Serum-free cell culture dish with constant-speed oscillation effect

Abstract

The utility model relates to the technical field of stem cell differentiation, and discloses a serum-free cell culture dish with a constant-speed oscillation effect, which comprises a box body, the oscillating device is arranged on the box body and is used for oscillating the culture dishes; the clamping device is arranged on the box body and is used for clamping a culture dish; the box cover is fixedly mounted at the top of the box body; wherein the oscillation device comprises a rotating device which is arranged on the box body and is used for oscillating the culture dish; the spring device is arranged on the box body and used for providing a cushioning effect on the partition plate, the motor fixedly connected with the top of the supporting plate is started, the motor drives the rotating rod, the rotating rod drives the oval block, when the oval block rotates to the highest position, the partition plate is extruded and slides on the inner wall of the guide groove to move towards the top, and when the lowest position of the oval block rotates to the top, the partition plate moves towards the bottom. When the partition plate descends, the first spring provides buffering force for falling of the partition plate, and constant-speed oscillation of the culture dish is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell differentiation, in particular to a serum-free cell culture dish with a uniform oscillation effect. Background Art

[0002] Stem cell therapy refers to the in vitro expansion or directed differentiation of stem cells from autologous or allogeneic sources to prepare them into specific cells required for treatment.

[0003] According to the Chinese patent publication number CN212501733U, the technical solution disclosed in the patent document is as follows: The utility model discloses a serum-free culture dish for stem cell differentiation, including an incubator, a partition plate is installed inside the incubator, a mounting block is installed on one side of the partition plate and the inner wall of the incubator, a sleeve rod is installed on the end of the mounting block close to each other, a sleeve spring is installed on the outer surface of the sleeve rod, a clamping plate is installed on the side of the sleeve rod close to each other, a connecting rod is fixedly installed inside the incubator on one side of the partition plate, a mounting block is fixedly installed on one end of the connecting rod, and a card and rod are installed on the other end of the mounting block. The utility model is provided with a clamping plate, and two incubators clamped in the middle will limit the serum-free culture solution placed in the incubator by the staff, so as to prevent the serum-free culture solution from shaking and contacting with the inner wall of the incubator when the staff transports the incubator.

[0004] The above patent has the following shortcomings: the culture dish of the device needs to be vibrated when in use to increase the oxygen in the solution to meet the respiration needs of the segmented cells. The vibration can make the culture medium fully contact with oxygen, increase the supply of dissolved oxygen, and the bacteria cultured by vibration have uniform reproduction and high culture efficiency. In view of this, we propose a serum-free cell culture dish with uniform vibration effect. Utility Model Content

[0005] The utility model aims to provide a serum-free cell culture dish with uniform oscillation effect to solve the problems raised in the above background technology.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A serum-free cell culture dish with a uniform oscillation effect comprises: a box; an oscillating device, arranged on the box and used to oscillate the culture dish; a clamping device, arranged on the box and used to clamp the culture dish; a box cover, fixedly mounted on the top of the box; wherein the oscillating device comprises: a rotating device, arranged on the box and used to oscillate the culture dish; and a spring device, arranged on the box and used to provide a shock-absorbing effect to the partition.

[0008] Preferably, the rotating device includes a support plate, which is fixedly connected to the front side of the box body, and a motor is fixedly connected to the top of the support plate. A rotating rod is rotatably connected to the front side of the inner wall of the box body through a bearing, and the rotating rod movably passes through the box body and extends to the front side, and its extended end is fixedly connected to the output end of the motor. An elliptical block is fixedly provided on the outer wall of the rotating rod.

[0009] Preferably, the spring device includes a fixed plate, which is fixedly connected to the front side of the inner wall of the box, guide grooves are opened around the inner wall of the box, and the inner wall of the guide groove is slidably connected with a partition, a moving rod movably passes through the top of the fixed plate, and the top of the moving rod is fixedly connected to the bottom of the partition, and a first spring is movably sleeved on the outer wall of the moving rod, and two ends of the first spring are respectively fixedly connected to the bottom of the partition and the top of the fixed plate.

[0010] Preferably, the clamping device includes a slide groove and a fixed block, the slide groove is opened at the top of the partition, the inner wall of the slide groove is slidably connected with a slider, the top of the slider is fixedly connected with a splint, the fixed block is fixedly connected to the top of the partition, a pull rod movably passes through the right side of the fixed block, and a second spring is movably sleeved on the outer wall of the pull rod, and two ends of the second spring are respectively fixedly connected to the right side of the fixed block and the left side of the splint, and the left side of the pull rod is fixedly connected with a pull block.

[0011] Preferably, the first spring and the second spring are compression springs, which are easy to manufacture and process, have low cost, and can be applied to different shapes, sizes and load requirements, so they have high applicability. The compression spring has a compression force. The compression spring has the characteristics of high elasticity, high thermal conductivity and high durability. It has high reliability and stability during operation. Compared with ordinary springs, the design of the compression spring is more compact, which can exert a greater compression effect in a limited space and save equipment space.

[0012] Preferably, there are two elliptical blocks, which can hit the partition evenly to improve the oscillation effect.

[0013] Compared with the prior art, the utility model provides a serum-free cell culture dish with a uniform oscillation effect, which has the following beneficial effects:

[0014] 1. A serum-free cell culture dish with uniform oscillation effect, by starting a motor fixedly connected to the top of a support plate, the motor drives a rotating rod, and the rotating rod drives an elliptical block. When the elliptical block rotates to the highest point, it squeezes a partition, and the partition slides on the inner wall of a guide groove to move toward the top. When the elliptical block rotates to the top at the lowest point, when the partition descends, the first spring provides a buffering force for the partition to fall, thereby achieving uniform oscillation of the culture dish.

[0015] 2. The serum-free cell culture dish with uniform oscillation effect is achieved by opening the box cover and pulling the pull block, which drives the pull rod to move to the right, and the pull rod drives the clamp to move to the right. At the same time, when the clamp moves, it drives the slider fixed at the bottom to slide in the inner wall of the slide groove to prevent the clamp from deviating from the original moving track during movement, thereby improving the convenience of taking out the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0017] Figure 2 It is a schematic diagram of the right side cross-sectional structure of the utility model;

[0018] Figure 3 This is a schematic diagram of the top cross-sectional structure of the utility model;

[0019] Figure 4 It is a front sectional structural schematic diagram of the utility model.

[0020] In the figure: 1. box body; 2. oscillating device; 21. rotating device; 211. supporting plate; 212. motor; 213. rotating rod; 214. elliptical block; 22. spring device; 221. fixed plate; 222. moving rod; 223. first spring; 224. partition; 3. clamping device; 301. slide groove; 302. slider; 303. fixed block; 304. pulling block; 305. clamping plate; 306. pulling rod; 307. second spring; 4. box cover. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] In the description of the present invention, it should be understood that the terms "center", "length", "up", "down", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0023] In the description of this patent, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "setting" should be understood in a broad sense, for example, it can be fixed connection, setting, or detachable connection, setting, or integral connection, setting. For ordinary technicians in this field, the specific meanings of the above terms in this patent can be understood according to specific circumstances.

[0024] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, the meaning of "several" is two or more, unless otherwise clearly and specifically defined.

[0025] According to the Chinese patent publication number CN212501733U, the technical solution disclosed in the patent document is as follows: The utility model discloses a serum-free culture dish for stem cell differentiation, including an incubator, a partition plate is installed inside the incubator, a mounting block is installed on one side of the partition plate and the inner wall of the incubator, a sleeve rod is installed on the end of the mounting block close to each other, a sleeve spring is installed on the outer surface of the sleeve rod, a clamping plate is installed on the side of the sleeve rod close to each other, a connecting rod is fixedly installed inside the incubator on one side of the partition plate, a mounting block is fixedly installed on one end of the connecting rod, and a card and rod are installed on the other end of the mounting block. The utility model is provided with a clamping plate, and two incubators clamped in the middle will limit the serum-free culture solution placed in the incubator by the staff, so as to prevent the serum-free culture solution from shaking and contacting with the inner wall of the incubator when the staff transports the incubator.

[0026] The above patent has the following shortcomings: the culture dish of the device needs to be vibrated when in use to increase the oxygen in the solution to meet the respiration needs of the segmented cells. The vibration can make the culture medium fully contact with oxygen, increase the supply of dissolved oxygen, and the bacteria cultured by vibration have uniform reproduction and high culture efficiency. In view of this, we propose a serum-free cell culture dish with uniform vibration effect. Example 1

[0027] like Figure 1-4 As shown, a serum-free cell culture dish with uniform oscillation effect includes a box body 1; an oscillating device 2, which is arranged on the box body 1 and is used to oscillate the culture dish; a clamping device 3, which is arranged on the box body 1 and is used to clamp the culture dish; a box cover 4, which is fixedly installed on the top of the box body 1; wherein the oscillating device 2 includes: a rotating device 21, which is arranged on the box body 1 and is used to oscillate the culture dish; a spring device 22, which is arranged on the box body 1 and is used to provide a shock-absorbing effect to the partition 224.

[0028] The rotating device 21 includes a support plate 211, which is fixedly connected to the front of the box body 1. A motor 212 is fixedly connected to the top of the support plate 211. A rotating rod 213 is rotatably connected to the front of the inner wall of the box body 1 through a bearing, and the rotating rod 213 movably penetrates the box body 1 and extends toward the front. The extended end is fixedly connected to the output end of the motor 212, and an elliptical block 214 is fixedly sleeved on the outer wall of the rotating rod 213.

[0029] The spring device 22 includes a fixed plate 221, which is fixedly connected to the front side of the inner wall of the box body 1. Guide grooves are provided around the inner wall of the box body 1, and a partition 224 is slidably connected to the inner wall of the guide groove. A moving rod 222 movably penetrates the top of the fixed plate 221, and the top of the moving rod 222 is fixedly connected to the bottom of the partition 224. A first spring 223 is movably sleeved on the outer wall of the moving rod 222, and the two ends of the first spring 223 are respectively fixedly connected to the bottom of the partition 224 and the top of the fixed plate 221.

[0030] The first spring 223 and the second spring 307 are compression springs, which are easy to manufacture and process, have low cost, and can be applied to different shapes, sizes and load requirements. Therefore, they have high applicability. The compression spring has a compression force. The compression spring has the characteristics of high elasticity, high thermal conductivity and high durability. It has high reliability and stability during operation. Compared with ordinary springs, the design of the compression spring is more compact, which can exert a greater compression effect in a limited space and save equipment space.

[0031] There are two elliptical blocks 214, which can evenly hit the partition 224 to improve the oscillation effect.

[0032] In this embodiment, by starting the motor 212 fixedly connected to the top of the support plate 211, the motor 212 drives the rotating rod 213, and the rotating rod 213 drives the elliptical block 214. When the elliptical block 214 rotates to the highest point, it will squeeze the partition 224, and the partition 224 slides on the inner wall of the guide groove and moves toward the top. When the elliptical block 214 rotates to the top from the lowest point, when the partition 224 descends, the first spring 223 provides it with a buffering force for the partition 224 to fall, thereby realizing uniform oscillation of the culture dish. Example 2

[0033] like Figure 1-4As shown, the clamping device 3 includes a slide groove 301 and a fixed block 303. The slide groove 301 is opened at the top of the partition 224. The inner wall of the slide groove 301 is slidably connected with a slider 302. The top of the slider 302 is fixedly connected with a splint 305. The fixed block 303 is fixedly connected to the top of the partition 224. A pull rod 306 is movably passed through the right side of the fixed block 303, and a second spring 307 is movably sleeved on the outer wall of the pull rod 306. The two ends of the second spring 307 are respectively fixedly connected to the right side of the fixed block 303 and the left side of the splint 305. The left side of the pull rod 306 is fixedly connected with the pull block 304.

[0034] In this embodiment, by opening the box cover 4 and pulling the pull block 304, the pull block 304 drives the pull rod 306 to move to the right, and the pull rod 306 drives the splint 305 to move to the right. At the same time, when the splint 305 moves, it drives the slider 302 fixed at the bottom to slide in the inner wall of the slide groove 301, preventing the splint 305 from deviating from the original moving trajectory during movement, thereby improving the convenience of taking out the culture dish.

[0035] The working principle of the utility model is as follows: open the box cover 4, pull the pull block 304, the pull block 304 drives the pull rod 306 to move to the right, and the pull rod 306 drives the clamping plate 305 to move to the right. At the same time, when the clamping plate 305 moves, it drives the slider 302 fixedly connected at the bottom to slide in the inner wall of the slide groove 301 to prevent the clamping plate 305 from deviating from the original moving trajectory during movement, thereby improving the convenience of taking and placing the culture dish, and starting the motor 212 fixedly connected to the top of the support plate 211. The motor 212 drives the rotating rod 213, and the rotating rod 213 drives the elliptical block 214. When the elliptical block 214 rotates to the highest point, it will squeeze the partition 224, and the partition 224 slides on the inner wall of the guide groove and moves to the top. When the elliptical block 214 rotates to the top at the lowest point, when the partition 224 descends, the first spring 223 provides it with a buffering force for the partition 224 to fall, thereby realizing uniform oscillation of the culture dish.

[0036] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the utility model and are not used to limit the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection of the utility model is defined by the attached claims and their equivalents.

Claims

1. A serum-free cell culture dish with uniform oscillation effect, characterized in that: include: Box (1); An oscillating device (2), arranged on the box (1) and used to oscillate the culture dish; A clamping device (3), arranged on the box (1) and used for clamping the culture dish; A box cover (4) is fixedly mounted on the top of the box body (1); Wherein, the oscillating device (2) comprises: A rotating device (21), arranged on the box (1) and used for shaking the culture dish; A spring device (22) is arranged on the box body (1) and is used to provide a shock absorbing effect for the partition plate (224).

2. A serum-free cell culture dish with uniform oscillation effect according to claim 1, characterized in that: The rotating device (21) comprises a support plate (211), the support plate (211) being fixedly connected to the front of the box body (1), the top of the support plate (211) being fixedly connected to a motor (212), the front of the inner wall of the box body (1) being rotatably connected to a rotating rod (213) via a bearing, the rotating rod (213) movably passes through the box body (1) and extends toward the front, the extended end of which is fixedly connected to the output end of the motor (212), and the outer wall of the rotating rod (213) is fixedly sleeved with an elliptical block (214).

3. The serum-free cell culture dish with uniform oscillation effect according to claim 1, characterized in that: The spring device (22) comprises a fixed plate (221), the fixed plate (221) being fixedly connected to the front side of the inner wall of the box body (1), the inner wall of the box body (1) being provided with guide grooves around its periphery, and the inner wall of the guide groove being slidably connected to a partition (224), a moving rod (222) movably passing through the top of the fixed plate (221), and the top of the moving rod (222) being fixedly connected to the bottom of the partition (224), a first spring (223) being movably sleeved on the outer wall of the moving rod (222), and two ends of the first spring (223) being fixedly connected to the bottom of the partition (224) and the top of the fixed plate (221), respectively.

4. The serum-free cell culture dish with uniform oscillation effect according to claim 2, characterized in that: The clamping device (3) comprises a slide groove (301) and a fixed block (303), wherein the slide groove (301) is opened at the top of the partition (224), the inner wall of the slide groove (301) is slidably connected to a slider (302), the top of the slider (302) is fixedly connected to a clamp (305), the fixed block (303) is fixedly connected to the top of the partition (224), a pull rod (306) movably penetrates the right side of the fixed block (303), and a second spring (307) is movably sleeved on the outer wall of the pull rod (306), and two ends of the second spring (307) are respectively fixedly connected to the right side of the fixed block (303) and the left side of the clamp (305), and the left side of the pull rod (306) is fixedly connected to the pull block (304).

5. The serum-free cell culture dish with uniform oscillation effect according to claim 3, characterized in that: The first spring (223) and the second spring (307) are compression springs.

6. The serum-free cell culture dish with uniform oscillation effect according to claim 2, characterized in that: There are two elliptical blocks (214).

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