Method for removing Raman baseline drift in Raman spectrum

A Raman spectrum and Raman technology, applied in Raman scattering, material excitation analysis, instruments, etc., can solve the problem of not being able to remove the fluorescent package well, and achieve the effect of speeding up and ensuring stability

CN108918499BActive Publication Date: 2019-08-02SOUTH CHINA NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2019-08-02

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Abstract

The invention discloses a method for eliminating drifting of a Raman baseline in a Raman spectrum. The method comprises the following steps: step I: smoothening an original Raman spectrum, solving a Raman peak after the smoothening according to a middle value greater than values on two sides, solving a valley after the smoothening according to the middle value smaller than values on two sides, andobtaining a smoothened Raman spectrum; step II: performing the sliding-window linear fitting for a smoothened Raman area, and obtaining a fit Raman baseline; step III: eliminating a fluorescent packet in the fit Raman baseline; and step IV: subtracting the Raman baseline with the fluorescent packet being removed obtained in step III from the original Raman peak spectrum, thus obtaining the finalRaman spectrum with the drifting of the Raman baseline being eliminated. By adopting the method, the stability in the correction process can be ensured, and the correction speed of the baseline can increase.
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Description

technical field

[0001] The invention relates to a processing method for Raman baseline drift in the optical field, in particular to a method for removing Raman baseline drift in a Raman spectrum. Background technique

[0002] Raman spectroscopy plays an important role in the field of biological analysis and detection. In recent years, Raman spectroscopy has been used in the classification and analysis of cells. This often requires more accurate data for algorithm analysis. In Raman detection, background fluorescence is the most common background interference light. The appearance of background fluorescence is usually much wider than the Raman peak, which looks like a slowly changing baseline of the Raman spectrum. Background fluorescence can cause a shift in the Raman baseline, resulting in inaccurate Raman data.

[0003] Common baseline correction methods include derivation, polynomial fitting, wavelet transform, etc. The Savitzky-Golay derivative spectrum is a commonly...

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Embodiment Construction

[0029] The method for removing Raman baseline drift in the Raman spectrum of the present invention comprises the steps:

[0030] The first step: smooth the original Raman spectrum, obtain the smoothed Raman peak by the middle value being larger than the two sides, and obtain the smoothed trough by making the middle value smaller than the two sides, and obtain the smoothed Raman Atlas;

[0031] Step 2: Perform sliding window linear fitting on the smoothed Raman region to obtain the fitted Raman baseline;

[0032] Select the trough whose abscissa is X in the smoothed Raman spectrum, the abscissa of the next trough is X1, the abscissa of the next trough is X2, and the abscissa of the next trough is X3, and the trough whose abscissa is X is obtained The cosine value of the angle between the straight line connected to the trough whose abscissa is X1 and the negative semi-axis of the y-axis is recorded as a1, and the straight line connected to the trough whose abscissa is X and the...