Preparation method for decolorized blood globulin powder

Abstract

The invention discloses a preparation method for decolorized blood globulin powder. The method comprises the following steps: sampling fresh blood of a health animal; adding an anticoagulant to carry out anticoagulation, and separating to obtain a blood globulin liquid; adding a salt solution with the concentration of 0.3-0.6% to the blood globulin liquid, wherein the amount of the salt solution is 1-4 times the amount of the blood globulin liquid; carrying out high speed stirring for 1-2 hours, and heating to a temperature of 60-70 DEG C by water bath; adding an antifoaming agent to the heated blood globulin liquid, wherein the volume of the antifoaming agent is 1.0-5.0% of the volume of the blood globulin liquid; immediately adding a calcium peroxide solution with the concentration of 0.5-1.5 g / ml, and completely stirring, wherein the volume of the calcium peroxide solution is 2.0-6.0% of the volume of the blood globulin liquid; adopting hydrochloric acid to adjust the pH value to 3.5-5.5, and adopting a 10% sodium hydroxide solution to adjust the pH value to 6.5-7.5; carrying out spray drying to obtain the decolorized blood globulin powder. The method of the present invention has characteristics of simple operation and low cost, and is applicable for the large scale industrial production.

Description

Technical field:

[0001] The invention discloses a blood cell decolorization method, which is applicable to a preparation method of decolorized blood globulin powder, and the method is suitable for companies that produce animal blood deep processing products on a large scale to decolorize blood cells in animal blood raw materials. Background technique:

[0002] Our country's animal husbandry is developed, and the slaughtering volume of live pigs accounts for 50% of the world's total, and a large amount of pig blood resources are produced every year. Pig blood is rich in nutrients, among which the protein content is as high as more than 18%, and the protein is rich in immunoglobulin, with a content of 22%. The amino acid content is rich and the ratio of various amino acids is relatively balanced. In addition, pig blood also contains various active substances such as trace elements, vitamins, and hormones. At this stage, the production of plasma protein powder and blood globul...

Examples

Embodiment 1

[0024] Take 30kg of pig blood, centrifuge, take 10kg of blood cell solution, add 10kg of 0.3% salt solution, then stir the mixed solution under high-speed stirring for 1.0h, and then heat to 65°C. After taking it out, add 200ml of defoamer to eliminate the bubbles in the mixture and prevent a large number of bubbles generated during oxidation, then immediately add 400ml of 1.5g / ml calcium peroxide solution, adjust the pH of the mixture to 4.5 with hydrochloric acid, and then use 10 % sodium hydroxide solution, and adjust its pH value to 7.5 to obtain a light yellow protein solution, which is spray-dried to obtain a finished product of decolorized hemoglobin powder.

Embodiment 2

[0026] Take 500kg of duck blood, centrifuge, take 100kg of blood cells, add 150kg of 0.5% salt solution, then stir the mixture under high-speed stirring for 1.5h, and then heat to 65°C. After taking it out, add 2000ml of defoamer to eliminate the bubbles in the mixture and prevent a large number of bubbles generated during oxidation. Immediately add 4000ml of 1.2g / ml calcium peroxide solution, adjust the pH of the mixture to 4.5 with hydrochloric acid, and then use 10% Sodium hydroxide solution, adjust its pH value to 7.5 to obtain a light yellow protein solution, which is spray-dried to obtain a finished product of decolorized hemoglobin powder.

Embodiment 3

[0028] Take 1000kg of chicken blood, centrifuge at 5000r / min, take 300kg of blood cells, add 600kg of 0.6% salt solution, then stir the mixture under high-speed stirring for 2.0h, and then heat to 70°C. After taking it out, add 6000ml of defoamer to eliminate the bubbles in the mixture and prevent a large number of bubbles generated during oxidation. Immediately add 6000ml of 1.5g / ml calcium peroxide solution, adjust the pH of the mixture to 3.5 with hydrochloric acid, and then use 10% Sodium hydroxide solution, the pH value is adjusted to 7.2 to obtain a light yellow protein solution, which is spray-dried to obtain a finished product of decolorized hemoglobin powder.

Technical field:

[0001] The invention discloses a blood cell decolorization method, which is applicable to a preparation method of decolorized blood globulin powder, and the method is suitable for companies that produce animal blood deep processing products on a large scale to decolorize blood cells in animal blood raw materials. Background technique:

[0002] Our country's animal husbandry is developed, and the slaughtering volume of live pigs accounts for 50% of the world's total, and a large amount of pig blood resources are produced every year. Pig blood is rich in nutrients, among which the protein content is as high as more than 18%, and the protein is rich in immunoglobulin, with a content of 22%. The amino acid content is rich and the ratio of various amino acids is relatively balanced. In addition, pig blood also contains various active substances such as trace elements, vitamins, and hormones. At this stage, the production of plasma protein powder and blood globul...

Examples

Embodiment 1

[0024] Take 30kg of pig blood, centrifuge, take 10kg of blood cell solution, add 10kg of 0.3% salt solution, then stir the mixed solution under high-speed stirring for 1.0h, and then heat to 65°C. After taking it out, add 200ml of defoamer to eliminate the bubbles in the mixture and prevent a large number of bubbles generated during oxidation, then immediately add 400ml of 1.5g / ml calcium peroxide solution, adjust the pH of the mixture to 4.5 with hydrochloric acid, and then use 10 % sodium hydroxide solution, and adjust its pH value to 7.5 to obtain a light yellow protein solution, which is spray-dried to obtain a finished product of decolorized hemoglobin powder.

Embodiment 2

[0026] Take 500kg of duck blood, centrifuge, take 100kg of blood cells, add 150kg of 0.5% salt solution, then stir the mixture under high-speed stirring for 1.5h, and then heat to 65°C. After taking it out, add 2000ml of defoamer to eliminate the bubbles in the mixture and prevent a large number of bubbles generated during oxidation. Immediately add 4000ml of 1.2g / ml calcium peroxide solution, adjust the pH of the mixture to 4.5 with hydrochloric acid, and then use 10% Sodium hydroxide solution, adjust its pH value to 7.5 to obtain a light yellow protein solution, which is spray-dried to obtain a finished product of decolorized hemoglobin powder.

Embodiment 3

[0028] Take 1000kg of chicken blood, centrifuge at 5000r / min, take 300kg of blood cells, add 600kg of 0.6% salt solution, then stir the mixture under high-speed stirring for 2.0h, and then heat to 70°C. After taking it out, add 6000ml of defoamer to eliminate the bubbles in the mixture and prevent a large number of bubbles generated during oxidation. Immediately add 6000ml of 1.5g / ml calcium peroxide solution, adjust the pH of the mixture to 3.5 with hydrochloric acid, and then use 10% Sodium hydroxide solution, the pH value is adjusted to 7.2 to obtain a light yellow protein solution, which is spray-dried to obtain a finished product of decolorized hemoglobin powder.