A kind of cleaning agent and its preparation method and application

Abstract

The invention discloses a preparation method of a cleaning agent, which contains a cleaning agent extract and water, wherein the preparation of the cleaning agent extract comprises the following steps: taking a seaweed extract, adding 0.3-1.0% of its mass of compound enzyme P to obtain Seaweed-compound enzyme P mixed solution; adjust the pH of the above mixed solution to 5-8, temperature to 25-50°C, and enzymatic hydrolysis reaction for 24-72 hours. The invention adopts a biological fermentation process to produce cleaning and antibacterial components, greatly improves the extraction efficiency of active components in the extract, and uses natural substances to avoid environmental pollution by being prepared as chemical cleaning agents. The method is simple and has wide applications.

Description

Technical field

[0001] The invention relates to the field of cleaning agents, in particular to a cleaning agent prepared by microbial fermentation and a preparation method and application thereof. Background technique

[0002] At present, there are many kinds of cleaning agents on the market, mainly chemical cleaning agents. The decontamination and cleaning ability of chemical cleaners mainly comes from surfactants. However, it is well known that chemical cleaners containing surfactants can cause double harm to the human body and the environment. On the one hand, surfactants can also penetrate into the human body. About 0.5% of the cleanser on the skin penetrates into the blood. If there is a wound on the skin, the penetration is increased by more than 10 times. The chemical detergent toxins that enter the human body can reduce the concentration of calcium ions in the blood, acidify the blood, and get tired easily. These toxins also reduce the liver's detox function. The tox...

Examples

Embodiment 1

[0045] Example 1: Beer fermentation tank cleaner

[0046] 1. Cleaner extract production process

[0047] 1. Preparation of seaweed extract

[0048] 1.1 Preparation of seaweed homogenate: fresh seaweed (Mixed by masses such as phylloxera, sargassum, brown seaweed, fucus, red palm-shaped algae, sea cysts, etc.): water according to mass ratio 1: (0.2 ~ 0.3) grinding, Prepare a homogenate, refrigerate at 3~6℃ for later use.

[0049] 1.2 Enzymatic hydrolysis ①: Add complex enzyme I according to the quality of the seaweed homogenate obtained above 0.2-0.5% (the complex enzyme I is a mixture of pectin hydrolase, proteolytic enzyme, and cellulase, and the dosage ratio is 1:1:( 2~3)), adjust pH 6~7, temperature 30~50℃, enzymolysis reaction 48~72h, get enzymolysis seaweed homogenate I.

[0050] 1.3 Fermentation①

[0051] 1.3.1 Cultivation of microbial agent I: Take a ring of slant bacteria, respectively inoculate Bacillus subtilis into liquid medium a, inoculate lactic acid bacteria into medium ...

Embodiment 2

[0094] Example 2: Cleaner for removing pesticide residues from fruits and vegetables

[0095] 1. Cleaning agent production process

[0096] 1. Preparation of seaweed extract

[0097] 1.1 Preparation of seaweed homogenate: fresh seaweed (Mixed by masses such as phylloxera, sargassum, brown seaweed, fucus, red palm-shaped algae, sea cysts, etc.): water according to mass ratio 1: (0.2 ~ 0.3) grinding, Prepare a homogenate, refrigerate at 3~6℃ for later use.

[0098] 1.2 Enzymatic hydrolysis ①: Add complex enzyme I according to the quality of the seaweed homogenate obtained above 0.2-0.5% (the complex enzyme I is a mixture of pectin hydrolase, proteolytic enzyme, and cellulase, and the dosage ratio is 1:1:( 2~3)), adjust pH 6~7, temperature 30~50℃, enzymolysis reaction 72h, get enzymolysis seaweed homogenate I.

[0099] 1.3 Fermentation①

[0100] 1.3.1 Cultivation of inoculum I: Take a ring of slant strains, respectively inoculate Bacillus subtilis in liquid medium a, inoculate lactic acid...

Embodiment 3

[0120] Example 3: Sterilization hand sanitizer

[0121] 1. Cleaner extract production process

[0122] 1. Preparation of seaweed extract

[0123] 1.1 Preparation of seaweed homogenate: fresh seaweed (Mixed by masses such as phylloxera, sargassum, brown seaweed, fucus, red palm-shaped algae, sea cysts, etc.): water according to mass ratio 1: (0.2 ~ 0.3) grinding, Prepare a homogenate, refrigerate at 3~6℃ for later use.

[0124] 1.2 Enzymatic hydrolysis ①: Add complex enzyme I according to the quality of the seaweed homogenate obtained above 0.2-0.5% (the complex enzyme I is a mixture of pectin hydrolase, proteolytic enzyme, and cellulase, and the dosage ratio is 1:1:( 2~3)), adjust pH 6~7, temperature 30~50℃, enzymolysis reaction 72h, get enzymolysis seaweed homogenate I.

[0125] 1.3 Fermentation①

[0126] 1.3.1 Cultivation of inoculum I: Take a ring of slant strains, respectively inoculate Bacillus subtilis in liquid medium a, inoculate lactic acid bacteria into liquid medium b, inocu...

Technical field

[0001] The invention relates to the field of cleaning agents, in particular to a cleaning agent prepared by microbial fermentation and a preparation method and application thereof. Background technique

[0002] At present, there are many kinds of cleaning agents on the market, mainly chemical cleaning agents. The decontamination and cleaning ability of chemical cleaners mainly comes from surfactants. However, it is well known that chemical cleaners containing surfactants can cause double harm to the human body and the environment. On the one hand, surfactants can also penetrate into the human body. About 0.5% of the cleanser on the skin penetrates into the blood. If there is a wound on the skin, the penetration is increased by more than 10 times. The chemical detergent toxins that enter the human body can reduce the concentration of calcium ions in the blood, acidify the blood, and get tired easily. These toxins also reduce the liver's detox function. The tox...

Examples

Embodiment 1

[0045] Example 1: Beer fermentation tank cleaner

[0046] 1. Cleaner extract production process

[0047] 1. Preparation of seaweed extract

[0048] 1.1 Preparation of seaweed homogenate: fresh seaweed (Mixed by masses such as phylloxera, sargassum, brown seaweed, fucus, red palm-shaped algae, sea cysts, etc.): water according to mass ratio 1: (0.2 ~ 0.3) grinding, Prepare a homogenate, refrigerate at 3~6℃ for later use.

[0049] 1.2 Enzymatic hydrolysis ①: Add complex enzyme I according to the quality of the seaweed homogenate obtained above 0.2-0.5% (the complex enzyme I is a mixture of pectin hydrolase, proteolytic enzyme, and cellulase, and the dosage ratio is 1:1:( 2~3)), adjust pH 6~7, temperature 30~50℃, enzymolysis reaction 48~72h, get enzymolysis seaweed homogenate I.

[0050] 1.3 Fermentation①

[0051] 1.3.1 Cultivation of microbial agent I: Take a ring of slant bacteria, respectively inoculate Bacillus subtilis into liquid medium a, inoculate lactic acid bacteria into medium ...

Embodiment 2

[0094] Example 2: Cleaner for removing pesticide residues from fruits and vegetables

[0095] 1. Cleaning agent production process

[0096] 1. Preparation of seaweed extract

[0097] 1.1 Preparation of seaweed homogenate: fresh seaweed (Mixed by masses such as phylloxera, sargassum, brown seaweed, fucus, red palm-shaped algae, sea cysts, etc.): water according to mass ratio 1: (0.2 ~ 0.3) grinding, Prepare a homogenate, refrigerate at 3~6℃ for later use.

[0098] 1.2 Enzymatic hydrolysis ①: Add complex enzyme I according to the quality of the seaweed homogenate obtained above 0.2-0.5% (the complex enzyme I is a mixture of pectin hydrolase, proteolytic enzyme, and cellulase, and the dosage ratio is 1:1:( 2~3)), adjust pH 6~7, temperature 30~50℃, enzymolysis reaction 72h, get enzymolysis seaweed homogenate I.

[0099] 1.3 Fermentation①

[0100] 1.3.1 Cultivation of inoculum I: Take a ring of slant strains, respectively inoculate Bacillus subtilis in liquid medium a, inoculate lactic acid...

Embodiment 3

[0120] Example 3: Sterilization hand sanitizer

[0121] 1. Cleaner extract production process

[0122] 1. Preparation of seaweed extract

[0123] 1.1 Preparation of seaweed homogenate: fresh seaweed (Mixed by masses such as phylloxera, sargassum, brown seaweed, fucus, red palm-shaped algae, sea cysts, etc.): water according to mass ratio 1: (0.2 ~ 0.3) grinding, Prepare a homogenate, refrigerate at 3~6℃ for later use.

[0124] 1.2 Enzymatic hydrolysis ①: Add complex enzyme I according to the quality of the seaweed homogenate obtained above 0.2-0.5% (the complex enzyme I is a mixture of pectin hydrolase, proteolytic enzyme, and cellulase, and the dosage ratio is 1:1:( 2~3)), adjust pH 6~7, temperature 30~50℃, enzymolysis reaction 72h, get enzymolysis seaweed homogenate I.

[0125] 1.3 Fermentation①

[0126] 1.3.1 Cultivation of inoculum I: Take a ring of slant strains, respectively inoculate Bacillus subtilis in liquid medium a, inoculate lactic acid bacteria into liquid medium b, inocu...

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