Bone marrow cell preparation device

Abstract

The utility model relates to the technical field of bone marrow cell preparation, in particular to a bone marrow cell preparation device which comprises a bottom plate, stand columns are arranged on the two sides of the bottom plate, a culture dish is arranged on the bottom plate between the two stand columns, and rotating rods are rotationally arranged on the opposite sides of the two stand columns at the same height. The ends, away from the corresponding stand columns, of the two rotating rods are close to each other, the two rotating rods are horizontally arranged, vertical plates are arranged at the ends, close to each other, of the two rotating rods, springs are arranged on the sides, away from the corresponding rotating rods, of the vertical plates, clamping plates are arranged at the ends, away from the corresponding vertical plates, of the springs, and the two clamping plates are used for clamping bones. One rotating rod penetrates through the corresponding stand column and then is connected with a mounting plate, an abutting bolt is connected to the mounting plate, the abutting bolt penetrates through the mounting plate and then abuts against the side wall of the corresponding stand column, a top plate is arranged at the tops of the two stand columns, a flushing fluid box is arranged on the top plate and communicates with a flushing pump, and the pump-out end of the flushing pump communicates with a needle head. The bone marrow cells can be prepared more conveniently.

Description

Technical Field

[0001] The utility model relates to the technical field of bone marrow cell preparation, in particular to a bone marrow cell preparation device. Background Art

[0002] The genes on chromosomes determine all the information about the growth and development of a species. Therefore, through chromosome analysis, we can understand the most basic genetic indicators of a species. The preparation of chromosome specimens is generally taken from tissues with vigorous cell division, such as bone marrow, lymphocytes, and artificially cultured suspensions.

[0003] In cell biology experiments, leg bones of animals such as mice and pigs are often used to extract bone marrow. When extracting bone marrow from bones, culture fluid is usually drawn with a syringe, and then the needle of the culture fluid is inserted into the bone to flush it. The bone marrow in the bone is flushed into the culture vessel. In order to obtain more bone marrow cells, the bone is usually turned upside down and flushed again.

[0004] The applicant believes that in the related art, during the process of preparing and obtaining bone marrow cells, it is necessary to use a handheld syringe to flush the bone marrow multiple times, and during the extraction process, a handheld clamp is also required to fix the animal bones, which makes the process of preparing and extracting bone marrow cells more cumbersome and inconvenient. Utility Model Content

[0005] The utility model aims to provide a bone marrow cell preparation device to solve the problem that the process of preparing and extracting bone marrow cells is relatively complicated and inconvenient.

[0006] The utility model is realized by the following technical solutions:

[0007] The cam is provided with a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams, each of which has a plurality of cams,

[0008] Furthermore, two springs are provided on each of the vertical plates, one spring on the same vertical plate is located at the upper end of the corresponding vertical plate, and the other spring is located at the lower end of the corresponding vertical plate.

[0009] Furthermore, a plurality of protrusions are provided on one side of the two clamping plates facing away from the corresponding springs, and the plurality of protrusions are evenly distributed on the corresponding vertical plates.

[0010] Furthermore, the plurality of protrusions are all made of silicone material.

[0011] Compared with the prior art, the utility model has the following advantages and beneficial effects:

[0012] In the utility model, when preparing to extract bone marrow cells of an animal, the leg bone of the animal is first obtained, and then the leg bone of the animal is fixedly clamped between two clamping plates so that the leg bone is vertically arranged with the lower end facing the culture dish below, and then the flushing pump is started, and the flushing pump sends the flushing culture solution in the flushing liquid tank through the needle, and then the needle is inserted into the upper end of the leg bone to prevent air from being pushed into the leg bone and damaging the bone marrow cells. The flushing pump can automatically and continuously deliver the flushing solution into the leg bone, so that the bone marrow in the leg bone can be automatically flushed into the culture dish below for a period of time. After a period of time, turn off the flushing pump, take out the needle, rotate the locking bolt, separate the locking bolt from the column, rotate the mounting plate 180 degrees to turn the leg bone upside down, and then rotate the locking bolt so that the locking bolt is pressed against the side wall of the column, thereby fixing the two rotating rods on the two columns, and then repeating the above flushing steps to fully flush the bone marrow in the leg bone. In the process of extracting bone marrow cells, the process of manually clamping the leg bone is reduced, and the flushing pump is used instead of the step of manually injecting the leg bone, which is more convenient and labor-saving. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The drawings described herein are used to provide a further understanding of the embodiments of the present utility model, constitute a part of this application, and do not constitute a limitation of the embodiments of the present utility model.

[0014] In the attached picture:

[0015] Figure 1 This is a schematic diagram of the structure of Embodiment 1 of the present utility model, which is intended to show the state when the bone is not clamped;

[0016] Figure 2 This is a schematic diagram of the structure of Example 1 of the utility model, which is intended to show the state of clamping bones.

[0017] Marks and corresponding parts names in the attached drawings:

[0018] 1. Bottom plate; 2. Column; 3. Culture dish; 4. Rotating rod; 5. Vertical plate; 6. Spring; 7. Clamping plate; 8. Bone; 9. Mounting plate; 10. Tightening bolt; 11. Top plate; 12. Flushing liquid tank; 13. Flushing pump; 14. Needle; 15. Protrusion. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in combination with the embodiments and drawings. The schematic implementation mode and description of the utility model are only used to explain the utility model and are not intended to limit the utility model. It should be noted that the utility model is already in the actual development and use stage.

[0020] Example 1

[0021] A bone marrow cell preparation device, referring to Figure 1 , Figure 2 , including a bottom plate 1, columns 2 are arranged on both sides of the bottom plate 1, a culture dish 3 is arranged on the bottom plate 1 between the two columns 2, rotating rods 4 are rotatably arranged at the same height on the opposite sides of the two columns 2, the two rotating rods 4 are rotatably connected to the corresponding columns 2 through bearings, the ends of the two rotating rods 4 away from the corresponding columns 2 are close to each other, the two rotating rods 4 are arranged horizontally, the central axes of the two rotating rods 4 coincide with each other, and the ends of the two rotating rods 4 close to each other are arranged with vertical plates 5, the side of the vertical plates 5 away from the corresponding rotating rods 4 is provided with springs 6, and the end of the spring 6 away from the corresponding vertical plates 5 is provided with a clamping plate 7, The two clamping plates 7 are pressed against each other, and the two clamping plates 7 are used to clamp the bones 8. Any rotating rod 4 is connected to a mounting plate 9 after passing through the corresponding column 2. A tightening bolt 10 is threadedly connected to the mounting plate 9. The tightening bolt 10 is pressed against the side wall of the corresponding column 2 after passing through the mounting plate 9. A top plate 11 is arranged on the top of the two columns 2, and a flushing liquid tank 12 is arranged on the top plate 11. The flushing liquid tank 12 is connected to a flushing pump 13 through a pipeline, and a needle 14 is arranged at the pump outlet of the flushing pump 13 through a pipeline. A hose is used for the pipeline connecting the needle 14, which is convenient for inserting the needle 14 into the leg bone and removing the needle 14.

[0022] When preparing to extract bone marrow cells from animals, it is necessary to do so in a sterile environment. First, obtain the leg bone of the animal, move the clamping plate 7, and then fix the leg bone of the animal between the two clamping plates 7 so that the leg bone is vertically arranged with the lower end facing the culture dish 3 below. Then start the flushing pump 13, and the flushing pump 13 sends the flushing culture solution in the flushing liquid tank 12 through the needle 14. Then, the needle 14 is inserted into the upper end of the leg bone to prevent air from being pushed into the leg bone and damaging the bone marrow cells. The flushing pump 13 can automatically and continuously deliver the flushing solution into the leg bone, so that the bone marrow in the leg bone can be automatically flushed to the culture dish 3 below. After a period of time, turn off the flushing pump 13, take out the needle 14, rotate the tightening bolt 10, separate the tightening bolt 10 from the column 2, rotate the mounting plate 9180 degrees, so that the leg bone is turned upside down, and then rotate the tightening bolt 10 so that the tightening bolt 10 is pressed against the side wall of the column 2, so that the two rotating rods 4 are fixed on the two columns 2, and then the above-mentioned flushing steps are repeated to fully flush the bone marrow in the leg bone. In the process of extracting bone marrow cells, the process of manually clamping the leg bone is reduced, and the flushing pump 13 is used instead of the step of manually injecting the leg bone, which is more convenient and labor-saving.

[0023] As a preferred embodiment, refer to Figure 1 , Figure 2 Each vertical plate 5 is provided with two springs 6, one spring 6 on the same vertical plate 5 is located at the upper end of the corresponding vertical plate 5, and the other spring 6 is located at the lower end of the corresponding vertical plate 5. Since the upper and lower ends of the leg bone clamping part may be different in size, the upper and lower springs 6 can appropriately compensate for this difference, so that the upper and lower parts of the clamping plate 7 can be tightly pressed against the leg bone, which is convenient for clamping and fixing the leg bone more stably, and can reduce the shaking of the bone 8 during the flushing process.

[0024] As a preferred embodiment, refer to Figure 1 , Figure 2 , the two clamping plates 7 are provided with a plurality of protrusions 15 on one side away from the corresponding spring 6, and the plurality of protrusions 15 are evenly distributed on the corresponding vertical plate 5. The plurality of protrusions 15 are conducive to enhancing the friction force of the clamping surface of the clamping plate 7, so that the two clamping plates 7 can clamp the animal leg bone more stably.

[0025] As a preferred embodiment, refer to Figure 1 , Figure 2 The plurality of protrusions 15 are all made of silicone material, which has good anti-slip and wear-resistant properties, so that the clamping plate 7 can reduce the loosening and deviation of the leg bone during the process of clamping and fixing the leg bone.

[0026] The specific implementation methods described above further illustrate the purpose, technical solutions and beneficial effects of the utility model in detail. It should be understood that the above description is only the specific implementation method of the utility model and is not used to limit the protection scope of the utility model. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the utility model should be included in the protection scope of the utility model.

Claims

1. A bone marrow cell preparation device, Features: The invention comprises a bottom plate (1), both sides of the bottom plate (1) are provided with upright posts (2), a culture dish (3) is provided on the bottom plate (1) between two upright posts (2), a rotating rod (4) is rotatably provided at the same height on the opposite sides of the two upright posts (2), the central axes of the two rotating rods (4) coincide with each other, the two rotating rods (4) are arranged horizontally, and a vertical plate (5) is provided at one end of the two rotating rods (4) close to each other, a spring (6) is provided on the side of the vertical plate (5) away from the corresponding rotating rod (4), and a clamping member is provided at the end of the spring (6) away from the corresponding vertical plate (5). The two clamping plates (7) are used to clamp the bones (8); any one of the rotating rods (4) is connected to a mounting plate (9) after passing through the corresponding column (2); a tightening bolt (10) is threadedly connected to the mounting plate (9); the tightening bolt (10) is tightened against the side wall of the corresponding column (2) after passing through the mounting plate (9); a top plate (11) is arranged on the top of the two columns (2); a flushing liquid tank (12) is arranged on the top plate (11); a flushing pump (13) is arranged in communication with the flushing liquid tank (12); and a needle (14) is arranged at the pump outlet of the flushing pump (13).

2. A bone marrow cell preparation device according to claim 1, Features: Two springs (6) are arranged on each vertical plate (5); one spring (6) on the same vertical plate (5) is located at the upper end of the corresponding vertical plate (5), and the other spring (6) is located at the lower end of the corresponding vertical plate (5).

3. A bone marrow cell preparation device according to claim 1, Features: A plurality of protrusions (15) are provided on the side of the two clamping plates (7) facing away from the corresponding spring (6), and the plurality of protrusions (15) are evenly distributed on the corresponding vertical plate (5).

4. A bone marrow cell preparation device according to claim 3, Features: The plurality of protrusions (15) are all made of silicone material.

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