover-relief composition containing dendrobium small-molecule peptides. The main component of the composition comprises dendrobium small-molecule peptide extracts, which contain unreported small-molecule peptides DHP-1 (Gly-Ala-Val-His-Leu), DHP-2 (Pro-Tyr-Ser-Met-Gln) or DHP-3 (Asp-Ile-Thr-Lys-Cys), and auxiliary materials such as vitamin C, glucose, citric acid, etc.

[0004] Furthermore, the Dendrobium small molecule peptide extract accounts for 20-30 parts of the total weight, vitamin C accounts for 5-10 parts, glucose accounts for 30-40 parts, and citric acid accounts for 1-3 parts.

[0005] Furthermore, the preparation method comprises uniformly mixing the dendrobium small molecule peptide extract and auxiliary materials according to a formula ratio, adding an appropriate amount of water and stirring to dissolve, and preparing the hangover-relieving beverage through filtering, sterilizing and other processes.

[0006] Furthermore, the dendrobium small molecule peptide extract is extracted by enzymatic hydrolysis, including crushing the dendrobium, adding a buffer to adjust the pH value, adding a combination of papain and trypsin to perform an enzymatic hydrolysis reaction, and after the reaction, removing the residue by centrifugation, filtration, etc. to obtain the extract.

[0007] Furthermore, the small molecule peptides DHP-1, DHP-2 and DHP-3 can significantly increase the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase, and the possible mechanism is that they bind to the active sites of the enzymes to change the conformation of the enzymes.

[0008] Furthermore, the composition can reduce alcohol damage to the liver and improve the metabolic function of the liver, possibly by regulating metabolic signal pathways in liver cells to promote the repair and regeneration of liver cells.

[0009] Furthermore, after drinking alcohol, the alcohol concentration in the blood of a person who takes the composition is significantly lower than that of a person who does not take the composition within 2-3 hours.

[0010] Furthermore, a small molecule peptide composition for sobering up, wherein the small molecule peptide is DHP-1 (Gly-Ala-Val-His-Leu),

[0011] After taking the combination, DHP-2 (Pro-Tyr-Ser-Met-Gln) or DHP-3 (Asp-Ile-Thr-Lys-Cys) can significantly alleviate the symptoms of drunkenness such as dizziness, nausea and vomiting.

[0012] Beneficial technical effects of the invention:

[0013] After drinking, the alcohol concentration in the blood of the alcohol-relieving beverage of the present invention is significantly lower than that of the non-users within a certain period of time, indicating that the composition has a good alcohol-relieving effect. After taking the alcohol-relieving beverage, the symptoms of drunkenness such as dizziness, nausea, and vomiting are significantly alleviated, and the comfort of the subject is improved. By detecting liver function indicators (such as alanine aminotransferase, aspartate aminotransferase, etc.), it is found that the degree of liver damage of the subjects who drink alcohol for a long time and take the alcohol-relieving beverage is significantly lower than that of the simple drinkers, indicating that the composition has a protective effect on the liver. DETAILED DESCRIPTION

[0014] Example 1

[0015] (I) Extraction and identification of small molecule peptides from Dendrobium officinale

[0016] 1. Extraction Method

[0017] 1. Extract small molecule peptides from Dendrobium by enzymatic hydrolysis. First, crush the Dendrobium, add an appropriate amount of buffer, adjust the pH value to an appropriate range, and then add a specific enzyme (such as a combination of papain and trypsin) to perform enzymatic hydrolysis under appropriate temperature and time conditions. After the reaction is completed, remove the residue by centrifugation, filtration, etc. to obtain a small molecule peptide extract from Dendrobium.

[0018] 2. Identification

[0019] 1. Use high performance liquid chromatography-mass spectrometry (HPLC-MS) technology to identify the extracted small molecule peptides.

[0020] Three unreported small peptides were identified and named DHP-1, DHP-2 and DHP-3.

[0021] The amino acid sequences are: DHP-1 (Gly-Ala-Val-His-Leu), DHP-2 (Pro-Tyr

[0022] -Ser-Met-Gln), DHP-3 (Asp-Ile-Thr-Lys-Cys).

[0023] Comparison of single experiments with DHP-1 small molecule peptide

[0024] 1. Three groups of cell experiments were set up, namely the control group (normal liver cells, no addition of any substances), the alcohol treatment group (liver cells were added with a certain concentration of alcohol), and the DHP-1 treatment group (liver cells were first treated with alcohol and then with DHP-1 small molecule peptide).

[0025] 2. After a period of culture, the activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) were detected. The results showed that the activities of ADH and ALDH in the alcohol-treated group were significantly lower than those in the control group, while the enzyme activity in the DHP-1-treated group was significantly higher than that in the alcohol-treated group, close to the level of the control group.

[0026] 3. By using molecular fluorescence labeling technology to observe the binding of DHP-1 to the enzyme, it was found that the Gly-Ala-Val sequence of DHP-1 did bind tightly to the amino acid residues near the active site of ADH, changing the conformation of the enzyme and enhancing the catalytic activity of the enzyme.

[0027] Animal experiments

[0028] 1. Healthy mice were divided into three groups: control group (normal feeding, no alcohol and small molecule peptides), alcohol model group (given a certain amount of alcohol by oral gavage every day), and DHP-1 intervention group (given alcohol by oral gavage and DHP-1 small molecule peptides at the same time).

[0029] 2. After continuous feeding for a period of time, the livers of mice were pathologically analyzed and biochemical indicators were detected. The livers of mice in the alcohol model group showed obvious fatty degeneration and inflammatory cell infiltration, and the indicators such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly increased. The liver pathological changes of mice in the DHP-1 intervention group were significantly alleviated, and the liver function indicators were close to the control group.

[0030] Human trials

[0031] 1. Recruit volunteers and divide them into two groups: an experimental group (taking a preparation containing DHP-1 small molecule peptide after drinking) and a control group (taking a placebo after drinking).

[0032] 2. Collect blood samples at different time points after drinking to detect alcohol concentration and observe symptoms of drunkenness.

[0033] The results showed that the blood alcohol concentration of the experimental group was significantly lower than that of the control group within 2-3 hours.

[0034] The incidence and severity of drunkenness symptoms such as dizziness, nausea, and vomiting were also significantly lower than those in the control group. 1.

[0036] Cell experiments

[0037] 1. Also set up three groups of cell experiments: control group, alcohol treatment group, and DHP-2 treatment group.

[0038] 2. The activity of ADH and ALDH was detected, and it was found that the enzyme activity of the DHP-2 treatment group was significantly increased compared with the alcohol treatment group. The Pro-Tyr-

[0039] The Ser sequence has electrostatic interactions and van der Waals forces with the key amino acid residues in the active site of ALDH, thereby enhancing the activity of the enzyme.

[0040] Animal experiments

[0041] 1. Mice were divided into groups for the experiment: control group, alcohol model group, and DHP-2 intervention group.

[0042] 2. Observation of liver pathological changes and biochemical indices showed that the degree of liver fatty degeneration and inflammation in the DHP-2 intervention group was alleviated, and the liver function indices were better than those in the alcohol model group.

[0043] Human trials

[0044] 1. Volunteers were divided into an experimental group (taking a composition containing DHP-2 small molecule peptide) and a control group

[0045] (Placebo).

[0046] 2. In terms of testing blood alcohol concentration and observing symptoms of drunkenness, the experimental group performed better than the control group in all aspects.

[0047] Comparison of single experiments with DHP-3 small molecule peptide 1.

[0049] Cell experiments

[0050] 1. Set up three groups of cell experiments: control group, alcohol treatment group, and DHP-3 treatment group.

[0051] 2. Detection of enzyme activity revealed that DHP-3 can simultaneously increase the activity of ADH and ALDH. Study of its binding mode determined that the Asp-Ile-Thr sequence of DHP-3 interacts specifically with the active sites of ADH and ALDH, enhancing the catalytic ability of the enzyme.

[0052] Animal experiments

[0053] 1. Mice were divided into control group, alcohol model group and DHP-3 intervention group.

[0054] 2. Analysis of liver pathology and biochemical indicators showed that the degree of liver damage in the DHP-3 intervention group was significantly lower than that in the alcohol model group.

[0055] Human trials

[0056] 1. Volunteers were divided into an experimental group (taking a composition containing DHP-3 small molecule peptide) and a control group

[0057] (Placebo).

[0058] 2. The experimental group was significantly better than the control group in testing blood alcohol concentration and observing symptoms of drunkenness.

[0059] (II) Preparation of hangover-relieving composition

[0060] 1. Formulation

[0061] 1. The main ingredient is Dendrobium small molecule peptide extract, and it also includes auxiliary materials such as vitamin C, glucose, and citric acid.

[0062] The small molecule peptide extract of Dendrobium officinale accounts for 20-30 parts of the total weight, vitamin C accounts for 5-10 parts, glucose accounts for 30-40 parts, and citric acid accounts for 1-3 parts.

[0063] 2. Preparation method

[0064] 1. Evenly mix the dendrobium small molecule peptide extract and the auxiliary materials according to the formula ratio, then add an appropriate amount of water and stir to dissolve. After filtering, sterilization and other processes, a hangover-relief beverage is prepared.

[0065] (III) Mechanism of action of hangover-relieving composition

[0066] 1. Regulation of enzymes related to alcohol metabolism

[0067] 1. Through cell experiments, it was found that DHP-1, DHP-2 and DHP-3 can significantly increase the activity of alcohol dehydrogenase

[0068] The possible mechanism is that the small molecule peptide binds to the active site of the enzyme, changes the conformation of the enzyme, and thus enhances the catalytic activity of the enzyme.

[0069] 2. Effects on liver metabolic function

[0070] 1. Animal experiments show that the hangover-relieving composition of the present invention can reduce alcohol damage to the liver and improve the metabolic function of the liver. This may be because small molecule peptides can regulate metabolic signal pathways in liver cells and promote the repair and regeneration of liver cells.

[0071] (IV) Effect evaluation of hangover-relieving composition

[0072] 1. Reduce the concentration of alcohol in the blood

[0073] 1. The results of human clinical trials show that after drinking alcohol, the alcohol concentration in the blood of the drink of the present invention is significantly lower than that of the drinker within a certain period of time (such as 2-3 hours), indicating that the composition has a good alcohol sobering effect.

[0074] 2. Relieve symptoms of drunkenness

[0075] 1. Observation of the subjects found that after taking the hangover drink, symptoms of drunkenness such as dizziness, nausea, and vomiting were significantly alleviated, and the subjects' comfort level was improved.

[0076] 3. Protective effect on liver damage

[0077] 1. By testing liver function indicators (such as alanine aminotransferase, aspartate aminotransferase, etc.), it was found that the degree of liver damage in subjects who drank alcohol for a long time and took hangover drinks was significantly lower than that of those who drank alcohol alone, indicating that the combination has a protective effect on the liver.

Claims

1. A hangover-relieving composition containing a small molecule peptide from Dendrobium officinale, characterized in that: Its main ingredients include Dendrobium small molecule peptide extract, which contains small molecule peptides DHP-1 (Gly-Ala-Val-His-Leu), DHP-2 (Pro-Tyr-Ser-Met-Gln) or DHP-3 (Asp-Ile-Thr-Lys-Cys), and also include auxiliary materials such as vitamin C, glucose, and citric acid.

2. The hangover-relieving composition according to claim 1, characterized in that: The small molecule peptide extract of Dendrobium officinale accounts for 20-30 parts of the total weight, vitamin C accounts for 5-10 parts, glucose accounts for 30-40 parts, and citric acid accounts for 1-3 parts.

3. The hangover-relieving composition according to claim 1, characterized in that: The preparation method comprises the following steps: uniformly mixing the dendrobium small molecule peptide extract and auxiliary materials according to a formula ratio, adding a proper amount of water to stir and dissolve, and preparing the hangover-relieving beverage through filtering, sterilizing and other processes.

4. The hangover-relieving composition according to claim 1, characterized in that: The dendrobium small molecule peptide extract is extracted by enzymatic hydrolysis, which includes crushing the dendrobium, adding a buffer to adjust the pH value, adding a combination of papain and trypsin to perform an enzymatic hydrolysis reaction, and removing the residue by centrifugation, filtration, etc. after the reaction to obtain the extract.

5. The hangover-relieving composition according to claim 1, characterized in that: The small molecule peptides DHP-1, DHP-2 and DHP-3 can significantly increase the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase, and the possible mechanism is that they bind to the active sites of the enzymes to change the conformation of the enzymes.

6. The hangover-relieving composition according to claim 1, characterized in that: The composition can reduce alcohol damage to the liver and improve the metabolic function of the liver, possibly by regulating metabolic signal pathways in liver cells to promote the repair and regeneration of liver cells.

7. The hangover-relieving composition according to claim 1, characterized in that: After drinking alcohol, the alcohol concentration in the blood of a person who takes the composition is significantly lower than that of a person who does not take the composition within 2-3 hours.

8. The hangover-relieving composition according to claim 1, characterized in that: After taking the composition, the drunkenness symptoms such as dizziness, nausea, vomiting, etc. are significantly alleviated.

9. The hangover-relieving composition according to claim 1, characterized in that: The degree of liver damage in subjects who drank alcohol for a long time and took the composition was significantly lower than that in subjects who simply drank alcohol.

10. A small molecule peptide composition for sobering up, characterized in that: The small molecule peptide is DHP-1 (Gly-Ala-Val-His-Leu), DHP-2 (Pro-Tyr-Ser-Met-Gln) or DHP-3 (Asp-Ile-Thr-Lys-Cys).