Handheld optical fiber fluorescence immunochromatography instant detector and detection method

Abstract

The invention discloses a handheld optical fiber fluorescence immunochromatography instant detector and a detection method, the handheld optical fiber fluorescence immunochromatography instant detector adopts an optical fiber, a lens and a reflective mirror to form an optical path, so that an optical fiber detection module has the advantages of strong anti-interference capability, high safety, high signal-to-noise ratio and long service life. Compared with the existing fluorescence immunochromatography instant detector, the fluorescence immunochromatography instant detector reduces the use of a lens group, integrates an excitation light path, a fluorescence detection light path, a light source control circuit, a digital-to-analog conversion circuit, a display screen control circuit, a switch induction circuit and a program control output circuit, and has the advantages of small equipment size, compact structure and convenience in carrying; the device is especially suitable for narrow spaces or families.

Description

Technical Field

[0001] The invention belongs to the technical field of fluorescence detection, and in particular relates to a handheld optical fiber fluorescence immunochromatography instant detector and a detection method. Background Art

[0002] Point-of-care testing technology refers to rapid testing and analysis technology performed near or on-site, which can provide test results in a short time so that medical decisions can be made in a timely manner. Currently, point-of-care testing technology is developing rapidly, and point-of-care testing can produce test results in a short time, allowing patients to receive diagnosis and treatment more quickly.

[0003] Fluorescence immunochromatography is based on the specific binding reaction between antigen and antibody. Fluorescently labeled antibodies or antigens are fixed on the chromatographic membrane. When the substance to be tested in the sample binds to the fluorescent marker, it moves forward along the chromatographic membrane under the capillary action and specifically binds to another antibody or antigen fixed on the membrane to form an immune complex. By detecting the intensity of the fluorescent signal on the immune complex, the concentration of the substance to be tested can be determined.

[0004] In the field of fluorescent immunochromatographic instant detectors, it is crucial to quickly and accurately detect various biomarkers and pathogens. However, current fiber-optic fluorescent immunochromatographic instant detectors are large in size and not suitable for home use; they mostly use lenses to transmit light, which takes up a large part of the overall structure of the instrument and has low light transmission efficiency. Summary of the invention

[0005] The object of the present invention is to provide a handheld optical fiber fluorescence immunochromatography instant detector, which comprises an optical fiber, a lens and a reflector to form an optical path, thereby realizing the miniaturization of the fluorescence immunochromatography instant detector and improving its anti-interference ability.

[0006] The present invention also provides a corresponding detection method, which is simple to operate and can be applied to test strips of different specifications.

[0007] The technical solution of the present invention is as follows:

[0008] A handheld optical fiber fluorescence immunochromatography instant detector is used to detect a test strip, the test strip has a detection line and a reference line, the detection line and the reference line are arranged at intervals on the same side of the test strip, and the handheld optical fiber fluorescence immunochromatography instant detector comprises a housing, a main control board, a display screen and an optical fiber detection module;

[0009] The optical fiber detection module includes a first housing, a first lens, a reflector, a second lens, an optical fiber interface, an optical fiber bundle, a one-to-two optical splitter, a second housing, a first optical fiber, a third lens, a light source, a second optical fiber, an optical fiber connector, a fourth lens, an optical filter, and a photoelectric sensor;

[0010] An L-shaped channel is formed in the first shell, the L-shaped channel includes a first channel and a second channel perpendicular to each other, the first lens is arranged in the first channel, the second lens is arranged in the second channel, the reflector is arranged at the intersection of the first channel and the second channel, and the angle between the reflective surface and the first channel and the second channel is 45°;

[0011] The optical fiber bundle includes a central optical fiber in the middle and peripheral optical fibers arranged around the central optical fiber. One end of the optical fiber bundle is connected to an end of the L-shaped channel close to the second lens through an optical fiber interface, and the other end of the optical fiber bundle is connected to the central optical fiber with the second optical fiber and to the peripheral optical fiber with the first optical fiber through a one-to-two optical splitter.

[0012] The second housing has a first surface and a second surface opposite to each other, a third channel and a fourth channel are provided between the first surface and the second surface, a light source and a third lens are provided in sequence in the third channel from the first surface to the second surface, an optical fiber connector, a fourth lens, an optical filter and a photoelectric sensor are provided in sequence in the fourth channel, an end of the third channel away from the light source is connected to the second optical fiber, and the fourth channel is connected to the first optical fiber through the optical fiber connector;

[0013] The main control board is connected with the display screen, the photoelectric sensor and the light source control and supplies power to the display screen, the photoelectric sensor and the light source;

[0014] A housing cavity is formed in the housing, and the main control board and the optical fiber detection module are arranged in the housing cavity;

[0015] A sawtooth groove is provided on the side wall of one end of the housing, a moving block is slidably engaged with the sawtooth groove, a portion of the moving block is located in the accommodating cavity and connected to the first housing, the first channel is perpendicular to the extension direction of the housing and the reflector is located between the first lens and the moving block, the moving block drives the first housing to slide along the sawtooth groove so that the first lens can be aligned with the detection line and the reference line of the test paper of different lengths;

[0016] A test paper insertion port is provided on the end surface of one end of the shell with the sawtooth groove, and the test paper insertion port is parallel to the extension direction of the shell and communicated with the accommodating cavity.

[0017] In some possible implementations, the handheld optical fiber fluorescence immunochromatography instant detector further includes a first switch sensing module and a second switch sensing module, and in the direction from the test paper insertion port to the accommodating cavity, the opening of the first channel, the first switch sensing module and the second switch sensing module are sequentially arranged on the outer side wall of the first housing away from the moving block;

[0018] The first switch sensing module and the second switch sensing module are respectively connected to the main control board for control, and the main control board supplies power to the first switch sensing module and the second switch sensing module;

[0019] When the test paper enters the accommodating cavity through the test paper socket and contacts the first switch sensing module or the second switch sensing module, the first switch sensing module or the second switch sensing module transmits a signal to the main control board and the main control board controls the light source to emit laser.

[0020] In some possible implementations, the main control board includes an MCU microcontroller, an ADC circuit module, a switch sensing circuit, a display screen control circuit, and a power supply;

[0021] The ADC circuit module, the switch sensing circuit and the display screen control circuit are respectively connected to the MCU microcontroller control;

[0022] The ADC circuit module is used to receive the electrical signal sent by the photoelectric sensor, and then convert it into a digital signal and transmit it to the MCU microcontroller;

[0023] The switch sensing circuit is used to receive the sensing signals of the first switch sensing module and the second switch sensing module and transmit them to the MCU microcontroller;

[0024] The display screen control circuit receives instructions from the MCU microcontroller and controls the display content of the display screen;

[0025] The power supply supplies power to the MCU microcontroller, the ADC circuit module, the switch sensing circuit, the display screen control circuit, the display screen, the photoelectric sensor, the first switch sensing module, the second switch sensing module and the light source.

[0026] In some possible implementations, the wavelength of the light source is 365 nm.

[0027] In some possible implementations, both the central optical fiber and the peripheral optical fibers are multimode optical fibers.

[0028] In some possible implementations, the model of the MCU microcontroller is STM32 or ESP32.

[0029] In some possible implementations, the light source is an LED light source, a laser light source, or a tungsten-halogen bulb.

[0030] In some possible implementations, the filter is a 615 nm bandpass filter.

[0031] In some possible implementations, the photosensor is a PD photodiode, a PIN photodiode, an APD avalanche photodiode, a SiPM phototube, or a CMOS detector.

[0032] In some possible implementations, the optical fiber connector is FC, PC, or SMA905.

[0033] In some possible implementations, the display screen is an OLED display screen or an LCD display screen.

[0034] In some possible implementations, the first switch sensing module and the second switch sensing module are provided with sensing sheets, and sensing is performed through the sensing sheets.

[0035] In some possible implementations, the moving block moves equidistantly in the sawtooth groove.

[0036] A fluorescent immunochromatographic instant detection method, using the above-mentioned handheld optical fiber fluorescent immunochromatographic instant detection instrument for detection, comprises the following steps:

[0037] S1: insert the test paper into the accommodating cavity through the test paper;

[0038] S2: The test paper contacts the first switch sensing module, the first switch sensing module transmits a signal to the switch sensing circuit, and the MCU microcontroller controls the light source to emit 365nm excitation light;

[0039] S3: Control the moving block to move in the sawtooth groove so that the first lens is positioned just above the reference line;

[0040] S4: The excitation light passes through the third lens, the first optical fiber, the one-to-two beam splitter, the surrounding optical fiber, and the second lens in sequence, and then is reflected by the reflector and passes through the first lens, and finally irradiates the reference line, exciting the fluorescent material at the reference line position to emit incident light;

[0041] S5: The incident light passes through the first lens, the reflector, the second lens, the central optical fiber, the one-to-two optical splitter, the second optical fiber, the third lens and the filter in sequence and reaches the photoelectric sensor;

[0042] S6: The photoelectric sensor converts the optical signal into an electrical signal and transmits it to the ADC circuit module. The ADC circuit module converts the electrical signal into a digital signal and transmits it to the MCU microcontroller. The MCU microcontroller controls the display screen through the display screen control circuit. If the MCU microcontroller receives the digital signal, the display screen displays "valid, proceed to the next step", otherwise it displays "invalid".

[0043] S7: Push the test paper to the second switch sensing module, the second switch sensing module transmits the signal to the switch sensing circuit, and the MCU microcontroller controls the light source to emit 365nm excitation light;

[0044] S8: Control the moving block to move in the sawtooth groove so that the first lens is positioned just above the detection line;

[0045] S9: The excitation light passes through the third lens, the first optical fiber, the one-to-two optical splitter, the surrounding optical fiber and the second lens in sequence, is reflected by the reflector and passes through the first lens, and finally irradiates the detection line, exciting the fluorescent material at the detection line position to emit incident light;

[0046] S10: The incident light passes through the first lens, the reflector, the second lens, the central optical fiber, the one-to-two optical splitter, the second optical fiber, the third lens and the filter in sequence and reaches the photoelectric sensor;

[0047] S11: The photoelectric sensor converts the optical signal into an electrical signal and transmits it to the ADC circuit module. The ADC circuit module converts the electrical signal into a digital signal and transmits it to the MCU microcontroller. The MCU microcontroller controls the display screen through the display screen control circuit. If the MCU microcontroller receives the digital signal, the display screen displays "positive", otherwise it displays "negative".

[0048] The present invention has at least the following beneficial effects:

[0049] (1) The optical path is composed of optical fiber, lens and reflector, so that the optical fiber detection module has the advantages of strong anti-interference ability, high safety, high signal-to-noise ratio and long service life. Compared with the existing fluorescence immunochromatography instant detector, the use of lens group is reduced, and the excitation optical path, fluorescence detection optical path, light source control circuit, digital-to-analog conversion circuit, display control circuit, switch sensing circuit and programmable output circuit are integrated. It has the advantages of small size, compact structure and easy to carry, and is especially suitable for small space or home use.

[0050] (2) The movement of the first shell in the optical fiber detection module is controlled by the cooperation of the moving block and the sawtooth groove so that the optical path is aligned with the reference line or the detection line of the detection test paper, thereby achieving adaptation to most fluorescent detection test papers on the market and facilitating user use. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 This is a schematic diagram of the overall structure of a handheld optical fiber fluorescence immunochromatography instant detector according to the present invention;

[0052] Figure 2 It is a schematic diagram of the internal side view structure of a handheld optical fiber fluorescence immunochromatography instant detector according to the present invention;

[0053] Figure 3 It is a system schematic diagram of a fiber optic detection module of a handheld fiber optic fluorescence immunochromatography instant detector according to the present invention;

[0054] Figure 4 This is a circuit function block diagram of a handheld optical fiber fluorescence immunochromatography instant detector according to the present invention;

[0055] The reference numerals in the figure are: 1-main control board; 20-first shell; 21-first lens; 22-reflector; 23-second lens; 24-optical fiber interface; 25-optical fiber bundle; 26-one-to-two splitter; 27-second shell; 28-first optical fiber; 29-third lens; 210-light source; 211-second optical fiber; 212-optical fiber connector; 213-fourth lens; 214-filter; 215-photoelectric sensor; 3-display screen; 41-first switch sensing module; 42-second switch sensing module; 5-housing; 511-sawtooth groove; 512-moving block; 52-test paper socket; 53-accommodating cavity; 6-test paper; 61-reference line; 62-detection line. DETAILED DESCRIPTION

[0056] The technical solution of the present invention is further illustrated and described below through specific implementation methods. In order to facilitate the understanding of the embodiments of the present invention, the following will be further explained by taking specific embodiments as examples in conjunction with the accompanying drawings, and the embodiments do not constitute a limitation on the embodiments of the present invention.

[0057] The terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like used herein to indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific position, be constructed and operate in a specific position, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third" and the like are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0058] Unless otherwise expressly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances. The "control connection" in the following embodiments is a connection through a wire, and when each component is "control-connected" with the MCU microcontroller, it transmits a signal to the MCU microcontroller or turns on or off according to the instructions of the MCU microcontroller.

[0059] Example 1

[0060] refer to Figure 1-Figure 2The present embodiment provides a handheld fiber optic fluorescence immunochromatography instant detector for detecting a test strip 6. A reference line 61 and a detection line 62 are arranged on one side of the test strip 6 at intervals. The handheld fiber optic fluorescence immunochromatography instant detector includes a main control board 1, a fiber optic detection module, a display screen 3, a switch sensing module and a housing 5.

[0061] A receiving cavity 53 is formed in the housing 5 , and the light detection module, the switch sensing module and the main control board 1 are all received in the receiving cavity 53 , while the display screen 3 is fixed on the side wall of the housing 5 .

[0062] Specifically, the main control board 1 includes an MCU microcontroller, an ADC circuit module, a switch sensing circuit, a display screen control circuit, and a power supply. The ADC circuit module, the switch sensing circuit, and the display screen control circuit are connected to the MCU microcontroller. The ADC circuit module is used to receive the electrical signal sent by the photoelectric sensor, and then convert it into a digital signal and transmit it to the MCU microcontroller; the switch sensing circuit is used to receive the sensing signal of the first switch sensing module and the second switch sensing module and transmit it to the MCU microcontroller; the display screen control circuit receives the instruction of the MCU microcontroller and controls the display content of the display screen 3; the power supply supplies power to the MCU microcontroller, the ADC circuit module, the switch sensing circuit, the display screen control circuit, the display screen 3, the photoelectric sensor 215, the first switch sensing module 41, the second switch sensing module 42, and the light source 210.

[0063] The optical fiber detection module includes a first shell 20, a first lens 21, a reflector 22, a second lens 23, an optical fiber interface 24, an optical fiber bundle 25, a one-to-two splitter 26, a second shell 27, a first optical fiber 28, a third lens 29, a light source 210, a second optical fiber 211, an optical fiber connector 212, a fourth lens 213, a filter 214, and a photoelectric sensor 215.

[0064] refer to Figure 2 An L-shaped channel is formed in the first shell 20, and the L-shaped channel includes a first channel in the vertical direction and a second channel in the horizontal direction. A first lens 21 is installed in the first channel, and a second lens 23 and an optical fiber interface 24 are installed in the second channel from left to right. A reflector 22 is installed at the intersection of the first channel and the second channel, and its reflective surface forms an angle of 45° with the first channel and the second channel, so that the light in the first channel or the second channel is deflected 90° under the action of the reflector 22.

[0065] The optical fiber bundle 25 is a 1×N type special optical fiber including a central optical fiber in the middle and surrounding optical fibers arranged around the central optical fiber, and both the central optical fiber and the surrounding optical fibers are multimode optical fibers. One end of the optical fiber bundle 25 is connected to an end of the L-shaped channel close to the second lens 23 through an optical fiber interface 24, and the other end of the optical fiber bundle 25 is connected to the central optical fiber and the second optical fiber 211 and the surrounding optical fibers and the first optical fiber 28 through a one-to-two optical splitter 26.

[0066] The second housing 27 has a first surface and a second surface which are vertical and opposite to each other. A third channel and a fourth channel are provided between the first surface and the second surface. Figure 2 In the third channel, a light source 210 and a third lens 29 are sequentially arranged; in the fourth channel, a fiber optic connector 212, a fourth lens 213, a filter 214 and a photoelectric sensor 215 are sequentially arranged; an end of the third channel away from the light source 210 is connected to the second optical fiber 211; an end of the fourth channel away from the photoelectric sensor 215 is connected to the first optical fiber 28 via the fiber optic connector 212.

[0067] In this embodiment, the light source 210 is an LED light source, the optical fiber connector 212 is SMA905, the photoelectric sensor 215 is a CMOS detector, and the display screen 3 is an OLED display screen.

[0068] refer to Figure 2 A sawtooth groove 511 is provided on the side wall of one end of the housing 5, and a moving block 512 is slidably engaged with the sawtooth groove 511, and the moving block 512 can move equidistantly along the sawtooth groove 511. The moving block 512 is partially located in the accommodating cavity 53 and connected to the first housing 20. In the height direction, the reflector 22 is located between the first lens 21 and the moving block 512.

[0069] A test paper insertion port 52 is provided on the end surface of one end of the shell 5 having the sawtooth groove 511. The test paper insertion port 52 is parallel to the extension direction of the shell 5 and is connected to the accommodating cavity 53. The test paper 6 can enter the accommodating cavity 53 through the test paper insertion port 52. At this time, the test paper 6 is perpendicular to the first channel, so that the light passing through the first channel can be vertically irradiated on the test paper 6.

[0070] The switch sensing module includes a first switch sensing module 41 and a second switch sensing module 42, and the first switch sensing module 41 and the second switch sensing module 42 are arranged on the outer side wall of the first housing 20 away from the moving block 512. The first switch sensing module 41 and the second switch sensing module 42 are provided with sensing sheets, and the first switch sensing module 41 and the second switch sensing module 42 are controlled and connected with the switch sensing circuit of the main control board 1. When the first switch sensing module 41 or the second switch sensing module 42 contacts the test paper 6, it transmits the sensing signal to the switch sensing circuit, and then the switch sensing circuit transmits it to the MCU microcontroller, and the MCU microcontroller controls the light source to emit excitation light.

[0071] This embodiment also provides a fluorescence immunochromatography instant detection method using a handheld optical fiber fluorescence immunochromatography instant detection instrument, comprising the following steps:

[0072] S1: First, insert the test paper 6 into the accommodating cavity 53 through the test paper insertion port 52;

[0073] S2: The test paper 6 contacts the first switch sensing module 41, and the sensing sheet inside the first switch sensing module 41 transmits the signal to the switch sensing circuit, and the MCU microcontroller controls the light source 210 to emit 365nm excitation light;

[0074] S3: controlling the moving block 512 to move in the sawtooth groove 511 so that the first lens 21 is positioned directly above the reference line 61;

[0075] S4: The excitation light passes through the third lens 29, enters the first optical fiber 28, passes through the one-to-two optical splitter 26, and then enters the optical fiber bundle 25, is emitted from the other end of the optical fiber bundle 25, passes through the second lens 23, is reflected by the reflector 22, passes through the first lens 21, and finally irradiates the reference line 61 of the test paper 6, excites the fluorescent material at the position of the reference line 61, and emits incident light;

[0076] S5: The incident light emitted from the reference line 61 passes through the first lens 21, the reflector 22, and the second lens 23 in sequence, enters the central optical fiber of the optical fiber bundle 25, passes through the one-to-two optical splitter 26, enters the second optical fiber 211, is emitted from the other end, passes through the third lens 213 and the filter 214 in sequence, and reaches the photoelectric sensor 215;

[0077] S6: The photoelectric sensor 215 converts the optical signal into an electrical signal and transmits it to the ADC circuit module of the main control board 1. The ADC circuit module converts the electrical signal into a digital signal and transmits it to the MCU microcontroller. The MCU microcontroller controls the display screen control circuit. If the MCU microcontroller receives the signal, the display screen 3 displays "valid, proceed to the next step". If the MCU microcontroller does not receive the signal, the display screen 3 displays "invalid".

[0078] S7: After receiving the signal, the MCU microcontroller pushes the test paper to the second switch sensing module 42, the sensing sheet inside the second switch sensing module 42 transmits the signal to the switch sensing circuit, and the MCU microcontroller controls the light source to emit 365nm excitation light;

[0079] S8: Control the moving block 512 to move in the sawtooth groove 511 so that the first lens 21 is positioned directly above the detection line 62;

[0080] S9: The excitation light passes through the third lens 29, enters the first optical fiber 28, passes through the one-to-two optical splitter 26, and then enters the optical fiber bundle 25, is emitted from the other end of the optical fiber bundle 25, and then passes through the second lens 23, is reflected by the reflector 22, passes through the first lens 21, and finally irradiates the reference line 61 of the test paper 6, excites the fluorescent material at the position of the detection line 62, and emits incident light;

[0081] S10: The incident light emitted from the detection line 62 passes through the first lens 21, the reflector 22, and the second lens 23 in sequence, enters the central optical fiber of the optical fiber bundle 25, passes through the one-to-two optical splitter 26, enters the second optical fiber 211, is emitted from the other end, passes through the third lens 213 and the filter 214 in sequence, and reaches the photoelectric sensor 215;

[0082] S11: The photoelectric sensor 215 converts the optical signal into an electrical signal and transmits it to the ADC circuit module of the main control board 1. The ADC circuit module converts the electrical signal into a digital signal and transmits it to the MCU microcontroller. The MCU microcontroller controls the display screen control circuit. If the MCU microcontroller receives the signal, the display screen 3 displays "positive". If the MCU microcontroller does not receive the signal, the display screen 3 displays "negative".

[0083] Although the embodiments of the present application have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations on the present application. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A handheld optical fiber fluorescence immunochromatography instant detector, used for detecting a test strip, wherein the test strip has a detection line and a reference line, wherein the detection line and the reference line are arranged at intervals on the same surface of the test strip, characterized in that: The handheld optical fiber fluorescence immunochromatography instant detector comprises a housing, a main control board, a display screen and an optical fiber detection module; The optical fiber detection module includes a first housing, a first lens, a reflector, a second lens, an optical fiber interface, an optical fiber bundle, a one-to-two optical splitter, a second housing, a first optical fiber, a third lens, a light source, a second optical fiber, an optical fiber connector, a fourth lens, an optical filter, and a photoelectric sensor; An L-shaped channel is formed in the first shell, the L-shaped channel includes a first channel and a second channel perpendicular to each other, the first lens is arranged in the first channel, the second lens is arranged in the second channel, the reflector is arranged at the intersection of the first channel and the second channel, and the angle between the reflective surface and the first channel and the second channel is 45°; The optical fiber bundle includes a central optical fiber in the middle and peripheral optical fibers arranged around the central optical fiber, one end of the optical fiber bundle is connected to an end of the L-shaped channel close to the second lens through the optical fiber interface, and the other end of the optical fiber bundle is connected to the central optical fiber and the second optical fiber and to the peripheral optical fiber and the first optical fiber through a one-to-two optical splitter; The second housing has a first surface and a second surface opposite to each other, a third channel and a fourth channel are provided between the first surface and the second surface, a light source and a third lens are provided in sequence in the third channel from the first surface to the second surface, an optical fiber connector, a fourth lens, an optical filter and a photoelectric sensor are provided in sequence in the fourth channel, an end of the third channel away from the light source is connected to the second optical fiber, and the fourth channel is connected to the first optical fiber through the optical fiber connector; The main control board is connected to the display screen, the photoelectric sensor and the light source and supplies power to the display screen, the photoelectric sensor and the light source; A housing cavity is formed in the housing, and the main control board and the optical fiber detection module are arranged in the housing cavity; A sawtooth groove is provided on the side wall of one end of the shell, a moving block is slidably engaged with the sawtooth groove, a part of the moving block is located in the accommodating cavity and connected to the first shell, the first channel is perpendicular to the extension direction of the shell and the reflector is located between the first lens and the moving block, the moving block drives the first shell to slide along the sawtooth groove so that the first lens can be aligned with the detection line and the reference line of the test paper of different lengths; A test paper insertion port is formed on the end surface of one end of the shell provided with the sawtooth groove. The test paper insertion port is parallel to the extension direction of the shell and communicated with the accommodating cavity.

2. The handheld optical fiber fluorescence immunochromatography instant detector according to claim 1, characterized in that: The handheld optical fiber fluorescence immunochromatography instant detector further comprises a first switch sensing module and a second switch sensing module. From the test paper insertion port to the accommodating cavity, the opening of the first channel, the first switch sensing module and the second switch sensing module are sequentially arranged on the outer side wall of the first housing away from the moving block; The first switch sensing module and the second switch sensing module are respectively connected to the main control board for control, and the main control board supplies power to the first switch sensing module and the second switch sensing module; When the test paper enters the accommodating cavity through the test paper socket and contacts the first switch sensing module or the second switch sensing module, the first switch sensing module or the second switch sensing module transmits a signal to the main control board and the main control board controls the light source to emit laser.

3. The handheld optical fiber fluorescence immunochromatography instant detector according to claim 2, characterized in that: The main control board includes an MCU microcontroller, an ADC circuit module, a switch sensing circuit, a display screen control circuit and a power supply; The ADC circuit module, the switch sensing circuit and the display screen control circuit are respectively connected to the MCU microcontroller for control; The ADC circuit module is used to receive the electrical signal sent by the photoelectric sensor, and then convert it into a digital signal and transmit it to the MCU microcontroller; The switch sensing circuit is used to receive the sensing signals of the first switch sensing module and the second switch sensing module and transmit them to the MCU microcontroller; The display screen control circuit receives instructions from the MCU microcontroller and controls the display content of the display screen; The power supply supplies power to the MCU microcontroller, the ADC circuit module, the switch sensing circuit, the display screen control circuit, the display screen, the photoelectric sensor, the first switch sensing module, the second switch sensing module and the light source.

4. The handheld optical fiber fluorescence immunochromatography instant detector according to claim 1, characterized in that: The wavelength of the light source is 365 nm.

5. The handheld optical fiber fluorescence immunochromatography instant detector according to claim 1, characterized in that: The central optical fiber and the peripheral optical fibers are both multimode optical fibers.

6. The handheld optical fiber fluorescence immunochromatography instant detector according to claim 3, characterized in that: The model of the MCU microcontroller is STM32 or ESP32.

7. The handheld optical fiber fluorescence immunochromatography instant detector according to claim 1, characterized in that: The light source is an LED light source, a laser light source or a halogen tungsten bulb.

8. The handheld optical fiber fluorescence immunochromatography instant detector according to claim 1, characterized in that: The filter is a 615nm bandpass filter.

9. The handheld optical fiber fluorescence immunochromatography instant detector according to claim 1, characterized in that: The photoelectric sensor is a PD photodiode, a PIN photodiode, an APD avalanche photodiode, a SiPM phototube or a CMOS detector.

10. A fluorescent immunochromatography instant detection method, characterized in that: The detection is performed using the handheld optical fiber fluorescence immunochromatography instant detector according to any one of claims 1 to 9, which comprises the following steps: S1: insert the test paper into the accommodating cavity through the test paper insertion port; S2: The test strip contacts the first switch sensing module, the first switch sensing module transmits a signal to the switch sensing circuit, and the MCU microcontroller controls the light source to emit 365nm excitation light; S3: Control the moving block to move in the sawtooth groove so that the first lens is positioned just above the reference line; S4: The excitation light passes through the third lens, the first optical fiber, the one-to-two beam splitter, the surrounding optical fiber, and the second lens in sequence, and then is reflected by the reflector and passes through the first lens, and finally irradiates the reference line, thereby exciting the fluorescent material at the reference line position to emit incident light; S5: The incident light passes through the first lens, the reflector, the second lens, the central optical fiber, the one-to-two optical splitter, the second optical fiber, the third lens and the filter in sequence and reaches the photoelectric sensor; S6: The photoelectric sensor converts the optical signal into an electrical signal and transmits it to the ADC circuit module. The ADC circuit module converts the electrical signal into a digital signal and transmits it to the MCU microcontroller. The MCU microcontroller controls the display screen through the display screen control circuit. If the MCU microcontroller receives the digital signal, the display screen displays "valid, proceed to the next step", otherwise it displays "invalid". S7: Push the test strip to the second switch sensing module, the second switch sensing module transmits a signal to the switch sensing circuit, and the MCU microcontroller controls the light source to emit 365nm excitation light; S8: controlling the moving block to move in the sawtooth groove so that the first lens is positioned just above the detection line; S9: The excitation light passes through the third lens, the first optical fiber, the one-to-two optical splitter, the surrounding optical fiber and the second lens in sequence, is reflected by the reflector and passes through the first lens, and finally irradiates the detection line to excite the fluorescent material at the detection line to emit incident light; S10: The incident light reaches the photoelectric sensor after passing through the first lens, the reflector, the second lens, the central optical fiber, the one-to-two optical splitter, the second optical fiber, the third lens and the filter in sequence; S11: The photoelectric sensor converts the optical signal into an electrical signal and transmits it to the ADC circuit module. The ADC circuit module converts the electrical signal into a digital signal and transmits it to the MCU microcontroller. The MCU microcontroller controls the display screen through the display screen control circuit. If the MCU microcontroller receives the digital signal, the display screen displays "positive", otherwise it displays "negative".

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