Method for detecting Yinqiao detoxification mixture

Abstract

The invention relates to a method for detecting a Yinqiao detoxification mixture. The method comprises trait, component identification and inspection items, wherein the component identification comprises thin layer chromatography identification of flos genkwa, thin layer chromatography identification of flos lonicerae, thin layer chromatography identification of herba menthae, thin layer chromatography identification of fructus forsythiae and thin layer chromatography identification of fructus arctii and licorice root. The method is carried out aiming at the problem that a conventional detection method cannot conduct corresponding monitoring on effective components and cannot detect the mixing of the fake flos genkwa, the scientific, reasonable and feasible component identification methodis formulated, a low-toxic and non-toxic reagent is adopted to replace trichloromethane, environmental pollution is reduced, damage to the health of inspectors is reduced, clinical effects of drugs are ensured, and the fake flos genkwa in the flos lonicerae can also be effectively detected to avoid toxic and harmful components which endanger the health of patients.

Description

Technical field

[0001] The invention relates to a method for detecting a mixture of traditional Chinese medicine, in particular to a method for detecting a mixture of Yinqiaojiedu. Background technique

[0002] Yinqiao Jiedu Mixture is a variety included in the 14th volume of Chinese Medicine Prescription Preparations of the Ministry of Health Drug Standards. The standard number is WS3-B-2783-97. The prescription is honeysuckle, forsythia, peppermint, nepeta, light tempeh, Burdock seed, Platycodon grandiflorum, pale bamboo leaves, licorice, is a mixture of pure Chinese medicine, which has the effects of pungent cooling and relieving surface, clearing heat and detoxification. It is currently clinically used for wind-heat and cold, fever, headache, cough, dry mouth, and sore throat.

[0003] The testing standards for Yinqiao Jiedu Mixture were formulated in the 1990s and have historical limitations: on the one hand, chloroform, namely chloroform, and n-hexane are used in the testing ...

Examples

Embodiment 1

[0027] [Prescription] Honeysuckle 180g Forsythia 180g Peppermint 108g Nepeta 72g Light tempeh 90g Burdock seed (fried) 108g Platycodon grandiflorum 108g Light bamboo leaf 72g Licorice 90g

[0028] [Preparation method] The above nine flavors, light tempeh, boiled in water, warm soak at 80℃ twice for 2 hours each time, combine the infusions, and filter; mint and nepeta are distilled to extract the volatile oil, and the distilled aqueous solution is separated Collect; decocting the medicine dregs and other six flavors such as honeysuckle with water twice for 2 hours each time, combine the decoction, filter, and combine the filtrate with the above-mentioned medicines and concentrate to about 1000ml, add appropriate preservatives, let cool, and add volatile oil , Add water to 1000ml, stir evenly, and get it.

[0029] [Properties] This product is light brown to brown liquid; it has a fragrant air and slightly bitter taste.

[0030] [Component identification] (1) The component identificati...

Embodiment 2

[0039] Except that the component identification is different from Example 1, the other methods are the same.

[0040] [Component identification] (1) The component identification of Daphne genkwa is: take 20ml of this product, add 40ml of petroleum ether at 60~90℃, shake and extract twice, 20ml each time, combine the extracts, evaporate until nearly dry, add methanol 1ml, as the test solution, another reference substance of daikon, add methanol to make a solution containing 1mg per 1ml, as the reference solution, according to thin-layer chromatography test, draw 5μl each of the above solutions, respectively on the same silica gel G thin-layer plate, toluene: ethyl acetate: formic acid = 10: 6: 0.3 by volume ratio as the developing agent, unfold, take out to dry, and inspect under 365nm ultraviolet light. There are no fluorescent spots of the same color on the corresponding position of the product color spectrum;

[0041] (2) The composition of honeysuckle is: take 10ml of this prod...

Embodiment 3

[0046] Except that the component identification is different from Example 1, the other methods are the same.

[0047] [Component identification] (1) The component identification of Daphne genkwa is: take 20ml of this product, add 40ml of petroleum ether at 60~90℃, shake and extract twice, 20ml each time, combine the extracts, evaporate until nearly dry, add methanol 1ml, as the test solution, another reference substance of daikon, add methanol to make a solution containing 1mg per 1ml, as the reference solution, according to thin-layer chromatography test, draw 5μl each of the above solutions, respectively on the same silica gel G thin layer plate, toluene: ethyl acetate: formic acid = 8: 4: 0.2 by volume as the developing agent, unfold, take out to dry, and inspect under a 365nm ultraviolet light. There are no fluorescent spots of the same color on the corresponding position of the product color spectrum;

[0048] (2) The composition of honeysuckle is: take 10ml of this product, ...

Technical field

[0001] The invention relates to a method for detecting a mixture of traditional Chinese medicine, in particular to a method for detecting a mixture of Yinqiaojiedu. Background technique

[0002] Yinqiao Jiedu Mixture is a variety included in the 14th volume of Chinese Medicine Prescription Preparations of the Ministry of Health Drug Standards. The standard number is WS3-B-2783-97. The prescription is honeysuckle, forsythia, peppermint, nepeta, light tempeh, Burdock seed, Platycodon grandiflorum, pale bamboo leaves, licorice, is a mixture of pure Chinese medicine, which has the effects of pungent cooling and relieving surface, clearing heat and detoxification. It is currently clinically used for wind-heat and cold, fever, headache, cough, dry mouth, and sore throat.

[0003] The testing standards for Yinqiao Jiedu Mixture were formulated in the 1990s and have historical limitations: on the one hand, chloroform, namely chloroform, and n-hexane are used in the testing ...

Examples

Embodiment 1

[0027] [Prescription] Honeysuckle 180g Forsythia 180g Peppermint 108g Nepeta 72g Light tempeh 90g Burdock seed (fried) 108g Platycodon grandiflorum 108g Light bamboo leaf 72g Licorice 90g

[0028] [Preparation method] The above nine flavors, light tempeh, boiled in water, warm soak at 80℃ twice for 2 hours each time, combine the infusions, and filter; mint and nepeta are distilled to extract the volatile oil, and the distilled aqueous solution is separated Collect; decocting the medicine dregs and other six flavors such as honeysuckle with water twice for 2 hours each time, combine the decoction, filter, and combine the filtrate with the above-mentioned medicines and concentrate to about 1000ml, add appropriate preservatives, let cool, and add volatile oil , Add water to 1000ml, stir evenly, and get it.

[0029] [Properties] This product is light brown to brown liquid; it has a fragrant air and slightly bitter taste.

[0030] [Component identification] (1) The component identificati...

Embodiment 2

[0039] Except that the component identification is different from Example 1, the other methods are the same.

[0040] [Component identification] (1) The component identification of Daphne genkwa is: take 20ml of this product, add 40ml of petroleum ether at 60~90℃, shake and extract twice, 20ml each time, combine the extracts, evaporate until nearly dry, add methanol 1ml, as the test solution, another reference substance of daikon, add methanol to make a solution containing 1mg per 1ml, as the reference solution, according to thin-layer chromatography test, draw 5μl each of the above solutions, respectively on the same silica gel G thin-layer plate, toluene: ethyl acetate: formic acid = 10: 6: 0.3 by volume ratio as the developing agent, unfold, take out to dry, and inspect under 365nm ultraviolet light. There are no fluorescent spots of the same color on the corresponding position of the product color spectrum;

[0041] (2) The composition of honeysuckle is: take 10ml of this prod...

Embodiment 3

[0046] Except that the component identification is different from Example 1, the other methods are the same.

[0047] [Component identification] (1) The component identification of Daphne genkwa is: take 20ml of this product, add 40ml of petroleum ether at 60~90℃, shake and extract twice, 20ml each time, combine the extracts, evaporate until nearly dry, add methanol 1ml, as the test solution, another reference substance of daikon, add methanol to make a solution containing 1mg per 1ml, as the reference solution, according to thin-layer chromatography test, draw 5μl each of the above solutions, respectively on the same silica gel G thin layer plate, toluene: ethyl acetate: formic acid = 8: 4: 0.2 by volume as the developing agent, unfold, take out to dry, and inspect under a 365nm ultraviolet light. There are no fluorescent spots of the same color on the corresponding position of the product color spectrum;

[0048] (2) The composition of honeysuckle is: take 10ml of this product, ...