Engineering strain for producing urolithin A and application thereof

By constructing a co-expression vector pET28a-UroA containing tannic acid hydrolase, esterase, gallic acid decarboxylase and dopamine dehydroxylase, the problems of complex production and unstable yield of urolithin A in the existing technology have been solved, realizing efficient and simple large-scale production of urolithin A, which is suitable for functional food raw materials.

CN121801787APending Publication Date: 2026-04-07ZHENJIANG TIANYI BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202512007244.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-29
Publication Date
2026-04-07

AI Technical Summary

Technical Problem

Existing chemical synthesis methods for preparing urolithin A are complex and unsuitable for food additives, while biosynthesis methods suffer from the influence of microbial flora on yield and the generation of various urolithins, resulting in a lack of efficient large-scale production methods.

Method used

A co-expression vector pET28a-UroA containing tannic acid hydrolase, esterase, gallic acid decarboxylase and dopamine dehydroxylase was constructed and transformed into Escherichia coli BL21 to achieve whole-cell catalysis of ellagitannin to urolithin A.

Benefits of technology

It enables efficient and simple large-scale production of urolithin A, avoiding complex extraction and purification processes, is environmentally friendly and low-cost, and is suitable for functional food ingredients.

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Abstract

The invention discloses an engineering strain for producing urolithin A and application of the engineering strain. The engineering strain contains a tannin acyl hydrolase gene tah, an esterase gene lip, a gallic acid decarboxylase gene gdo and a dopamine dehydroxylase gene ascd. The four artificially synthesized genes are jointly constructed on an escherichia coli expression vector, so that a brand-new vector containing four co-expression genes is constructed, then the brand-new vector is transferred into a chassis strain cell to realize co-expression of the four genes, and four enzymes expressed in the cell all have activity; and the obtained heavy engineering strain can stably convert the extracellular added ellagic tannin into the urolithin A, so that the whole-cell catalysis process of producing the urolithin A by taking the ellagic tannin as a substrate is realized.
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