Cell smear detection method

Abstract

The invention discloses a cell smear detection method. The cell smear detection method comprises the following steps: S1: preparing materials: pumping a proper amount of midstream urine in the morning; injecting the urine into a container; then injecting a proper amount of an anticoagulant into the container, so as to obtain a mixed solution; S2: carrying out centrifugal treatment: adding the mixed solution into a centrifugal machine and carrying out centrifugal operation; after the centrifugal separation is finished, removing supernatant to obtain sediment; S3: manufacturing a specimen: sucking the sediment through a suction pipe; then injecting the sediment sucked by the suction pipe into a slide; then covering a cover glass on the slide; pulling the cover glass in the opposite directionto manufacture the specimen; and S4: fixing the specimen: dropwise adding a proper amount of an ethanol solution onto the manufactured specimen. According to the cell smear detection method disclosedby the invention, the shape of cells in the urine can be effectively maintained and the cells in the urine is more uniformly distributed, so that the cells in the urine are easy to classify and the accuracy of cytological detection in the urine is greatly improved; and a smear is uniform and the colored cells are bright and uniform and have a clear structure.

Description

technical field

[0001] The invention relates to the technical field of cell smears, in particular to a cell smear detection method. Background technique

[0002] With the continuous development of science and the interpenetration among disciplines, the field of hematology has been continuously expanded, and the detection methods have become more and more abundant. Cell biochemistry, immunology, genetics, cell dynamics, and electron microscopy have brought morphological examinations to the molecular level, and in daily clinical work, cell morphological examinations under light microscopy are the preferred and essential means. Urine examination can be used for some infectious diseases. The current smear cells are unevenly distributed and accumulated too much. Mucus, blood or inflammatory cells cover abnormal cells. The cells are often distributed in multiple layers. Abnormal cells often overlap, which affects observation. Therefore, we propose a cell smear detection method to...

Examples

Embodiment

[0022] A cell smear detection method is proposed in this example, which includes the following steps: S1: Material preparation: extract an appropriate amount of urine in the middle of the morning, inject the urine into a container, and then inject an appropriate amount of anticoagulant into the container to obtain Mixed solution; S2: Centrifugation: Add the mixed solution to the centrifuge and perform centrifugation. After the centrifugation is completed, remove the supernatant to obtain the sediment; S3: Specimen preparation: suck the sediment through the straw, and then suck it up with the straw Inject the precipitate into the slide glass, then cover the slide glass with a cover glass, and pull the cover glass in the opposite direction to prepare the specimen; S4: Specimen fixation: drop an appropriate amount of ethanol solution on the prepared specimen , to fix the specimen; S5: microscope observation: put the fixed specimen under the microscope for observation and treatment...

technical field

[0001] The invention relates to the technical field of cell smears, in particular to a cell smear detection method. Background technique

[0002] With the continuous development of science and the interpenetration among disciplines, the field of hematology has been continuously expanded, and the detection methods have become more and more abundant. Cell biochemistry, immunology, genetics, cell dynamics, and electron microscopy have brought morphological examinations to the molecular level, and in daily clinical work, cell morphological examinations under light microscopy are the preferred and essential means. Urine examination can be used for some infectious diseases. The current smear cells are unevenly distributed and accumulated too much. Mucus, blood or inflammatory cells cover abnormal cells. The cells are often distributed in multiple layers. Abnormal cells often overlap, which affects observation. Therefore, we propose a cell smear detection method to...

Examples

Embodiment

[0022] A cell smear detection method is proposed in this example, which includes the following steps: S1: Material preparation: extract an appropriate amount of urine in the middle of the morning, inject the urine into a container, and then inject an appropriate amount of anticoagulant into the container to obtain Mixed solution; S2: Centrifugation: Add the mixed solution to the centrifuge and perform centrifugation. After the centrifugation is completed, remove the supernatant to obtain the sediment; S3: Specimen preparation: suck the sediment through the straw, and then suck it up with the straw Inject the precipitate into the slide glass, then cover the slide glass with a cover glass, and pull the cover glass in the opposite direction to prepare the specimen; S4: Specimen fixation: drop an appropriate amount of ethanol solution on the prepared specimen , to fix the specimen; S5: microscope observation: put the fixed specimen under the microscope for observation and treatment...