The invention discloses an adhesive rubber water stop without isolation film, and a preparation method thereof. The adhesive rubber water stop without isolation film comprises a rubber water stop body, a self-adhesive glue layer and a concrete loving anti-adhesion coating, wherein the rubber water stop body, the self-adhesive glue layer and the concrete loving anti-adhesion coating are arranged insequence in a stacking manner. The self-adhesive glue layer comprises the following gradients, by mass part, of styrene-series thermoplastic elastomer compositions, tackifiers, plasticizers, antioxidants, ultraviolet light screening agents, couplers and white carbon black. The concrete loving anti-adhesion coating comprises the gradients, by mass part, of concrete loving lotion, filler, water, light stabilizers, dispersing agents, antifoaming agents, concrete loving couplers and titanium dioxide. The adhesive rubber water stop without isolation film is free of isolation film, is free of sandcovering operation, and is easier and more convenient in construction operation, and the construction efficiency is greatly improved; and at the same time, it can be avoided that the self-adhesive glue layer makes too much contact with the air, the situation that the self-adhesive glue layer is directly exposed to the blazing sun can be further avoided, the self-adhesive glue layer and the rubberwater stop are bonded more solid, and a better waterproof effect is achieved.