Method for extracting nutritional ingredients such as free amino acids from waste cordyceps militaris cocoons

Abstract

The invention discloses a method for extracting nutritional ingredients such as free amino acids from waste cordyceps militaris cocoons, aiming at extracting various nutritional substances keeping natural activity from the waste cordyceps militaris cocoons, thus increasing the utilization rate of cordyceps militaris and the cocoons, and supplying for production for different types of nutritional healthcare products. The method is characterized by adopting a sequential extraction process of freeze-thawing, combined enzymatic hydrolysis, and water extraction and alcohol precipitation, wherein generally, two of the following enzymes are used in a matching manner, and the hydrolysis temperatures of the enzymes are as follows respectively: the hydrolysis temperature of papain is 45+ / -2 DEG C; the hydrolysis temperatures of lysozyme, pectinase, amylase, pepsase and trypsin are 37+ / -2 DEG C; the hydrolysis temperatures of bromelain, glucoamylase, monascus and cellulose are 35+ / -2 DEG C; and the dosages of the enzymes are as follows respectively: in case of enzymatic hydrolysis for 1 kg of cordyceps militaris cocoon powder, 1 g of lysozyme for food, 2 g of amylase, 2 g of papain, 2 g of pepsase, 2 g of trypsin, 3 g of bromelain, 3 g of glucoamylase, 3 g of monascus, 3 g of cellulose and 4 g of pectinase are added.

Description

technical field

[0001] The invention relates to a method for extracting nutrient components such as free amino acids from waste cocoons of Cordyceps militaris. Background technique

[0002] At present, eating silkworm chrysalis is popular in many countries in the world; Americans use silkworm chrysalis to make sandwich chocolate, Australia generally uses silkworm chrysalis, and the French regard silkworm chrysalis as a high-protein delicacy, which is expensive. Many countries in Europe also eat silkworm chrysalis, so there are many kinds of silkworm chrysalis food on the market, such as: silkworm chrysalis biscuits, silkworm chrysalis milk, silkworm chrysalis wine, silkworm chrysalis canned food and so on.

[0003] Studies have shown that each kilogram of dried silkworm chrysalis contains about 60 grams of protein, 30 grams of fat, 1 gram of chitin, and 18 kinds of amino acids, 8 of which are necessary for the human body, and the proportion of the content is appropriate. ...

Examples

Embodiment Construction

[0125] According to the technological process of the present invention, after Cordyceps militaris and cocoons are mixed and crushed uniformly, the active ingredients are extracted through bioengineering enzyme treatment (separation, extraction, synthesis), and the main components are as follows: free amino acids, active polysaccharide cordycepin , protein, etc.

[0126] Clinical Trials and Results Analysis

[0127] 1. Toxicity test

[0128] 1. Acute toxicity test in mice:

[0129] Select 40 healthy mice weighing 18-22 g, half male and half male. Take Cordyceps militaris powder at a dose of 20g / kg.bw orally and gavage once on an empty stomach, observe for two weeks, and record the symptoms of poisoning and death.

[0130] Results During the 14-day observation period, no poisoning was observed, and none of the mice died. Show that this Cordyceps militaris cocoon powder is oral LD ​​to male and female mice 50 All greater than 10g / kg.bw. According to the acut...

technical field

[0001] The invention relates to a method for extracting nutrient components such as free amino acids from waste cocoons of Cordyceps militaris. Background technique

[0002] At present, eating silkworm chrysalis is popular in many countries in the world; Americans use silkworm chrysalis to make sandwich chocolate, Australia generally uses silkworm chrysalis, and the French regard silkworm chrysalis as a high-protein delicacy, which is expensive. Many countries in Europe also eat silkworm chrysalis, so there are many kinds of silkworm chrysalis food on the market, such as: silkworm chrysalis biscuits, silkworm chrysalis milk, silkworm chrysalis wine, silkworm chrysalis canned food and so on.

[0003] Studies have shown that each kilogram of dried silkworm chrysalis contains about 60 grams of protein, 30 grams of fat, 1 gram of chitin, and 18 kinds of amino acids, 8 of which are necessary for the human body, and the proportion of the content is appropriate. ...

Examples

Embodiment Construction

[0125] According to the technological process of the present invention, after Cordyceps militaris and cocoons are mixed and crushed uniformly, the active ingredients are extracted through bioengineering enzyme treatment (separation, extraction, synthesis), and the main components are as follows: free amino acids, active polysaccharide cordycepin , protein, etc.

[0126] Clinical Trials and Results Analysis

[0127] 1. Toxicity test

[0128] 1. Acute toxicity test in mice:

[0129] Select 40 healthy mice weighing 18-22 g, half male and half male. Take Cordyceps militaris powder at a dose of 20g / kg.bw orally and gavage once on an empty stomach, observe for two weeks, and record the symptoms of poisoning and death.

[0130] Results During the 14-day observation period, no poisoning was observed, and none of the mice died. Show that this Cordyceps militaris cocoon powder is oral LD ​​to male and female mice 50 All greater than 10g / kg.bw. According to the acut...

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