Oral amino acid composition for relieving ulcerative colitis as well as preparation method and application thereof

Abstract

The invention discloses an oral amino acid composition for relieving ulcerative colitis as well as a preparation method and application thereof, and belongs to the technical field of oral amino acid compositions.The amino acid composition is prepared from glycine, alanine, proline, valine, leucine, phenylalanine and isoleucine and mainly aims at balanced nutrition supplement; the traditional Chinese medicine composition has a good curative effect on ulcerative colitis, the total effective rate reaches 70% or above, and the traditional Chinese medicine composition is low in raw material cost, easy and convenient to prepare and convenient to use, and has remarkable curative effects on relieving colitis, relieving hematochezia attack, mixing intestinal barriers, greatly reducing the deterioration probability of enteritis and the like through clinical application and pharmacological research for many years. Clinical symptoms such as stomachache, colon swelling and intestinal metabolism disorder caused by colitis, weight loss caused by malnutrition and the like can be improved, and the life quality of a patient is improved.

Description

Technical Field

[0001] The invention relates to an oral amino acid composition, in particular to an oral amino acid composition for relieving ulcerative colitis and a preparation method and application thereof, belonging to the technical field of oral amino acid compositions. Background Art

[0002] Ulcerative colitis is a chronic inflammatory disease that mainly affects the colon and rectum, usually extending proximally to the entire colon in a continuous manner. It is collectively referred to as inflammatory bowel disease together with Crohn's disease. The disease begins in the rectum, and the clinical manifestations are persistent or recurrent diarrhea, mucus, pus and blood in the stool accompanied by abdominal pain, weight loss and varying degrees of systemic symptoms. It has been confirmed that the pathogenic factors of ulcerative colitis are mainly related to the imbalance of intestinal microbial metabolism induced by the destruction of the intestinal mucosal barrier, the infiltration of inflammatory cells and the increase of oxidative stress levels. Its etiology and pathogenesis are complex. It is currently believed that genetic factors, environmental factors, host immune system disorders and intestinal microbial flora disorders are involved in the onset of ulcerative colitis. In recent years, the incidence of ulcerative colitis has shown an upward trend worldwide, and as a chronic disease, ulcerative colitis has a high recurrence rate, with a 10-year cumulative recurrence rate of patients as high as 70-80%. With the continuous progression of ulcerative colitis lesions and the prolonged course of the disease, the risk of poor prognosis for patients is also increasing. Therefore, it is urgent to find an effective and safe strategy to meet the huge challenge of UC treatment. Summary of the invention

[0003] The main purpose of the present invention is to provide an oral amino acid composition for relieving ulcerative colitis and a preparation method and application thereof.

[0004] The purpose of the present invention can be achieved by adopting the following technical solutions:

[0005] An oral amino acid composition for relieving ulcerative colitis, wherein the composition ratio of the oral amino acid composition is as follows:

[0006] 60-90 parts of glycine, 22-40 parts of alanine, 20-40 parts of proline, 8-30 parts of valine, 8-30 parts of leucine, 5-20 parts of phenylalanine, and 5-20 parts of isoleucine.

[0007] A method for preparing an oral amino acid composition for relieving ulcerative colitis, comprising mixing 60-90 parts of glycine, 22-40 parts of alanine, 20-40 parts of proline, 8-30 parts of valine, 8-30 parts of leucine, 5-20 parts of phenylalanine, and 5-20 parts of isoleucine;

[0008] After the combination is combined, the mixture is mixed in a mixing device.

[0009] Preferably, the mixing process adopts a mixing device, wherein the mixing device includes a base and a bracket fixedly mounted on one end of the top of the base, a quadrangular prism is vertically rotatably mounted on the upper bottom of the bracket, a sleeve is slidably mounted on the bottom of the quadrangular prism along the length direction, a rectangular groove cooperating with the quadrangular prism is provided inside the sleeve, a mixing rod head is installed on the outer side of the bottom end of the sleeve, a liquid injection hole is provided at the middle position of the bottom end of the sleeve, a rotating lifting mechanism for controlling the vertical lifting and horizontal rotation of the sleeve is installed on the bracket, an amino acid quantitative filling mechanism is provided at one end of the top of the base, an amino acid placement cup fixing mechanism is provided on the outer side of the bottom end of the sleeve, and an amino acid placement cup conveying mechanism is provided at the top of the base below the sleeve.

[0010] Preferably, the rotary lifting mechanism includes a driving motor, a screw, an inner screw tube, a first bearing, a connecting rod, a main gear and a sub-gear. The driving motor is mounted on a bracket, a screw is mounted on the output end of the driving motor, an inner screw tube is threadedly mounted on the screw, the first bearing is mounted on a sleeve, and the inner ring of the first bearing is fixedly connected to the sleeve, a connecting rod is fixed between the outer ring of the first bearing and the inner screw tube, a main gear is mounted on the top of the screw, and a sub-gear meshing with the main gear is fixed on the top of the quadrangular prism.

[0011] Preferably, the amino acid placement cup conveying mechanism includes a rotating motor, a turntable, a guide plate and a storage plate. The rotating motor is installed on the inner top of the base. The output end of the rotating motor is installed with a turntable. The top surface of the turntable is flush with the top of the base. A guide plate is obliquely provided at one end of the top of the base away from the bracket, and the guide plate extends to the top of the turntable. A storage plate is fixed at a position on the side of the base close to the guide plate.

[0012] Preferably, the amino acid quantitative filling mechanism includes a conducting hole, a rotary joint, a first piston, a liquid storage tank, a cylinder body, a second piston, a vertical rod, a horizontal rod, a first spring, a liquid inlet pipe and a liquid discharge pipe, the conducting hole is vertically opened inside the quadrangular prism, and the conducting hole passes through the quadrangular prism, the bottom end of the quadrangular prism is fixed with a first piston, and the first piston is attached to the inner wall of the rectangular groove, the top of the liquid injection hole is connected to the inside of the rectangular groove, the top of the quadrangular prism is installed with a rotary joint, the rotary joint extends to the top of the bracket, and the rotary joint is connected to the conducting hole. The interior of the cylinder is connected, the liquid storage tank is fixed on the top of the base, the cylinder body is vertically arranged on the side of the liquid storage tank, a second piston is slidably installed inside the cylinder body, a vertical rod is fixed on the top of the second piston, a cross rod is slidably installed on the top of the vertical rod along the length direction of the vertical rod, a first spring is installed between the bottom of the cross rod and the top of the cylinder body, the vertical rod passes through the inside of the first spring, a liquid inlet pipe is provided between the bottom of the cylinder body and the inside of the liquid storage tank, a liquid discharge pipe is installed between the bottom of the cylinder body and the rotary joint, and a one-way valve is provided on both the liquid inlet pipe and the liquid discharge pipe.

[0013] Preferably, a sliding sleeve is slidably mounted on the vertical rod, the horizontal rod is fixed on the surface of the sliding sleeve, and a first positioning bolt is threadedly mounted on the outer side of the sliding sleeve;

[0014] The amino acid placement cup fixing mechanism includes a mounting plate, a second bearing, a second spring, a pressing plate, a sliding rod, a positioning block, a perforation, a U-shaped frame, an arc-shaped clamping plate and a third spring. The mounting plate is horizontally arranged on the outside of the sleeve. A second bearing is installed on the mounting plate, and the inner ring of the second bearing is fixed to the outside of the sleeve. A second spring is installed at the bottom of the mounting plate, and a pressing plate is horizontally installed at the bottom of the second spring. Both ends of the top of the pressing plate are vertically fixed with sliding rods. The sliding rods penetrate through the mounting plate and are slidably connected with the mounting plate. The top ends of the sliding rods are all fixed with positioning blocks. A perforation for the sleeve to pass through is formed on the pressing plate. Both ends of the bottom of the pressing plate are hingedly installed with U-shaped frames, and the corners at the top ends of the U-shaped frames are hinged to the pressing plate. Third springs are installed between the top ends of the U-shaped frames and the pressing plate. Arc-shaped clamping plates are installed at the bottom ends of the U-shaped frames.

[0015] Preferably, a sponge pad is fixed to the bottom of the pressing plate. Notch grooves are formed on both sides of the sponge pad. The top ends of the U-shaped frames are located inside the notch grooves.

[0016] A guide rod is vertically installed at the upper bottom of the bracket. The guide rod is slidably connected with the mounting plate. A contact switch is installed at the bottom end of the guide rod, and the contact switch is electrically connected to the rotating motor through a wire.

[0017] Preferably, the U-shaped frame includes an upper L-shaped plate, a lower L-shaped plate, a chute, a second positioning bolt, a sliding plate and a fourth positioning bolt. The upper L-shaped plate and the lower L-shaped plate are symmetrically arranged. The lower L-shaped plate is slidably arranged at the bottom of the upper L-shaped plate. A chute matching the upper L-shaped plate is formed at the top of the lower L-shaped plate. A second positioning bolt is installed at the side of the top end of the lower L-shaped plate. A sliding plate is slidably installed at the bottom end of the lower L-shaped plate. The sliding plate is fixed to the outside of the arc-shaped clamping plate. A fourth positioning bolt is installed at the inner top of the lower L-shaped plate.

[0018] An application of an oral amino acid composition for relieving ulcerative colitis. C57BL / 6J male mice were purchased from Guangzhou Regene Biotechnology Co., Ltd. All mice were fed with sterile water and autoclaved food in individually ventilated cages. The animals were raised under specific pathogen-free conditions, with a temperature of 22±2°C, a humidity of 50±5%, and a light-dark cycle of 12 h / time. The experiment was approved by the Animal Ethics Committee of the School of Chinese Medicine, Guangzhou University of Chinese Medicine.

[0019] After 1 week of adaptive feeding, the mice were randomly divided into 3 groups: a normal control group Cont, a model control 3% DSS group, and a 3% DSS + HAAs group.

[0020] The control group drank sterile water, and the other groups drank 3% DSS freely. At the same time, the animals in the Cont group and Mod group were given physiological saline intervention, and the animals in the HAAs group were given corresponding concentration gavage intervention. Starting from the day before modeling, the mice were scored for the disease activity index (DAI) to evaluate the severity of colitis, including the weight change rate of the mice, the average scores of loose stools and bloody stools. On the 8th day, the animals were anesthetized with pentobarbital and the colon and whole blood of the mice were collected. Some of them were stored at -80℃, the spleen was weighed, and the spleen coefficient was calculated. The other part of the tissue was fixed with 4% paraformaldehyde for subsequent experiments.

[0021] Beneficial technical effects of the present invention:

[0022] The present invention provides an oral amino acid composition for relieving ulcerative colitis, and a preparation method and application thereof. A hydrophobic amino acid (HAAs) composition is obtained through preliminary research, which is composed of glycine, alanine, proline, valine, leucine, phenylalanine, and isoleucine in a certain proportion. In order to explore whether it has anti-inflammatory activity, oral HAAs intervention is performed on mice with ulcerative colitis. It is found that after intervention, the weight loss, increased DAI score, shortened colon and other symptoms of mice caused by ulcerative colitis can be significantly alleviated, and colon tissue damage can be reduced, thereby effectively alleviating UC, or it can be further developed into a functional food for alleviating UC.

[0023] By arranging a rotating lifting mechanism composed of a driving motor, a screw, an inner screw tube, a first bearing, a connecting rod, a main gear and a sub-gear at the bottom of the bracket, a linkage control method can be adopted during the mixing process to simultaneously control the rotation and lifting of the mixing blade, making the mixing more convenient and more practical.

[0024] By installing a mixing blade on the outside of the bottom end of the sleeve, and opening a liquid injection hole at the middle position of the bottom of the sleeve, and then using it in conjunction with an amino acid quantitative filling mechanism composed of a guide hole, a rotary joint, a first piston, a liquid storage tank, a cylinder body, a second piston, a vertical rod, a horizontal rod, a first spring, a liquid inlet pipe, and a liquid discharge pipe, the amino acids can be automatically and quantitatively added during the amino acid mixing process, and the mixing is more uniform. The amino acids are discharged by extrusion, and the addition is faster, thereby improving the mixing rate.

[0025] The cup fixing mechanism is composed of the outer mounting plate of the sleeve, the second bearing, the second spring, the pressing plate, the sliding rod, the positioning block, the perforation, the yoke, the arc clamping plate and the third spring. As the sleeve moves downward, the side of the cup can be automatically squeezed and fixed, and the top of the cup can be covered, which effectively avoids splashing of amino acids and floating of dust during mixing, and is more environmentally friendly.

[0026] The L-shaped frame composed of an upper L-shaped plate, a lower L-shaped plate, a slide groove, a second positioning bolt, a slide plate and a fourth positioning bolt can adjust the position of the arc-shaped clamping plate according to the size of the cup during use, so as to adapt to different types of cups and be more convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a front view of a preferred embodiment of an oral amino acid composition for relieving ulcerative colitis and a preparation method and application thereof of the present invention;

[0028] Figure 2 It is a front cross-sectional view of a preferred embodiment of an oral amino acid composition for relieving ulcerative colitis and a preparation method and application thereof of the present invention;

[0029] Figure 3 It is a cross-sectional view of the inner part of a sleeve of a preferred embodiment of an oral amino acid composition for relieving ulcerative colitis and a preparation method and application thereof of the present invention;

[0030] Figure 4 A diagram of a quantitative nutrient solution filling mechanism of a preferred embodiment of an oral amino acid composition for relieving ulcerative colitis and a preparation method and application thereof of the present invention;

[0031] Figure 5 This is a preferred embodiment of an oral amino acid composition for relieving ulcerative colitis and its preparation method and application. Figure 1 Enlarged view of point A in the middle;

[0032] Figure 6 A diagram of a flowerpot fixing mechanism of a preferred embodiment of an oral amino acid composition for relieving ulcerative colitis and a preparation method and application thereof of the present invention;

[0033] Figure 7 It is a structural diagram of a preferred embodiment of an oral amino acid composition for relieving ulcerative colitis and a preparation method and application thereof of the present invention;

[0034] Figure 8 This is a gear set structure diagram of a preferred embodiment of an oral amino acid composition for relieving ulcerative colitis and a preparation method and application thereof of the present invention;

[0035] Fig. 9Fig. 1. HAAs improve the symptoms of ulcerative colitis mice induced by sodium dextran sulfate, (A) weight change rate, (B) loose stool score, (C) blood in stool score, (D) weight score, (E) DAI score, (F) representative picture of colon, (G) colon length statistics, (H) spleen index, (n=6 per group), (compared with Cont group *P<0.05.**P<0.01.***P<0.001, compared with Mod group #P<0.05.##P<0.01.###P<0.001.####P<0.0001)

[0036] In the figure: 1-,. DETAILED DESCRIPTION

[0037] In order to make the technical solution of the present invention more clear and specific to those skilled in the art, the present invention is further described in detail below in conjunction with embodiments and drawings, but the implementation manner of the present invention is not limited thereto.

[0038] like Figure 1-Figure 8 As shown, this embodiment provides an oral amino acid composition for relieving ulcerative colitis, and the composition ratio of the oral amino acid composition is as follows:

[0039] 60-90 parts of glycine, 22-40 parts of alanine, 20-40 parts of proline, 8-30 parts of valine, 8-30 parts of leucine, 5-20 parts of phenylalanine, and 5-20 parts of isoleucine.

[0040] A method for preparing an oral amino acid composition for relieving ulcerative colitis, comprising mixing 60-90 parts of glycine, 22-40 parts of alanine, 20-40 parts of proline, 8-30 parts of valine, 8-30 parts of leucine, 5-20 parts of phenylalanine, and 5-20 parts of isoleucine;

[0041] After the combination is combined, the mixture is mixed in a mixing device.

[0042] This mixing process uses a mixing device. The mixing device includes a base 1 and a bracket 2 fixedly installed at one end of the top of the base 1. A quadrangular prism 3 is vertically rotatably installed on the upper bottom of the bracket 2. A sleeve 4 is slidably provided along the length direction at the bottom of the quadrangular prism 3. A rectangular groove 5 that mates with the quadrangular prism 3 is provided inside the sleeve 4. A mixing cutter head 6 is installed on the outer side of the bottom end of the sleeve 4. A liquid injection hole 7 is provided at the middle position of the bottom end of the sleeve 4. A rotary lifting mechanism for controlling the vertical lifting and horizontal rotation of the sleeve 4 is installed on the bracket 2. An amino acid quantitative filling mechanism 8 is provided at one end of the top of the base 1. An amino acid placement cup fixing mechanism 9 is provided on the outer side of the bottom end of the sleeve 4. An amino acid placement cup conveying mechanism is provided at the position below the sleeve 4 on the top of the base 1.

[0043] By providing a rotary lifting mechanism composed of a drive motor 10, a screw 11, an internal screw tube 12, a first bearing 13, a connecting rod 14, a main gear 15, and a sub-gear 16 at the bottom of the bracket 2, during the mixing process, a linkage control method can be adopted to simultaneously control the rotation and lifting of the mixing cutter head 6, making the mixing more convenient and more practical. By installing the mixing cutter head 6 on the outer side of the bottom end of the sleeve 4 and providing the liquid injection hole 7 at the middle position of the bottom of the sleeve 4, and then cooperating with the amino acid quantitative filling mechanism composed of a through hole 21, a rotary joint 22, a first piston 23, a liquid storage tank 24, a cylinder block 25, a second piston 26, a vertical rod 27, a cross rod 28, a first spring 29, a liquid inlet pipe 30, and a liquid discharge pipe 31 for use, automatic quantitative addition of amino acids can be carried out during the amino acid mixing process, making the mixing more uniform, and the amino acids are discharged by an extrusion method, with the addition being faster, thus improving the mixing rate. Through the amino acid placement cup fixing mechanism composed of an external mounting plate 34, a second bearing 35, a second spring 36, a pressing plate 37, a sliding rod 38, a positioning block 39, a perforation 40, a U-shaped frame 41, an arc-shaped clamping plate 42, and a third spring 43 on the outside of the sleeve 4, as the sleeve 4 moves downward, it can automatically squeeze and fix the side of the amino acid placement cup and cover the top of the amino acid placement cup, effectively avoiding the splashing of amino acids during mixing and the floating of dust, making the use more environmentally friendly. The U-shaped frame 41 composed of an upper L-shaped plate 48, a lower L-shaped plate 49, a chute 50, a second positioning bolt 51, a sliding plate 52, and a fourth positioning bolt 53 can adjust the position of the arc-shaped clamping plate 42 according to the size of the amino acid placement cup during use, so as to adapt to different types of amino acid placement cups, making the use more convenient.

[0044] In this embodiment, the rotary lifting mechanism includes a driving motor 10, a screw 11, an inner screw tube 12, a first bearing 13, a connecting rod 14, a main gear 15 and a sub-gear 16. The driving motor 10 is installed on the bracket 2, and the output end of the driving motor 10 is installed with a screw 11, and the inner screw tube 12 is threadedly installed on the screw 11. The first bearing 13 is installed on the sleeve 4, and the inner ring of the first bearing 13 is fixedly connected to the sleeve 4. A connecting rod 14 is fixed between the outer ring of the first bearing 13 and the inner screw tube 12. The main gear 15 is installed at the top of the screw 11, and the sub-gear 16 meshing with the main gear 15 is fixed on the top of the quadrangular prism 3.

[0045] Local working principle: the drive motor 10 starts to drive the screw 11 to rotate, and the inner screw tube 12 moves along the length direction of the screw 11. The inner screw tube 12 cooperates with the connecting rod 14 and the first bearing 13 to control the up and down movement of the sleeve 4 during the movement. During the rotation of the screw 11, the main gear 15 is also driven to rotate. The main gear 15 is engaged with the sub-gear 16 on the quadrangular prism 3, and the quadrangular prism 3 also rotates. The quadrangular prism 3 drives the sleeve 4 to rotate, and the sleeve 4 rotates and rises in this process.

[0046] In this embodiment, the amino acid placement cup conveying mechanism includes a rotating motor 17, a turntable 18, a guide plate 19 and a storage plate 20. The rotating motor 17 is installed on the inner top of the base 1. The turntable 18 is installed on the output end of the rotating motor 17. The top surface of the turntable 18 is flush with the top of the base 1. A guide plate 19 is obliquely provided at one end of the top of the base 1 away from the bracket 2, and the guide plate 19 extends to the top of the turntable 18. A storage plate 20 is fixed to the side of the base 1 near the guide plate 19.

[0047] Local working principle: Before use, the amino acid placement cups are evenly placed on the top of the turntable 18, and the angle between adjacent turntables 18 is ninety degrees. During a single conveying process, the rotating motor 17 only needs to drive the turntable 18 to rotate ninety degrees to change the position of the amino acid placement cup, and the mixed amino acid placement cup is conveyed to the bottom of the sleeve 4. After the mixing is completed, as the turntable 18 rotates, the amino acid placement cup rotates toward the guide plate 19, slides to the outside of the turntable 18 under the blocking action of the guide plate 19, and is stored on the storage plate 20.

[0048] In this embodiment, the amino acid quantitative filling mechanism 8 includes a conducting hole 21, a rotary joint 22, a first piston 23, a liquid storage box 24, a cylinder body 25, a second piston 26, a vertical rod 27, a horizontal rod 28, a first spring 29, a liquid inlet pipe 30 and a liquid discharge pipe 31. The conducting hole 21 is vertically opened inside the quadrangular prism 3, and the conducting hole 21 passes through the quadrangular prism 3. The first piston 23 is fixed to the bottom end of the quadrangular prism 3, and the first piston 23 is attached to the inner wall of the rectangular groove 5. The top of the injection hole 7 is connected to the inside of the rectangular groove 5. The top of the quadrangular prism 3 is equipped with a rotary joint 22, which extends to the top of the bracket 2, and the rotary joint 22 is connected to the conducting hole 2. 1 is internally connected, a liquid storage tank 24 is fixed on the top of the base 1, a cylinder body 25 is vertically arranged on the side of the liquid storage tank 24, a second piston 26 is slidably installed inside the cylinder body 25, a vertical rod 27 is fixed on the top of the second piston 26, a cross bar 28 is slidably installed on the top of the vertical rod 27 along the length direction of the vertical rod 27, a first spring 29 is installed between the bottom of the cross bar 28 and the top of the cylinder body 25, the vertical rod 27 passes through the inside of the first spring 29, a liquid inlet pipe 30 is provided between the bottom of the cylinder body 25 and the inside of the liquid storage tank 24, a liquid discharge pipe 31 is installed between the bottom of the cylinder body 25 and the rotary joint 22, and a one-way valve is provided on both the liquid inlet pipe 30 and the liquid discharge pipe 31.

[0049] Partial working principle: When the first spring 29 is in the reset state, the second piston 26 is located at the inner top of the cylinder body 25. During the upward movement of the second piston 26, amino acids are extracted from the inside of the liquid storage tank 24 through the liquid inlet pipe 30 and stored in the inside of the cylinder body 25. During the mixing process, when the inner spiral tube 12 moves downward, it will exert pressure on the cross bar 28, driving the cross bar 28 to move downward. The cross bar 28 drives the vertical rod 27 and the second piston 26 to move downward at the same time, and the amino acids in the cylinder body 25 are discharged from the inside of the discharge pipe 31, injected into the inside of the rectangular groove 5 through the rotary joint 22 and the conducting hole 21, and then discharged into the inside of the cup through the injection hole 7, so that the amino acids are added at a uniform and quantitative speed.

[0050] In this embodiment, a sliding sleeve 32 is slidably mounted on the vertical rod 27 , the horizontal rod 28 is fixed on the surface of the sliding sleeve 32 , and a first positioning bolt 33 is threadedly mounted on the outer side of the sliding sleeve 32 .

[0051] Partial working principle: Due to the different sizes of the amino acid placement cups, the sliding sleeve 32 slides along the length direction of the vertical rod 27, and the position is fixed by the first positioning bolt 33. By adjusting the position of the cross bar 28 on the vertical rod 27, the amount of amino acid added can be controlled, which is more convenient to use.

[0052] In this embodiment, the amino acid placement cup fixing mechanism 9 includes a mounting plate 34, a second bearing 35, a second spring 36, a pressing plate 37, a sliding rod 38, a positioning block 39, a perforation 40, a C-shaped frame 41, an arc-shaped clamping plate 42, and a third spring 43. The mounting plate 34 is horizontally arranged outside the sleeve 4. The second bearing 35 is mounted on the mounting plate 34, and the inner ring of the second bearing 35 is fixed to the outside of the sleeve 4. The second spring 36 is mounted at the bottom of the mounting plate 34. The bottom of the second spring 36 is horizontally mounted with the pressing plate 37. Both ends of the top of the pressing plate 37 are vertically fixed with sliding rods 38. The sliding rods 38 penetrate through the mounting plate 34 and are slidably connected to the mounting plate 34. The top ends of the sliding rods 38 are both fixed with positioning blocks 39. A perforation 40 for the sleeve 4 to pass through is formed on the pressing plate 37. Both ends of the bottom of the pressing plate 37 are hingedly mounted with C-shaped frames 41, and the corners at the top ends of the C-shaped frames 41 are hinged to the pressing plate 37. Third springs 43 are mounted between the top ends of the C-shaped frames 41 and the pressing plate 37. Arc-shaped clamping plates 42 are mounted at the bottom ends of the C-shaped frames 41.

[0053] Local working principle: During the downward movement of the sleeve 4, it will drive the mounting plate 34 to move downward simultaneously. When the bottom end of the sleeve 4 is inserted into the inside of the amino acid placement cup, since the amino acid is lower than the horizontal plane of the top end of the amino acid placement cup, it will not contact the amino acid. Then, as the sleeve 4 continues to move downward, the pressing plate 37 covers the top of the amino acid placement cup. At the same time, the top end of the amino acid placement cup presses against the C-shaped frame 41, and the arc-shaped clamping plates 42 on the C-shaped frame 41 clamp and fix the side of the amino acid placement cup, and cover the top of the amino acid placement cup through the pressing plate 37. After the amino acid placement cup is positioned and covered, the sleeve 4 continues to move downward. At this time, the mixing cutter head 6 performs mixing operation on the amino acid.

[0054] In this embodiment, a sponge pad 44 is fixed to the bottom of the pressing plate 37. Notch grooves 45 are formed on both sides of the sponge pad 44. The top ends of the C-shaped frames 41 are located inside the notch grooves 45.

[0055] Local working principle: The use of the sponge pad 44 can ensure the covering effect of the bottom end of the pressing plate 37 on the top of the amino acid placement cup, and avoid the splashing of amino acids and the floating of dust.

[0056] In this embodiment, a guide rod 46 is vertically mounted on the upper bottom of the bracket 2. The guide rod 46 is slidably connected to the mounting plate 34. A contact switch 47 is mounted at the bottom end of the guide rod 46, and the contact switch 47 is electrically connected to the rotary motor 17 through a wire.

[0057] Local working principle: After the amino acids in a single amino acid placement cup are mixed, the mounting plate 34 is reset upward, and after reaching the topmost position, the contact switch 47 is squeezed, at which time the contact switch 47 controls the rotary motor 17 to rotate ninety degrees, pushing out the mixed amino acid placement cup and transferring the unmixed amino acid placement cup to the bottom of the sleeve 4.

[0058] In this embodiment, the L-shaped frame 41 includes an upper L-shaped plate 48, a lower L-shaped plate 49, a slide groove 50, a second positioning bolt 51, a slide plate 52 and a fourth positioning bolt 53. The upper L-shaped plate 48 and the lower L-shaped plate 49 are symmetrically arranged. The lower L-shaped plate 49 is slidably arranged at the bottom of the upper L-shaped plate 48. The top of the lower L-shaped plate 49 is provided with a slide groove 50 that cooperates with the upper L-shaped plate 48. The side edge of the top of the lower L-shaped plate 49 is provided with a second positioning bolt 51. The bottom end of the lower L-shaped plate 49 is slidably installed with a slide plate 52. The slide plate 52 is fixed to the outer side of the arc-shaped clamping plate 42. The fourth positioning bolt 53 is installed at the inner top of the lower L-shaped plate 49.

[0059] Local working principle: Since the sizes of amino acid placement cups are different, in order to adapt to different amino acid placement cups, during use, the upper L-shaped plate 48 and the lower L-shaped plate 48 are controlled to slide, and the overall length of the L-shaped frame 41 is changed to adapt to amino acid placement cups of different heights. In addition, by controlling the sliding of the slide plate 52, the position of the arc-shaped clamping plate 42 is changed to adapt to amino acid placement cups of different diameters.

[0060] An application of an oral amino acid composition for relieving ulcerative colitis. C57BL / 6J male mice were purchased from Guangzhou Regal Biotechnology Co., Ltd. All mice were fed with sterile water and autoclaved food in independent ventilated cages. The animals were kept under specific pathogen-free conditions with a temperature of 22±2°C, a humidity of 50±5%, and a light-dark cycle of 12h / time. The experiment was approved by the Animal Ethics Committee of the School of Traditional Chinese Medicine of Guangzhou University of Chinese Medicine.

[0061] After the mice were adapted to feeding for 1 week, they were randomly divided into 3 groups: normal control group Cont, model control 3% DSS group, and 3% DSS+HAAs group;

[0062] The control group drank sterile water, and the other groups drank 3% DSS freely. At the same time, the animals in the Cont group and Mod group were given physiological saline intervention, and the animals in the HAAs group were given corresponding concentration gavage intervention. Starting from the day before modeling, the mice were scored for the disease activity index (DAI) to evaluate the severity of colitis, including the weight change rate of the mice, the average scores of loose stools and bloody stools. On the 8th day, the animals were anesthetized with pentobarbital and the colon and whole blood of the mice were collected. Some of them were stored at -80℃, the spleen was weighed, and the spleen coefficient was calculated. The other part of the tissue was fixed with 4% paraformaldehyde for subsequent experiments.

[0063] Previous research results

[0064] In order to explore whether HAAs have a potential alleviating effect on dextran sulfate sodium-induced ulcerative colitis in mice, animal experiments were used to verify the effect. 7-8 week-old C57 mice were selected for adaptive feeding for 7 days, and then dextran sulfate sodium was used to establish an ulcerative colitis mouse model. At the same time, HAAs were gavaged for 7 days. During this period, the weight of the mice was recorded and the DAI score was calculated. Figure 1 As shown in Figure .A, the weight of mice was significantly reduced after modeling; in addition, the loose stool scores of mice in the Mod group after modeling ( Figure 1 .B) Stool blood score ( Figure 1 .C) and weight score ( Figure 1 .D) and DAI scores (Fig.1.E) were significantly higher than those of the Cont group mice; after HAAs gavage intervention, the weight and scores of the mice recovered to varying degrees. The colon length of the mice after dextran sulfate sodium modeling was significantly shorter than that of the Cont group (e.g. Figure 1 .F), there is a statistically significant difference (such as Figure 1 .G). It is worth noting that colon shortening was significantly improved after HAAs intervention. In addition, the spleen of mice was significantly enlarged after modeling ( Figure 1 .H), all recovered to varying degrees after HAAs intervention. Overall, the clinical symptoms of UC mice were alleviated to varying degrees after HAAs intervention. It may be further developed into a functional food that can relieve ulcerative colitis

[0065] The above description is only a further embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and concepts of the present invention within the scope disclosed by the present invention, which belong to the protection scope of the present invention.

Claims

1. An oral amino acid composition for relieving ulcerative colitis, characterized in that: The oral amino acid composition comprises the following components: 60-90 parts of glycine, 22-40 parts of alanine, 20-40 parts of proline, 8-30 parts of valine, 8-30 parts of leucine, 5-20 parts of phenylalanine, and 5-20 parts of isoleucine.

2. A method for preparing an oral amino acid composition for relieving ulcerative colitis, based on the oral amino acid composition for relieving ulcerative colitis according to claim 1, characterized in that: The composition comprises 60-90 parts of glycine, 22-40 parts of alanine, 20-40 parts of proline, 8-30 parts of valine, 8-30 parts of leucine, 5-20 parts of phenylalanine and 5-20 parts of isoleucine; After the combination is combined, the mixture is mixed in a mixing device.

3. The method for preparing an oral amino acid composition for relieving ulcerative colitis according to claim 2, characterized in that: The mixing process adopts a mixing device, wherein the mixing device comprises a base (1) and a bracket (2) fixedly mounted on one end of the top of the base (1); a quadrangular prism (3) is vertically rotatably mounted on the upper bottom of the bracket (2); a sleeve (4) is slidably mounted on the bottom of the quadrangular prism (3) along the length direction; a rectangular groove (5) matching the quadrangular prism (3) is provided inside the sleeve (4); a mixing rod head (6) is mounted on the outer side of the bottom end of the sleeve (4); a liquid injection hole (7) is provided at the middle position of the bottom end of the sleeve (4); a rotating lifting mechanism for controlling the vertical lifting and horizontal rotation of the sleeve (4) is mounted on the upper side of the bracket (2); an amino acid quantitative filling mechanism (8) is provided on one end of the top of the base (1); an amino acid placement cup fixing mechanism (9) is provided on the outer side of the bottom end of the sleeve (4); and an amino acid placement cup conveying mechanism is provided at the top of the base (1) below the sleeve (4).

4. The method for preparing an oral amino acid composition for relieving ulcerative colitis according to claim 3, characterized in that: The rotary lifting mechanism comprises a driving motor (10), a screw (11), an inner screw tube (12), a first bearing (13), a connecting rod (14), a main gear (15) and a sub gear (16). The driving motor (10) is mounted on a bracket (2). The output end of the driving motor (10) is mounted with a screw (11). The inner screw tube (12) is threadedly mounted on the screw (11). The first bearing (13) is mounted on a sleeve (4). The inner ring of the first bearing (13) is fixedly connected to the sleeve (4). The connecting rod (14) is fixed between the outer ring of the first bearing (13) and the inner screw tube (12). The main gear (15) is mounted on the top of the screw (11). The sub gear (16) meshing with the main gear (15) is fixed on the top of the quadrangular prism (3).

5. The method for preparing an oral amino acid composition for relieving ulcerative colitis according to claim 4, characterized in that: The amino acid placement cup conveying mechanism comprises a rotating motor (17), a rotating disk (18), a guide plate (19) and a storage plate (20), wherein the rotating motor (17) is mounted on the inner top of a base (1), and the output end of the rotating motor (17) is mounted with a rotating disk (18), the top surface of the rotating disk (18) is flush with the top of the base (1), the guide plate (19) is obliquely arranged at one end of the top of the base (1) away from the bracket (2), and the guide plate (19) extends to the top of the rotating disk (18), and the storage plate (20) is fixed at a position close to the guide plate (19) on the side of the base (1).

6. The method for preparing an oral amino acid composition for relieving ulcerative colitis according to claim 5, characterized in that: The amino acid quantitative filling mechanism (8) comprises a conducting hole (21), a rotating joint (22), a first piston (23), a liquid storage box (24), a cylinder body (25), a second piston (26), a vertical rod (27), a horizontal rod (28), a first spring (29), a liquid inlet pipe (30) and a liquid discharge pipe (31). The conducting hole (21) is vertically opened inside the quadrangular prism (3), and the conducting hole (21) passes through the quadrangular prism (3). The first piston (23) is fixed at the bottom end of the quadrangular prism (3), and the first piston (23) is attached to the inner wall of the rectangular groove (5). The top of the liquid injection hole (7) is in conduction with the inside of the rectangular groove (5). The top of the quadrangular prism (3) is equipped with a rotating joint (22), and the rotating joint (22) extends to the top of the bracket (2). The rotating joint (22) is connected to the conducting hole (2 1), the liquid storage tank (24) is fixed on the top of the base (1), the cylinder body (25) is vertically arranged on the side of the liquid storage tank (24), a second piston (26) is slidably installed inside the cylinder body (25), a vertical rod (27) is fixed on the top of the second piston (26), a cross rod (28) is slidably installed on the top of the vertical rod (27) along the length direction of the vertical rod (27), a first spring (29) is installed between the bottom of the cross rod (28) and the top of the cylinder body (25), the vertical rod (27) passes through the inside of the first spring (29), a liquid inlet pipe (30) is provided between the bottom of the cylinder body (25) and the inside of the liquid storage tank (24), a liquid discharge pipe (31) is installed between the bottom of the cylinder body (25) and the rotary joint (22), and a one-way valve is provided on both the liquid inlet pipe (30) and the liquid discharge pipe (31).

7. The method for preparing an oral amino acid composition for relieving ulcerative colitis according to claim 6, characterized in that: A sliding sleeve (32) is slidably mounted on the vertical rod (27), the horizontal rod (28) is fixed on the surface of the sliding sleeve (32), and a first positioning bolt (33) is threadedly mounted on the outer side of the sliding sleeve (32); The amino acid placement cup fixing mechanism (9) includes a mounting plate (34), a second bearing (35), a second spring (36), a pressing plate (37), a sliding rod (38), a positioning block (39), a perforation (40), a C-shaped frame (41), an arc-shaped clamping plate (42) and a third spring (43). The mounting plate (34) is horizontally arranged on the outside of the sleeve (4). The second bearing (35) is mounted on the mounting plate (34). The inner ring of the second bearing (35) is fixed to the outside of the sleeve (4). The second spring (36) is mounted on the bottom of the mounting plate (34). The bottom of the second spring (36) is horizontally mounted with the pressing plate (37). Both ends of the top of the pressing plate (37) are vertically fixed with sliding rods (38). The sliding rods (38) penetrate through the mounting plate (34) and are slidably connected with the mounting plate (34). The top ends of the sliding rods (38) are all fixed with positioning blocks (39). A perforation (40) for the sleeve (4) to pass through is formed on the pressing plate (37). Both ends of the bottom of the pressing plate (37) are hingedly mounted with C-shaped frames (41). And the corners at the top ends of the C-shaped frames (41) are hinged to the pressing plate (37). Third springs (43) are mounted between the top ends of the C-shaped frames (41) and the pressing plate (37). Arc-shaped clamping plates (42) are mounted at the bottom ends of the C-shaped frames (41).

8. The method for preparing an oral amino acid composition for relieving ulcerative colitis according to claim 7, characterized in that: A sponge pad (44) is fixed to the bottom of the pressing plate (37). Notch grooves (45) are formed on both sides of the sponge pad (44). The top ends of the C-shaped frames (41) are located inside the notch grooves (45). A guide rod (46) is vertically mounted on the upper bottom of the bracket (2). The guide rod (46) is slidably connected with the mounting plate (34). A contact switch (47) is mounted at the bottom end of the guide rod (46). And the contact switch (47) is electrically connected to the rotating motor (17) through a wire.

9. The method for preparing an oral amino acid composition for relieving ulcerative colitis according to claim 8, characterized in that: The C-shaped frame (41) includes an upper L-shaped plate (48), a lower L-shaped plate (49), a sliding groove (50), a second positioning bolt (51), a sliding plate (52) and a fourth positioning bolt (53). The upper L-shaped plate (48) and the lower L-shaped plate (49) are symmetrically arranged. The lower L-shaped plate (49) is slidably arranged at the bottom of the upper L-shaped plate (48). A sliding groove (50) matching the upper L-shaped plate (48) is formed on the top of the lower L-shaped plate (49). The side of the top end of the lower L-shaped plate (49) is mounted with a second positioning bolt (51). A sliding plate (52) is slidably mounted at the bottom end of the lower L-shaped plate (49). The sliding plate (52) is fixed to the outside of the arc-shaped clamping plate (42). A fourth positioning bolt (53) is mounted on the inner top of the lower L-shaped plate (49).

10. An oral amino acid composition for relieving ulcerative colitis, characterized in that: C57BL / 6J male mice were purchased from Guangzhou Regene Biotechnology Co., Ltd. All mice were fed with sterile water and autoclaved food in individually ventilated cages. The animals were raised under specific pathogen-free conditions at a temperature of 22 ± 2 °C, a humidity of 50 ± 5%, and a light-dark cycle of 12 h per time. The experiment was approved by the Animal Ethics Committee of the School of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine; After 1 week of adaptive feeding, the mice were randomly divided into 3 groups: normal control group Cont, model control 3% DSS group, 3% DSS + HAAs group; The control group drank sterile water, and the other groups drank 3% DSS freely. At the same time, the animals in the Cont group and Mod group were given physiological saline intervention, and the animals in the HAAs group were given corresponding concentration gavage intervention. Starting from the day before modeling, the mice were scored for the disease activity index (DAI) to evaluate the severity of colitis, including the weight change rate of the mice, the average scores of loose stools and bloody stools. On the 8th day, the animals were anesthetized with pentobarbital and the colon and whole blood of the mice were collected. Some of them were stored at -80℃, the spleen was weighed, and the spleen coefficient was calculated. The other part of the tissue was fixed with 4% paraformaldehyde for subsequent experiments.

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