Antibacterial material based on natural polysaccharide as well as preparation method and application of antibacterial material
By modifying dopamine, quaternized polyethyleneimine and hyaluronic acid on the surface of the polypropylene-based hernia repair sheet, the problem of hernia repair sheet being easily contaminated by bacteria is solved, and significant improvements in antibacterial performance and biocompatibility are achieved.
Patent Information
- Application Number
- CN202411869961.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2023-10-20
- Filing Date
- 2024-12-18
- Publication Date
- 2025-03-11
AI Technical Summary
Polypropylene-based hernia patches are easily contaminated by bacteria and have no antibacterial effects on their own, resulting in difficulty in infection and treatment.
Dopamine, quaternized polyethyleneimine and hyaluronic acid are modified on the surface of the polypropylene-based hernia repair sheet through chemical grafting technology to form materials with antibacterial and antifouling functions.
It significantly improves the antibacterial performance and biocompatibility of hernia repair tablets, reduces bacterial adhesion and proliferation, prolongs the antibacterial effect, and reduces the risk of infection.
Smart Images
Figure CN119591925A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of biomedical polymer materials, and specifically relates to an antibacterial material based on natural polysaccharides, a preparation method and application thereof. Background Art
[0002] Hernia patches are commonly used in hernia repair to reinforce the abdominal wall, achieve tension-free repair, and restore the biomechanics of injured tissue. At present, the use of mesh hernia patches has become the gold standard for repairing hernia defects. However, polypropylene (PP)-based hernia patches are hydrophobic and easily contaminated by bacteria, and they have no antibacterial effect. Once the infected bacteria colonize on the surface to form a dense biofilm, they will cause infection. Common treatment methods are oral or intravenous antibiotics and secondary surgery to remove materials, which will bring pain and economic burden to patients. Therefore, it is of great clinical significance to construct hernia patches with antibacterial and antifouling functions to prevent the occurrence of bacterial infection. In recent years, in order to improve the antibacterial properties of medical device surfaces, many new methods have been proposed to solve bacterial infections, such as surface loading of antibiotics, antimicrobial peptides, metal nanoparticles, photodynamic therapy, and polycationic antibacterial agents. Among them, quaternized polymers have high bactericidal efficiency by destroying the bacterial cell membrane, and also have the advantages of simple preparation, no drug resistance, and long antibacterial effect. However, quaternized polymers are positively charged and will adsorb negatively charged cells, resulting in poor biocompatibility. This is not conducive to the use of materials implanted in the body. In addition, the positively charged surface will adsorb proteins and cell fragments, becoming a place for bacteria to reproduce. Summary of the invention
[0003] Purpose of the invention: The present invention relates to an antibacterial material based on natural polysaccharides, a preparation method and application thereof, which can solve the above-mentioned problems.
[0004] Technical solution: The present invention provides an antibacterial material based on natural polysaccharides, wherein the antibacterial material comprises a base material, and the base material is modified with dopamine, quaternized polyethyleneimine and hyaluronic acid.
[0005] In some embodiments, the base material is a polypropylene mesh made of polypropylene as a polypropylene-based hernia repair patch.
[0006] In the present invention, we constructed a polypropylene-based hernia patch with antifouling and bactericidal properties by chemical grafting. Dopamine (PDA) can adhere to the surface of the material independently of the matrix, and as a platform for secondary modification, the polymer is covalently bound to the polypropylene-based hernia patch, which improves the stability and durability of the antibacterial properties. Quaternized polyethyleneimine (QPEI) with abundant reactive groups reacts with a mesh functionalized with dopamine (PDA) through a Schiff base reaction. Hyaluronic acid (HA) is an important component of the extracellular matrix (ECM) of the skin, a non-sulfated glycosaminoglycan (GAG), which plays an important role in tissue regeneration, angiogenesis and inflammatory response. Each carboxyl group in the HA structure has an anionic charge at physiological pH. HA is grafted to the surface by amidation to balance the biocompatibility and antibacterial properties of the antibacterial material. In addition, HA can also reduce the adhesion of bacteria to achieve the effect of antifouling and bactericidal.
[0007] In some embodiments, the molecular weight of the quaternized polyethyleneimine is 1800. In some specific embodiments, the antimicrobial agent is quaternized polyethyleneimine 1800 (QPEI1800).
[0008] In some embodiments, the molecular weight (Mw) of the hyaluronic acid is 200 KDa to 400 KDa.
[0009] In some embodiments, the antibacterial material is prepared by the following method:
[0010] (1-1) placing a polypropylene-based hernia repair sheet in a dopamine aqueous solution and reacting for 6 hours to 48 hours; (1-2) preparing a glutaraldehyde aqueous solution, adding the polypropylene-based hernia repair sheet obtained in step (1-1), and reacting for 1 hour to 24 hours; (S1-3) preparing an antibacterial agent quaternized polyethyleneimine aqueous solution, adding the polypropylene-based hernia repair sheet obtained in step (1-2), and reacting for 6 hours to 36 hours; (1-4) preparing a hyaluronic acid aqueous solution, adding the polypropylene-based hernia repair sheet obtained in step (1-3), and reacting for 6 hours to 36 hours to obtain an antibacterial material. The antibacterial material experimental process of the present invention is simple to operate, all reactions are carried out in an aqueous solution, and the experimental conditions are easy to implement.
[0011] In some embodiments, the concentration of glutaraldehyde in the aqueous solution is 1wt% to 50wt%. For example, the concentration of glutaraldehyde is any value of 1wt%, 2wt%, 5wt%, 10wt%, 15wt%, 20wt%, 25wt%, 30wt%, 35wt%, 40wt%, 45wt%, 50wt%, or a range consisting of any two values, such as a concentration of glutaraldehyde of 2.0wt% to 50wt%. In some embodiments, the concentration of glutaraldehyde can be adjusted as needed.
[0012] In some embodiments, the aqueous solution of dopamine refers to dopamine dissolved in a Tris-HCl aqueous solution, wherein the pH of the Tris-HCl aqueous solution is in the range of 6-9, preferably pH 8.5.
[0013] The present invention further provides a method for preparing an antibacterial material based on natural polysaccharides, comprising the following steps:
[0014] (2-1) placing a polypropylene-based hernia repair patch in a dopamine aqueous solution and reacting for 6 h to 48 h;
[0015] (2-2) preparing a glutaraldehyde aqueous solution, adding the polypropylene-based hernia repair sheet obtained in step (2-1), and reacting for 1 h to 24 h;
[0016] (2-3) preparing an aqueous solution of quaternized polyethyleneimine as an antibacterial agent, adding the polypropylene-based hernia repair sheet obtained in step (2-2), and reacting for 6 hours to 36 hours;
[0017] (2-4) Prepare a hyaluronic acid aqueous solution, add the polypropylene-based hernia repair sheet obtained in step (2-3), and react for 6 hours to 36 hours to obtain an antibacterial material.
[0018] In some embodiments, in step (2-1), the concentration of dopamine in the dopamine aqueous solution is 1 mg / mL to 3 mg / mL, such as the concentration of dopamine is any value among 1 mg / mL, 1.5 mg / mL, 2.0 mg / mL, 2.5 mg / mL, 3.0 mg / mL, or a range consisting of any two values.
[0019] In some embodiments, in step (2-1), the reaction time is any value of 1 h, 6 h, 10 h, 12 h, 15 h, 20 h, 24 h, 30 h, 35 h, 40 h, 42 h, 48 h, or a range consisting of any two values.
[0020] In some embodiments, in step (S2-1), the reaction temperature is 20°C to 30°C, such as the reaction temperature is 25°C.
[0021] In some embodiments, the polypropylene-based hernia repair sheet is subjected to the following treatment before the reaction: the polypropylene-based hernia repair sheet is cleaned by first washing it with ethanol and then with water, washing alternately for 3-5 times, and drying it.
[0022] In some embodiments, in step (2-2), the concentration of quaternized polyethyleneimine in the quaternized polyethyleneimine aqueous solution is 5wt% to 50wt%, such as the concentration of quaternized polyethyleneimine is 2wt%, 5wt%, 10wt%, 15wt%, 20wt%, 25wt%, 30wt%, 35wt%, 40wt%, 45wt%, 50wt%, any value thereof, or a range consisting of any two values.
[0023] In some embodiments, in step (2-2), the reaction time is any value of 6 h, 10 h, 12 h, 15 h, 20 h, 24 h, 30 h, 36 h, or a range consisting of any two values.
[0024] In some embodiments, in step (2-2), the reaction temperature is 20°C to 30°C, such as the reaction temperature is 25°C.
[0025] In some embodiments, in step (2-3), the concentration of hyaluronic acid in the hyaluronic acid aqueous solution is 1wt% to 50wt%, such as 1wt%, 2wt%, 5wt%, 10wt%, 15wt%, 20wt%, 25wt%, 30wt%, 35wt%, 40wt%, 45wt%, 50wt%.
[0026] In some embodiments, in step (2-3), the reaction time is any value of 6 h, 10 h, 12 h, 15 h, 20 h, 24 h, 30 h, 36 h, or a range consisting of any two values.
[0027] In some embodiments, in step (2-3), the reaction temperature is 20°C to 30°C, such as the reaction temperature is 25°C.
[0028] The present invention further provides the use of the above antibacterial material or the antibacterial material obtained by the above method for preparing the antibacterial material in preparing a hernia repair patch.
[0029] The hernia repair sheet prepared by the invention can inhibit the proliferation of bacteria such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and the like, and fungi such as Candida albicans and the like.
[0030] In some specific application examples, the antibacterial material is prepared by the following method:
[0031] (1) Clean the polypropylene-based hernia repair patch, first with ethanol and then with water, alternately washing 3 to 5 times and drying;
[0032] (2) preparing a dopamine aqueous solution, dissolving 10 mg of dopamine powder in 10 mL of Tris-HCl aqueous solution, the pH range is 6-9, preferably the pH is adjusted to 8.5, adding the polypropylene-based hernia repair sheet treated in step (1) to the dopamine aqueous solution, and reacting for 6 h to 48 h, preferably 6 h to 42 h;
[0033] (3) preparing a glutaraldehyde aqueous solution with a concentration of 1% to 50wt%, preferably 2.0wt% to 50wt%, adding the polypropylene-based hernia repair sheet obtained in step (2), and reacting for 1h to 24h, preferably 4h to 24h;
[0034] (4) preparing an antibacterial aqueous solution, wherein QPEI1800 is selected as the antibacterial agent, and the concentration of the antibacterial agent is 5wt%-50wt%, and the polypropylene-based hernia repair sheet obtained in step (3) is added thereto, and reacting for 6h-36h;
[0035] (5) Prepare a hyaluronic acid aqueous solution with a concentration of 1 wt% to 50 wt%, add the polypropylene-based hernia repair sheet obtained in step (4), and react for 6 to 36 hours.
[0036] Beneficial effects: The present invention combines QPEI and HA on the surface of PP to prepare an antibacterial material, which can be used for an anti-fouling and anti-bacterial hernia repair patch. The anti-fouling and anti-bacterial effects of the hernia repair patch prepared by the antibacterial material of the present invention are significant and lasting, and the modified hernia repair patch has good biocompatibility and low cytotoxicity. The present invention further provides a preparation method of the antibacterial material, and the preparation method of the present invention has mild and non-harsh reaction conditions. BRIEF DESCRIPTION OF THE DRAWINGS
[0037] Figure 1 The scanning electron microscope images of the polypropylene-based hernia repair patch before and after modification in Example 1 of the present invention are shown;
[0038] Figure 2 This is an antibacterial effect diagram of Example 5 of the present invention;
[0039] Figure 3 This is an antifungal effect diagram of Example 5 of the present invention;
[0040] Figure 4 This is the cytotoxicity of Example 5 of the present invention. DETAILED DESCRIPTION
[0041] Example 1: Preparation of antibacterial material
[0042] Preparation of quaternized PEI1800: Weigh 2g of polyethyleneimine PEI and dissolve it in 8mL of tetrahydrofuran, and weigh 1.833g of bromohexane and dissolve it in 8mL of tetrahydrofuran. Slowly add the PEI solution to the bromohexane solution, stir and react at 50°C for 12h, collect the supernatant after the reaction is completed, and precipitate with 10 times the amount of n-hexane under stirring. Collect the precipitate by centrifugation, the product is a yellow viscous substance, and then vacuum dry it to obtain quaternized polyethyleneimine.
[0043] Pretreatment of the polypropylene-based hernia patch: The polypropylene-based hernia patch was washed alternately with ethanol and water for 3 times and dried.
[0044] A 1 mg / mL dopamine Tris-HCL buffer solution was prepared, and the pH was adjusted to 8.5. The treated polypropylene-based hernia patch was placed in the above solution and reacted for 6 hours at a reaction temperature of 25°C. The polypropylene-based hernia patch was taken out and dried to obtain PP-PDA.
[0045] A 2.5 wt % glutaraldehyde aqueous solution was prepared, and the polypropylene-based hernia repair sheet was added thereto. The mixture was reacted at room temperature for 2 h, and the polypropylene-based hernia repair sheet was taken out and dried.
[0046] A quaternized PEI1800 aqueous solution was prepared with a concentration of 2 mg / mL, and the polypropylene-based hernia repair sheet was added thereto for reaction to obtain PP-PDA-Q.
[0047] A hyaluronic acid aqueous solution was prepared with a concentration of 1 mg / mL, and the polypropylene-based hernia repair sheet was added thereto for reaction to obtain the antibacterial material PP-PDA-Q-HA.
[0048] The scanning electron microscope images of the unmodified polypropylene hernia repair sheet PP, the dopamine-modified polypropylene hernia repair sheet PP-PDA, the dopamine- and quaternized polyethyleneimine-modified polypropylene hernia repair sheet PP-PDA-Q, and the dopamine-, quaternized polyethyleneimine- and hyaluronic acid-modified polypropylene hernia repair sheet PP-PDA-Q-HA prepared by the present invention are shown in FIG. Figure 1 shown.
[0049] Escherichia coli and Staphylococcus aureus were used for antibacterial experiments, and Candida albicans was used for antifungal experiments. The concentration of bacterial solution was 10 6 CFU, the polypropylene-based hernia patch after the reaction was co-cultured with the bacterial solution at 37°C for 4 hours. The experimental group was the modified polypropylene-based hernia patch, and the control group was the unmodified polypropylene-based hernia patch and the original polypropylene-based hernia patch (PP), respectively. The modified polypropylene-based hernia patch had an antibacterial effect of more than 80% and had good biocompatibility. It shows that the antifouling effect and antibacterial effect of the hernia patch prepared by the antibacterial material of the present invention are significant and lasting, and the modified hernia patch of the present invention has good biocompatibility and low cytotoxicity.
[0050] Example 2: Preparation of antibacterial material
[0051] Pretreatment of the polypropylene-based hernia patch: The polypropylene-based hernia patch was washed alternately with ethanol and water for 3 times and dried.
[0052] A 1.5 mg / mL dopamine Tris-HCL buffer solution was prepared, and the pH was adjusted to 8.5. The treated polypropylene-based hernia patch was placed in the above solution and reacted for 6 hours at a reaction temperature of 25° C. The polypropylene-based hernia patch was taken out and dried.
[0053] A 3.0 wt % glutaraldehyde aqueous solution was prepared, and the polypropylene-based hernia repair sheet was added thereto. The mixture was reacted at room temperature for 2 h, and the polypropylene-based hernia repair sheet was taken out and dried.
[0054] A quaternized PEI1800 (prepared by the same method as in Example 1) aqueous solution was prepared at a concentration of 2.5 mg / mL, and the polypropylene-based hernia repair sheet was added thereto for reaction.
[0055] A hyaluronic acid aqueous solution was prepared with a concentration of 2 mg / mL, and the polypropylene-based hernia repair sheet was added thereto for reaction to obtain an antibacterial material.
[0056] Escherichia coli and Staphylococcus aureus were used for antibacterial experiments, and Candida albicans was used for antifungal experiments. The concentration of bacterial solution was 10 6 CFU, the polypropylene mesh after reaction was co-cultured with bacterial solution at 37℃ for 4h. The experimental group was the modified polypropylene mesh, and the control group was the unmodified polypropylene-based hernia repair patch and the original polypropylene-based hernia repair patch. The modified polypropylene mesh had an antibacterial effect of more than 80% and good biocompatibility.
[0057] Example 3: Preparation of antibacterial material
[0058] Pretreatment of the polypropylene-based hernia patch: The polypropylene-based hernia patch was washed alternately with ethanol and water for 3 times and dried.
[0059] Prepare a 2.0 mg / mL dopamine Tris-HCL buffer solution, adjust the pH to 8.5, put the treated polypropylene mesh into the above solution, react for 6 hours at a reaction temperature of 25°C, take out the polypropylene mesh and dry it.
[0060] A 3.5 wt % glutaraldehyde aqueous solution was prepared, and the polypropylene mesh was added thereto. The mixture was reacted at room temperature for 2 h, and the polypropylene mesh was taken out and dried.
[0061] A quaternized PEI1800 (prepared by the same method as in Example 1) aqueous solution was prepared at a concentration of 3.0 mg / mL, and the polypropylene-based hernia repair sheet was added thereto for reaction.
[0062] A hyaluronic acid aqueous solution was prepared with a concentration of 3 mg / mL, and the polypropylene-based hernia repair sheet was added for reaction to obtain an antibacterial material. Escherichia coli and Staphylococcus aureus were used for antibacterial experiments, and Candida albicans was used for antifungal experiments. The bacterial solution concentration was 10 6CFU, the polypropylene-based hernia patch after the reaction was completed was co-cultured with the bacterial solution at 37°C for 4 hours. The experimental group was the modified polypropylene mesh, and the control group was the unmodified polypropylene-based hernia patch and the original polypropylene-based hernia patch. The modified polypropylene-based hernia patch had an antibacterial effect of more than 80% and had good biocompatibility.
[0063] Example 4: Preparation of antibacterial material
[0064] Pretreatment of the polypropylene-based hernia patch: The polypropylene-based hernia patch was washed alternately with ethanol and water for 3 times and dried.
[0065] Prepare a 2.5 mg / mL dopamine Tris-HCL buffer solution, adjust the pH to 8.5, put the treated polypropylene-based hernia repair sheet into the above solution, react for 6 hours at a reaction temperature of 25° C., take out the polypropylene mesh and dry it.
[0066] A 4.0 wt % glutaraldehyde aqueous solution was prepared, and the polypropylene-based hernia repair sheet was added thereto. The mixture was reacted at room temperature for 2 h, and the polypropylene-based hernia repair sheet was taken out and dried.
[0067] A quaternized PEI1800 (prepared by the same method as in Example 1) aqueous solution was prepared at a concentration of 3.5 mg / mL, and the polypropylene-based hernia repair sheet was added thereto for reaction.
[0068] A hyaluronic acid aqueous solution was prepared with a concentration of 4 mg / mL, and the polypropylene-based hernia repair sheet was added thereto for reaction to obtain an antibacterial material.
[0069] Escherichia coli and Staphylococcus aureus were used for antibacterial experiments, and Candida albicans was used for antifungal experiments. The concentration of bacterial solution was 10 6 CFU, the polypropylene-based hernia repair patch after the reaction was completed was co-cultured with the bacterial solution at 37°C for 4 hours. The experimental group was the modified polypropylene mesh, and the control group was the unmodified polypropylene mesh and the original polypropylene-based hernia repair patch. The modified polypropylene mesh had an antibacterial effect of more than 80% and had good biocompatibility.
[0070] Example 5: Preparation of antibacterial material
[0071] Pretreatment of the polypropylene-based hernia patch: The polypropylene-based hernia patch was washed alternately with ethanol and water for 3 times and dried.
[0072] A 3.0 mg / mL dopamine Tris-HCL buffer solution was prepared, and the pH was adjusted to 8.5. The treated polypropylene-based hernia repair piece was placed in the above solution and reacted for 6 hours at a reaction temperature of 25° C. The polypropylene-based hernia repair piece was taken out and dried.
[0073] A 4.5 wt % glutaraldehyde aqueous solution was prepared, and the polypropylene-based hernia repair sheet was added thereto. The mixture was reacted at room temperature for 2 h, and the polypropylene-based hernia repair sheet was taken out and dried.
[0074] A quaternized PEI1800 (prepared by the same method as in Example 1) aqueous solution was prepared at a concentration of 4.0 mg / mL, and the polypropylene-based hernia repair sheet was added thereto for reaction.
[0075] A hyaluronic acid aqueous solution was prepared with a concentration of 5 mg / mL, and the polypropylene-based hernia repair sheet was added thereto for reaction to obtain an antibacterial material.
[0076] Escherichia coli and Staphylococcus aureus were used for antibacterial experiments, and Candida albicans was used for antifungal experiments. The concentration of bacterial solution was 10 6 CFU, the polypropylene mesh after the reaction was co-cultured with the bacterial solution at 37℃ for 4h. The experimental group was the modified polypropylene-based hernia repair patch, and the control group was the unmodified polypropylene-based hernia repair patch and the original polypropylene mesh. The modified polypropylene-based hernia repair patch had an antibacterial effect of more than 80% and had good biocompatibility.
[0077] The experimental group was the modified polypropylene-based hernia patch (PP-PDA-Q-HA), and the control group was the unmodified polypropylene-based hernia patch (i.e., PP-PDA, PP-PDA-Q) and the original polypropylene-based hernia patch (PP).
[0078] The antibacterial and antifouling effects of the antibacterial material prepared in Example 5 of the present invention are shown in Figure 2 and Figure 3 The cell biocompatibility effect of the present invention can be found in Figure 4 .from Figure 2 and Figure 3 It can be seen that the polypropylene-based hernia repair patch is modified in the present invention to increase the anti-Staphylococcus aureus and Candida albicans performance of the polypropylene-based hernia repair patch material, and the antibacterial performance of the PP-PDA-Q and PP-PDA-Q-HA materials reaches more than 90%, indicating that the modification of dopamine, quaternary ammonium polyethyleneimine and hyaluronic acid can increase the antibacterial performance of the material. Figure 4 It can be seen from the results that hyaluronic acid improves the cell compatibility of PP-PDA-Q material, and PP-PAD-Q-HA material has good biocompatibility while having good antibacterial effect.
[0079] The present invention sequentially grafts dopamine, quaternized polyethyleneimine and hyaluronic acid by glutaraldehyde, that is, quaternized polyethyleneimine is modified on dopamine, and hyaluronic acid is modified on polyethyleneimine, thereby improving the problem of infection occurring when a hernia repair piece is implanted in the body. The present invention makes the hernia repair piece have the function of resisting bacterial infection by performing antibacterial and antifouling modification on the surface of the hernia repair piece. The quaternized polyethyleneimine (QPEI) selected by the present invention has good antibacterial activity, and the natural polysaccharide hyaluronic acid has excellent biocompatibility and hydrophilic antifouling property, which increases the biocompatibility of the antibacterial material while improving the antibacterial performance.
Claims
1. An antibacterial material based on natural polysaccharides, characterized in that: The antibacterial material comprises a base material, and the base material is modified with dopamine, quaternized polyethyleneimine and hyaluronic acid.
2. The antibacterial material based on natural polysaccharides according to claim 1, characterized in that: The base material includes polypropylene material.
3. The antibacterial material based on natural polysaccharides according to claim 1, characterized in that: The molecular weight of the quaternized polyethyleneimine is 1800 Da.
4. The antibacterial material based on natural polysaccharides according to claim 1, characterized in that: The molecular weight of the hyaluronic acid is 200KDa to 400KDa.
5. The antibacterial material based on natural polysaccharides according to claim 1, characterized in that: The antibacterial material is prepared by the following method: (1-1) placing a polypropylene-based hernia repair patch in a dopamine aqueous solution and reacting for 6 h to 48 h; (1-2) preparing a glutaraldehyde aqueous solution, adding the polypropylene-based hernia repair sheet obtained in step (1-1), and reacting for 1 h to 24 h; (1-3) preparing an aqueous solution of quaternized polyethyleneimine as an antibacterial agent, adding the polypropylene-based hernia repair sheet obtained in step (1-2), and reacting for 6 hours to 36 hours; (1-4) Prepare a hyaluronic acid aqueous solution, add the polypropylene-based hernia repair sheet obtained in step (1-3), and react for 6 hours to 36 hours to obtain an antibacterial material.
6. A method for preparing an antibacterial material based on natural polysaccharides, characterized in that: The following steps are involved: (2-1) placing a polypropylene-based hernia repair patch in a dopamine aqueous solution and reacting for 6 h to 48 h; (2-2) preparing a glutaraldehyde aqueous solution, adding the polypropylene-based hernia repair sheet obtained in step (2-1), and reacting for 1 h to 24 h; (2-3) preparing an aqueous solution of quaternized polyethyleneimine as an antibacterial agent, adding the polypropylene-based hernia repair sheet obtained in step (2-2), and reacting for 6 hours to 36 hours; (2-4) Prepare a hyaluronic acid aqueous solution, add the polypropylene-based hernia repair sheet obtained in step (2-3), and react for 6 hours to 36 hours to obtain an antibacterial material.
7. The method for preparing the antibacterial material according to claim 6, characterized in that: In step (2-1), the concentration of dopamine in the dopamine aqueous solution is 1 mg / mL to 3 mg / mL.
8. The method for preparing the antibacterial material according to claim 6, characterized in that: In step (2-2), the concentration of the quaternized polyethyleneimine in the aqueous solution of quaternized polyethyleneimine is 5wt% to 50wt%.
9. The method for preparing the antibacterial material according to claim 6, characterized in that: In step (2-3), the concentration of hyaluronic acid in the hyaluronic acid aqueous solution is 1wt% to 50wt%.
10. Use of the antibacterial material according to any one of claims 1 to 5 or the antibacterial material obtained by the method for preparing the antibacterial material according to any one of claims 6 to 9 in preparing a hernia repair patch.