Biological fermentation soybean milk powder organic fertilizer and preparation process thereof

Abstract

The invention provides a biological fermentation soybean milk powder organic fertilizer and a preparation process thereof, and belongs to the technical field of fertilizers. The preparation process comprises the following steps: preparing soybean milk powder; preparing a burdock root extract; preparing an echinacea purpurea extract; and preparing the organic fertilizer. According to the preparation method, bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae are activated firstly, then the activated bacillus subtilis, the activated lactobacillus plantarum and the activated saccharomyces cerevisiae are mixed according to the mass ratio of (8-10): (2-3): 1 to ferment soybeans to prepare the soybean milk powder, higher fermentation efficiency is achieved, the content of small-molecule polypeptide in the soybean milk powder can be effectively increased, and the soybean milk powder can be used for preparing the soybean milk powder. When the soybean milk powder is used as a main component of an organic fertilizer, after soybeans are fermented, organic substances such as protein in the soybeans are decomposed into small-molecule polypeptides, so that the soybean milk powder is more favorable for crops to absorb, can provide nitrogen, phosphorus, potassium and various trace elements for the crops, and can effectively promote the growth of the crops.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizers, and in particular to a bio-fermented soybean milk powder organic fertilizer and a preparation process thereof. Background Art

[0002] With the continuous development of global agriculture, fertilizers play a vital role in ensuring crop yield and quality and maintaining the stability of agricultural ecosystems. In the past few decades, traditional chemical fertilizers have become the mainstream fertilizers in agricultural production due to their ability to quickly provide crops with large amounts of major nutrients such as nitrogen, phosphorus, and potassium, greatly promoting the surge in food production.

[0003] However, long-term and single reliance on traditional fertilizers has also led to a series of increasingly serious environmental and agricultural problems. On the one hand, excessive use of fertilizers has caused serious damage to the soil structure, and soil compaction has become more and more common. Under the action of fertilizers, soil particles are tightly bonded, porosity is greatly reduced, and air permeability and water permeability become poor, making it difficult for plant roots to stretch and grow, and the absorption of water and nutrients is hindered, which ultimately leads to poor growth of crops and a decline in yield. On the other hand, the large-scale application of fertilizers has also caused soil acidification problems, changed the structure of soil microbial communities, and the number of beneficial microorganisms has dropped sharply, and harmful pathogens have grown, which not only reduces soil fertility, but also makes crops more vulnerable to diseases and pests. At the same time, excessive fertilizers flow into rivers, lakes and seas with rainwater, causing eutrophication of water bodies and crazy growth of algae, triggering a series of ecological crises, such as reduced oxygen content in water, and the death of a large number of aquatic organisms such as fish, which has a huge impact on the balance of the entire ecosystem.

[0004] Faced with the many drawbacks of traditional chemical fertilizers, organic fertilizers have gradually come into people's view as a green and sustainable fertilizer substitute. Organic fertilizers are derived from natural organic matter, such as animal and plant residues, livestock and poultry manure, etc., and their rich organic matter has unique advantages in improving soil structure. Organic matter can increase the porosity of the soil, making the soil loose and porous, significantly improving the air permeability and water retention, and creating a good growth environment for plant roots. Moreover, the nutrients in organic fertilizers are released slowly, which can meet the needs of crops at different growth stages, reduce nutrient loss, and improve nutrient utilization efficiency. In addition, organic fertilizers can promote the reproduction of soil microorganisms, build a healthy soil ecosystem, enhance the self-repair ability of the soil, reduce the occurrence of pests and diseases, and help the sustainable development of agriculture. However, the raw material composition of many organic fertilizers is relatively simple, relying solely on simple raw materials such as livestock and poultry manure or crop straw, which cannot provide comprehensive and balanced nutrition for crops and is difficult to effectively promote crop growth.

[0005] Therefore, it is necessary to propose a bio-fermented soybean milk powder organic fertilizer and a preparation process thereof that can provide comprehensive nutrition for crops to promote crop growth and development. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide a bio-fermented soybean milk powder organic fertilizer and a preparation process thereof.

[0007] A preparation process of biological fermented soybean milk powder organic fertilizer comprises the following steps:

[0008] S1: Preparation of soy milk powder

[0009] The soybeans are pretreated and mixed with elm leaves and mango peel residues, and then activated Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae are added to perform mixed fermentation, and then dried and ground to obtain soybean milk powder;

[0010] S2: Preparation of burdock root extract

[0011] The burdock root is washed and sliced, then soaked in an ascorbic acid solution, crushed, dried and extracted with an ethanol solution to obtain a burdock root extract;

[0012] S3: Preparation of Echinacea Extract

[0013] The echinacea is washed, dried, crushed and ground, added into an ethanol solution, and then subjected to ultrasonic extraction to obtain an echinacea extract;

[0014] S4: Preparation of organic fertilizer

[0015] The rapeseed meal and animal aggregate are pretreated respectively, then mixed with the burdock root extract and the echinacea extract, sprayed with activated microbial composite agent for fermentation, and then added with the soy milk powder, mixed and granulated and dried to obtain organic fertilizer.

[0016] Furthermore, S1 specifically includes the following steps:

[0017] S1.1: Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae are inoculated into LB liquid medium, MRS liquid medium and YPD liquid medium respectively, and cultured at 34-36° C., 30-34° C. and 25-30° C. for 12-24 hours respectively for activation to obtain activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae;

[0018] S1.2: Select high-quality soybeans, wash them with clean water, remove impurities and surface dirt, and then dry them. Then mix and crush them with elm leaves and mango peel residue to obtain a mixed fermentation raw material, wherein the mass ratio of soybeans, elm leaves and mango peel residue is (13-15): (3-5): 1;

[0019] S1.3: Add the activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae to the mixed fermentation raw materials, and then add purified water until the water content reaches 40-50%, then stir well, transfer to a sealed fermentation container, ferment at 30-36°C for 4-5 days, and then dry and grind to obtain soy milk powder.

[0020] Furthermore, S2 specifically includes the following steps:

[0021] S2.1: Wash and slice fresh burdock roots, then place in a 0.5% ascorbic acid solution and soak for 20-30 minutes, take out, crush and dry to obtain burdock root powder;

[0022] S2.2: Add the burdock root powder to 70% ethanol solution at a solid-liquid ratio of 1 g: (20-30) mL, heat and reflux at 50-60°C for extraction for 1-2 hours, then centrifuge and filter. Repeat the extraction 2-3 times, combine the filtrates, and then concentrate under reduced pressure and dry in vacuum to obtain a burdock root extract.

[0023] Furthermore, S3 specifically includes the following steps:

[0024] S3.1: Wash and dry fresh Echinacea, crush and grind it, then add it into 60% ethanol solution at a solid-liquid ratio of 1 g: (30-40) mL, stir and mix thoroughly to obtain Echinacea dispersion;

[0025] S3.2: The above-mentioned Echinacea dispersion is transferred to an ultrasonic extractor, and ultrasonic extraction is performed for 40-50 minutes. After filtration, the filter residue is repeatedly extracted twice, and the filtrate is combined, and then reduced pressure concentration and vacuum drying are performed to obtain the Echinacea extract.

[0026] Furthermore, S4 specifically includes the following steps:

[0027] S4.1: crushing the rapeseed meal and soaking it in clean water for 10-12 hours, then taking it out and draining it for later use to obtain pretreated rapeseed meal powder;

[0028] S4.2: washing, drying and grinding the animal bone material, and then steaming at 120-130°C and 0.2-0.4MPa for 30-40min to obtain pretreated bone meal;

[0029] S4.3: placing the pretreated rapeseed meal, pretreated bone meal, burdock root extract obtained in step S2.2, and echinacea extract obtained in step S3.2 in a mixer, stirring at 80-120 r / min for 20-30 min, then adding 20-30% by mass of clean water, and continuing stirring for 10-20 min to obtain a mixed material;

[0030] S4.4: Spray the activated microbial composite agent evenly on the surface of the mixed material while stirring, transfer it to a ventilated fermentation tank, ferment it at 25-30°C for 8-10 days, turn it over every 2-3 days, and then age it for 10-12 days to obtain a mixed fermentation product;

[0031] S4.5: adding the soybean milk powder obtained in step S1.3 to the mixed fermentation product, stirring and mixing thoroughly, and then granulating and drying to obtain organic fertilizer.

[0032] Furthermore, the inoculum size of activated Bacillus subtilis is (5-6)×10 5 CFU / g mixed fermentation raw materials, and the mass ratio of activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae is (8-10):(2-3):1.

[0033] Furthermore, the mass ratio of the pretreated rapeseed meal, the pretreated bone meal, the burdock root extract and the echinacea extract is (4-5):(2-3):(1-2):1.

[0034] Furthermore, the preparation steps of the activated microbial composite agent are as follows: Bacillus megaterium and Trichoderma harzianum are inoculated into LB liquid culture medium and PDA culture medium, respectively, and cultured at 35-37°C and 28-32°C for 12-24 hours, respectively, to obtain activated Bacillus megaterium and activated Trichoderma harzianum, and then the activated Bacillus megaterium, activated Trichoderma harzianum and the activated Bacillus subtilis obtained in step S1.1 are uniformly mixed in a mass ratio of (1-3):1:(3-4) to obtain an activated microbial composite agent.

[0035] Furthermore, the mass ratio of the activated microbial composite agent to the mixed material is 1:(20-26), and the mass ratio of the soy milk powder to the mixed fermentation product is (10-14):1.

[0036] Furthermore, a bio-fermented soy milk powder organic fertilizer is prepared by the preparation process of the bio-fermented soy milk powder organic fertilizer described in any one of the above items.

[0037] Compared with the prior art, the present invention has at least the following beneficial effects:

[0038] 1. The present invention activates Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae first, and then mixes the activated Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae to ferment soybeans to prepare soybean milk powder. After the soybeans are fermented, organic substances such as protein in the soybeans are decomposed into small molecular polypeptides, which are more conducive to crop absorption and can provide nitrogen, phosphorus, potassium and various trace elements for the crops. Therefore, when the soybean milk powder is used as the main component of organic fertilizer, it can effectively promote crop growth. In addition, when the activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae are mixed in a mass ratio of (8-10): (2-3): 1 to ferment soybeans, the fermentation efficiency is higher and the content of small molecular polypeptides in the soybean milk powder can be effectively increased.

[0039] 2. The present invention pre-treats soybeans and then mixes them with elm leaves and mango peel residues for fermentation. Since the elm leaves are rich in nutrients, they can not only provide nutrition for microbial reproduction, reduce the damage of free radicals to microorganisms during the fermentation process, and maintain the stability of the fermentation environment, but also play a role in adsorbing and fixing microorganisms, so that the microorganisms can better contact with soybeans. Mango peel residues contain a large amount of carbohydrates, organic acids and minerals, etc., which can not only provide important carbon sources, nitrogen sources and trace elements for microbial fermentation, so that the microorganisms can better grow and metabolize activities, but also the pectin therein helps the microorganisms in the fermentation substrate. Evenly distributed, it is conducive to the mutual cooperation between microorganisms, and minerals can serve as cofactors of microbial enzymes, participate in the metabolic process of microorganisms, and promote the fermentation of microorganisms. When elm leaves and mango peel residues are used in combination, the two are complementary in nutrients, which can provide more comprehensive nutrition for microorganisms, promote their balanced growth and efficient metabolism, and the nutrients and bioactive substances in elm leaves support the growth and metabolism of microorganisms, and the carbon source and regulatory substances in mango peel residues optimize the fermentation environment, so that the three microorganisms can better perform their respective functions, thereby synergistically promoting the microbial decomposition of soy protein and producing more small molecule peptides.

[0040] 3. The present invention extracts burdock root and echinacea with ethanol solution respectively to prepare burdock root extract and echinacea extract, and then mixes the two with rapeseed meal and animal bone material for fermentation and then mixes with soy milk powder to prepare organic fertilizer. Since the burdock root extract contains rich nutrients, it can participate in the physiological process of plants and improve the metabolic efficiency of plants, thereby promoting the growth and development of plants. The echinacea extract can improve the plant's absorption efficiency of nutrients in the soil by affecting the physiological function of the plant root system. When the burdock root extract and the echinacea extract are used together, the burdock root extract provides nutrients that can meet the growth and development of crops, and the echinacea extract can promote the absorption and utilization of nutrients by crops, thereby improving the nutrient supply efficiency of organic fertilizers, better meeting the nutrient requirements of crops at different growth stages, and promoting the growth and development of crops. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the present disclosure and, together with the description, further serve to explain the principles of the present disclosure and to enable those skilled in the relevant art to make and use the present disclosure.

[0042] Figure 1 This is a process flow chart for preparing the bio-fermented soybean milk powder organic fertilizer used in an embodiment of the present invention. DETAILED DESCRIPTION

[0043] The following describes in detail a bio-fermented soybean milk powder organic fertilizer and its preparation process provided by the present invention in conjunction with the accompanying drawings and specific embodiments.

[0044] Preparation Example

[0045] The steps for preparing LB liquid medium are as follows:

[0046] Add 10 g / L tryptone, 5 g / L yeast extract and 10 g / L sodium chloride into distilled water, stir to mix evenly, sterilize, and store at 4°C for later use to obtain LB liquid culture medium.

[0047] The preparation steps of MRS liquid medium are as follows:

[0048] 10 g / L peptone, 10 g / L beef extract, 5 g / L yeast extract, 20 g / L glucose, 5 g / L sodium acetate, 2 g / L dipotassium hydrogen phosphate, 0.58 g / L magnesium sulfate heptahydrate, 0.25 g / L anhydrous manganese sulfate and 1 m / L Tween 80 were added into distilled water, stirred and mixed evenly, sterilized, and stored at 4°C for later use to obtain MRS liquid culture medium.

[0049] The preparation steps of YPD liquid medium are as follows:

[0050] Add 10 g / L yeast extract, 20 g / L trypsin and 20 g / L glucose into distilled water, stir to mix evenly and sterilize, and store at 4°C for later use to obtain YPD liquid culture medium.

[0051] The preparation steps of PDA medium are as follows:

[0052] Peel and cut 200g of potatoes into pieces, add water and boil for 15min, then filter to obtain about 400mL of extract, then add 20g of glucose, stir to dissolve, add distilled water to make up the total volume to 1L, sterilize, and store at 4°C for future use to obtain PDA culture medium.

[0053] Example 1

[0054] A preparation process of biological fermented soybean milk powder organic fertilizer, such as Figure 1 As shown, the following steps are included:

[0055] S1: Preparation of soy milk powder

[0056] S1.1: Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae are inoculated into LB liquid medium, MRS liquid medium and YPD liquid medium respectively, and cultured at 34° C., 30° C. and 25° C. for 12 h respectively for activation to obtain activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae;

[0057] S1.2: Select high-quality soybeans, wash them with clean water, remove impurities and surface dirt, and then dry them. Then mix and crush them with elm leaves and mango peel residue to obtain a mixed fermentation raw material, wherein the mass ratio of soybeans, elm leaves and mango peel residue is 13:3:1;

[0058] S1.3: Add the activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae to the mixed fermentation raw materials, add purified water until the water content reaches 40%, and then stir well and transfer to a sealed fermentation container, ferment at 30°C for 4 days, and then dry and grind to obtain soy milk powder, wherein the inoculation amount of activated Bacillus subtilis is 5×10 5 CFU / g mixed fermentation raw materials, and the mass ratio of activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae is 8:2:1;

[0059] S2: Preparation of burdock root extract

[0060] S2.1: Wash and slice fresh burdock roots, then place them in a 0.5% ascorbic acid solution and soak for 20 minutes. Then, take them out, crush them and dry them to obtain burdock root powder.

[0061] S2.2: Add the burdock root powder to 70% ethanol solution at a solid-liquid ratio of 1 g:20 mL, heat and reflux at 50°C for 1 h, and then centrifuge and filter. Repeat the extraction twice, combine the filtrates, and then concentrate under reduced pressure and vacuum dry to obtain a burdock root extract.

[0062] S3: Preparation of Echinacea Extract

[0063] S3.1: Wash and dry fresh Echinacea, crush and grind it, then add it into 60% ethanol solution at a solid-liquid ratio of 1 g:30 mL, stir and mix thoroughly to obtain Echinacea dispersion;

[0064] S3.2: The Echinacea dispersion is transferred to an ultrasonic extractor, subjected to ultrasonic extraction for 40 minutes, and after filtration, the residue is subjected to repeated extraction twice, and the filtrates are combined, and then concentrated under reduced pressure and dried in vacuo to obtain an Echinacea extract;

[0065] S4: Preparation of organic fertilizer

[0066] S4.1: crushing the rapeseed meal and soaking it in clean water for 10 hours, then taking it out and draining it for later use to obtain pretreated rapeseed meal powder;

[0067] S4.2: washing, drying and grinding the animal bone material, and then steaming at 120°C and 0.2MPa for 30-40min to obtain pretreated bone meal;

[0068] S4.3: placing the pretreated rapeseed meal, pretreated bone meal, burdock root extract obtained in step S2.2 and echinacea extract obtained in step S3.2 in a mixer at a mass ratio of 4:2:1:1, stirring at 80 r / min for 20 min, then adding 20% ​​clean water by mass, and continuing stirring for 10 min to obtain a mixed material;

[0069] S4.4: Spray the activated microbial composite agent evenly on the surface of the mixed material while stirring, and then transfer it to a ventilated fermentation tank, ferment it at 25°C for 8 days, turn the pile every 2 days, and then age it for 10 days to obtain a mixed fermentation product, wherein the mass ratio of the activated microbial composite agent to the mixed material is 1:20, and the preparation steps of the activated microbial composite agent are as follows: inoculate Bacillus megaterium and Trichoderma harzianum in LB liquid culture medium and PDA culture medium respectively, and culture them at 35°C and 28°C for 12 hours respectively to activate them to obtain activated Bacillus megaterium and activated Trichoderma harzianum, and then evenly mix the activated Bacillus megaterium and activated Trichoderma harzianum with the activated Bacillus subtilis obtained in step S1.1 at a mass ratio of 1:1:3 to obtain the activated microbial composite agent;

[0070] S4.5: Add the soy milk powder obtained in step S1.3 to the mixed fermentation product, stir and mix thoroughly, and then granulate and dry to obtain organic fertilizer, wherein the mass ratio of the soy milk powder to the mixed fermentation product is 10:1.

[0071] Example 2

[0072] A preparation process of biological fermented soybean milk powder organic fertilizer, such as Figure 1 As shown, the following steps are included:

[0073] S1: Preparation of soy milk powder

[0074] S1.1: Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae are inoculated into LB liquid medium, MRS liquid medium and YPD liquid medium respectively, and cultured at 35° C., 32° C. and 28° C. for 18 h respectively for activation to obtain activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae;

[0075] S1.2: Select high-quality soybeans, wash them with clean water, remove impurities and surface dirt, and then dry them. Then mix and crush them with elm leaves and mango peel residue to obtain a mixed fermentation raw material, wherein the mass ratio of soybeans, elm leaves and mango peel residue is 14:4:1;

[0076] S1.3: Add the activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae to the mixed fermentation raw materials, add purified water until the water content reaches 45%, and then stir well and transfer to a sealed fermentation container, ferment at 33°C for 4-5 days, and then dry and grind to obtain soy milk powder, wherein the inoculum amount of activated Bacillus subtilis is 5.5×10 5 CFU / g mixed fermentation raw materials, and the mass ratio of activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae is 9:2.5:1;

[0077] S2: Preparation of burdock root extract

[0078] S2.1: Wash and slice fresh burdock roots, then place in a 0.5% ascorbic acid solution and soak for 25 minutes, take out, crush and dry to obtain burdock root powder;

[0079] S2.2: Add the burdock root powder to 70% ethanol solution at a solid-liquid ratio of 1 g: 25 mL, heat and reflux at 55°C for 1.5 h, and then centrifuge and filter. Repeat the extraction twice, combine the filtrates, and then concentrate under reduced pressure and vacuum dry to obtain a burdock root extract.

[0080] S3: Preparation of Echinacea Extract

[0081] S3.1: Wash and dry fresh Echinacea, crush and grind it, then add it into 60% ethanol solution at a solid-liquid ratio of 1 g:35 mL, stir and mix thoroughly to obtain Echinacea dispersion;

[0082] S3.2: The Echinacea dispersion was transferred to an ultrasonic extractor, and ultrasonic extraction was performed for 45 minutes. After filtration, the residue was repeatedly extracted twice, and the filtrate was combined, and then reduced pressure concentration and vacuum drying were performed to obtain the Echinacea extract;

[0083] S4: Preparation of organic fertilizer

[0084] S4.1: crushing the rapeseed meal and soaking it in clean water for 11 hours, then taking it out and draining it for later use to obtain pretreated rapeseed meal powder;

[0085] S4.2: washing, drying and grinding the animal bone material, and then steaming at 125°C and 0.3 MPa for 35 min to obtain pretreated bone meal;

[0086] S4.3: placing the pretreated rapeseed meal, pretreated bone meal, burdock root extract obtained in step S2.2 and echinacea extract obtained in step S3.2 in a mixer at a mass ratio of 4.5:2.5:1.5:1, stirring at 100 r / min for 25 min, then adding 25% clean water by mass, and continuing stirring for 15 min to obtain a mixed material;

[0087] S4.4: Spray the activated microbial composite agent evenly on the surface of the mixed material while stirring, and then transfer it to a ventilated fermentation tank, ferment it at 28°C for 9 days, turn the pile every 2 days, and then age it for 11 days to obtain a mixed fermentation product, wherein the mass ratio of the activated microbial composite agent to the mixed material is 1:23, and the preparation steps of the activated microbial composite agent are as follows: inoculate Bacillus megaterium and Trichoderma harzianum in LB liquid culture medium and PDA culture medium respectively, and culture them at 36°C and 30°C for 18h respectively to activate them to obtain activated Bacillus megaterium and activated Trichoderma harzianum, and then evenly mix the activated Bacillus megaterium and activated Trichoderma harzianum with the activated Bacillus subtilis obtained in step S1.1 at a mass ratio of 2:1:3.5 to obtain the activated microbial composite agent;

[0088] S4.5: Add the soy milk powder obtained in step S1.3 to the mixed fermentation product, stir and mix thoroughly, and then granulate and dry to obtain organic fertilizer, wherein the mass ratio of the soy milk powder to the mixed fermentation product is 12:1.

[0089] Example 3

[0090] A preparation process of biological fermented soybean milk powder organic fertilizer, such as Figure 1 As shown, the following steps are included:

[0091] S1: Preparation of soy milk powder

[0092] S1.1: Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae are inoculated into LB liquid medium, MRS liquid medium and YPD liquid medium respectively, and cultured at 36° C., 34° C. and 30° C. for 24 h respectively for activation to obtain activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae;

[0093] S1.2: Select high-quality soybeans, wash them with clean water, remove impurities and surface dirt, and then dry them. Then mix and crush them with elm leaves and mango peel residue to obtain a mixed fermentation raw material, wherein the mass ratio of soybeans, elm leaves and mango peel residue is 15:5:1;

[0094] S1.3: Add the activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae to the mixed fermentation raw materials, add purified water until the water content reaches 50%, and then stir well and transfer to a sealed fermentation container, ferment at 36°C for 5 days, and then dry and grind to obtain soy milk powder, wherein the inoculation amount of activated Bacillus subtilis is 6×10 5 CFU / g mixed fermentation raw materials, and the mass ratio of activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae is 10:3:1;

[0095] S2: Preparation of burdock root extract

[0096] S2.1: Wash and slice fresh burdock roots, then place them in a 0.5% ascorbic acid solution and soak for 30 minutes. Then, take them out, crush them and dry them to obtain burdock root powder.

[0097] S2.2: Add the burdock root powder to 70% ethanol solution at a solid-liquid ratio of 1 g:30 mL, heat and reflux at 60°C for 2 h, and then centrifuge and filter. Repeat the extraction three times, combine the filtrates, and then concentrate under reduced pressure and vacuum dry to obtain a burdock root extract.

[0098] S3: Preparation of Echinacea Extract

[0099] S3.1: Wash and dry fresh Echinacea, crush and grind it, then add it into 60% ethanol solution at a solid-liquid ratio of 1 g:40 mL, stir and mix thoroughly to obtain Echinacea dispersion;

[0100] S3.2: The Echinacea dispersion is transferred to an ultrasonic extractor, and ultrasonic extraction is performed for 50 minutes. After filtration, the residue is repeatedly extracted twice, and the filtrate is combined, and then reduced pressure concentration and vacuum drying are performed to obtain an Echinacea extract;

[0101] S4: Preparation of organic fertilizer

[0102] S4.1: crushing the rapeseed meal and soaking it in clean water for 12 hours, then taking it out and draining it for later use to obtain pretreated rapeseed meal powder;

[0103] S4.2: washing, drying and grinding the animal bone material, and then steaming at 130°C and 0.4 MPa for 40 min to obtain pretreated bone meal;

[0104] S4.3: placing the pretreated rapeseed meal, pretreated bone meal, burdock root extract obtained in step S2.2 and echinacea extract obtained in step S3.2 in a mixer at a mass ratio of 5:3:2:1, stirring at 120 r / min for 30 min, then adding 30% clean water by mass, and continuing stirring for 20 min to obtain a mixed material;

[0105] S4.4: Spray the activated microbial composite agent evenly on the surface of the mixed material while stirring, and then transfer it to a ventilated fermentation tank, ferment it at 30°C for 10 days, turn the pile every 3 days, and then age it for 12 days to obtain a mixed fermentation product, wherein the mass ratio of the activated microbial composite agent to the mixed material is 1:26, and the preparation steps of the activated microbial composite agent are as follows: inoculate Bacillus megaterium and Trichoderma harzianum in LB liquid culture medium and PDA culture medium respectively, and culture them at 37°C and 32°C for 24 hours respectively to activate them, to obtain activated Bacillus megaterium and activated Trichoderma harzianum, and then evenly mix the activated Bacillus megaterium and activated Trichoderma harzianum with the activated Bacillus subtilis obtained in step S1.1 at a mass ratio of 3:1:4 to obtain an activated microbial composite agent;

[0106] S4.5: Add the soy milk powder obtained in step S1.3 to the mixed fermentation product, stir and mix thoroughly, and then granulate and dry to obtain organic fertilizer, wherein the mass ratio of the soy milk powder to the mixed fermentation product is 14:1.

[0107] Comparative Example 1

[0108] The difference between Comparative Example 1 and Example 1 is that the mass ratio of activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae in step S1.3 is adjusted to 7:1:1.

[0109] Comparative Example 2

[0110] The difference between Comparative Example 2 and Example 1 is that the mass ratio of activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae in step S1.3 is adjusted to 11:4:1.

[0111] Comparative Example 3

[0112] The difference between Comparative Example 3 and Example 1 is that the elm leaves in step S1.2 are replaced by an equal amount of mango peel residue.

[0113] Comparative Example 4

[0114] The difference between Comparative Example 4 and Example 1 is that the mango peel residue in step S1.2 is replaced by an equal amount of elm leaves.

[0115] Comparative Example 5

[0116] The difference between Comparative Example 5 and Example 1 is that the burdock root extract in step S4.3 is replaced by an equal amount of Echinacea extract.

[0117] Comparative Example 6

[0118] The difference between Comparative Example 6 and Example 1 is that the Echinacea extract in step S4.3 is replaced by an equal amount of burdock root extract.

[0119] Comparative Example 7

[0120] The difference between Comparative Example 7 and Example 1 is that the mixed fermentation product in step S4.5 is replaced by an equal amount of soybean milk powder, that is, the prepared organic fertilizer contains only the soybean milk powder fertilizer prepared in step S1.3.

[0121] Test Case

[0122] Test 1: The proportion of small molecule polypeptides (number of amino acids ≤ 50) in the total amount of soy milk powder in the soy milk powder prepared in Examples 1-3 and Comparative Examples 1-4 was measured and calculated respectively. The results are shown in Table 1.

[0123] Table 1: Comparison of the proportion of small molecule peptides in the total amount of soy milk powder

[0124] Small molecule peptide ratio (%) Example 1 67.5 Example 2 67.2 Example 3 66.9 Comparative Example 1 60.2 Comparative Example 2 60.3 Comparative Example 3 58.6 Comparative Example 4 59.5

[0125] As can be seen from Table 1, in Comparative Examples 1 and 2, after the mass ratio of activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae was adjusted to be outside the range of (8-10): (2-3): 1, the proportion of small molecule polypeptides in the soy milk powder obtained by soybean fermentation was lower than that in Example 1. It can be seen that when soybeans are fermented by mixing activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae in a mass ratio of (8-10): (2-3): 1, the fermentation efficiency is higher and the content of small molecule polypeptides in the soy milk powder can be effectively increased.

[0126] In addition, in Comparative Examples 3 and 4, when a single mango peel residue or a single elm leaf is mixed with soybeans for fermentation, the proportion of small molecule polypeptides in the obtained soy milk powder is lower than the proportion of small molecule polypeptides in the soy milk powder obtained by simultaneously fermenting mango peel residue and elm leaves in Example 1. It can be seen that when elm leaves and mango peel residue are used in combination, the three microorganisms can better perform their respective functions, thereby synergistically promoting the microbial decomposition of soybean protein and producing more small molecule polypeptides.

[0127] Test 2:

[0128] Test method: A two-year ginseng field was selected for the test. The test field was divided into 7 groups, namely, Example 1 group, Example 2 group, Example 3 group, Comparative Example 5 group, Comparative Example 6 group, Comparative Example 7 group and blank group. The area of ​​each test area was 5m 2 , and each test area is randomly arranged. Then dig furrows between the rows in each test area, and evenly apply fertilizer according to the preset amount. The field management of the test field is consistent with that of the field. When the best harvest period of ginseng is reached, a sampling survey is carried out, and the sampling area is 1m per test area. 2 The ginseng root yield and single root weight were measured. The results are shown in Table 2.

[0129] Fertilizer dosage:

[0130] Example 1 group: The organic fertilizer prepared in Example 1 was applied at a fertilizer rate of 2 kg / 10 m 2 ;

[0131] Example 2 group: The organic fertilizer prepared in Example 2 was applied at a fertilizer rate of 2 kg / 10 m 2 ;

[0132] Example 3 group: The organic fertilizer prepared in Example 3 was applied at a fertilizer rate of 2 kg / 10 m 2 ;

[0133] Comparative Example 5: The organic fertilizer prepared in Comparative Example 5 was applied at a fertilizer rate of 2 kg / 10 m 2 ;

[0134] Comparative Example 6: The organic fertilizer prepared in Comparative Example 6 was applied at a fertilizer rate of 2 kg / 10 m 2 ;

[0135] Comparative Example 7: The organic fertilizer prepared in Comparative Example 7 was applied at a fertilizer rate of 2 kg / 10 m 2 ;

[0136] Blank group: no topdressing.

[0137] Table 2: Comparison of ginseng root yield per square meter and average weight per root

[0138] Ginseng root yield (g) Average single weight (g) Example 1 836 16.9 Example 2 845 17.1 Example 3 853 16.8 Comparative Example 5 769 14.6 Comparative Example 6 746 14.2 Comparative Example 7 708 13.4 Blank Group 623 12.7

[0139] As can be seen from Table 2, after the application of the organic fertilizers prepared by adding a single echinacea extract and a single burdock root extract in Comparative Examples 5 and 6, the yield per square meter and the average weight of a single ginseng root were lower than those in Example 1, indicating that when the burdock root extract and the echinacea extract are used in combination, the nutrient supply efficiency of the organic fertilizer can be improved, and the nutrient requirements of crops at different growth stages can be better met, thereby promoting the growth and development of crops.

[0140] In addition, after applying the organic fertilizer prepared with only soy milk powder in Comparative Example 7, the yield per square meter and the average single weight of ginseng roots were improved compared with the blank group, indicating that the soy milk powder prepared by first activating Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae and then fermenting soybeans with the activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae can promote crop growth.

[0141] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A process for preparing biological fermented soybean milk powder organic fertilizer, characterized in that: The steps include: S1: Preparation of soy milk powder The soybeans are pretreated and mixed with elm leaves and mango peel residues, and then activated Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae are added to perform mixed fermentation, and then dried and ground to obtain soybean milk powder; S2: Preparation of burdock root extract The burdock root is washed and sliced, then soaked in an ascorbic acid solution, crushed, dried and extracted with an ethanol solution to obtain a burdock root extract; S3: Preparation of Echinacea Extract The echinacea is washed, dried, crushed and ground, added into an ethanol solution, and then subjected to ultrasonic extraction to obtain an echinacea extract; S4: Preparation of organic fertilizer The rapeseed meal and animal aggregate are pretreated respectively, then mixed with the burdock root extract and the echinacea extract, sprayed with activated microbial composite agent for fermentation, and then added with the soy milk powder, mixed and granulated and dried to obtain organic fertilizer.

2. The process for preparing a bio-fermented soybean milk powder organic fertilizer according to claim 1, characterized in that: S1 includes the following steps: S1.1: Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae are inoculated into LB liquid medium, MRS liquid medium and YPD liquid medium respectively, and cultured at 34-36° C., 30-34° C. and 25-30° C. for 12-24 hours respectively for activation to obtain activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae; S1.2: Select high-quality soybeans, wash them with clean water, remove impurities and surface dirt, and then dry them. Then, mix and crush them with elm leaves and mango peel residues to obtain a mixed fermentation raw material, wherein the mass ratio of soybeans, elm leaves and mango peel residues is (13-15): (3-5): 1; S1.3: Add the above-mentioned activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae to the above-mentioned mixed fermentation raw materials, and then add purified water until the water content reaches 40-50%, then stir well, transfer to a sealed fermentation container, ferment at 30-36°C for 4-5 days, and then dry and grind to obtain soy milk powder.

3. The preparation process of a biological fermentation soybean milk powder organic fertilizer according to claim 2, characterized in that: S2 includes the following steps: S2.1: Wash and slice fresh burdock roots, then place in a 0.5% ascorbic acid solution and soak for 20-30 minutes, take out, crush and dry to obtain burdock root powder; S2.2: Add the burdock root powder to 70% ethanol solution at a solid-liquid ratio of 1 g: (20-30) mL, heat and reflux at 50-60°C for extraction for 1-2 hours, then centrifuge and filter. Repeat the extraction 2-3 times, combine the filtrates, and then concentrate under reduced pressure and dry in vacuum to obtain a burdock root extract.

4. The process for preparing a bio-fermented soybean milk powder organic fertilizer according to claim 3, characterized in that: S4 includes the following steps: S3.1: Wash and dry fresh Echinacea, crush and grind it, then add it into 60% ethanol solution at a solid-liquid ratio of 1 g: (30-40) mL, stir and mix thoroughly to obtain Echinacea dispersion; S3.2: The above-mentioned Echinacea dispersion is transferred to an ultrasonic extractor, and ultrasonic extraction is performed for 40-50 minutes. After filtration, the filter residue is repeatedly extracted twice, and the filtrate is combined, and then reduced pressure concentration and vacuum drying are performed to obtain the Echinacea extract.

5. The process for preparing a bio-fermented soybean milk powder organic fertilizer according to claim 4, characterized in that: S4 specifically includes the following steps: S4.1: crushing the rapeseed meal and soaking it in clean water for 10-12 hours, then taking it out and draining it for later use to obtain pretreated rapeseed meal powder; S4.2: Wash, dry and grind the animal aggregates, and then heat at 120-130℃, Steaming at 0.2-0.4 MPa for 30-40 min to obtain pretreated bone meal; S4.3: placing the pretreated rapeseed meal, pretreated bone meal, burdock root extract obtained in step S2.2, and echinacea extract obtained in step S3.2 in a mixer, stirring at 80-120 r / min for 20-30 min, then adding 20-30% by mass of clean water, and continuing stirring for 10-20 min to obtain a mixed material; S4.4: Spray the activated microbial composite agent evenly on the surface of the mixed material while stirring, transfer it to a ventilated fermentation tank, ferment it at 25-30°C for 8-10 days, turn it over every 2-3 days, and then age it for 10-12 days to obtain a mixed fermentation product; S4.5: adding the soybean milk powder obtained in step S1.3 to the mixed fermentation product, stirring and mixing thoroughly, and then granulating and drying to obtain organic fertilizer.

6. The process for preparing a bio-fermented soybean milk powder organic fertilizer according to claim 2, characterized in that: The inoculum size of activated Bacillus subtilis is (5-6)×10 5 CFU / g mixed fermentation raw materials, and the mass ratio of activated Bacillus subtilis, activated Lactobacillus plantarum and activated Saccharomyces cerevisiae is (8-10):(2-3):

1.

7. The process for preparing a bio-fermented soybean milk powder organic fertilizer according to claim 5, characterized in that: The mass ratio of pretreated rapeseed meal, pretreated bone meal, burdock root extract and echinacea extract is (4-5):(2-3):(1-2):

1.

8. The process for preparing a bio-fermented soybean milk powder organic fertilizer according to claim 5, characterized in that: The preparation steps of the activated microbial composite agent are as follows: Bacillus megaterium and Trichoderma harzianum are inoculated into LB liquid culture medium and PDA culture medium respectively, and cultured at 35-37°C and 28-32°C for 12-24 hours respectively to activate, thereby obtaining activated Bacillus megaterium and activated Trichoderma harzianum, and then the activated Bacillus megaterium, activated Trichoderma harzianum and the activated Bacillus subtilis obtained in step S1.1 are uniformly mixed in a mass ratio of (1-3):1:(3-4) to obtain an activated microbial composite agent.

9. The process for preparing a bio-fermented soybean milk powder organic fertilizer according to claim 5, characterized in that: The mass ratio of activated microbial composite agent to mixed material is 1: (20-26), the mass ratio of soy milk powder to mixed fermentation product is (10-14):

1.

10. A biological fermented soybean milk powder organic fertilizer, characterized in that: The organic fertilizer is prepared by the process for preparing the biological fermentation soybean milk powder organic fertilizer according to any one of claims 1 to 9.

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