Instrument convenient for observing coating of magnetic bead antibody

Abstract

The utility model discloses an instrument convenient for observing the coating of a magnetic bead antibody, which comprises a cylinder body, a feed hopper arranged at the upper end of the cylinder body, a processing assembly arranged at the lower end of the cylinder body, an observation assembly arranged on one side of the cylinder body and partially positioned in the cylinder body, and a control assembly arranged on the other side of the cylinder body, one end of the cylinder is connected with an output pipe, by arranging the processing assembly, the incubation and combination efficiency of the magnetic beads, antibodies and a sealing agent can be guaranteed in the coating and combination process of the magnetic beads, the antibodies and the sealing agent, so that the subsequent use effect can be facilitated, manual coating is avoided, automation of magnetic bead coating is guaranteed, and the production efficiency of the magnetic beads is improved. By arranging the observation assembly, the coating binding rate of the coated magnetic beads can be observed in the coating process of the magnetic beads, and if incubation binding is not complete, the observation assembly can be matched with the processing assembly, so that real-time observation of incubation binding between the magnetic beads and antibodies can be better ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of chemiluminescent immunoassay, in particular to an instrument for conveniently observing magnetic bead antibody coating. Background Art

[0002] Magnetic bead antibody coating is a technology that fixes antibodies to the surface of magnetic particles. It is widely used in biomedical research and clinical diagnosis. The observation of magnetic bead antibody coating usually involves the detection of whether the magnetic bead surface is successfully bound to the antibody, as well as the evaluation of the stability and functionality of the magnetic beads after coating. In order to facilitate the observation and evaluation of the effect of magnetic bead antibody coating, an instrument that is convenient for observing magnetic bead antibody coating is needed.

[0003] In the process of magnetic bead antibody coating, the existing coating methods are mostly performed manually, which makes the coating efficiency low, and the coated magnetic beads and antibodies cannot be observed during the coating process, so the binding rate between the magnetic beads and the antibodies cannot be observed in real time.

[0004] Currently, no effective solution has been proposed for the problems in the related technologies. Utility Model Content

[0005] 1. Technical issues to be solved

[0006] In view of the deficiencies of the prior art, the utility model provides an instrument for conveniently observing magnetic bead antibody coating, which has the advantages of the above-mentioned technical problems and thus solves the problems in the prior art.

[0007] (II) Technical solution

[0008] In order to achieve the advantages of the above technical problems, the specific technical solutions adopted by the utility model are as follows:

[0009] An instrument for conveniently observing magnetic bead antibody coating comprises a barrel, a feed hopper is arranged at the upper end of the barrel, a processing assembly is arranged at the lower end of the barrel, one end of the processing assembly extends from the outside of the barrel to the inside of the barrel, an observation assembly is arranged on one side of the barrel, and part of the observation assembly is located inside the barrel, and one end of the barrel is connected to an output tube.

[0010] Furthermore, in order to better ensure the coating efficiency between the magnetic beads and the antibodies, the processing component includes a first motor, which is arranged at one end of the cylinder by bolts, and the output shaft of the first motor is connected to a processing rod through a coupling, and one end of the processing rod extends from the outside of the cylinder to the inside of the cylinder, and a plurality of stirring rods are arranged at one end of the processing rod located inside the cylinder.

[0011] Furthermore, the stirring rod is configured as a hollow structure, a heating tube is provided inside the stirring rod, a plurality of conduction columns are provided on the stirring rod, a plurality of temperature sensors are provided on the processing rod, a protective shell is provided around the first motor, and one end of the protective shell is provided at one end of the cylinder, one end of the output pipe extends from the inside of the protective shell to the outside of the protective shell, a cleaning scraper is provided at one end of the stirring rod, and the cleaning scraper is in contact with the inner wall of the cylinder.

[0012] Furthermore, in order to better deliver the washing solution, antibodies and blocking agents, thereby ensuring the coating efficiency of the magnetic beads, the processing component also includes a delivery pump, which is fixedly arranged on one side of the cylinder, and the output end of the delivery pump is connected to a delivery pipe, and a plurality of output nozzles are connected to the delivery pipe, one end of the output nozzle extends from the outside of the cylinder to the inside of the cylinder, and the input end of the delivery pump is connected to an input pipe.

[0013] Furthermore, in order to better observe the binding of the coating, the observation component includes a plurality of movable grooves, which are staggered on the cylinder, a transparent plate is arranged in the movable groove, a spectrometer is arranged on one side of the transparent plate, and a movable frame is arranged on one side of the spectrometer.

[0014] Furthermore, a second motor is arranged at one end of the movable frame through the fixed frame, an adjusting gear is arranged on the output shaft of the second motor, an adjusting rack is meshedly connected to the adjusting gear, and the adjusting rack is arranged on the cylinder.

[0015] Furthermore, a moving block is arranged at the other end of the moving frame, an auxiliary groove is arranged on the cylinder, the moving block is arranged in the auxiliary groove, an observation window is arranged on the cylinder, and the observation window is located directly below one of the moving frames.

[0016] (III) Beneficial effects

[0017] Compared with the prior art, the utility model has the following beneficial effects: by setting up a processing component, the efficiency of incubation and binding of magnetic beads with antibodies and blocking agents can be ensured during the process of coating and binding of magnetic beads with antibodies and blocking agents, thereby facilitating subsequent use effects, thereby avoiding manual coating and ensuring the automation of magnetic bead coating; by setting up an observation component, the coating binding rate of the coated magnetic beads can be observed during the magnetic bead coating process; if the incubation and binding is relatively incomplete, it can be better ensured by cooperating with the processing component to better ensure real-time observation of the incubation and binding between the magnetic beads and the antibody. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 The structure of an instrument for observing the coating of magnetic beads and antibodies according to an embodiment of the utility model is shown in FIG. Figure 1 ;

[0020] Figure 2 The structure of an instrument for observing the coating of magnetic beads and antibodies according to an embodiment of the utility model is shown in FIG. Figure 2 ;

[0021] Figure 3 This is a schematic diagram of the cross-sectional structure of an instrument for conveniently observing magnetic bead antibody coating according to an embodiment of the utility model;

[0022] Figure 4 The schematic diagram of the structure of an instrument processing component for conveniently observing magnetic bead antibody coating according to an embodiment of the utility model is as follows Figure 1 ;

[0023] Figure 5 The schematic diagram of the structure of an instrument processing component for conveniently observing magnetic bead antibody coating according to an embodiment of the utility model is as follows Figure 2 ;

[0024] Figure 6 The schematic diagram of the structure of an instrument processing component for conveniently observing magnetic bead antibody coating according to an embodiment of the utility model is as follows Figure 3 ;

[0025] Figure 7 The present invention is a schematic diagram of an instrument for observing the structure of an antibody coating on magnetic beads. Figure 1 ;

[0026] Figure 8 The present invention is a schematic diagram of an instrument for observing the structure of an antibody coating on magnetic beads. Figure 2 .

[0027] In the figure:

[0028] 1. Cylinder; 2. Feed hopper; 3. Processing assembly; 301. First motor; 302. Processing rod; 303. Stirring rod; 304. Heating tube; 305. Conducting column; 306. Delivery pump; 307. Delivery tube; 308. Output nozzle; 309. Input tube; 4. Observation assembly; 401. Moving trough; 402. Transparent plate; 403. Spectrometer; 404. Moving frame; 405. Second motor; 406. Adjusting gear; 407. Adjusting rack; 408. Moving block; 5. Output tube; 6. Temperature sensor; 7. Protective shell; 8. Cleaning scraper; 9. Auxiliary trough; 10. Observation window. DETAILED DESCRIPTION

[0029] To further illustrate the technical solution of the present application, the present invention provides drawings, which are part of the disclosure of the present invention and are mainly used to illustrate the implementation methods. They can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the implementation methods. With reference to these contents, ordinary technicians in this field should be able to understand other possible implementation methods and advantages of the present invention. The components in the figures are not drawn to scale, and similar component symbols are generally used to represent similar components.

[0030] The utility model is further described in conjunction with the accompanying drawings and specific embodiments. Figure 1 - Figure 8 As shown, according to an embodiment of the utility model, an instrument for conveniently observing the coating of magnetic beads with antibodies comprises a barrel 1, a feed hopper 2 is arranged at the upper end of the barrel 1 for conveying and guiding the magnetic beads, a processing component 3 is arranged at the lower end of the barrel 1 for coating the magnetic beads, one end of the processing component 3 extends from the outside of the barrel 1 to the inside of the barrel 1, an observation component 4 is arranged on one side of the barrel 1 for observing the coating effect of the magnetic beads, and part of the observation component 4 is located inside the barrel 1, and one end of the barrel 1 is connected to an output tube 5 for outputting the magnetic beads coated with antibodies.

[0031] like Figure 1 - Figure 8As shown, the processing assembly 3 includes a first motor 301, which is arranged at one end of the cylinder 1 by bolts, and the output shaft of the first motor 301 is connected to a processing rod 302 through a coupling, and one end of the processing rod 302 extends from the outside of the cylinder 1 to the inside of the cylinder 1, and six groups of stirring rods 303 are arranged at one end of the processing rod 302 located inside the cylinder 1, wherein three groups are arranged in an opposite staggered manner to the other three groups, and each group of stirring rods 303 is arranged in four, and the stirring rods 303 are arranged in a hollow structure, and a heating tube 304 is arranged inside the stirring rod 303, and ten conductive columns 305 made of a metal material with good conductivity are arranged on the stirring rod 303, and twenty temperature sensors 6 are arranged on the processing rod 302, and a protective shell 7 is arranged on the periphery of the first motor 301, and one end of the protective shell 7 is arranged at one end of the cylinder 1, and one end of the output pipe 5 extends from the inside of the protective shell 7 to the outside of the protective shell 7, and a cleaning scraper 8 is arranged at one end of the stirring rod 303, and the cleaning scraper 8 is in contact with the inner wall of the cylinder 1;

[0032] The processing component 3 also includes a delivery pump 306, which is fixedly arranged on one side of the cylinder 1. The output end of the delivery pump 306 is connected to a delivery pipe 307, and fourteen output nozzles 308 are connected to the delivery pipe 307. One end of the output nozzle 308 extends from the outside of the cylinder 1 to the inside of the cylinder 1. The input end of the delivery pump 306 is connected to an input pipe 309.

[0033] Specifically, when the magnetic beads need to be coated with antibodies or blocking agents, the magnetic beads are placed into the cylinder 1 through the feed hopper 2, and then the external controller starts the first motor 301 and turns on the temperature sensor 6 and the heating tube 304. Then the output shaft of the first motor 301 drives the processing rod 302 to rotate, and also drives the five groups of stirring rods 303 to rotate during the rotation. Then the delivery pump 306 is started by the external controller, and then the input end of the delivery pump 306 inputs the antibody into the delivery tube 307 through the input tube 309 for diversion, and then the antibody is sprayed onto the rotating magnetic beads through the output nozzle 308. Then, the heating tube 304 and the conductive column 305 cooperate with each other to incubate the antibody-coated magnetic beads at room temperature for a period of time to allow the antibody or blocking agent to bind to the magnetic beads. After the binding is completed, the combined magnetic beads are washed by the cooperation of the delivery pump 306, the input tube 309, the delivery tube 307, and the output nozzle 308 to remove unbound antibodies or impurities.

[0034] like Figure 1 - Figure 8 As shown, the observation assembly 4 includes two moving grooves 401, the moving grooves 401 are staggered on the cylinder 1, a transparent plate 402 is arranged in the moving groove 401, and a spectrometer 403 is arranged on one side of the transparent plate 402. The principle of the spectrometer 403 is consistent with the principle of the direct reading spectrometer model XL2980;

[0035] A moving frame 404 is provided on one side of the spectrometer 403, and a second motor 405 is provided on one end of the moving frame 404 through a fixed frame, an adjusting gear 406 is provided on the output shaft of the second motor 405, an adjusting rack 407 is meshed and connected to the adjusting gear 406, and the adjusting rack 407 is provided on the cylinder 1, and a moving block 408 is provided on the other end of the moving frame 404, an auxiliary groove 9 is provided on the cylinder 1, and the moving block 408 is provided in the auxiliary groove 9, and an observation window 10 is provided on the cylinder 1, and the observation window 10 is located directly below one of the moving frames 404.

[0036] Specifically, during the magnetic bead coating process, the second motor 405 is started by an external controller, and then the output shaft of the second motor 405 drives the adjusting gear 406 and the adjusting rack 407 as well as the moving block 408 and the auxiliary slot 9 to engage with each other, thereby driving the moving frame 404 and the spectrometer 403 to move in the moving slot 401, so that the coated magnetic beads can be subjected to spectral analysis technology, and then the chemical properties of the surface of the magnetic beads can be analyzed, thereby inferring the state of the magnetic beads and better observing the antibody coating state of the magnetic beads.

[0037] In order to facilitate understanding of the above technical solution of the present invention, the working principle or operation method of the present invention in the actual process is described in detail below.

[0038] Working principle: In summary, with the help of the above technical solution of the utility model, when it is necessary to coat the magnetic beads with antibodies or blocking agents, the magnetic beads are placed into the cylinder 1 through the feed hopper 2, and then the external controller starts the first motor 301 and turns on the temperature sensor 6 and the heating tube 304. Then the output shaft of the first motor 301 drives the processing rod 302 to rotate, and also drives the five groups of stirring rods 303 to rotate during the rotation. Then the delivery pump 306 is started by the external controller, and then the input end of the delivery pump 306 inputs the antibody into the delivery tube 307 through the input tube 309 for diversion, and then the antibody is sprayed onto the rotating magnetic beads through the output nozzle 308. Then, the magnetic beads coated with antibodies can be incubated at room temperature for a period of time through the cooperation of the heating tube 304 and the conductive column 305, so that the antibodies or blocking agents can be combined with the magnetic beads. After the combination is completed, the combined magnetic beads are washed by the cooperation of the delivery pump 306, the input tube 309, the delivery tube 307, and the output nozzle 308 to remove unbound antibodies or impurities.

[0039] During the magnetic bead coating process, the second motor 405 is started through an external controller, and then the output shaft of the second motor 405 drives the adjusting gear 406 and the adjusting rack 407 as well as the moving block 408 and the auxiliary slot 9 to engage with each other, driving the moving frame 404 and the spectrometer 403 to move in the moving slot 401, so that the coated magnetic beads can be subjected to spectral analysis technology, and then the chemical properties of the surface of the magnetic beads can be analyzed, thereby inferring the state of the magnetic beads and better observing the antibody coating state of the magnetic beads.

[0040] In the present utility model, unless otherwise clearly stipulated and limited, the terms such as "installation", "setting", "connection", "fixation" and "screw-on" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. Unless otherwise clearly defined, ordinary technicians in this field can understand the specific meanings of the above terms in the utility model according to the specific circumstances.

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An instrument for conveniently observing magnetic bead antibody coating, comprising a barrel (1), characterized in that: A feed hopper (2) is provided at the upper end of the cylinder (1), a processing assembly (3) is provided at the lower end of the cylinder (1), one end of the processing assembly (3) extends from the outside of the cylinder (1) to the inside of the cylinder (1), an observation assembly (4) is provided on one side of the cylinder (1), and part of the observation assembly (4) is located inside the cylinder (1), and one end of the cylinder (1) is connected to an output pipe (5); The processing assembly (3) comprises a first motor (301), the first motor (301) is arranged at one end of the cylinder (1) by means of bolts, the output shaft of the first motor (301) is connected to a processing rod (302) by means of a coupling, one end of the processing rod (302) extends from the outside of the cylinder (1) to the inside of the cylinder (1), and a plurality of stirring rods (303) are arranged at one end of the processing rod (302) located inside the cylinder (1).

2. The instrument for observing magnetic bead antibody coating according to claim 1, characterized in that: The stirring rod (303) is configured as a hollow structure, a heating tube (304) is provided inside the stirring rod (303), a plurality of conduction columns (305) are provided on the stirring rod (303), a plurality of temperature sensors (6) are provided on the processing rod (302), a protective shell (7) is provided on the periphery of the first motor (301), and one end of the protective shell (7) is provided at one end of the cylinder (1), one end of the output tube (5) extends from the inside of the protective shell (7) to the outside of the protective shell (7), a cleaning scraper (8) is provided at one end of the stirring rod (303), and the cleaning scraper (8) is in contact with the inner wall of the cylinder (1).

3. The instrument for conveniently observing magnetic bead antibody coating according to claim 2, characterized in that: The processing assembly (3) further comprises a delivery pump (306), wherein the delivery pump (306) is fixedly arranged on one side of the barrel (1), wherein the output end of the delivery pump (306) is connected to a delivery pipe (307), wherein the delivery pipe (307) is connected to a plurality of output nozzles (308), wherein one end of the output nozzle (308) extends from the outside of the barrel (1) to the inside of the barrel (1), and the input end of the delivery pump (306) is connected to an input pipe (309).

4. The instrument for conveniently observing magnetic bead antibody coating according to claim 1, characterized in that: The observation assembly (4) comprises a plurality of movable grooves (401), wherein the movable grooves (401) are arranged in a staggered manner on the cylinder (1), a transparent plate (402) is arranged in the movable groove (401), a spectrometer (403) is arranged on one side of the transparent plate (402), and a movable frame (404) is arranged on one side of the spectrometer (403).

5. The instrument for conveniently observing magnetic bead antibody coating according to claim 4, characterized in that: A second motor (405) is arranged at one end of the movable frame (404) via a fixed frame, an adjusting gear (406) is arranged on the output shaft of the second motor (405), an adjusting rack (407) is meshedly connected to the adjusting gear (406), and the adjusting rack (407) is arranged on the cylinder (1).

6. The instrument for conveniently observing magnetic bead antibody coating according to claim 5, characterized in that: A moving block (408) is provided at the other end of the moving frame (404), an auxiliary groove (9) is provided on the cylinder (1), and the moving block (408) is arranged in the auxiliary groove (9), and an observation window (10) is provided on the cylinder (1), and the observation window (10) is located directly below one of the moving frames (404).

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