Application of cornflower-3-O-galactoside in preparing foods or medicaments for improving cognitive functions of old people

Abstract

The invention discloses an application of cornflower-3-O-galactoside in preparing foods or medicines for improving cognitive functions of old people. Results from animal experiments show that: compared with a control group (mice are fed with distilled water), the step-through latencies of animals fed with the cornflower-3-O-galactoside are all evidently prolonged; the escape latencies of mice in Morris water maze are evidently shortened; the duration time on a platform quadrant is evidently prolonged, and times of crossing the platform are evidently increased. The hint shows that the spatial memory ability of the old-aged mice compensated with the cornflower-3-O-galactoside can be evidently improved. Compared with an H2O2 model group, the LDH activity of hippocampal nerve cell supernate inthe group fed with the cornflower-3-O-galactoside evidently decreases and the nerve cells apoptosis rate evidently decreases. The hint shows that the cornflower-3-O-galactoside can evidently suppressthe hippocampal nerve cell apoptosis induced by H2O2 and play a protection role in the hippocampal nerve cells damaged by H2O2.

Description

technical field

[0001] The present invention relates to the use of cyanidin-3-O-galactoside, in particular to the application of cyanidin-3-O-galactoside in the preparation of food or medicine for improving the cognitive function of the elderly. Background technique

[0002] my country has entered an aging society, and the incidence of cognitive decline and neurodegenerative diseases in the elderly is increasing, which poses a huge threat to the physical and mental health and quality of life of the elderly. Epidemiological data show that there are currently about 6 million Alzheimer's patients in my country. In 2000, a survey of 5,055 elderly people over the age of 55 in the urban area of ​​Shanghai showed that the prevalence rate of Alzheimer's disease reached 4.1%. Cognitive impairment is the early stage of Alzheimer's disease. In 2006, a survey of 773 elderly people over 60 years old in the community showed that the incidence of cognitive impairment was 16.8%. Therefore...

Examples

Embodiment 1

[0020] Effect evaluation: Experimental study of cyanidin-3-O-galactoside in improving cognitive function in aged mice

[0021] There were 14 15-month-old SPF Kunming mice, half male and half male. After adaptive feeding for 1 week, they were divided into control group and cyanidin-3-O-galactoside group (monomer group) according to body weight, with 7 animals in each group. Mice in the control group were given distilled water; the dose of monomer group was 50 mg / kg bw·d. The experimental period is 6w.

[0022] The experimental results showed that (1) compared with the control group, the dark avoidance latency of the mice in the monomer group was significantly prolonged; (2) compared with the control group, the escape latency of the mice in the monomer group was significantly shortened in the Morris water maze; The duration of the quadrant is significantly extended, and the number of crossing platforms is significantly increased. It is suggested that the supplementation of cy...

Embodiment 2

[0027] Efficacy Evaluation: Experimental Study on the Reduction of Peroxidative Damage of Hippocampal Nerve Cells by Cyanide-3-O-Galactoside

[0028] Isolate and culture the hippocampal neurons of newborn suckling mice, add 50μmol / LH 2 o 2 Establishment of oxidative stress injury model (H 2 o 2 Model group); 1 μg / ml cyanidin-3-O-galactoside (monomer group) was added to the culture medium. The LDH activity, neuronal survival rate and apoptosis rate in the cell supernatant were measured.

[0029] The results showed that, with H 2 o 2 Compared with the model group, the LDH activity in the supernatant of hippocampal nerve cells in the monomer group was significantly reduced; the apoptosis rate of nerve cells was significantly reduced. Prompt cyanidin-3-O-galactoside can significantly inhibit H 2 o 2 Induced apoptosis of hippocampal neurons, on H 2 o 2 Injury to hippocampal nerve cells has a protective effect.

Embodiment 3

[0031] The cyanidin-3-O-galactoside and pharmaceutically acceptable auxiliary materials are prepared into convenient effervescent tablets, chewable tablets, capsules or injections according to conventional techniques.

technical field

[0001] The present invention relates to the use of cyanidin-3-O-galactoside, in particular to the application of cyanidin-3-O-galactoside in the preparation of food or medicine for improving the cognitive function of the elderly. Background technique

[0002] my country has entered an aging society, and the incidence of cognitive decline and neurodegenerative diseases in the elderly is increasing, which poses a huge threat to the physical and mental health and quality of life of the elderly. Epidemiological data show that there are currently about 6 million Alzheimer's patients in my country. In 2000, a survey of 5,055 elderly people over the age of 55 in the urban area of ​​Shanghai showed that the prevalence rate of Alzheimer's disease reached 4.1%. Cognitive impairment is the early stage of Alzheimer's disease. In 2006, a survey of 773 elderly people over 60 years old in the community showed that the incidence of cognitive impairment was 16.8%. Therefore...

Examples

Embodiment 1

[0020] Effect evaluation: Experimental study of cyanidin-3-O-galactoside in improving cognitive function in aged mice

[0021] There were 14 15-month-old SPF Kunming mice, half male and half male. After adaptive feeding for 1 week, they were divided into control group and cyanidin-3-O-galactoside group (monomer group) according to body weight, with 7 animals in each group. Mice in the control group were given distilled water; the dose of monomer group was 50 mg / kg bw·d. The experimental period is 6w.

[0022] The experimental results showed that (1) compared with the control group, the dark avoidance latency of the mice in the monomer group was significantly prolonged; (2) compared with the control group, the escape latency of the mice in the monomer group was significantly shortened in the Morris water maze; The duration of the quadrant is significantly extended, and the number of crossing platforms is significantly increased. It is suggested that the supplementation of cy...

Embodiment 2

[0027] Efficacy Evaluation: Experimental Study on the Reduction of Peroxidative Damage of Hippocampal Nerve Cells by Cyanide-3-O-Galactoside

[0028] Isolate and culture the hippocampal neurons of newborn suckling mice, add 50μmol / LH 2 o 2 Establishment of oxidative stress injury model (H 2 o 2 Model group); 1 μg / ml cyanidin-3-O-galactoside (monomer group) was added to the culture medium. The LDH activity, neuronal survival rate and apoptosis rate in the cell supernatant were measured.

[0029] The results showed that, with H 2 o 2 Compared with the model group, the LDH activity in the supernatant of hippocampal nerve cells in the monomer group was significantly reduced; the apoptosis rate of nerve cells was significantly reduced. Prompt cyanidin-3-O-galactoside can significantly inhibit H 2 o 2 Induced apoptosis of hippocampal neurons, on H 2 o 2 Injury to hippocampal nerve cells has a protective effect.

Embodiment 3

[0031] The cyanidin-3-O-galactoside and pharmaceutically acceptable auxiliary materials are prepared into convenient effervescent tablets, chewable tablets, capsules or injections according to conventional techniques.

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