Method for inducing Chinese pholidota herb plant to regenerate and propagate by using pseudobulbs

Abstract

The invention belongs to a rapid propagation method for an important plant material in endangered wild plants and particularly relates to a method for inducing a Chinese pholidota herb plant to regenerate and propagate by using pseudobulbs. The method comprises the steps of material selection and sterilization; inducing culture; multiplication culture; inducing rooting; and transplanting. The method aims at solving the problems of seed germination difficulty in a natural state, poor natural propagation capacity, poor diffusion propagation capacity, natural regeneration difficulty, low division propagation speed and the like in the prior art, and provides the technical support for large-scale industrial production. By using the method, the propagation coefficient and the rooting rate are high, the plant growth time is short, the adaptability after culture is strong, a nursery stock is robust and straight, good in growth vigor, neat and consistent, the culture processes of the nursery stock are greatly shortened, and the cost is greatly reduced. The technical support is provided for protecting Chinese pholidota herbs and large-scale industrial production, popularization and application.

Description

technical field

[0001] The invention belongs to a method for rapid propagation of important plant materials in endangered wild plants, in particular to a method for inducing regeneration and propagation of shixiantao plants by using pseudobulbs. Background technique

[0002] Shixiantao ( Pholidota chinensis Lindl. ) is a plant of the orchidaceae genus Pholdota, also known as stone olive, stone peach and stone lotus. Perennial epiphytic herb, fleshy fleshy, bottle-shaped or ovate-shaped rhizome running horizontally, with two leaves, terminally grown on pseudobulbs, papery and tender. Shixiantao polysaccharide has good antioxidant effect. Especially anti-superoxide anion effect. The polysaccharide content in Shixiantao is not only high, but also has the functions of nourishing yin, clearing lung, promoting dampness, and eliminating blood stasis. Benefits stomach, relieves cough, fights cancer and improves immunity. There are about 30 species in the genus Stenophyllum, whi...

Examples

Embodiment 1

[0028] A technique for inducing the regeneration and propagation of shixiantao bulb plants, the specific steps are as follows

[0029] 1) Material selection and disinfection: 1. Select plump Shixiantao pseudobulbs as explants, pack them in fresh-keeping bags after picking, remove 2\3 leaves and impurities around the pseudobulbs after taking them back to the laboratory, and rinse them with detergent. Rinse in running water for 0.5h; 2. Plant disinfection treatment: Rinse the washed pseudobulb plants with double distilled water twice, put them in an ultra-clean workbench and sterilize them with 75% alcohol for 30 seconds, pour off the alcohol, add 0.1% mercury chloride (HgCl 2 ) for 8 minutes, pour the mercury solution into the waste mercury bottle, rinse with sterile water for 3 times, and then inoculate;

[0030] 2) Induction culture: Inoculate the sterilized materials with 1\3 leaf parts and the whole pseudobulb plant into the bulb bud induction medium sterilized by high tem...

technical field

[0001] The invention belongs to a method for rapid propagation of important plant materials in endangered wild plants, in particular to a method for inducing regeneration and propagation of shixiantao plants by using pseudobulbs. Background technique

[0002] Shixiantao ( Pholidota chinensis Lindl. ) is a plant of the orchidaceae genus Pholdota, also known as stone olive, stone peach and stone lotus. Perennial epiphytic herb, fleshy fleshy, bottle-shaped or ovate-shaped rhizome running horizontally, with two leaves, terminally grown on pseudobulbs, papery and tender. Shixiantao polysaccharide has good antioxidant effect. Especially anti-superoxide anion effect. The polysaccharide content in Shixiantao is not only high, but also has the functions of nourishing yin, clearing lung, promoting dampness, and eliminating blood stasis. Benefits stomach, relieves cough, fights cancer and improves immunity. There are about 30 species in the genus Stenophyllum, whi...

Examples

Embodiment 1

[0028] A technique for inducing the regeneration and propagation of shixiantao bulb plants, the specific steps are as follows

[0029] 1) Material selection and disinfection: 1. Select plump Shixiantao pseudobulbs as explants, pack them in fresh-keeping bags after picking, remove 2\3 leaves and impurities around the pseudobulbs after taking them back to the laboratory, and rinse them with detergent. Rinse in running water for 0.5h; 2. Plant disinfection treatment: Rinse the washed pseudobulb plants with double distilled water twice, put them in an ultra-clean workbench and sterilize them with 75% alcohol for 30 seconds, pour off the alcohol, add 0.1% mercury chloride (HgCl 2 ) for 8 minutes, pour the mercury solution into the waste mercury bottle, rinse with sterile water for 3 times, and then inoculate;

[0030] 2) Induction culture: Inoculate the sterilized materials with 1\3 leaf parts and the whole pseudobulb plant into the bulb bud induction medium sterilized by high tem...