Mixed double-layer spiral reaction chamber, single-layer spiral trap and atmospheric hydrogen peroxide concentration measuring device

Abstract

The mixed type double-layer spiral reaction chamber comprises a first reaction chamber and a second reaction chamber which are sequentially arranged in the sample injection direction, and the first reaction chamber comprises a first double-layer spiral pipe composed of a first spiral pipe and a second spiral pipe which are arranged in a winding mode; the second reaction chamber comprises a second double-layer spiral pipe consisting of a third spiral pipe and a fourth spiral pipe which are wound with each other; the first spiral tube is detachably connected with the third spiral tube, and the second spiral tube is detachably connected with the fourth spiral tube, so that second channels for detecting the peroxide concentration in the sample are respectively formed. Different from an existing double-channel detector, the hybrid double-layer spiral reaction chamber provided by the invention has the advantages that the common part of the two channels is increased to the maximum extent through the double spiral channels, the sensitivity of equipment is improved, the use of parts and the volume and weight of the equipment are reduced, and the portability and the real-time detection sensitivity of the equipment are improved.

Description

Technical Field

[0001] The present application belongs to the technical field of environmental monitoring, and in particular, relates to a hybrid double-layer spiral reaction chamber, a single-layer spiral trap, and a device for measuring the concentration of atmospheric hydrogen peroxide. Background Art

[0002] Atmospheric hydrogen peroxide is an important oxidant in the troposphere, with high reactivity and oxidation capacity. Its concentration level can be regarded as one of the indicators of atmospheric oxidation to some extent, and has a key influence on the formation of tropospheric O3. Hydrogen peroxide is an important factor leading to acid deposition and increased concentration of secondary aerosols, affecting ecosystems and climate change. In addition, hydrogen peroxide also has biological toxicity and human exposure risks, which can reduce the content of chlorophyll in cells and thus affect the normal growth of plants. When aerosols containing hydrogen peroxide enter the human respiratory system, they can also damage human lung epithelial cells and cause diseases such as asthma and cancer. Therefore, it is of great significance to achieve accurate measurement of atmospheric hydrogen peroxide and understand its concentration changes in the tropospheric atmosphere.

[0003] Currently, there is a lack of online analytical equipment suitable for field observation and reliable measurement of hydrogen peroxide, which makes it difficult to achieve complete online measurement of hydrogen peroxide, and leads to problems such as insufficient quantitative accuracy and insufficient stability. Therefore, it is particularly important to develop online analytical instruments for atmospheric hydrogen peroxide, which will help to achieve reliable real-time monitoring and research on the changing laws of hydrogen peroxide in the atmosphere.

[0004] The existing online analysis technology for atmospheric hydrogen peroxide is mainly based on spectroscopy, chemiluminescence and fluorescence. The detection limit of spectroscopy is high and it is not suitable for measuring atmospheric environmental concentrations. In addition, the equipment is expensive and the later maintenance cost is high, which is not conducive to promotion. The chemiluminescence method is simple but has the disadvantage of low sensitivity. Fluorescence spectrophotometry is commonly used for the determination of low-concentration hydrogen peroxide content, and has the advantages of good selectivity, high sensitivity and continuous operation. However, the equipment is large in size and high in price. For example, the AL2021 hydrogen peroxide online analyzer currently produced by Aero-Laser in Germany is this method. The current dual-channel measurement adopts two complete channels for parallel detection, and the parts used are twice that of a single channel, which leads to an increase in equipment cost, a complex instrument gas path, an increase in consumables, and difficulty in later maintenance, which limits its promotion.

[0005] Based on this, in order to save costs, simplify pipelines and facilitate flexible use, the present application provides a hybrid double-layer spiral reaction chamber, a single-layer spiral capture trap, and based on this, provides a capture-reaction-detection integrated atmospheric hydrogen peroxide concentration measurement device. Summary of the invention

[0006] In order to solve the problems existing in the prior art, the purpose of this application is to provide a hydrogen peroxide reaction device, a hydrogen peroxide capture device and a capture-reaction-detection integrated measurement device, which can minimize the use of parts and costs of the detection device, improve the commonality of the detection dual channels, reduce the discrimination effect, and increase the monitoring sensitivity of the device. Based on a single-layer spiral capture trap operating at low temperature and a hybrid double-layer spiral reaction chamber operating at high temperature, a cooling pipe and a heat pipe are used to conduct cooling / heating from the upper end to the lower end of the pipeline of the above two equipments, without the need for insulation and the state of the flow path in the pipe can be observed in real time, ensuring that the temperature of the capture trap and the reaction chamber is uniform and constant, and improving the capture efficiency and reactivity; the entire measuring device is small in design and compact in structure, and can be used for online continuous monitoring of hydrogen peroxide.

[0007] Specifically, this application involves the following aspects:

[0008] 1. A hybrid double-layer spiral reaction chamber, comprising: a first reaction chamber and a second reaction chamber arranged in sequence in a sampling direction, wherein the first reaction chamber comprises a first double-layer spiral tube composed of a first spiral tube and a second spiral tube arranged intertwined with each other, and the second reaction chamber comprises a second double-layer spiral tube composed of a third spiral tube and a fourth spiral tube arranged intertwined with each other; the first spiral tube and the third spiral tube are detachably connected to form a first channel for detecting the peroxide concentration in the test sample; and the second spiral tube and the fourth spiral tube are detachably connected to form a second channel for detecting the peroxide concentration in the test sample.

[0009] 2. A hybrid double-layer spiral reaction chamber according to item 1, wherein the second reaction chamber comprises a plurality of heaters and a plurality of heat pipes; the plurality of heaters are arranged at the lower end of the second double-layer spiral tube, and the plurality of heat pipes extend from the plurality of heaters along the central spiral axis of the second double-layer spiral tube in the middle and / or outer edge of the second double-layer spiral tube to the upper end of the second double-layer spiral tube, respectively, to keep the overall temperature of the second double-layer spiral tube constant; when the number of heat pipes extending from the middle of the second double-layer spiral tube to the upper end of the second double-layer spiral tube along the central spiral axis of the second double-layer spiral tube is not less than 2, the heat pipes extending from the middle of the second double-layer spiral tube to the upper end of the second double-layer spiral tube are evenly distributed; the heater makes the temperature of the second reaction chamber 30-45°C.

[0010] 3. A hybrid double-layer spiral reaction chamber according to item 1, wherein the material of the first spiral tube and the second spiral tube is quartz glass or polytetrafluoroethylene; the inner diameter of the first spiral tube and the second spiral tube is 1-1.2 mm; the length of the first spiral tube and the second spiral tube is 30-70 cm; the number of winding turns of the first spiral tube and the second spiral tube is 6-10 turns; and the spiral diameter after the first spiral tube and the second spiral tube are wound is 10-15 mm.

[0011] 4. A hybrid double-layer spiral reaction chamber according to item 1, wherein the material of the third spiral tube and the fourth spiral tube is quartz glass or polytetrafluoroethylene; the inner diameter of the third spiral tube and the fourth spiral tube is 1.2-1.5 mm; the length of the third spiral tube and the fourth spiral tube is 90-130 cm; the number of winding turns of the third spiral tube and the fourth spiral tube is 10-15 turns; and the spiral diameter of the third spiral tube and the fourth spiral tube after winding is 15-20 mm.

[0012] 5. A hybrid double-layer spiral reaction chamber according to item 2, wherein the number of the multiple heat pipes is 3-8; preferably, the number of the multiple heat pipes is 8; the material of the multiple heat pipes is one or two or more of pure copper, brass, combined heat pipes or nickel-chromium heating wires; and the length of the heat pipe is 120-150 cm, and the width of the heat pipe is 1-3 mm.

[0013] 6. According to the hybrid double-layer spiral reaction chamber of item 2, the material of the multiple heaters is one, two or three of copper, aluminum and stainless steel; the second reaction chamber also includes a patch temperature sensor.

[0014] 7. A single-layer spiral capture trap, comprising: a fifth spiral tube, a plurality of refrigeration components and a plurality of cooling tubes, wherein the plurality of refrigeration components are arranged at the lower end of the fifth spiral tube, and the plurality of cooling tubes respectively extend from the plurality of refrigeration components along the central spiral axis of the fifth spiral tube in the middle and / or outer edge of the fifth spiral tube to the upper end of the fifth spiral tube to keep the overall temperature of the fifth spiral tube constant; when the number of cooling tubes extending from the middle of the fifth spiral tube to the upper end of the fifth spiral tube along the central spiral axis of the fifth spiral tube is not less than 2, the cooling tubes extending from the middle of the fifth spiral tube to the upper end of the fifth spiral tube are evenly distributed; the refrigeration component makes the temperature of the fifth spiral tube 0-10°C.

[0015] 8. A single-layer spiral capture trap according to item 7, wherein the inner diameter of the fifth spiral tube is 1.8-2.2 mm; the length of the fifth spiral tube is 40-100 cm; the number of spiral turns of the fifth spiral tube is 10-16 turns; and the spiral diameter of the fifth spiral tube is 15-20 mm.

[0016] 9. A single-layer spiral capture trap according to item 7, wherein the number of the plurality of cooling tubes is 3-8; preferably, the number of the plurality of heat conducting tubes is 8; the material of the plurality of cooling tubes is pure copper and / or brass; and the length of the cooling tubes is 120-150 cm, and the width of the cooling tubes is 1-3 mm.

[0017] 10. According to the single-layer spiral capture trap of item 7, the material of the multiple refrigeration components is one, two or three of brass, pure copper and aluminum; the single-layer spiral capture trap also includes a patch-type temperature sensor.

[0018] 11. A device for measuring atmospheric hydrogen peroxide concentration, comprising: a capture module, a reaction module, a detection module and a calculation module; the capture module is connected to the input end pipeline of the reaction module, and is used to inhale a detection sample from the atmosphere, and capture the peroxide in the detection sample to obtain a capture liquid sample containing the detection sample; the capture module divides the capture liquid sample into sample A and sample B and transports them to the reaction module respectively, processes them respectively in the reaction module and obtains two fluorescent reaction liquid samples through fluorescent reaction after processing; the detection module is connected to the output end pipeline of the reaction module, and is used to receive two fluorescent reaction liquid samples and respectively detect the total peroxide concentration in the two fluorescent reaction liquid samples; the calculation module calculates the hydrogen peroxide concentration in the detection sample based on the total peroxide concentration in the two fluorescent reaction liquid samples.

[0019] 12. A device for measuring the atmospheric hydrogen peroxide concentration according to item 11, wherein the capture module includes an absorption liquid pump, a liquid flow controller, a gas three-way valve, a single-layer spiral capture trap as in any one of items 7-10, and a gas-liquid separation unit, wherein the upper end of the single-layer spiral capture trap is respectively connected to the gas three-way valve and the absorption liquid pump pipeline; the gas three-way valve passes the test sample and / or the zero gas sample into the single-layer spiral capture trap; the absorption liquid pump passes the absorption liquid into the single-layer spiral capture trap and forms an absorption liquid film on the inner wall of the fifth spiral tube to capture the peroxide in the test sample and obtain the capture liquid sample; the lower end of the single-layer spiral capture trap is connected to the gas-liquid separation unit pipeline, the gas-liquid separation unit performs gas-liquid separation on the capture liquid sample and the remaining gas, and divides the capture liquid sample into sample A and sample B to be transported to the reaction module respectively.

[0020] 13. A device for measuring atmospheric hydrogen peroxide concentration according to item 11, wherein the reaction module includes a hybrid double-layer spiral reaction chamber of any one of items 1-6; the first spiral tube obtains sample A and parallel liquid respectively through a peristaltic pump and mixes sample A and parallel liquid to obtain a mixed solution A, the second spiral tube obtains sample B and reaction liquid respectively through a peristaltic pump and mixes sample B and reaction liquid to obtain a mixed solution B; the third spiral tube obtains mixed solution A and derivative liquid respectively from the lower end of the first spiral tube through a peristaltic pump and mixes mixed solution A and derivative liquid to obtain fluorescent reaction liquid sample A; the fourth spiral tube obtains mixed solution B and derivative liquid respectively from the lower end of the second spiral tube through a peristaltic pump and mixes mixed solution B and derivative liquid to obtain fluorescent reaction liquid sample B.

[0021] 14. A device for measuring the atmospheric hydrogen peroxide concentration according to item 11, wherein the detection module includes a fluorescence detection chamber for detecting a fluorescent reaction liquid sample A and a fluorescence detection chamber for detecting a fluorescent reaction liquid sample B; the fluorescence detection chamber for detecting a fluorescent reaction liquid sample A and the fluorescence detection chamber for detecting a fluorescent reaction liquid sample B are respectively connected to the lower end of the third spiral tube and the lower end of the fourth spiral tube.

[0022] 15. A method for measuring hydrogen peroxide concentration, comprising the following steps:

[0023] Sampling process: capturing peroxides in the sample to be detected input from the outside, passing the sample to be detected through an absorption liquid so that the peroxides therein are absorbed by the absorption liquid, and obtaining a captured liquid containing the sample to be detected; reaction process: dividing the processed sample to be detected obtained after gas-liquid separation of the captured liquid into sample A and sample B on average, and reacting them respectively to obtain a dyeing reaction liquid; detection process: based on the dyeing reaction liquid, detecting the total peroxide concentration P in sample A and the concentration Q of peroxide after removing hydrogen peroxide from sample B; calculation process: calculating the concentration M of hydrogen peroxide in the sample to be detected, wherein M=(PQ); preferably, the measurement method further comprises the following steps: performing a calibration process before measurement, during calibration, using zero gas as the sample gas to be detected to undergo a sampling process, a reaction process, and a detection process, to determine whether the pipeline corresponding to sample A and the pipeline corresponding to sample B remain consistent, and the zero gas is high-purity nitrogen or compressed air.

[0024] Beneficial Effects

[0025] The hybrid double-layer spiral reaction chamber, single-layer spiral trap and atmospheric hydrogen peroxide concentration measurement device provided by the present application have the following technical effects:

[0026] 1. The measuring device for atmospheric hydrogen peroxide concentration provided by the present application utilizes a dual-channel fluorescence method to measure hydrogen peroxide. This method has the advantages of good selectivity and high sensitivity, but the conventional measuring equipment has complex pipelines, and the equipment on the market can only apply the capture, reaction and measurement methods of the overall dual-channel design mode; in addition, the parts used in the existing equipment are complicated, resulting in high overall equipment costs, which limits the promotion of detection technology. Therefore, the measuring device provided by the present application is optimized in structural design, and the channels after entering the hybrid double-layer spiral reaction chamber are separated by pipelines, and the capture pipelines before the reaction chamber are integrated into one, which not only simplifies the pipelines, but also effectively avoids the use of a large number of equipment parts, thereby reducing the volume and manufacturing costs; in addition, the measuring device provided by the present application also increases the commonality between the dual channels used for the reaction between the object to be detected and the detection reagent, increases its measurement parallelism, and avoids the discrimination effect that affects the measurement accuracy.

[0027] 2. The atmospheric hydrogen peroxide concentration measurement device provided in the present application is specifically designed with an integrated single-layer spiral trap. In order to ensure its capture efficiency, a contact refrigeration structure is provided, in which a fast temperature-conducting cooling tube is arranged in parallel to the upstream, middle and downstream of the sample flow direction of the single-layer spiral trap, and a refrigeration component is placed at one end of the cooling tube. The efficient cooling property of the cooling tube is used to quickly transfer the temperature to the single-layer spiral trap, thereby ensuring the uniformity of the temperature inside the trap and avoiding the reduction of the capture efficiency due to temperature differences. In addition, the single-layer spiral trap is made of quartz glass, and the gas-liquid state therein can be observed in real time through the trap without insulation measures, thereby effectively preventing malfunctions.

[0028] 3. The atmospheric hydrogen peroxide concentration measurement device provided in the present application specifically designs a hybrid double-layer spiral reaction chamber with a double-helix channel configuration, and integrates the dual-channel reaction chambers in parallel, which not only ensures the parallelism between the dual channels, but also effectively reduces the volume of the device required for the hydrogen peroxide enzymatic hydrolysis reaction and the fluorescence reaction parts; in addition, the measurement device provided in the present application is also designed with a heating structure for uniformly heating the hybrid double-layer spiral reaction chamber, and the heaters are uniformly arranged in parallel at multiple positions of the reaction channel along the upstream, middle and downstream of the flow direction of the detection sample to effectively provide the high temperature conditions required for the dual-channel reaction, ensure that the internal temperature of the hybrid double-layer spiral reaction chamber is uniformly distributed, avoid the reduction in reaction efficiency caused by uneven temperature and the discrimination effect between the dual channels, so as to produce high-quality fluorescent substances and be used for hydrogen peroxide concentration detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The figure shows a schematic structural diagram of a hybrid double-layer spiral reaction chamber according to an embodiment of the present application.

[0030] Figure 2 The figure shows a schematic structural diagram of a double-layer spiral trap according to an embodiment of the present application.

[0031] Figure 3 The figure shows a schematic structural diagram of a device for measuring atmospheric hydrogen peroxide concentration according to an embodiment of the present application.

[0032] Figure 4 The figure illustrates a flow chart of a method for measuring hydrogen peroxide concentration according to an embodiment of the present application.

[0033] Figure 5 The figure illustrates a flow chart of performing zero gas detection according to the method for measuring hydrogen peroxide concentration in an embodiment of the present application. DETAILED DESCRIPTION

[0034] The present application is further described below in conjunction with examples. It should be understood that the examples are only used to further describe and illustrate the present application and are not used to limit the present application.

[0035] Unless otherwise defined, the technical and scientific terms in this specification have the same meaning as those generally understood by those skilled in the art. Although similar or identical methods and materials as described herein may be used in experiments or practical applications, materials and methods are described herein below. In the event of a conflict, the present specification including the definitions therein shall prevail. In addition, materials, methods and examples are provided for illustration only and are not restrictive. The present application is further described below in conjunction with specific embodiments, but is not intended to limit the scope of the present application.

[0036] definition

[0037] Trap

[0038] A trap is a device used to capture and separate target compounds. When the gas containing the target compound passes through the trap, the target compound is absorbed by the ions or molecules in the solution pre-configured in the trap, and is thus enriched and retained in the solution. As the reaction proceeds, the target compound may precipitate out in the form of a precipitate, or be discharged with the solution, thereby improving the purity of the gas or obtaining an absorption solution for the target compound.

[0039] Derivative fluid

[0040] Derivatization is a reagent that can react chemically with specific components in a sample, thereby improving the sensitivity and accuracy of target detection by generating new, easily detectable compounds, i.e., derivative products. Direct detection may be very difficult for certain compounds with high polarity, high boiling point, poor thermal stability, or weak UV absorption. Through derivatization, they can be converted into compounds with low polarity, good volatility, strong thermal stability, or significant UV absorption, thereby improving their detectability in chromatographic or spectral analysis. Derivatization can significantly increase the signal intensity in mass spectrometry, thereby improving detection sensitivity.

[0041] Zero gas sample

[0042] A gas sample used to adjust or set the minimum scale (i.e., zero point) of a gas analyzer. When this gas sample enters a specific analytical instrument, the analytical instrument should display a reading of zero or close to zero. In order to ensure the accuracy of the baseline measurement, the zero gas sample should not contain any components to be measured or any impurities that may interfere with the measurement, but it may contain components that are not related to the measurement.

[0043] At present, the measurement of atmospheric hydrogen peroxide on the market mainly adopts the dual-channel fluorescence measurement method of peroxide. Specifically, peroxide reacts with a fluorescent agent under the action of peroxidase to produce a fluorescent dimer. Therefore, a dual-channel measurement method is adopted, that is, one channel determines the amount of total peroxide according to the fluorescence signal generated by the total peroxide, and one channel adds catalase that can quickly and completely destroy hydrogen peroxide in the peroxide in advance. According to the fluorescence signal generated by the total peroxide after the reaction and the organic peroxide content after the removal of hydrogen peroxide, the two test results are subtracted to obtain the hydrogen peroxide content. This method is simple, highly selective and highly sensitive, but due to the large number of pipelines and components required for the dual channel, the overall cost of the detection equipment is expensive and bulky, which limits its promotion and application.

[0044] Based on the current technical problems in the field of real-time detection of hydrogen peroxide, the present application provides a hybrid double-layer spiral reaction chamber for fluorescence characterization of hydrogen peroxide based on dual-channel fluorescence photometry, such as Figure 1 As shown, and a single-layer spiral trap, such as Figure 2 As shown, it is used to perform the fluorescence reaction treatment and liquid phase capture of atmospheric hydrogen peroxide respectively. Figure 1 As shown, it includes a first reaction chamber and a second reaction chamber arranged in sequence in the injection direction, wherein the first reaction chamber is used to perform hydrogen peroxide enzymatic hydrolysis on a liquid sample having hydrogen peroxide dissolved therein. Therefore, the first reaction chamber is designed to include a first double-layer spiral tube consisting of a first spiral tube and a second spiral tube arranged to be wound around each other, and two liquid samples having the same hydrogen peroxide content and containing the same mass of substances that can react with catalase, or containing no substances that can react with catalase, can be processed in the first reaction chamber and the second reaction chamber, respectively, to obtain samples having different hydrogen peroxide contents.

[0045] In some possible embodiments, in order to ensure that the liquid sample can decompose the hydrogen peroxide therein with high efficiency after being mixed with catalase in the first reaction chamber, the size design of the first double-layer spiral tube is very strict. Since the first reaction chamber and the second reaction chamber are successively arranged in the hybrid double-layer spiral reaction chamber, and in order to ensure that the volume of the hybrid double-layer spiral reaction chamber is as small as possible, the length of the first double-layer spiral tube needs to be further shortened relative to the enzymatic hydrolysis channel of the existing dual-channel instrument, which may cause the problem of insufficient enzymatic hydrolysis. In the first spiral tube or the second spiral tube, the liquid sample and the catalase solution cannot be fully contacted or the contact time cannot be guaranteed. This can be achieved by increasing the number of windings of the first double-layer spiral tube so that the liquid sample passes a sufficiently long distance in the first reaction chamber; when the number of windings is constant, the spiral diameter of the first double-layer spiral tube can be further increased to extend the enzymatic hydrolysis time.

[0046] However, although this method of improving the decomposition of hydrogen peroxide can make up for the inadequate reaction caused by reducing the volume of the reaction chamber, the second reaction chamber usually needs to be equipped with a heating component to provide reaction conditions for the fluorescent reaction of peroxide. If the spiral diameter of the first double-layer spiral tube is larger than the diameter of the second double-layer spiral tube in the second reaction chamber, the first double-layer spiral tube may be heated unevenly. The optimum reaction temperature of catalase is 30-40°C. An excessively large spiral diameter may cause uneven temperatures between the inner and outer edges of the first double-layer spiral tube, and the efficiency of hydrogen peroxide enzymatic hydrolysis of liquid samples will be affected.

[0047] In addition, the axial propulsion force of the liquid in the spiral tube is inversely proportional to the tube diameter. The increase in the number of windings will affect the flow state and dynamic parameters of the absorption liquid. For example, increasing the pitch will weaken the turbulence intensity in the tube to a certain extent, and increasing the tube diameter will significantly increase the intensity of the turbulence of the fluid in the tube. These changes will ultimately affect the heat transfer performance of the flow absorption liquid. In this way, it is necessary to maintain a certain ratio between the length, number of windings and diameter of the first double-layer spiral tube and the second double-layer spiral tube, so that the reaction efficiency of the liquid sample in the spiral reaction chamber is maintained at the optimal level, so as to complete the enzymatic hydrolysis and fluorescence reaction that usually requires a longer straight tube in a spiral tube with limited axial length.

[0048] Another issue that needs attention is that in order to fully mix the liquid sample and catalase in the first reaction chamber, it is also necessary to ensure that the inner diameters of the first spiral tube and the second spiral tube can provide sufficient space for the mixing of the two solutions. Therefore, the inner diameters of the first spiral tube and the third spiral tube and their winding turns and spiral diameters also need to maintain an optimal ratio, so that the first double-layer spiral tube can ensure the efficient enzymatic hydrolysis of hydrogen peroxide. In this way, the first double-layer spiral tube forms a circulation of the liquid sample and the reactant therein through the action of rotation and centrifugal force, ensuring that the mixed solution is evenly distributed in the first spiral tube or the second spiral tube, avoiding the dead corner and stratification of the mixed solution in the container due to turbulence, thereby accelerating the enzymatic hydrolysis speed and shortening the enzymatic hydrolysis time, and achieving the purpose of reducing the volume.

[0049] In some possible embodiments, the inner diameter of the first spiral tube and the second spiral tube is set to 1-1.2mm, and the length of the first spiral tube and the second spiral tube is set to 30-90cm to ensure that the volume is reduced as much as possible while ensuring that the enzymatic hydrolysis is fully carried out; the number of windings of the first spiral tube and the second spiral tube is set to 6-10 turns, and the spiral diameter of the first spiral tube and the second spiral tube after winding is 10-15mm, so that the temperature distribution of the first double-layer spiral tube as a whole is uniform, and the high / low temperature at some positions is prevented from affecting the enzyme activity. In particular, in an advantageous embodiment, the length of the first spiral tube and / or the second spiral tube is preferably 90cm, and on this basis, it is further preferred that the number of windings of the first spiral tube and / or the second spiral tube is 9 turns and the inner diameter is 1.1mm.

[0050] It can be understood that the material of the first spiral tube and the second spiral tube can be advantageously selected from quartz glass or polytetrafluoroethylene, in particular, quartz glass, so that the experimenter or operator can observe the reaction and gas-liquid conditions of the liquid sample through the first spiral tube and the second spiral tube in real time when using the hybrid double-layer spiral reaction chamber according to the embodiment of the present application, and avoid the entry of bubbles and the subsequent mutation of the measurement signal. In addition, the functions of the first spiral tube and the second spiral tube can be replaced by each other, for example, a catalase solution is added to the first spiral tube for hydrogen peroxide enzymolysis, and deionized water is added to the second spiral tube so as not to consume hydrogen peroxide, or a catalase solution is added to the second spiral tube for hydrogen peroxide enzymolysis, and deionized water is added to the first spiral tube so as not to consume hydrogen peroxide, and the use of the two spiral tubes can be adjusted according to actual conditions.

[0051] After obtaining a liquid sample and a reaction solution, such as a mixed solution of a catalase solution and a liquid sample and a parallel solution, such as a mixed solution of ultrapure water, respectively, by using the first reaction chamber, it is only necessary to add a derivative solution to the mixed solution to establish a peroxide concentration standard curve of the liquid sample by detecting the fluorescence spectrum of the fluorescent dye in the derivative solution and calculate the peroxide concentration. The fluorescence reaction process can be carried out immediately in the second reaction chamber, and the second reaction chamber includes a second double-layer helical tube composed of a third helical tube and a fourth helical tube arranged intertwined with each other, as a double-helical channel for the reaction. It is understandable that during the fluorescence reaction process, it is necessary to provide a balanced and stable reaction temperature for the two reaction channels of the second reaction chamber, which requires the second double-layer helical tube to be heated evenly.

[0052] It is understandable that, when the heating structure is determined along the injection direction, the heating distance of the spiral structure perpendicular to the injection direction is distributed in a periodic manner, such as a sinusoidal distribution, so the second double-layer spiral tube is more likely to have uneven temperature than the existing fully dual-channel reaction path. Therefore, the spiral diameter of the second double-layer spiral tube should not be too large, and the number of windings should not be too dense, but a certain duty cycle should be maintained between each winding, which is different from the second double-layer spiral tube. Therefore, in some possible embodiments, the length of the third spiral tube and the fourth spiral tube is set between 90-130cm to ensure the full progress of the fluorescence reaction, while minimizing the overall length of the second double-layer spiral tube; after determining the length, the number of windings of the third spiral tube and the fourth spiral tube can be set between 10-15 turns to ensure the full progress of the fluorescence reaction of the liquid sample and the derivative liquid in the tube.

[0053] Further, in order to maintain a certain distance between each turn of the second double-layer spiral tube so that each turn is heated evenly perpendicular to the injection direction, after determining the length and spiral diameter, the spiral diameter of the third spiral tube and the fourth spiral tube after winding can be set between 15-20 mm in accordance with the size of the spiral diameter, which can ensure that the radial area of ​​each turn will not be too large to expand the volume of the entire hybrid double-layer spiral reaction chamber, and the heat emitted by the heating component can be better conducted through the air at the non-contact part of each turn. Finally, the inner diameter of the third spiral tube and the fourth spiral tube can be set between 1.2-1.5 mm to ensure that the liquid sample and the derivative liquid fully form a circulation in the tube, and the reaction process is more efficient than that of a fully dual-channel instrument. In particular, in an advantageous embodiment, the length of the third spiral tube and / or the fourth spiral tube is preferably 120 cm, and on this basis, it is further preferred that the number of windings of the third spiral tube and / or the fourth spiral tube is 13 and the inner diameter is 1.5 mm.

[0054] It can be understood that the material of the third spiral tube and the fourth spiral tube can be advantageously selected from quartz glass or polytetrafluoroethylene, and in particular, quartz glass can also be selected, so that when the experimenter or operator uses the hybrid double-layer spiral reaction chamber according to the embodiment of the present application, the reaction of the liquid sample and the derivative liquid and the gas-liquid situation can be observed through the third spiral tube and the fourth spiral tube in real time, avoiding bubbles interfering with the reaction and causing a sudden change in the measurement signal. In addition, the functions of the third spiral tube and the fourth spiral tube can be replaced with each other, for example, adding a sample decomposed by hydrogen peroxide to the third spiral tube for a fluorescence reaction, and adding a sample not decomposed by hydrogen peroxide to the fourth spiral tube for a fluorescence reaction, or adding a sample decomposed by hydrogen peroxide to the fourth spiral tube for a fluorescence reaction, and adding a sample not decomposed by hydrogen peroxide to the third spiral tube for a fluorescence reaction, and the use of the two spiral tubes can be adjusted according to actual conditions.

[0055] In some possible embodiments, the first spiral tube and the third spiral tube together form a first channel for detecting the concentration of peroxide in the liquid sample, and the second spiral tube and the fourth spiral tube together form a second channel for detecting the concentration of peroxide in the liquid sample, and the hydrogen peroxide contained in the liquid sample is treated differently in the first channel and the second channel to determine the reduction of hydrogen peroxide in one of the channels by the difference in the fluorescence intensity of the derivative liquid, thereby further determining the hydrogen peroxide concentration of the liquid sample. An advantageous improvement is that the first spiral tube and the third spiral tube are detachably connected and the second spiral tube and the fourth spiral tube are also detachably connected, so that when bubbles are observed in the solution in the hybrid double-layer spiral reaction chamber or any unexpected situation occurs, the operation can be stopped in time and the parts can be corrected or replaced to avoid equipment failure; in addition, the hybrid double-layer spiral reaction is also easier to maintain and transport, which increases its environmental applicability.

[0056] According to the single-layer spiral trap of the present application, Figure 2 As shown, it can be composed of only one spiral tube, i.e., the fifth spiral tube, and its spiral structure can improve the efficiency of capturing gas phase peroxide. In order to better realize the peroxide capture function, while taking into account the reduction of the volume of the device as much as possible, the length of the fifth spiral tube can be set between 40-100cm, ensuring that the gas phase peroxide can be fully absorbed after the absorption liquid forms an absorption liquid film on the inner wall of the tube and hits the absorption liquid film through molecular diffusion; the inner diameter of the fifth spiral tube can be set between 1.8-2.2mm, ensuring the efficient formation of the absorption liquid film and the aerodynamics of the gas phase peroxide. In order to reduce the occupied volume of the capture module, the spiral diameter of the fifth spiral tube can be set within the range of 15-20mm. When the spiral diameter is too large, the absorption liquid and the gas phase peroxide sample need more power to pass through the spiral tube, which will increase the use cost of the device.

[0057] According to a preferred implementation of the single-layer spiral trap of the embodiment of the present application, the inner diameter of the fifth spiral tube can be set to 2.2 mm, the spiral diameter can be set to 20 mm, and the number of turns of the spiral tube can be 15. The material of the fifth spiral tube is selected to be quartz glass, so that the resistance inside the tube can be relatively moderate, and the absorption liquid relies on the liquid tension to form a stable and thin liquid surface layer on the inner wall of the tube, thereby increasing the gas-liquid absorption interface.

[0058] In the present application, other components may also be configured for the single-layer spiral trap to help the single-layer spiral trap obtain air samples and further process the absorption liquid samples obtained by the single-layer spiral trap. The single-layer spiral trap according to the embodiment of the present application may also include one or more of an absorption liquid pump, a liquid flow controller, a gas three-way valve, or a gas-liquid separation unit for coordinated use.

[0059] In some possible embodiments, the upper end of the single-layer spiral trap can be connected to the gas three-way valve and the absorption liquid pump through independent pipelines, respectively. It can be understood that in practical applications, in order to perform baseline and / or standard material measurements on the device, the single-layer spiral trap can be connected to a gas three-way valve to control its switching between a sample gas collection mode for collecting atmospheric samples and a zero gas collection mode for collecting zero gas samples. In the zero gas collection mode, the zero gas sample enters the single-layer spiral trap through the gas three-way valve so as to perform a baseline zero point measurement through the hybrid double-layer spiral reaction chamber and any configurable fluorescence detection component. Specifically, after the zero gas sample is introduced, the single channel of the single-layer spiral trap, the dual channels of the hybrid double-layer spiral reaction chamber and / or the corresponding fluorescence detection components can be set to be consistent with the time series and program control in the sample gas collection mode. By measuring the zero point and / or standard substance of the zero gas sample, the reaction consistency in the dual channels when the various devices are used in combination can be obtained. The enzymatic hydrolysis and fluorescence reaction baselines of the hybrid double-layer spiral reaction chamber can also be corrected by drawing a calibration standard curve, so as to further improve the accuracy of hydrogen peroxide measurement using the single-layer spiral trap, the hybrid double-layer spiral reaction chamber and the corresponding components.

[0060] It should be noted that the flow rate and flow velocity of the absorption liquid need to be further balanced according to the size of the single-layer spiral trap to ensure that the absorption liquid film can form a good coverage and distribution on the inner wall of the spiral pipe. Too large a flow rate may cause the liquid film to be too thick or ruptured; too small a flow rate or too low a flow velocity may cause the liquid film to be incomplete or unevenly distributed. Therefore, a liquid flow meter can be further configured for the absorption liquid pump to accurately control the flow of the absorption liquid and continuously pump it into the single-layer spiral trap, thereby forming a stable absorption liquid film on the inner wall of the fifth spiral tube.

[0061] The absorption liquid sample obtained by using a single-layer spiral trap is usually transported to the reaction unit together with other gas phase components in the atmospheric sample. Therefore, in order to avoid gas interference, the lower end of the single-layer spiral trap can be first connected to a gas-liquid separation unit through a pipeline, and the captured liquid sample and the gas phase components that do not contain peroxides are transported to the gas-liquid separation unit for gas-liquid separation. After classification, the captured liquid sample is evenly divided into two parts and transported to the dual channels of the reaction module through pipelines.

[0062] In some possible embodiments, the absorption liquid containing peroxide is continuously entrained to move to the lower end of the single-layer spiral trap under the action of the fast-flowing atmosphere continuously introduced by the gas three-way valve, and the gas-liquid separation unit includes a gas-liquid separation chamber, a safety bottle, a filter membrane, a gas flow controller, an air pump and a degassing device; the absorption liquid leaves the spiral tube from the lower end and passes through the gas-liquid separation chamber, and the absorption liquid sample is drawn out from the bottom of the gas-liquid separation chamber through a propulsion component, such as a peristaltic pump, under the action of gravity and enters the degassing device, and the remaining atmosphere is discharged after passing through the safety bottle, the filter membrane, the flow controller and the air pump in sequence under the action of the air pump, and the discharge rate of these gases can be controlled in this process. In the degassing device, the absorption liquid sample removes the bubbles that are not completely dissolved due to gas-liquid separation in the spiral pipeline, and is further evenly divided into two paths A and B by the degassing device, and is respectively transported to the subsequent double channels, such as the first channel and the second channel for reaction under the action of the propulsion component, such as a peristaltic pump, i.e., the first reaction chamber and the second reaction chamber of the hybrid double-layer spiral reaction chamber according to the embodiment of the present application.

[0063] At present, the cooling of the capture trap and the heating of the reaction chamber are basically designed based on the technical idea of ​​attaching heating / cooling additional insulation cotton to the inner wall of the aluminum shell. This heating / cooling method transfers the temperature of the pipeline through the heating / cooling components on the inner wall, which cannot ensure the uniform and constant temperature of the upper and lower parts of the pipeline, and generally wraps the pipeline, so the fluid state in the pipeline cannot be observed in real time. In addition, this heating / cooling method is generally more suitable for metal pipelines, and has poor temperature transfer efficiency compared to glass materials with higher specific heat capacity. However, glass pipelines are generally used for the capture, transportation and reaction of hydrogen peroxide substances, which can keep the temperature distribution of the spiral pipeline uniform and make it convenient for the operator to observe the fluid running state in the pipe in real time.

[0064] Therefore, the present application designs a cooling tube that can directly contact the single-layer spiral trap made of quartz material that needs to be cooled for the single-layer spiral trap maintained at low temperature, and the cooling tube for fast temperature transfer is attached to the fifth spiral tube. One end of the cooling tube uses a refrigeration component, such as multiple refrigeration plates for cooling, and instantly transfers the set required temperature to the single-layer spiral trap to ensure uniform cooling of the single-layer spiral trap and avoid the reduction of capture efficiency caused by uneven temperature. Similarly, for the hybrid double-layer spiral reaction chamber maintained at high temperature, a heat conduction tube is designed to be in direct contact with the hybrid double-layer spiral quartz reaction chamber that needs to be heated, and multiple heaters, such as heat blocks, are connected and respectively contacted and placed from the upper end to the lower end of the hybrid double-layer spiral quartz reaction chamber to ensure uniform heating of the reaction chamber and avoid insufficient reaction caused by uneven heating of the reaction chamber and affect the detection signal strength.

[0065] Specifically, the single-layer spiral trap may further include a plurality of refrigeration components and a plurality of cooling pipes; wherein the plurality of refrigeration components are arranged at the lower end of the fifth spiral tube, and the plurality of cooling pipes extend from the plurality of refrigeration components along the central spiral axis of the fifth spiral tube in the middle and / or outer edge of the fifth spiral tube to the upper end of the fifth spiral tube, respectively, to keep the overall temperature of the fifth spiral tube constant. When the number of cooling pipes extending from the middle of the fifth spiral tube to the upper end of the fifth spiral tube along the central spiral axis of the fifth spiral tube is not less than 2, the cooling pipes extending from the middle of the fifth spiral tube to the upper end of the fifth spiral tube are evenly distributed, so as to help the single-layer spiral trap to be evenly cooled.

[0066] For a single-layer spiral capture trap, more cooling tubes and refrigeration components usually bring about a faster and more stable cooling effect, but the cost of using the equipment also increases accordingly; in some possible implementation schemes, the number of cooling tubes and refrigeration components that can be set for the fifth spiral tube can be between 3 and 8, so that the cooling tube can be quickly cooled and waste of refrigeration materials can be avoided. For example, 4 cooling tubes and corresponding refrigeration components can be evenly set on the outer edge of the fifth spiral tube, and 1 cooling tube and refrigeration component can be set in the middle of the fifth spiral tube, so that the cooling can be evenly conducted to the fifth spiral tube, and the cooling speed can also meet the time required for equipment startup in most detection situations; it can also be as shown in the attached Figure 2 As shown, a cooling tube is attached to the upper and lower edges of the fifth spiral tube, and a cooling tube is fixed in the middle, so as to minimize the cooling cost.

[0067] Similarly, the second reaction chamber of the hybrid double-layer spiral reaction chamber may also include multiple heaters and multiple heat pipes; the multiple heaters are arranged at the lower end of the second double-layer spiral tube, and the multiple heat pipes extend from the multiple heaters along the central spiral axis of the second double-layer spiral tube in the middle and / or outer edge of the second double-layer spiral tube to the upper end of the second double-layer spiral tube to keep the overall temperature of the second double-layer spiral tube constant; when the number of heat pipes extending from the middle of the second double-layer spiral tube to the upper end of the second double-layer spiral tube along the central spiral axis of the second double-layer spiral tube is not less than 2, the heat pipes extending from the middle of the second double-layer spiral tube to the upper end of the second double-layer spiral tube are evenly distributed to help the second reaction chamber to be heated evenly.

[0068] In some possible implementations, the number of heat-conducting tubes and heaters provided for the second double-layer spiral tube may be between 3 and 8, so that the heat-conducting tubes can transfer heat quickly and avoid wasting heating materials. For example, 4 heat-conducting tubes and corresponding heaters can be evenly arranged on the outer edge of the second double-layer spiral tube. For example, if the heat is fast, 1 heat-conducting tube and heater can be arranged in the middle of the second double-layer spiral tube, so that the heat can be evenly transferred to the second double-layer spiral tube. The heat transfer speed can also meet the time required for the equipment to start in most detection situations. Here, since the second double-layer spiral tube is arranged after the fifth spiral tube, the heating start-up time required can be more ample, so the required heat-conducting tubes can be less in the case of meeting stable heating. In addition, as shown in the attached Figure 1 As shown, only one heat conducting pipe is attached to the upper and lower edges of the second double-layer spiral tube, and one heat conducting pipe is fixed in the middle, so as to minimize the heating cost.

[0069] Based on the above-mentioned single-layer spiral trap and hybrid double-layer spiral reaction chamber, the present application provides a device for measuring the concentration of atmospheric hydrogen peroxide, which includes a trapping module, a reaction module, a detection module and a calculation module. Figure 3 As shown. The capture module is connected to the input end pipeline of the reaction module, and is used to inhale the test sample from the atmosphere and capture the peroxide in the test sample to obtain a capture liquid sample containing the test sample; the capture module divides the capture liquid sample into sample A and sample B and transports them to the reaction module respectively, processes them respectively in the reaction module and obtains two fluorescent reaction liquid samples through fluorescent reaction after processing; the detection module is connected to the output end pipeline of the reaction module, and is used to receive the two fluorescent reaction liquid samples and detect the total peroxide concentration in the two fluorescent reaction liquid samples respectively; the calculation module calculates the hydrogen peroxide concentration in the test sample based on the total peroxide concentration in the two fluorescent reaction liquid samples.

[0070] In some possible embodiments, the capture module includes an absorption liquid pump, a liquid flow controller, a gas three-way valve, a single-layer spiral capture trap as described in any one of claims 7 to 10, and a gas-liquid separation unit, wherein the upper end of the single-layer spiral capture trap is respectively connected to the gas three-way valve and the absorption liquid pump pipeline; the gas three-way valve introduces the test sample and / or the zero gas sample into the single-layer spiral capture trap; the absorption liquid pump introduces absorption liquid into the single-layer spiral capture trap and forms an absorption liquid film on the inner wall of the fifth spiral tube to capture peroxides in the test sample and obtain a capture liquid sample; the lower end of the single-layer spiral capture trap is connected to the gas-liquid separation unit pipeline, the gas-liquid separation unit performs gas-liquid separation on the capture liquid sample and the remaining gas, and divides the capture liquid sample into sample A and sample B to be transported to the reaction module respectively.

[0071] In some possible embodiments, the reaction module includes a hybrid double-layer spiral reaction chamber according to any one of claims 1 to 6; the first spiral tube obtains sample A and a parallel liquid respectively through a peristaltic pump and mixes sample A and the parallel liquid to obtain a mixed solution A, the second spiral tube obtains sample B and a reaction liquid respectively through a peristaltic pump and mixes sample B and the reaction liquid to obtain a mixed solution B; the third spiral tube obtains mixed solution A and a derivative liquid respectively from the lower end of the first spiral tube through a peristaltic pump and mixes the mixed solution A and the derivative liquid to obtain a fluorescent reaction liquid sample A, the fourth spiral tube obtains mixed solution B and a derivative liquid respectively from the lower end of the second spiral tube through a peristaltic pump and mixes the mixed solution B and the derivative liquid to obtain a fluorescent reaction liquid sample B.

[0072] In some possible embodiments, the detection module includes a fluorescence detection chamber for detecting fluorescent reaction liquid sample A and a fluorescence detection chamber for detecting fluorescent reaction liquid sample B; the fluorescence detection chamber for detecting fluorescent reaction liquid sample A and the fluorescence detection chamber for detecting fluorescent reaction liquid sample B are respectively connected to the lower end of the third spiral tube and the lower end of the fourth spiral tube.

[0073] The atmospheric hydrogen peroxide concentration measuring device according to the embodiment of the present application may also include a control module, which is electrically connected to one or more of the capture module, the reaction module, the detection module and the calculation module and is used to electrically control one or more of the capture module, the reaction module, the detection module and the calculation module. The control module can be exemplified by a single-chip microcomputer, a DSP, an FPGA, etc. In addition, it can be understood that in order to ensure that the atmospheric hydrogen peroxide concentration measuring device according to the embodiment of the present application normally uses liquid contents such as absorption liquid, liquid phase sample, reaction liquid and / or derivative liquid, liquid conveying components can be appropriately added according to actual application conditions to ensure the normal flow of the above-mentioned liquid contents in the measuring device, such as adding a peristaltic pump, a magnetic pump or a syringe, etc.

[0074] According to one aspect of the present application, a method for measuring hydrogen peroxide concentration is also provided, such as Figure 4 As shown, it may include the following steps:

[0075] Sampling process S110: capturing peroxides in the externally input sample to be tested, i.e. passing the sample to be tested through an absorption liquid so that the peroxides therein are absorbed by the absorption liquid, and obtaining a captured liquid containing the sample to be tested; reaction process S120: dividing the processed sample to be tested obtained after gas-liquid separation of the captured liquid into sample A and sample B on average, and reacting them separately to obtain a dyeing reaction liquid, i.e. sample A reacts with a derivative liquid for fluorescence reaction, sample B reacts with catalase to remove hydrogen peroxide and then reacts with the derivative liquid for fluorescence reaction to obtain dyeing reaction liquids respectively; detection process S130: based on the dyeing reaction liquid, detecting the total peroxide concentration P in sample A and detecting the peroxide concentration Q in sample B after removing hydrogen peroxide; calculation process S140: calculating the concentration M of hydrogen peroxide in the sample to be tested, wherein M=(PQ).

[0076] In some preferred embodiments, the measurement method may further include performing a zero gas test on the pipeline before measuring the sample to be tested to verify the parallelism of the pipeline and / or calibrate the pipeline, such as Figure 5 As shown, it may include the following steps: Step S150: Take zero gas as the sample to be detected and pass it into the pipeline where the absorption liquid is located to obtain zero gas capture liquid; Step S160: After the zero gas capture liquid is subjected to gas-liquid separation, the processed sample to be detected is evenly divided into sample A' and sample B', and reacted separately to obtain a dye reaction liquid, that is, sample A' reacts with the derivative liquid for fluorescence reaction, and sample B' reacts with catalase and then reacts with the derivative liquid for fluorescence reaction; Step S170: Detect the total peroxide concentration P' in sample A' and the concentration Q' of peroxide after removing hydrogen peroxide from sample B'; Step S180: Calculate the concentration M' of hydrogen peroxide in the sample to be detected, where M'=(P'-Q'). In this way, it can be detected whether the pipeline corresponding to sample A' and the pipeline corresponding to sample B' are consistent, and the zero gas can be selected from high-purity nitrogen or compressed air.

[0077] That is, the concentration of peroxide in sample A' and sample B' is 0, so the concentration P' and the concentration Q' should also be 0 or equal, and the final concentration M' should also be 0. If the concentration M' is not 0, the hydrogen peroxide detection baselines of the two pipelines are different, and the contaminated pipeline can be determined based on the value of the concentration M', so that the technicians can clean or replace it to ensure the parallelism of the two pipelines.

[0078] It can be understood that the method for measuring the concentration of hydrogen peroxide described above, and its detection process corresponds to the use of the measuring device for the concentration of atmospheric hydrogen peroxide described above, including its capture module, reaction module, detection module and calculation module. The capture module is connected to the pipeline of the reaction module, and is used to inhale the sample gas to be detected from the outside, and to capture the peroxide in the sample gas to be detected, and obtain a capture liquid containing the detection sample; the reaction module is respectively connected to the pipelines of the capture module and the detection module, and the capture module is evenly divided into two channels after gas-liquid separation to enter the reaction module and perform fluorescence reactions respectively, and obtain two different fluorescent reaction liquids; the detection module adopts dual-channel detection, and is used to receive two different fluorescent reaction liquids, and respectively detect the total peroxide concentration P in the sample and the concentration Q of the peroxide after removing hydrogen peroxide from the detection sample, and the calculation module is used to calculate the concentration M of hydrogen peroxide in the detection sample, where M=(PQ).

[0079] In particular, the capture module includes the single-layer spiral capture trap; the reaction module includes the hybrid double-layer spiral reaction chamber. The detection module includes a fluorescence detection chamber for detecting P and a fluorescence detection chamber for detecting Q, wherein the fluorescence detection chamber for detecting P is connected to the pipeline corresponding to sample A, and the fluorescence detection chamber for detecting Q is connected to the pipeline corresponding to sample B.

[0080] The integrated capture reaction-integrated atmospheric hydrogen peroxide concentration measuring device provided by the present application maximizes the common parts of the two capture reaction pathways, increases the sensitivity of the device, reduces the number of parts used in the device and the volume, so as to improve the portability of the device. The present application designs a single-layer single-layer spiral capture trap in the measuring device, inserts the cooling tube into multiple positions such as the upper end, middle end and lower end of the spiral tube of the single-layer spiral capture trap, uses the cooling tube as a cooling component, connects the refrigeration component at the tail end of the cooling tube, so as to quickly transfer the low temperature to the entire end of the cooling tube, and evenly cools the single-layer spiral capture trap in real time, maintaining a constant low temperature from upstream to downstream of the single-layer spiral capture trap, which can reduce the refrigeration volume and avoid the capture efficiency being affected by uneven low temperature.

[0081] In addition, the present application designs the reaction chamber for dual-channel detection in the same reaction unit, which simplifies the gas path and reduces the volume. A high-temperature device can be used to keep the dual-channel hybrid double-layer spiral reaction chamber in a constant high temperature state, ensuring that it is heated evenly, avoiding the uneven temperature of the hybrid double-layer spiral reaction chamber causing insufficient reaction and affecting the detected signal strength. After multiple tests and improvements, the design of the entire measuring device is small in size and compact in structure, and can perform online continuous monitoring of hydrogen peroxide in a variety of operating scenarios.

[0082] Example

[0083] This application provides a general and / or specific description of the materials and test methods used in the experiment. The raw materials or instruments used without indicating the manufacturer are all conventional raw materials or instruments that can be obtained through commercial purchase.

[0084] Example 1

[0085] The composition structure of the atmospheric hydrogen peroxide concentration measuring device is as follows: Figure 3 As shown, the device is used to monitor the hydrogen peroxide content in the ambient air online, and the specific implementation method is as follows:

[0086] Under the action of the first peristaltic pump 13, the absorption liquid is stably and continuously transported into the single-layer spiral trap 23 of the single-layer low-temperature capture module 2 through the precise flow control of the liquid flow controller 1, so as to fully wet the inner wall of the single-layer spiral trap 23 and form an absorption liquid surface layer on the inner wall of the single-layer spiral trap 23. When analyzing the atmospheric sample, the sample gas, i.e., the atmospheric sample, enters the low-temperature maintained single-layer spiral trap 23 through the gas three-way valve 12 under the suction of the air pump 7. At a constant temperature, the gas phase peroxide impacts the absorption liquid surface layer on the inner wall of the trap due to molecular diffusion and is fully absorbed.

[0087] The liquid sample after absorbing the peroxide in the atmospheric sample to be tested is continuously carried to the downstream of the single-layer spiral trap 23 by the fast-flowing air in the atmospheric sample, until it reaches the gas-liquid separation unit 3 under the action of the second peristaltic pump 14. In the gas-liquid separation unit 3, the liquid sample after absorbing the peroxide to be tested is drawn out from the bottom by the third peristaltic pump 15 under the action of gravity and enters the debubbling device 8, and the remaining air in the atmospheric sample is safely discharged after passing through the safety bottle 4, the filter membrane 5, and the gas flow controller 6 in sequence under the action of the air pump 7.

[0088] After absorbing the substance to be tested, the liquid sample passes through the debubbling device 8 to remove the bubbles in the pipeline caused by incomplete gas-liquid separation, and is evenly divided into two paths A and B to form sample A and sample B, and enter the first channel and the second channel of the hybrid double-layer spiral reaction chamber 9 of the reaction module respectively. The liquid sample first enters the first double-layer spiral tube in the hybrid double-layer spiral reaction chamber 9, and the deionized water and the catalase solution enter the first channel and the second channel respectively under the action of the fourth peristaltic pump 16 and the fifth peristaltic pump 17, wherein the catalase solution in the second channel is mixed with the liquid sample in the second spiral tube, and hydrogen peroxide is rapidly decomposed under the action of catalase. Therefore, the hydrogen peroxide in the liquid sample solution of the second channel is quickly consumed, while the hydrogen peroxide in the liquid sample solution of the first channel is retained, and the solutions in the two channels then enter the second double-layer spiral tubes of the two channels.

[0089] At the same time, the derivative liquid also enters the second double-layer spiral tube under the action of the sixth peristaltic pump 18 and reacts with the peroxide in the liquid sample solution at a constant temperature of 40°C. The derivative liquid in the third spiral tube of the first channel reacts with the total peroxide, and the derivative liquid in the fourth spiral tube of the second channel reacts with the peroxide in the liquid sample solution after the hydrogen peroxide is removed. After the reaction, the liquid sample solutions of the two channels generate fluorescent samples downstream in the flow direction and simultaneously enter the subsequent detection module 10 for measurement of the intensity of the fluorescent signal. The accurate content of hydrogen peroxide decomposed by catalase in the liquid sample solution in the second channel is determined by the difference in the intensity of the fluorescent signals of the first channel and the second channel, and then the concentration of hydrogen peroxide in the atmospheric sample can be obtained.

[0090] Among them, the single-layer spiral trap 23 at low temperature and the hybrid double-layer spiral reaction chamber 9 at high temperature start working when the device is turned on, and keep constant low / high temperature. The liquid flow controller 1, the single-layer spiral trap 23, the gas flow controller 6, the gas pump 7, the hybrid double-layer spiral reaction chamber 9, the detection modules 10 and 11, the gas three-way valve 12, and the first peristaltic pump to the sixth peristaltic pump 13-18 are all controlled in real time by the control module 19 to realize the time series control of the entire device and each component, and provide a solution for real-time and continuous atmospheric hydrogen peroxide monitoring.

[0091] When performing baseline and standard material measurements, the gas three-way valve 12 is switched to zero gas mode, and the absorption liquid, deionized water, catalase and derivative liquid enter the single-layer spiral trap 23 at low temperature and the mixed double-layer spiral reaction chamber 9 at high temperature respectively under the control of each peristaltic pump, for absorption and reaction respectively. The zero gas sample enters the device through the gas three-way valve 12 for the process of baseline zero point measurement, that is, the time series control process of the sampling step, the reaction step and the detection step is consistent with the step of measuring the atmospheric sample, and the zero point hydrogen peroxide content of the first channel and the second channel can be obtained. In this way, the consistency of the reaction and detection baseline of the two channels can be confirmed, and the calibration standard curve can be drawn, etc., so as to accurately calibrate the device, improve the parallelism of the reaction of the two channels, and improve the accuracy of atmospheric hydrogen peroxide monitoring.

[0092] Example 2

[0093] The capture module 2 includes a quartz glass single-layer spiral capture trap 23 for absorbing atmospheric hydrogen peroxide and peroxide and its related components for maintaining low temperature, including a refrigeration component 24, a cooling pipe 21 and an additional radiator 25. The front-end capture is integrated, and only a single-channel spiral capture trap is needed to meet the capture requirements, such as Figure 2 and Figure 3After testing, the inner diameter of the single-layer spiral trap set this time is 2.2mm, the spiral diameter is 20mm, the number of windings is 15, the resistance inside the trap is moderate, and the absorption liquid relies on liquid tension to form a thin absorption liquid surface layer on the inner wall of the trap, which can increase the gas-liquid absorption interface with the atmospheric sample.

[0094] At a certain low temperature capture temperature, the saturated vapor pressure of peroxides decreases, making them easier to be captured by the absorption liquid, which can improve the enrichment efficiency. In addition, it also reduces the volume effect of air on the liquid, making the subsequent determination of peroxide concentration more accurate. After testing, setting the capture temperature to 5°C can ensure a capture efficiency of 100%, and also minimize the absorption liquid carried away by excess airflow.

[0095] Since the single-layer spiral trap is designed as a multi-turn spiral structure, simple refrigeration components such as cooling plates cannot cool it evenly, which can easily lead to uneven temperature inside the tube.

[0096] In order to effectively control the constant low temperature of the single-layer spiral trap and reduce the volume of refrigeration-related components, considering the efficient and fast cooling performance of the cooling tube, three cooling tubes 21 are fixed in parallel to the central axis and two sides of the single-layer spiral trap 23 along the sample flow direction, so that the three cooling tubes 21 as a whole are fully in contact with various parts of the single-layer spiral trap 23, wherein the cooling tube 21 located at the central axis and one side can ensure that the temperature of one side of the single-layer spiral trap 23 remains uniform, and the cooling tube 21 located at the central axis and the other side can ensure that the temperature of the other side of the single-layer spiral trap 23 remains uniform. The three cooling tubes 21 are connected to the semiconductor refrigerator 24 disposed downstream of the single-layer spiral trap 23 to provide continuous cooling.

[0097] Thus, the three cooling tubes 21 work together to ensure that the temperature in each location of the single-layer spiral trap 23 is uniform and constant, and a thermally conductive silicone grease layer is used to tightly fix the connection ends of the three cooling tubes 21 and the semiconductor cooling sheet 24. The hot end of the semiconductor cooling sheet 24 is connected to the copper tube radiator 25 through the thermally conductive silicone grease layer, thereby ensuring the continuous and efficient operation of the cooling sheet.

[0098] In addition, in order to monitor the actual temperature in the trap in real time, a patch temperature sensor 22 is used, which is attached to the outer wall of the single-layer spiral trap 23 in the middle of the sample flow direction. Since the single-layer spiral trap 23 is centrally symmetrical at this position and the thermal conductivity of quartz glass is good, the temperature sensor is set here to ensure that it monitors the actual average temperature in the single-layer spiral trap 23. This avoids large errors in temperature monitoring, which leads to uneven temperature in the tube. Such a setting is simple and efficient. By electrically connecting the patch temperature sensor 22 to the control module 19, the capture temperature can be monitored in real time through the control module 19. When necessary, the operation can be stopped or the temperature of the capture module 2 can be adjusted to ensure that the sampling process strictly follows the set temperature parameters to maintain a high capture efficiency.

[0099] Example 3

[0100] The reaction module 9 includes a second double-layer spiral tube for contacting the substance to be tested with the derivatization reagent, that is, the derivatization liquid of the fluorescence reaction to generate the fluorescent substance, and related components for maintaining the high temperature, such as Figure 1 and Figure 3 As shown. In the reaction stage, the channel needs to be evenly divided into two pipelines for separate reactions. In order to simplify the gas path and the device, this embodiment sets two spiral tubes that are alternately arranged in parallel up and down as the third channel and the fourth channel to form a second double-layer spiral tube 29 as a reaction chamber. The volume of the two channels is reduced by overlapping the structure, that is, only one channel area is occupied in the axial direction, and the length is significantly shortened. The residence time of the solution in the second double-layer spiral tube will affect the degree of fluorescent derivatization reaction. After testing, the inner diameter of the two channels set this time is 1.5mm, the spiral diameter is 25mm, the number of windings is 15, and the material is quartz glass, which can ensure sufficient derivatization reaction.

[0101] The second double-layer spiral tube is used as a reaction chamber. Its internal temperature affects both the reaction rate and the fluorescence quantum yield of the fluorescent substance. In order to achieve the best reaction and fluorescence effect, the reaction chamber needs to be controlled at a certain temperature. After testing, 35℃ was selected as the reaction temperature of the reaction chamber. In order to simplify the related components of high-temperature control and ensure the uniform and constant temperature in the tube, this time, copper heat pipes with high thermal conductivity, long life and low unit price are used as heat pipes to contact and heat the glass tube. Three heat pipes 26 are evenly placed at the upper, middle and downstream positions of the second double-layer spiral tube. A heating block 27 with a heating power of 50W is placed at one end of the heat pipe 26, such as the rear end. The heat generated by the heating block 27 is quickly transferred to the heat pipe 26, and the heat is transferred to the tube through the heat pipe 26 with a large surface area. The upstream and midstream heat pipes can ensure the uniform temperature of the upper half of the second double-layer spiral tube, and the midstream and downstream heat pipes can evenly heat the lower half of the second double-layer spiral tube to ensure its constant and uniform temperature. The three heat pipes work together to ensure the uniform and constant temperature in the spiral tube.

[0102] In addition, in order to monitor the actual temperature in the tube in real time, a patch temperature sensor 28 is used and attached to the middle position of the second double-layer spiral tube. Its symmetrical structure can ensure that the actual temperature in the tube is monitored, avoiding temperature monitoring errors that lead to uneven temperature in the tube. This setting is simple and efficient, and can ensure the uniformity of the temperature of the second double-layer spiral tube, meeting the reaction temperature while ensuring the feasibility and comparability of this double-layer pipeline. The following table shows the time required for the reaction temperature to produce a fluorescent reaction in the reaction chamber when different numbers of heat transfer tubes are set:

[0103] Table 1 Reaction temperature start-up time of hybrid double-layer spiral reactor with different numbers of heat pipes

[0104] Number of heat pipes Heat transfer rate (℃ / s) Time required to reach 35℃(s) 2 7 5 3 11.7 3 5 23.3 1.5 8 35 1 12 50 0.7

[0105] It can be seen that the number of heat pipes is positively correlated with the time required to reach the reaction temperature. It only takes 1.5 seconds for 5 heat pipes to heat the second reaction chamber of the double-layer spiral reaction chamber, which reduces the startup delay time of the equipment and maximizes the use efficiency of the equipment. In this embodiment, the second channel is used to decompose the hydrogen peroxide in the absorption liquid, and the hydrogen peroxide in the absorption liquid is not processed in the first channel. First, a catalase solution is added to the second spiral tube of the second channel, and the fluorescence signal intensity of the mixed solution of the absorption liquid and the catalase solution is tested. Multiple tests are performed and the average value is taken. The results are shown in Table 2.

[0106] Table 2 Effect of different parameters of the second spiral tube on the fluorescence detection effect of standard hydrogen peroxide solution of the measuring device

[0107]

[0108]

[0109] It can be seen from the above that the hydrogen peroxide in the absorption liquid is completely decomposed after the length of the second spiral tube reaches 60cm. In order to minimize the occupied volume of the second spiral tube, the number of spiral windings is set to 9. Increasing the number of turns will increase the equipment volume and measurement time, reduce the reaction efficiency and increase the manufacturing cost of the device. The compressed pitch will reduce the turbulence intensity in the spiral tube, making the mixed flow of the absorption liquid and the catalase solution in the tube more stable, which causes part of the solution to be engulfed by the low-velocity turbulence and unable to effectively contact the tube wall for heating; in addition, the tube wall itself is more difficult to be heated from the heat-conducting tubes that are fitted around it due to the reduction of gaps. Therefore, it is preferred that the length of the second spiral tube is 60cm and the number of windings is 9.

[0110] After determining the number of winding turns under the optimal length, the size of the inner diameter of the spiral tube was simulated and tested. It was found that when the inner diameter of the spiral tube was increased to 1.1 mm, the efficiency of hydrogen peroxide enzymatic hydrolysis was maximized. This is because the inner surface liquid film of the second spiral tube made of quartz glass is large and the contact area between the solutions is large, which is conducive to the full liquid-liquid reaction of hydrogen peroxide and catalase therein. Further increasing the inner diameter, it was found that the hydrogen peroxide detection sensitivity of the device was reduced, which was caused by the strong longitudinal diffusion of the solution in the second spiral tube. In this way, after determining the length of the second spiral tube, the optimal number of winding turns (9 turns) and the optimal inner diameter (1.1 mm) were obtained by controlling the variables, so that the volume of the second spiral tube was minimized as much as possible to ensure the efficient decomposition efficiency of hydrogen peroxide therein, thereby effectively providing support for subsequent difference detection. It can be understood that the first spiral tube can also be set to the same structure, and the first spiral tube and the second spiral tube can replace each other.

[0111] On this basis, the spiral tube parameters of the subsequent fluorescence reaction were further set, multiple tests were performed and the average value was taken. The test results are shown in the following table.

[0112] Table 3 Effect of different parameters of the third spiral tube on the fluorescence detection effect of standard hydrogen peroxide solution of the measuring device

[0113]

[0114]

[0115] When the length of the third spiral tube reaches 120 cm, it can ensure that the derivative solution and the peroxide react completely in the shortest time and reach the maximum detection fluorescence signal value, reducing the volume of the equipment and the delay time. Similarly, it is preferred that the number of windings of the third spiral tube is 13. Too high a number of windings may affect the sufficient heating of the two reaction solutions, and it is necessary to limit the pitch to increase the turbulence intensity and improve the heat transfer efficiency.

[0116] After determining the number of winding turns under the optimal length, the inner diameter of the spiral tube was also simulated and tested, and it was found that the reaction efficiency reached the maximum when the inner diameter of the spiral tube was increased to 1.5mm. This is because the inner surface liquid film of the third spiral tube made of quartz glass material is larger and the contact area between the solutions is large, which is conducive to the derivative liquid and peroxide to fully carry out liquid-liquid reaction therein. Further increase the inner diameter and find that the peroxide detection sensitivity of the device is reduced. When the length of the preferred third spiral tube is 120cm, the number of winding turns of the further preferred third spiral tube is 13 turns and the inner diameter is 1.5mm. It can be understood that the fourth spiral tube can also be set to the same structure, and the third spiral tube and the fourth spiral tube can replace each other.

[0117] In this way, the preferred size configuration of the first channel and the second channel and the length / pitch ratio and length / inner diameter ratio under the specified minimum available reaction length are obtained, so that the volume of the reaction chamber can be reduced to a minimum, while maximizing the reduction of the delay time of the equipment and ensuring the parallelism and efficiency of the reactions in the two channels, so that the atmospheric hydrogen peroxide concentration measurement device provided by the present application has a lower cost and a wider practical application range, so as to monitor the atmospheric hydrogen peroxide in real time.

Claims

1. A hybrid double-layer spiral reaction chamber, comprising: A first reaction chamber and a second reaction chamber are arranged in sequence in the injection direction, wherein the first reaction chamber comprises a first double-layer helical tube composed of a first helical tube and a second helical tube arranged intertwined with each other, and the second reaction chamber comprises a second double-layer helical tube composed of a third helical tube and a fourth helical tube arranged intertwined with each other; The first spiral tube and the third spiral tube are detachably connected to form a first channel for detecting the concentration of peroxide in the test sample; as well as The second spiral tube and the fourth spiral tube are detachably connected to form a second channel for detecting the peroxide concentration in the test sample.

2. The hybrid double-layer spiral reaction chamber according to claim 1, wherein: The second reaction chamber includes a plurality of heaters and a plurality of heat conducting pipes; The plurality of heaters are arranged at the lower end of the second double-layer spiral tube, and the plurality of heat-conducting tubes extend from the plurality of heaters along the central spiral axis of the second double-layer spiral tube in the middle and / or outer edge of the second double-layer spiral tube to the upper end of the second double-layer spiral tube, so as to keep the overall temperature of the second double-layer spiral tube constant; When the number of heat-conducting pipes extending from the middle of the second double-layered spiral tube to the upper end of the second double-layered spiral tube along the central spiral axis of the second double-layered spiral tube is not less than 2, the heat-conducting pipes extending from the middle of the second double-layered spiral tube to the upper end of the second double-layered spiral tube are evenly distributed; The heater makes the temperature of the second reaction chamber be 30-45°C.

3. The hybrid double-layer spiral reaction chamber according to claim 1, wherein: The first spiral tube and the second spiral tube are made of quartz glass or polytetrafluoroethylene; The inner diameters of the first spiral tube and the second spiral tube are 1-1.2 mm; The lengths of the first spiral tube and the second spiral tube are 30-90 cm; The number of winding turns of the first spiral tube and the second spiral tube is 6-10 turns; and The spiral diameter of the first spiral tube and the second spiral tube after winding is 10-15 mm.

4. The hybrid double-layer spiral reaction chamber according to claim 1, wherein: The third spiral tube and the fourth spiral tube are made of quartz glass or polytetrafluoroethylene; The inner diameters of the third spiral tube and the fourth spiral tube are 1.2-1.5 mm; The lengths of the third spiral tube and the fourth spiral tube are 90-130 cm; The number of winding turns of the third spiral tube and the fourth spiral tube is 10-15 turns; and The spiral diameter of the third spiral tube and the fourth spiral tube after winding is 15-20 mm.

5. The hybrid double-layer spiral reaction chamber according to claim 2, wherein: The number of the plurality of heat conducting pipes is 3-8; Preferably, the number of the plurality of heat conducting pipes is 8; The material of the plurality of heat pipes is one or two or more of pure copper, brass, combined heat pipe or nickel-chromium heating wire; and The length of the heat-conducting pipe is 120-150 cm, and the width of the heat-conducting pipe is 1-3 mm.

6. The hybrid double-layer spiral reaction chamber according to claim 2, wherein: The material of the multiple heaters is one, two or three of copper, aluminum and stainless steel; The second reaction chamber also includes a patch temperature sensor.

7. A single-layer spiral trap, comprising: A fifth spiral tube, a plurality of refrigeration components and a plurality of cooling pipes, wherein the plurality of refrigeration components are arranged at the lower end of the fifth spiral tube, and the plurality of cooling pipes extend from the plurality of refrigeration components along the central spiral axis of the fifth spiral tube in the middle and / or outer edge of the fifth spiral tube to the upper end of the fifth spiral tube, so as to keep the overall temperature of the fifth spiral tube constant; When the number of cooling tubes extending from the middle of the fifth spiral tube to the upper end of the fifth spiral tube along the central spiral axis of the fifth spiral tube is not less than 2, the cooling tubes extending from the middle of the fifth spiral tube to the upper end of the fifth spiral tube are evenly distributed; The refrigeration component makes the temperature of the fifth spiral tube be 0-10°C.

8. The single-layer spiral trap according to claim 7, wherein: The inner diameter of the fifth spiral tube is 1.8-2.2 mm; The length of the fifth spiral tube is 40-100 cm; The fifth helical tube has 10-16 turns; and The spiral diameter of the fifth spiral tube is 15-20 mm.

9. The single-layer spiral trap according to claim 7, wherein: The number of the plurality of cooling pipes is 3-8; Preferably, the number of the plurality of heat conducting pipes is 8; The material of the plurality of cooling pipes is pure copper and / or brass; and The length of the cooling tube is 120-150 cm, and the width of the cooling tube is 1-3 mm.

10. The single-layer spiral trap according to claim 7, wherein: The material of the multiple refrigeration components is one, two or three of brass, pure copper and aluminum; The single-layer spiral trap also includes a patch-type temperature sensor.

11. A device for measuring atmospheric hydrogen peroxide concentration, comprising: Capture module, reaction module, detection module and calculation module; The capture module is connected to the input end pipeline of the reaction module, and is used to inhale the test sample from the atmosphere and capture the peroxide in the test sample to obtain a capture liquid sample containing the test sample; the capture module divides the capture liquid sample into sample A and sample B and transports them to the reaction module respectively, processes them respectively in the reaction module and obtains two fluorescent reaction liquid samples through fluorescent reaction after the processing; The detection module is connected to the output end pipeline of the reaction module, and is used to receive the two fluorescent reaction liquid samples and respectively detect the total peroxide concentration in the two fluorescent reaction liquid samples; The calculation module calculates the concentration of hydrogen peroxide in the test sample based on the total concentration of peroxide in the two fluorescent reaction liquid samples.

12. The device for measuring atmospheric hydrogen peroxide concentration according to claim 11, wherein: The capture module includes an absorption liquid pump, a liquid flow controller, a gas three-way valve, a single-layer spiral capture trap according to any one of claims 7 to 10, and a gas-liquid separation unit, wherein: The upper end of the single-layer spiral trap is respectively connected to the gas three-way valve and the absorption liquid pump pipeline; The gas three-way valve introduces the test sample and / or the zero gas sample into the single-layer spiral trap; The absorption liquid pump introduces the absorption liquid into the single-layer spiral trap and forms an absorption liquid film on the inner wall of the fifth spiral tube to capture the peroxide in the test sample and obtain a capture liquid sample; The lower end of the single-layer spiral trap is connected to the gas-liquid separation unit pipeline, and the gas-liquid separation unit performs gas-liquid separation on the captured liquid sample and the remaining gas, and divides the captured liquid sample into sample A and sample B to be transported to the reaction module respectively.

13. The device for measuring atmospheric hydrogen peroxide concentration according to claim 11, wherein: The reaction module comprises a hybrid double-layer spiral reaction chamber according to any one of claims 1 to 6; The first spiral tube obtains the sample A and the parallel solution respectively through a peristaltic pump and mixes the sample A and the parallel solution to obtain a mixed solution A, and the second spiral tube obtains the sample B and the reaction solution respectively through a peristaltic pump and mixes the sample B and the reaction solution to obtain a mixed solution B; The third spiral tube obtains the mixed solution A and the derivative solution from the lower end of the first spiral tube respectively through a peristaltic pump and mixes the mixed solution A and the derivative solution to obtain a fluorescent reaction solution sample A; The fourth spiral tube obtains the mixed solution B and the derivative solution from the lower end of the second spiral tube respectively through a peristaltic pump and mixes the mixed solution B and the derivative solution to obtain a fluorescent reaction solution sample B.

14. The device for measuring atmospheric hydrogen peroxide concentration according to claim 11, wherein: The detection module includes a fluorescence detection chamber for detecting the fluorescence reaction liquid sample A and a fluorescence detection chamber for detecting the fluorescence reaction liquid sample B; The fluorescence detection chamber for detecting the fluorescence reaction liquid sample A and the fluorescence detection chamber for detecting the fluorescence reaction liquid sample B are connected to the lower end of the third spiral tube and the lower end of the fourth spiral tube respectively.

15. A method for measuring hydrogen peroxide concentration, comprising the following steps: Sampling process: capturing peroxides in the sample to be tested input from the outside, passing the sample to be tested through an absorption liquid so that the peroxides therein are absorbed by the absorption liquid, and obtaining a capture liquid containing the sample to be tested; Reaction process: the processed sample to be detected obtained after gas-liquid separation of the captured liquid is evenly divided into sample A and sample B, and the two samples are reacted to obtain a dyeing reaction liquid; Detection process: Based on the dyeing reaction solution, the total peroxide concentration P in the detection sample A and the peroxide concentration Q after removing hydrogen peroxide in the detection sample B are detected; Calculation process: Calculate the concentration M of hydrogen peroxide in the sample to be tested, where M = (PQ); Preferably, the measuring method further comprises the following steps: A calibration process is performed before measurement. During calibration, zero gas is used as the sample gas to be measured, and the sampling process, reaction process, and detection process are performed to see whether the pipeline corresponding to sample A and the pipeline corresponding to sample B are consistent. The zero gas is high-purity nitrogen or compressed air.

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