Washing device for chemiluminescent immunoassay analyzer

Abstract

The present invention relates to the technical field of manufacturing chemiluminescent immunoassay analyzers, and in particular to a washing device for a chemiluminescent immunoassay optical analyzer, which can effectively improve the washing effect without increasing the number of washing times. The device is characterized in that the magnetic bead adsorption mechanism is provided with a magnet mounting plate, and more than two magnet mounting grooves are provided on the magnet mounting plate, and two magnets are arranged side by side in each magnet mounting groove, and the magnetic pole directions of the two magnets in the same magnet mounting groove are reversed, and the magnetic attraction directions of the two magnets at the same height in the adjacent magnet mounting grooves are also reversed; a pre-adsorption magnet mounting groove is further provided on the inner side wall of the magnet mounting plate or the rotating disk at a position corresponding to the starting position of the adsorption process, and a magnet is installed in the pre-adsorption magnet mounting groove.

Description

Technical field:

[0001] The invention relates to the technical field of manufacturing chemiluminescent immunoassay analyzers, in particular to a washing device for chemiluminescent immunoassay analyzers which can effectively improve the washing effect without increasing the number of washing times. Background technology:

[0002] A fully automatic chemiluminescence immunoassay analyzer is an ultra-micro analysis device established by combining immune reaction with luminescence analysis. For example, patent document CN103599898 discloses a fully automatic chemiluminescence immunoassay cleaning device, which provides a cleaning structure for assembly-line cleaning of sample magnetic beads. However, in actual use, we found that such existing products have the following defects: (1) The lower needle position of the cleaning needle is located in the center of the reaction cup, so a small amount of magnetic beads carrying effective substances will be sucked away when the waste liquid is sucked away, causing the loss of magnetic beads and the risk of cross-contamination; (2) The magnetic bead adsorption device adopted by it adopts a single magnet structure with weak suction force, so the magnetic beads are slowly adsorbed. The single magnet structure causes the magnetic beads to be adsorbed in the form of points or strips, so the magnetic beads are easily formed into a multi-layer adsorption structure, resulting in impurities in adjacent layers that cannot be removed. Summary of the invention:

[0003] In view of the shortcomings and deficiencies in the prior art, the present invention proposes a washing device for a chemical immunoassay optical analyzer which can effectively improve the washing effect without increasing the number of washing times.

[0004] The present invention can be achieved by the following measures:

[0005] A washing device for a chemical immunoassay optical analyzer is provided with a magnetic bead adsorption mechanism, characterized in that the magnetic bead adsorption mechanism is provided with a magnet mounting plate, on which more than two magnet mounting slots are provided, in each magnet mounting slot two magnets are arranged side by side up and down, the magnetic pole directions of the two magnets in the same magnet mounting slot are reversed, and the magnetic attraction directions of the two magnets at the same height in adjacent magnet mounting slots are also reversed; a pre-adsorption magnet mounting slot is also provided on the inner side wall of the magnet mounting plate or the rotating disk at a position corresponding to the starting position of the adsorption process, and a magnet is installed in the pre-adsorption magnet mounting slot.

[0006] The present invention arranges two pre-adsorption magnet installation grooves in parallel at a position corresponding to the starting position of the adsorption treatment on the magnet installation plate along the rotation direction of the reaction cup rotating disk. A magnet is arranged in each pre-adsorption magnet installation groove, which is used to perform pre-adsorption treatment on the magnetic beads in the reaction cup before the washing process starts, so as to improve the adsorption and cleaning effect.

[0007] The invention is also provided with a reaction cup rotating disk and a lifting needle stand. A channel for passing the sample is provided on the reaction cup rotating disk. The magnetic bead adsorption mechanism is arranged on the side of the channel, and the lifting support is arranged above the channel.

[0008] The lifting needle rack of the present invention is provided with at least two groups of injection and aspiration combination needles, and the injection and aspiration combination needles are composed of a aspiration needle and an injection needle, wherein the needle tip position of the lower end of the aspiration needle is lower than the needle tip position of the lower end of the injection needle, and the needle tip of the injection needle is bent in the direction close to the aspiration needle, thereby ensuring that when absorbing waste liquid, the needle tip of the aspiration needle can reach the bottom of the sample in the reaction cup and fully absorb impurities, and when injecting cleaning liquid, the needle tip of the injection needle is located above the needle tip of the aspiration needle, and the cleaning liquid injected by the injection needle can rinse the aspiration needle to avoid contamination of different samples.

[0009] The aspiration needle and injection needle of the present invention are both arranged on a side above the reaction cup away from the magnet adsorption mechanism via a lifting bracket, thereby ensuring that the aspiration needle descends to the opposite side of the reaction cup where the magnetic beads are adsorbed and aggregated during aspiration, thereby preventing the magnetic beads from being lost.

[0010] The magnet mounting plate described in the present invention is composed of a vertical plate and a bottom plate, which are vertically fixedly connected and have an L-shaped cross-section. The side of the vertical plate is provided with more than two U-shaped grooves for placing magnets, and the opening direction of the U-shaped grooves faces upward. The top of the vertical plate is provided with a pressing plate for closing the openings of the more than two U-shaped grooves, and the pressing plate is fixedly connected to the top of the vertical plate by screws. The bottom plate is provided with a splicing tooth for engaging with the rotating chassis; two magnets placed side by side up and down are arranged in the U-shaped groove on the side of the vertical plate, and a rust-proof iron plate is arranged on the outer side of the magnet to enhance the magnetism. Furthermore, the outer side of the rust-proof iron plate is covered with a shading tape to improve the light-shielding property of the entire mechanism; the vertical plate and the bottom plate are both in an arc shape adapted to the inner side of the rotating disk body, a reaction cup carrying track is provided on the rotating disk, and an installation notch that passes through the reaction cup carrying track for assembling the magnet mounting plate is provided at a position corresponding to the washing position on the inner wall, and the installation notch is provided with an installation groove that matches the splicing tooth.

[0011] During the use of the present invention, the reaction cup rotating disk drives more than two reaction cups containing samples to be tested to move forward in a set direction. The driving mechanism for driving the reaction cup rotating disk to rotate can drive the reaction cup rotating disk to move forward one reaction cup position each time. When the washing process starts, the reaction cup first enters the pre-adsorption processing area under the drive of the rotating disk, and the single-grain magnet in the pre-adsorption magnet installation groove on the magnet installation plate performs pre-adsorption processing on the magnetic beads in the reaction cup, so that the magnetic beads originally scattered in the entire space of the reaction cup are close to the side where the magnetic bead adsorption mechanism is located. When the rotating disk drives the reaction cup to enter the double-grain magnet adsorption area, the magnetic beads are quickly adsorbed to the cup wall of the reaction cup close to the magnet. At this time, due to the use of double-grain magnet adsorption, the magnetic beads in the reaction cup are flattened on the cup wall of the reaction cup, and the agglomeration phenomenon is effectively reduced. The cleaning effect is prevented from being affected by impurities wrapped by multi-layer agglomerated magnetic beads; at the same time, the lifting needle rack is lifted and lowered by the drive of the needle rack driving mechanism to complete the injection of cleaning liquid and the absorption of waste liquid, wherein the position of the needle tip at the lower end of the aspiration needle is lower than the position of the needle tip at the lower end of the injection needle, and the needle tip of the injection needle is bent toward the direction close to the aspiration needle, thereby ensuring that when absorbing waste liquid, the needle tip of the aspiration needle can reach the bottom of the sample in the reaction cup and fully absorb impurities, and when injecting cleaning liquid, the needle tip of the injection needle is located above the needle tip of the aspiration needle, and the cleaning liquid injected by the injection needle can rinse the aspiration needle to avoid contamination of different samples; in addition, the aspiration needle and the injection needle are both arranged on the side of the reaction cup deviating from the magnet adsorption mechanism above the reaction cup via the lifting bracket, thereby ensuring that the aspiration needle descends to the opposite side of the reaction cup where the magnetic beads are adsorbed and agglomerated when absorbing liquid, thereby avoiding the loss of magnetic beads.

[0012] Compared with the prior art, the present invention can effectively improve the cleaning effect and reduce the loss of magnetic beads without increasing the number of cleaning times, and has significant advantages such as reasonable structure, simple operation, and reliable operation. Description of the drawings:

[0013] Attached Figure 1 It is a structural schematic diagram of the present invention.

[0014] Attached Figure 2 It is a schematic diagram of the structure of the combined injection and aspiration needle of the present invention.

[0015] Attached Figure 3 yes Figure 2 Cross-section view in the AA direction.

[0016] Attached Figure 4 It is a structural schematic diagram of the magnet mounting plate in the present invention.

[0017] Figure numerals: rotating disk 1, lifting needle rack 2, magnet 3, pre-adsorption magnet mounting groove 4, magnet mounting plate 5, liquid injection and liquid aspiration combination needle 6, aspiration needle 7, liquid injection needle 8, vertical plate 9, bottom plate 10, magnetic bead adsorption surface 11, U-shaped groove 12, pressure plate 13, and splicing teeth 14. Specific implementation method:

[0018] The present invention will be further described below in conjunction with the accompanying drawings.

[0019] As attached Figure 1 As shown, the present invention proposes a washing device for a chemical immunoassay optical analyzer, which is provided with a reaction cup rotating disk 1, a lifting needle holder 2, and a magnetic bead adsorption mechanism, characterized in that the magnetic bead adsorption mechanism is provided with a magnet mounting plate 5, on which more than two magnet mounting grooves are provided, and in each magnet mounting groove, two magnets 3 are arranged side by side up and down, the magnetic pole directions of the two magnets 3 located in the same magnet mounting groove are reversed, and the magnetic attraction directions of the two magnets 3 located at the same height in adjacent magnet mounting grooves are also reversed; a pre-adsorption magnet mounting groove 4 is also provided on the inner side wall of the magnet mounting plate 5 or the rotating disk 1 at a position corresponding to the starting position of the adsorption process, and a magnet is installed in the pre-adsorption magnet mounting groove 4.

[0020] In the present invention, two pre-adsorption magnet mounting grooves 4 are arranged in parallel at a position corresponding to the starting position of the adsorption treatment on the magnet mounting plate 5 and along the rotation direction of the reaction cup rotating disk. A magnet is arranged in each pre-adsorption magnet mounting groove 4, which is used to perform pre-adsorption treatment on the magnetic beads in the reaction cup before the washing process starts, so as to improve the adsorption and cleaning effect.

[0021] As attached Figure 2 As shown, the lifting needle rack 2 of the present invention is provided with at least two groups of injection and aspiration combination needles 6, and the injection and aspiration combination needle 6 is composed of a aspiration needle 7 and an injection needle 8, wherein the needle tip position of the lower end of the aspiration needle 7 is lower than the needle tip position of the lower end of the injection needle 8, and the needle tip of the injection needle 8 is bent toward the direction close to the aspiration needle 7, so as to ensure that when absorbing waste liquid, the needle tip of the aspiration needle 7 can reach the bottom of the sample in the reaction cup 9 and fully absorb impurities, and when injecting cleaning liquid, the needle tip of the injection needle 8 is located above the needle tip of the aspiration needle 7, and the cleaning liquid injected by the injection needle 8 can rinse the needle tip of the aspiration needle 7 to avoid contamination of different samples.

[0022] As attached Figure 3 As shown, the aspiration needle 7 and the injection needle of the present invention are both arranged on the side of the reaction cup away from the magnetic adsorption mechanism via the lifting bracket 2, thereby ensuring that the aspiration needle 7 descends to the opposite side of the reaction cup where the magnetic beads are adsorbed and aggregated when aspirating liquid, thereby avoiding the loss of magnetic beads.

[0023] As attached Figure 4As shown, the magnet mounting plate 5 of the present invention is composed of a vertical plate 9 and a bottom plate 10, the vertical plate 9 and the bottom plate 10 are vertically fixedly connected, and the cross section is L-shaped, the side of the vertical plate 9 is provided with more than two U-shaped grooves 12 for placing magnets, the opening direction of the U-shaped groove 12 is upward, the top of the vertical plate 9 is provided with a pressing plate 13 for closing the openings of the more than two U-shaped grooves 12, the pressing plate 13 is fixedly connected to the top of the vertical plate 9 by screws, and the bottom plate 10 is provided with a splicing tooth 14 for engaging with the rotating disk 1; the U-shaped groove on the side of the vertical plate 9 is provided with upper and lower side-by-side placed Two magnets are provided, and a rust-proof iron plate is arranged on the outer side of the magnet to strengthen the magnetism. Furthermore, a light-shielding tape is pasted on the outer side of the rust-proof iron plate to improve the light-shielding property of the whole mechanism. Both the vertical plate 9 and the bottom plate 10 are arc-shaped to adapt to the inner side of the main body of the rotating disk 1, and the vertical plate 9 and the bottom plate 10 are an integrated structure. A reaction cup carrying track is provided on the rotating disk 1, and an installation notch which is used for assembling the magnet mounting plate and passes through the reaction cup carrying track is provided at a position corresponding to the washing position on the inner wall, and an installation groove which cooperates with the splicing tooth 14 is provided on the installation notch.

[0024] During the use of the present invention, the reaction cup rotating disk drives more than two reaction cups containing samples to be tested to move forward in a set direction. The driving mechanism for driving the reaction cup rotating disk to rotate can drive the reaction cup rotating disk to move forward one reaction cup position each time. When the washing process starts, the reaction cup first enters the pre-adsorption processing area under the drive of the rotating disk, and the single-grain magnet in the pre-adsorption magnet installation groove on the magnet installation plate performs pre-adsorption processing on the magnetic beads in the reaction cup, so that the magnetic beads originally scattered in the entire space of the reaction cup are close to the side where the magnetic bead adsorption mechanism is located. When the rotating disk drives the reaction cup to enter the double-grain magnet adsorption area, the magnetic beads are quickly adsorbed to the cup wall of the reaction cup close to the magnet. At this time, due to the use of double-grain magnet adsorption, the magnetic beads in the reaction cup are flattened on the cup wall of the reaction cup, and the agglomeration phenomenon is effectively reduced. The cleaning effect is prevented from being affected by impurities wrapped by multi-layer agglomerated magnetic beads; at the same time, the lifting needle rack is lifted and lowered by the drive of the needle rack driving mechanism to complete the injection of cleaning liquid and the absorption of waste liquid, wherein the position of the needle tip at the lower end of the aspiration needle is lower than the position of the needle tip at the lower end of the injection needle, and the needle tip of the injection needle is bent toward the direction close to the aspiration needle, thereby ensuring that when absorbing waste liquid, the needle tip of the aspiration needle can reach the bottom of the sample in the reaction cup and fully absorb impurities, and when injecting cleaning liquid, the needle tip of the injection needle is located above the needle tip of the aspiration needle, and the cleaning liquid injected by the injection needle can rinse the aspiration needle to avoid contamination of different samples; in addition, the aspiration needle and the injection needle are both arranged on the side of the reaction cup deviating from the magnet adsorption mechanism above the reaction cup via the lifting bracket, thereby ensuring that the aspiration needle descends to the opposite side of the reaction cup where the magnetic beads are adsorbed and agglomerated when absorbing liquid, thereby avoiding the loss of magnetic beads.

[0025] Compared with the prior art, the present invention can effectively improve the cleaning effect and reduce the loss of magnetic beads without increasing the number of cleaning times, and has significant advantages such as reasonable structure, simple operation, and reliable operation.

Claims

1. A washing device for a chemical immunoassay optical analyzer, provided with a magnetic bead adsorption mechanism, characterized in that The magnetic bead adsorption mechanism is provided with a magnet mounting plate, on which more than two magnet mounting slots are provided, and in each magnet mounting slot two magnets are arranged side by side up and down, the magnetic pole directions of the two magnets in the same magnet mounting slot are reversed, and the magnetic attraction directions of the two magnets at the same height in the adjacent magnet mounting slots are also reversed; The magnet mounting plate is composed of a vertical plate and a bottom plate, the vertical plate and the bottom plate are vertically fixedly connected, and the cross section is L-shaped, the side of the vertical plate is provided with more than two U-shaped grooves for placing magnets, the opening direction of the U-shaped grooves is upward, the top of the vertical plate is provided with a pressing plate for closing the openings of the more than two U-shaped grooves, the pressing plate is fixedly connected to the top of the vertical plate by screws, and the bottom plate is provided with a splicing tooth for engaging with the rotating chassis; Two magnets are arranged side by side in the U-shaped groove on the side of the vertical plate, and a rust-proof iron plate is arranged on the outside of the magnet to strengthen the magnetism. The outside of the rust-proof iron plate is covered with a light-shielding tape to improve the light-shielding property of the entire mechanism. The vertical plate and the bottom plate are both in an arc shape adapted to the inner side of the rotating disk body. A reaction cup carrying track is provided on the rotating disk, and an installation notch that is used to assemble the magnet mounting plate and passes through the reaction cup carrying track is provided at a position corresponding to the washing position on the inner wall, and an installation groove that matches the splicing teeth is provided on the installation notch.

2. A washing device for a chemical immunoassay optical analyzer according to claim 1, characterized in that A pre-adsorption magnet installation groove is also provided on the inner side wall of the magnet installation plate or the rotating disk at a position corresponding to the starting position of the adsorption process, and a magnet is installed in the pre-adsorption magnet installation groove.

3. A washing device for a chemical immunoassay optical analyzer according to claim 1, characterized in that Two pre-adsorption magnet mounting grooves are arranged in parallel at the position corresponding to the starting position of the adsorption treatment on the magnet mounting plate along the rotation direction of the reaction cup rotating disk. A magnet is arranged in each pre-adsorption magnet mounting groove, which is used to pre-adsorb the magnetic beads in the reaction cup before the washing process starts, so as to improve the adsorption and cleaning effect.

4. A washing device for a chemical immunoassay optical analyzer according to claim 1, characterized in that A reaction cup rotating disk and a lifting needle rack are also provided. A channel for passing the sample is opened on the reaction cup rotating disk. The magnetic bead adsorption mechanism is arranged on the side of the channel, and the lifting needle rack is arranged above the channel.

5. A washing device for a chemical immunoassay optical analyzer according to claim 4, characterized in that The lifting needle rack is provided with at least two groups of injection and aspiration combination needles, and the injection and aspiration combination needles are composed of a aspiration needle and an injection needle, wherein the needle tip position of the lower end of the aspiration needle is lower than the needle tip position of the lower end of the injection needle, and the needle tip of the injection needle is bent towards the direction close to the aspiration needle.

6. A washing device for a chemical immunoassay optical analyzer according to claim 5, characterized in that The liquid aspiration needle and the liquid injection needle are both arranged on a side above the reaction cup and away from the magnet adsorption mechanism via a lifting needle frame.

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