Recombinant jellyfish thioredoxin cream as well as preparation method and application thereof

Abstract

The invention relates to the field of biological medicine, in particular to recombinant jellyfish thioredoxin cream as well as a preparation method and application thereof. The emulsifiable paste is prepared by mixing recombinant jellyfish thioredoxin with efficient transmembrane capacity and remarkable antioxidant activity as a main component with an emulsifiable paste matrix. The adverse symptoms such as skin dryness, desquamation and wrinkles damaged by ultraviolet radiation can be obviously relieved, and the epidermal hyperplasia and skin oxidative stress level can be improved. The jellyfish-derived antioxidant is externally applied to skin ultraviolet radiation injury protection for the first time, a new medicine source is provided for preventing skin ultraviolet radiation injury, and a new application field is expanded for jellyfish biological resource development.

Description

Technical Field

[0001] The invention relates to the field of biomedicine, and in particular to a recombinant jellyfish thioredoxin cream and a preparation method and application thereof. Background Art

[0002] Excessive ultraviolet radiation can lead to an increase in the number of active oxygen free radicals in skin cells. Exogenous antioxidants can enhance the skin's antioxidant capacity and effectively protect against ultraviolet-induced skin damage. Currently, the antioxidants commonly used in anti-ultraviolet radiation skin care products are mainly derived from artificial synthesis or natural plant extracts. Some of these antioxidants may have adverse pro-oxidative effects due to their different structures, dosages, and environments. Therefore, it is necessary to find more effective and safe natural antioxidants to improve the effectiveness and safety of anti-ultraviolet skin care products.

[0003] Marine plankton has strong resistance to ultraviolet radiation due to its adaptation to sunlight, and may become a new source of anti-ultraviolet radiation and antioxidant active substances. Jellyfish are a type of large invertebrate marine plankton with very rich resources. A variety of antioxidant substances with significant activity have been isolated from jellyfish. Among them, thioredoxin (Trx), as one of the important endogenous antioxidant molecules, has been proven to have excellent antioxidant activity and plays an important role in regulating physiological processes such as cell oxidative stress and cell apoptosis.

[0004] It is difficult for exogenous biomacromolecules such as proteins and peptides to directly enter cells to exert their effects. Protein transduction domain (TAT-PTD), also known as cell-penetrating peptide, is a short peptide containing 5 to 30 amino acid residues that can effectively carry various biomacromolecules into cells with fast transduction speed and high efficiency. At present, there are no reports on the external use of jellyfish-derived antioxidants for the skin to resist ultraviolet radiation. Summary of the invention

[0005] The purpose of the present invention is to provide a recombinant aequorea thioredoxin cream for protecting skin from ultraviolet radiation damage and a preparation method thereof. The present invention develops an ointment with recombinant aequorea thioredoxin having high transmembrane capacity and significant antioxidant activity as a main component and a preparation method thereof, and clarifies its protective effect on skin ultraviolet radiation damage, providing a new resource for the development of safer and more efficient natural anti-ultraviolet radiation protection drugs.

[0006] The first aspect of the present invention provides a recombinant aequorea thioredoxin cream, which is prepared by using recombinant aequorea thioredoxin (PTD-CcTrx1) as a main active ingredient and mixing it evenly with a cream base; the amino acid sequence of the recombinant aequorea thioredoxin (PTD-CcTrx1) is shown in SEQ ID NO:3.

[0007] The recombinant jellyfish thioredoxin (PTD-CcTrx1) was obtained by the inventors in the early stage through recombinant cloning expression, and has significant antioxidant activity and efficient transmembrane ability (reference: Wang B, Zhang P, Wang Q, et al. Protective effects of a jellyfish-derived thioredoxin fused with cell-penetrating peptide TAT-PTD on H2O2-induced oxidative damage. 2023, 24, 7340).

[0008] The recombinant jellyfish thioredoxin is obtained by combining the transmembrane peptide (TAT-PTD) gene coding sequence (SEQ ID NO: 1) with the hair-shaped jellyfish thioredoxin (CcTrx1) gene coding sequence (SEQ ID NO: 2) through gene recombination technology, connecting the fusion coding sequence to a vector using an enzyme cutting site, and inducing expression and purification. The amino acid sequence of the recombinant jellyfish thioredoxin (PTD-CcTrx1) is shown in SEQ ID NO: 3, and the nucleotide coding sequence of the recombinant protein PTD-CcTrx1 is shown in SEQ ID NO: 4.

[0009] The present invention uses an oil-in-water (O / W) cream matrix type, which can make the recombinant jellyfish thioredoxin stock solution dissolve well in the matrix, facilitate skin absorption, and increase the efficacy of the drug. The oil phase material of the cream matrix is ​​composed of a mixture of five substances: stearic acid, Span-80, octadecyl alcohol, liquid paraffin, and white vaseline, and the water phase material is composed of a mixture of four substances: polysorbate 80, glycerol, sorbic acid, and deionized water.

[0010] Furthermore, the amount of recombinant aequorea thioredoxin added to the cream is 0.5% to 2% relative to the mass of the cream matrix.

[0011] Furthermore, the mass percentage of each raw material in the cream base is 6% to 8% of stearic acid, 1.6% to 2% of Span-80, 6% to 8% of octadecyl alcohol, 9% to 10% of liquid paraffin, 6% to 8% of white vaseline, 4% to 5% of polysorbate 80, 10% to 12% of glycerol, 0.2% to 0.4% of sorbic acid, and the balance is deionized water.

[0012] The second aspect of the present invention provides a method for preparing the recombinant jellyfish thioredoxin cream as described above, comprising the following steps:

[0013] S1: Preparation of recombinant jellyfish thioredoxin stock solution: The recombinant jellyfish thioredoxin (PTD-CcTrx1) gene coding sequence as shown in SEQ ID NO: 4 was obtained by chemical synthesis, and the two ends of the sequence had specific restriction sites (NdeI: 5'-CATATG-3', XhoI: 5'-CTCGAG-3'), and the recombinant jellyfish thioredoxin coding sequence was connected to the pET24a vector using the two restriction sites to construct the pET24a recombinant expression vector; after transformation into Escherichia coli BL21 (DE3), the expression was induced to obtain the recombinant protein as shown in SEQ ID NO: 3; affinity chromatography was used to separate and purify the protein through the 6 histidine tags (His-tag) at the N-terminus of the expression vector to obtain a relatively single target protein band; the purified protein solution was further dialyzed for 24 hours and then freeze-dried to obtain a recombinant jellyfish thioredoxin with transmembrane ability and significant antioxidant activity; the recombinant protein freeze-dried powder was accurately weighed according to the formula amount, and dissolved in deionized water to obtain the recombinant jellyfish thioredoxin stock solution;

[0014] S2: Preparation of oil phase: accurately weigh the corresponding oil phase matrix components according to the formula, mix and heat, stir and melt, and then keep warm and stir to obtain an oil phase solution;

[0015] S3: Preparation of aqueous phase: first dissolve sorbic acid in deionized water according to the formula, then add polysorbate 80 and glycerol to dissolve, heat, keep warm and stir to obtain an aqueous phase solution;

[0016] S4: Heat to keep the temperature of the oil phase solution and the water phase solution constant, slowly add the water phase solution to the oil phase solution, keep warm and move to a magnetic stirring water bath, and stir for 20 minutes;

[0017] S5: Keep warm for emulsification, cool down, and stir until a white and delicate milky paste is formed;

[0018] S6: After the temperature gradually cools down, add the recombinant aequorea thioredoxin stock solution into the cream according to the formula amount, and continue stirring until coagulation to obtain the recombinant aequorea thioredoxin cream.

[0019] Further, in step S2, the heating temperature is 80-85°C, the insulation temperature is 80-85°C, the insulation time is 15-25min, and the stirring rate is 300-400r / min;

[0020] Further, in step S3, the heating temperature is 80-85°C, the insulation temperature is 80-85°C, the insulation time is 15-25min, and the stirring rate is 300-400r / min;

[0021] Further, in step S4, the insulation temperature is 80-85° C., and the stirring rate is 300-400 r / min;

[0022] Furthermore, in step S6, the temperature after cooling is 25-30°C.

[0023] The third aspect of the present invention provides a use of the recombinant jellyfish thioredoxin cream as described above in the preparation of a product for protecting skin from ultraviolet radiation damage.

[0024] Furthermore, the skin ultraviolet radiation damage protection product refers to a product that protects human skin from ultraviolet radiation damage in various ways.

[0025] Furthermore, when used for protecting the skin from ultraviolet radiation damage, the effective amount of the recombinant aequorea thioredoxin in the recombinant aequorea thioredoxin cream is 0.5% to 2% of the mass of the cream.

[0026] Furthermore, the recombinant jellyfish thioredoxin cream is used in the preparation of a product that relieves symptoms such as dry skin, desquamation and wrinkles caused by ultraviolet radiation damage, significantly improves skin hyperplasia, reduces the malondialdehyde (MDA) content in skin tissue, and increases the activity of antioxidant enzymes.

[0027] The present invention has the advantages that:

[0028] 1. The total amount of jellyfish is huge and the resources are very rich. The present invention proposes and prepares an external ointment of jellyfish-derived antioxidants and applies it to the protection of the skin against ultraviolet radiation. The recombinant jellyfish thioredoxin involved in the present invention has efficient transmembrane ability and significant antioxidant activity. The recombinant jellyfish thioredoxin cream is prepared by mixing recombinant jellyfish thioredoxin with efficient transmembrane ability and significant antioxidant activity as the main component with a cream base. The preparation method is simple and the price is low. It can significantly reduce the adverse symptoms of dry skin, desquamation and wrinkles caused by ultraviolet radiation, improve epidermal hyperplasia and skin oxidative stress levels, and can significantly improve the skin's antioxidant capacity, thereby effectively preventing skin damage caused by ultraviolet radiation.

[0029] 2. The present invention is the first to use jellyfish-derived antioxidants for external use to protect the skin from ultraviolet radiation damage, providing a new approach for the development of safer and more efficient natural drugs to prevent ultraviolet radiation damage, providing a new source of drugs for preventing ultraviolet radiation damage to the skin, and also expanding new application areas for the development of jellyfish biological resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 The full-length gene coding sequence of chemically synthesized recombinant jellyfish thioredoxin (PTD-CcTrx1). The underlined part is the NdeⅠ and XhoⅠ nuclease sequences, the shaded part is the TAT-PTD gene coding sequence, and the middle part is the PTD-CcTrx1 gene coding sequence. The full length of the sequence is 357bp.

[0031] Figure 2This is the preparation process of PTD-CcTrx1 cream and its appearance observation.

[0032] Figure 3 Identification of the PTD-CcTrx1 cream type.

[0033] Figure 4 This is the experimental flow chart of Example 1.

[0034] Figure 5 This is the effect of PTD-CcTrx1 cream on the skin surface state induced by ultraviolet irradiation.

[0035] Figure 6 Effect of PTD-CcTrx1 cream on skin damage score caused by ultraviolet irradiation.

[0036] Figure 7 PTD-CcTrx1 cream improves skin tissue pathological changes caused by ultraviolet radiation.

[0037] Figure 8 PTD-CcTrx1 cream improves mouse skin hyperplasia caused by ultraviolet irradiation, where Figure A is a quantitative analysis of mouse epidermal thickness; Figure B is a quantitative analysis of mouse dermal thickness.

[0038] Fig. 9 Figure A shows that PTD-CcTrx1 cream reduces the increase of skin MDA caused by ultraviolet irradiation; Figure B shows that PTD-CcTrx1 cream resists the decrease of skin SOD activity caused by ultraviolet irradiation. DETAILED DESCRIPTION

[0039] The specific implementation methods provided by the present invention are described in detail below in conjunction with the examples. The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.

[0040] Example 1: Preparation of recombinant jellyfish thioredoxin stock solution

[0041] The preparation method of the recombinant jellyfish thioredoxin stock solution is as follows:

[0042] S1: Construction of recombinant plasmid. The transmembrane peptide (TAT-PTD) gene coding sequence (SEQ ID NO: 1) was fused with the thioredoxin (CcTrx1) coding sequence (SEQ ID NO: 2) of the jellyfish by chemical synthesis. The amino acid sequence of the recombinant jellyfish thioredoxin (PTD-CcTrx1) is shown in SEQ ID NO: 3, and the nucleotide coding sequence is shown in SEQ ID NO: 4. Specific restriction sites NdeⅠ and XhoⅠ (NdeI: 5'-CATATG-3', XhoI: 5'-CTCGAG-3', Figure 1). The DNA synthesis product and pET24a plasmid were digested by endonuclease respectively; the digested products were connected by T4 DNA ligase and transformed into Escherichia coli BL21 (DE3), and spread on a culture dish containing kanamycin (100 μg / mL) and cultured for 15 hours. After the successful recombination was confirmed by bacterial liquid PCR, enzyme digestion verification and other methods, the recombinant plasmid was verified by bidirectional sequencing using T7 Terminator as sequencing primer.

[0043] S2: Induced expression of recombinant protein. Add the correctly sequenced recombinant bacterial solution into liquid LB medium containing 100 μg / mL kanamycin and culture at 37°C and 250 r / min shaking until OD 600 The induction agent isopropyl-β-D-thiogalactoside (IPTG) was added for induction. The optimal expression conditions of the recombinant protein were 37°C, 0.2mM IPTG, and 150r / min for 18h. The cells were collected and the expression level of the recombinant protein was detected by SDS-PAGE.

[0044] S3: Purification of recombinant protein. The cells were collected by centrifugation, resuspended in binding buffer (NaH2PO4 20mM; NaCl 500mM; imidazole 30mM; pH = 7.4), and the supernatant was purified after ultrasonic lysis solution centrifugation (10 000×g, 10min); a high-purity recombinant jellyfish thioredoxin solution was obtained by purification using a nickel ion affinity chromatography column.

[0045] S4: Preparation of recombinant protein stock solution. The purified recombinant protein solution was further dialyzed for 24 hours and lyophilized to obtain recombinant jellyfish thioredoxin lyophilized powder; the recombinant protein lyophilized powder was accurately weighed according to the formula amount and dissolved in deionized water to obtain the recombinant protein stock solution. The results of protein immunoblotting experiments, antioxidant activity and transmembrane ability tests showed that the recombinant jellyfish thioredoxin (PTD-CcTrx1) had significant antioxidant activity and efficient transmembrane ability.

[0046] Example 2: Preparation of recombinant jellyfish thioredoxin cream

[0047] The cream base includes the following raw materials in percentage by mass: 6% of stearic acid, 1.6% of Span-80, 6% of octadecyl alcohol, 9% of liquid paraffin, 6% of white vaseline, 4.4% of polysorbate 80, 10% of glycerol, 0.2% of sorbic acid, and 56.8% of deionized water; the effective addition amount of recombinant jellyfish thioredoxin is 1.6% of the total mass of the cream.

[0048] The cream preparation method is as follows:

[0049] S1: Preparation of oil phase: According to the formula, 8.15 g of stearic acid, 2.20 g of Span-80, 8.15 g of octadecyl alcohol, 12.25 g of liquid paraffin, and 8.15 g of white vaseline were uniformly mixed, heated and stirred to dissolve, and the temperature was raised to 80°C.

[0050] S2: Preparation of aqueous phase: First, dissolve 0.34 g of sorbic acid in 77.35 mL of deionized water, then add 6 g of polysorbate 80 and 13.6 g of glycerol, and stir and heat to 80°C.

[0051] S3: Emulsification. When the water and oil phases are maintained at 80°C, the water phase is slowly added to the oil phase, and the mixture is moved to a magnetic stirring water bath pot, and the stirring rate is 400r / min, the temperature is maintained at 80°C, and the stirring is performed for 20 minutes; when the temperature is cooled to 25°C, an appropriate amount of the emulsion is taken, and the recombinant hair-shaped jellyfish thioredoxin stock solution is added to make its mass percentage concentration 1.6%, and the stirring is continued until coagulation, and the recombinant hair-shaped jellyfish thioredoxin cream ( Figure 2 ).

[0052] Example 3: Identification of the type of recombinant jellyfish thioredoxin cream

[0053] The type of recombinant jellyfish thioredoxin cream was identified by staining. A water-soluble dye, methylene blue, was dropped into the cream and observed under a microscope. If the external phase was blue, it was an oil-in-water type (O / W), otherwise it was a water-in-oil type (W / O). Figure 3 As shown, the external phase is blue and is an oil-in-water (O / W) cream type.

[0054] Example 4: Cream improves adverse skin symptoms caused by ultraviolet radiation

[0055] A model of ultraviolet radiation-induced damage to the back skin of mice was established to evaluate the effect of recombinant jellyfish thioredoxin cream on ultraviolet radiation-induced damage to the back skin of mice. Figure 4 shown.

[0056] 1. Experimental subjects

[0057] ICR mice (male) were fed in a standard animal room for one week before the experiment. The feeding environment was 23±2°C, 55±10%, 12h light / dark alternation, and food and water were provided in a fixed amount.

[0058] 2. Experimental Grouping

[0059] After adaptive feeding, the mice were randomly divided into 4 groups, with 6 mice in each group, namely control group (Control), ultraviolet irradiation group (PBS+UVB), cream matrix group (Cream+UVB) and PTD-CcTrx1 cream group (Cream+PTD-CcTrx1+UVB).

[0060] 3. Experimental methods

[0061] (1) Use a hand-held hair clipper and depilatory cream to clean the hair on the back of each group of ICR mice, exposing an area of ​​skin of approximately 3 cm × 3 cm;

[0062] (2) 30 min before irradiation, 0.1 mL of PBS, cream base, and PTD-CcTrx1 cream were applied to the exposed skin on the backs of mice in the UV irradiation group, cream base group, and PTD-CcTrx1 cream group, respectively;

[0063] (3) The exposed skin on the back of each group of mice was exposed to a UVB lamp (model: TL 20W / 01RS SLV / 25, PHILIPS), about 20 cm away from the mouse skin. The lamp was preheated for 5 min before UV irradiation, and the UV radiation intensity of the lamp was measured using an ultraviolet irradiance meter (model: LS125-UVB-X0 Probe, Shenzhen Linshang Technology Co., Ltd.). The irradiation dose was 20 mJ / cm 2 ;

[0064] (4) According to the above steps (2) and (3), apply and irradiate once a day for 10 consecutive days. Observe and record the damage of the exposed skin of each group of mice after ultraviolet irradiation. The scoring criteria for skin damage are shown in Table 1.

[0065] Table 1 Scoring criteria for skin damage caused by ultraviolet radiation

[0066]

[0067] 4. Experimental results

[0068] like Figure 5 As shown, the skin of the mice in the control group (Control) was delicate, without rough or shallow wrinkles, and was a healthy pink color; after 10 days of continuous irradiation, the back skin of the mice in the ultraviolet irradiation group (PBS+UVB) was dry, the skin was scaly, loose, and had obvious deep wrinkles; the cream matrix group (Cream+UVB) showed a skin appearance similar to that of the ultraviolet irradiation group; and after pre-application of the recombinant jellyfish thioredoxin cream, compared with the ultraviolet irradiation group and the cream matrix group, the irradiated parts of the mouse skin had only fine light stripes, and no deep wrinkles or skin sagging appeared.

[0069] like Figure 6 As shown, compared with the control group, the skin injury score of the UV irradiation group was significantly increased (P < 0.01), the scores of the cream matrix group and the UV irradiation group were similar (P > 0.05), and the skin injury score of the mice in the PTD-CcTrx1 cream group was significantly reduced compared with the UV irradiation group (P < 0.01).

[0070] Example 5: Cream improves skin hyperplasia in mice caused by UV irradiation damage

[0071] 1. Experimental methods

[0072] After the administration, the mice were sacrificed by dislocation of the neck, and a piece of skin with an area of ​​about 1.0 cm × 0.5 cm was cut with surgical scissors, cleaned with 0.9% saline to remove blood stains on the surface, and then immersed in 4% paraformaldehyde solution for histopathological observation. After H&E and Masson staining of the back skin tissue of the mice, the pathological changes of the skin were observed and the protective effect of PTD-CcTrx1 cream on ultraviolet radiation damage to the mouse skin was evaluated. CaseView2.4 image analysis software was used to analyze the thickness of the epidermis and dermis of the mouse skin.

[0073] 2. Experimental results

[0074] The results showed that UV radiation can cause skin hyperplasia, manifested by increased thickness of the epidermis and dermis. Figure 7 and Figure 8 As shown in the data, after UV irradiation, the thickness of the epidermis and dermis of the mice in the UV irradiation group was significantly increased compared with that in the control group (P < 0.01); there was no statistical difference in the thickness of the epidermis and dermis between the cream matrix group and the UV irradiation group (P > 0.05), while the thickness of the epidermis and dermis in the PTD-CcTrx1 cream group was significantly reduced compared with that in the UV irradiation group (P < 0.01), indicating that pre-application of recombinant jellyfish thioredoxin cream can significantly improve skin hyperplasia in mice induced by UV radiation.

[0075] Example 6: Cream improves oxidative stress level in skin tissue of UV-irradiated mice

[0076] 1. Experimental methods

[0077] (1) Mouse skin tissue collection, preservation and homogenization

[0078] After the administration, the mice were sacrificed by dislocating the neck, and the exposed skin tissue of about 2 cm × 2 cm was quickly removed with surgical scissors. The subcutaneous connective tissue and adipose tissue were carefully removed and placed in a homogenization tube, which was quickly placed in liquid nitrogen for rapid freezing, and then the samples were stored in a -80°C refrigerator for later use. During the experiment, the tissue was taken out for rewarming, the water was dried, the tissue weight was weighed, the tissue was cut into pieces with surgical scissors, and then the corresponding volume of buffer / lysis solution was added according to the instructions of the detection kit, and the tissue was homogenized for 15 minutes using a hybrid grinder at low temperature, and centrifuged at 12,000 r / min in a low-temperature centrifuge for 20 minutes. The supernatant was taken and stored in aliquots at -80°C for testing.

[0079] (2) Determination of protein concentration

[0080] The protein concentration of mouse skin tissue homogenate was determined using a BCA assay kit.

[0081] (3) Determination of MDA content and SOD activity in skin tissue

[0082] The MDA content and SOD activity were determined according to the instructions of the MDA detection kit (M496) and SOD detection kit (S311) purchased from DOJINDO.

[0083] 2. Experimental results

[0084] like Fig. 9 As shown, compared with the control group, the MDA content in the skin tissue of the UV irradiation group was significantly increased (P < 0.01), while the SOD activity was significantly decreased (P < 0.05), indicating that the skin was in a state of oxidative stress after UV irradiation; there were no statistical differences in the MDA content and SOD activity in the skin tissue of the cream matrix group and the UV irradiation group (P > 0.05); compared with the UV irradiation group, the MDA content in the skin tissue of the mice in the PTD-CcTrx1 cream group was significantly decreased (P < 0.05), and the SOD activity was significantly increased (P < 0.05), indicating that the recombinant jellyfish thioredoxin cream can significantly improve the oxidative stress level of UV-irradiated skin tissue after pre-application.

[0085] In summary, the recombinant jellyfish thioredoxin cream of the present invention can significantly alleviate the adverse skin symptoms caused by ultraviolet irradiation, and significantly improve skin hyperplasia and oxidative stress levels of skin tissue. The recombinant jellyfish thioredoxin cream of the present invention is easy to prepare, has great potential for preventing and treating ultraviolet damage to the skin, and has practical application value.

[0086] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A recombinant jellyfish thioredoxin cream, characterized in that: The cream is prepared by mixing recombinant aequorea thioredoxin as a main active ingredient with a cream base; the amino acid sequence of the recombinant aequorea thioredoxin is shown in SEQ ID NO: 3; The amount of recombinant jellyfish thioredoxin added to the cream is 0.5% to 2% relative to the mass of the cream base; the oil phase material of the cream base is composed of a mixture of five substances, namely stearic acid, Span-80, octadecyl alcohol, liquid paraffin, and white vaseline, and the water phase material is composed of a mixture of four substances, namely polysorbate 80, glycerol, sorbic acid, and deionized water.

2. The cream according to claim 1, characterized in that The mass percentage of each raw material in the cream base is 6%-8% of stearic acid, 1.6%-2% of Span-80, 6%-8% of octadecyl alcohol, 9%-10% of liquid paraffin, 6%-8% of white vaseline, 4%-5% of polysorbate 80, 10%-12% of glycerol, 0.2%-0.4% of sorbic acid, and the balance is deionized water.

3. A method for preparing the recombinant jellyfish thioredoxin cream according to claim 1 or 2, characterized in that: The following steps are involved: S1: Preparation of recombinant jellyfish thioredoxin stock solution: obtain the recombinant jellyfish thioredoxin gene coding sequence as shown in SEQ ID NO: 4 by chemical synthesis, with specific restriction sites NdeI: 5'-CATATG-3' and XhoI: 5'-CTCGAG-3' at both ends of the sequence, and construct pET24a recombinant expression vector; transform into Escherichia coli BL21 (DE3) and induce expression to obtain the recombinant protein as shown in SEQ ID NO: 3; separate, purify and freeze-dry by affinity chromatography to obtain recombinant jellyfish thioredoxin; weigh the recombinant protein freeze-dried powder according to the formula, and dissolve it in deionized water to obtain the recombinant jellyfish thioredoxin stock solution; S2: Preparation of oil phase: weigh the corresponding oil phase matrix components according to the formula of claim 2, mix and heat, stir and melt, and then keep warm and stir to obtain an oil phase solution; S3: Preparation of aqueous phase: first dissolve sorbic acid in deionized water according to the formula of claim 2, then add polysorbate 80 and glycerol to dissolve, heat, keep warm and stir to obtain an aqueous phase solution; S4: heating to keep the temperature of the oil phase solution and the water phase solution constant, slowly adding the water phase solution to the oil phase solution, keeping the temperature in a magnetic stirring water bath, and stirring for 20 minutes; S5: Keep warm for emulsification, cool down, and stir until a white and delicate milky paste is formed; S6: After the temperature gradually cools down, add the recombinant aequorea thioredoxin stock solution into the cream according to the formula amount, and continue stirring until coagulation to obtain the recombinant aequorea thioredoxin cream.

4. The preparation method according to claim 3, characterized in that: In steps S2 and S3, the heating temperature is 80-85° C., the insulation temperature is 80-85° C., the insulation time is 15-25 min, and the stirring rate is 300-400 r / min.

5. The preparation method according to claim 3, characterized in that: In step S4, the insulation temperature is 80-85° C., and the stirring rate is 300-400 r / min.

6. The preparation method according to claim 3, characterized in that: In step S6, the temperature after cooling is 25-30°C.

7. Use of the recombinant jellyfish thioredoxin cream according to claim 1 or 2 in the preparation of a product for protecting skin from ultraviolet radiation damage.

8. The use according to claim 7, characterized in that: The skin ultraviolet radiation damage protection product refers to a product that protects human skin from ultraviolet radiation damage.

9. The use according to claim 7, characterized in that: The recombinant jellyfish thioredoxin cream is used in the preparation of a product for alleviating symptoms such as dry skin, desquamation and wrinkles caused by ultraviolet radiation damage, significantly improving skin hyperplasia, reducing the malondialdehyde content in skin tissue, and increasing the activity of antioxidant enzymes.

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