A kind of Penicillium phospholyticum psf and its application

Abstract

The present invention relates to the technical field of microbial fertilizers, specifically a kind of Penicillium phospholyticum PSF, which is classified as PSF and belongs to Penicillium sp. On October 9, 2019, it was deposited in the General Microbiology Center of China Microbiological Culture Collection Management Committee. The culture preservation number is CGMCC No.18558. The preservation address is: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, postcode: 100101; Penicillium phospholyticum PSF bacterial strain provided by the invention, to Ca 5 (PO 4 ) 3 OH, AlPO 4 , Zn 3 (PO 4 ) 2 、FePO 4 A variety of insoluble phosphates have good solubility, and also have high salt tolerance, can grow and colonize well in saline soil, and promote the conversion of insoluble phosphorus into plants that can be absorbed and utilized. Soluble phosphate can promote the growth of plants, reduce the use of chemical fertilizers, and has good application and development prospects.

Description

technical field

[0001] The invention relates to the technical field of microbial fertilizers, in particular to a Penicillium phosphate solubilizing PSF strain, which is named Penicillium sp. by classification, and its application in improving the phosphorus supply level of salinized soil. Background technique

[0002] Phosphorus is one of the three major elements in plant nutrition and an important component of organic compounds in plants, which plays an important role in its growth and development. In my country, 74% of the cultivated land is deficient in soil phosphorus, and 95% of the phosphorus in the soil is in an ineffective form. In order to obtain high yield, a large amount of soluble phosphorus fertilizer is repeatedly applied to the soil every year. Only 5% to 10%, most of which are related to the Ca in the soil 2+ , Fe 2+ , Fe 3+ , A1 3+ Combine to form insoluble phosphates. This not only causes waste of phosphorus resources, but also poses a certain threat to...

Examples

Embodiment 1

[0031] A kind of Penicillium phosphatidylase PSF provided by the invention

[0032] 1. Origin of the strain

[0033] The sample is from the salinized soil of the Yellow River Delta;

[0034] Selected from the salinized soil of the Yellow River Delta, weighed 10 g of soil sample and placed it in 90 mL of sterile water, and shaken it for 3 minutes at a rotational speed of 3000 rpm to prepare a soil suspension;

[0035] 2. Isolation of strains

[0036] Dilute the soil suspension by a 10-fold gradient, and then pipette 10 -4 , 10 -5 , 10 -6 The three gradients of soil suspension were 200 μL, respectively, coated on the PVK inorganic phosphorus solid medium, and then the inoculated PVK inorganic phosphorus solid medium was inverted in a biochemical incubator, and incubated at 28 °C for 7 days. 15 strains of phosphate-dissolving fungi were screened out, among which the strain number is PSF with the strongest ability to dissolve phosphates, and the phosphate-dissolving circle fo...

Embodiment 2

[0049] Solubilization of Penicillium phosphatidylase PSF on various insoluble phosphates

[0050] This strain mainly targets four insoluble phosphates, namely Ca 5 (PO 4 ) 3 OH, AlPO 4 , Zn 3 (PO 4 ) 2 and FePO 4 Phosphorus solubilization;

[0051] Preparation of Penicillium phosphate solubilization PSF bacterial suspension: according to the volume fraction ratio, add 5 parts of PBS buffer to 1 part of Penicillium phosphate solubilization PSF, and mix evenly to prepare Penicillium phosphate solubilization PSF bacterial suspension, which is for later use;

[0052] Ca5(PO 4 )3OH, AlPO 4 , Zn 3 (PO 4 ) 2 , FePO 4 Take the preparation of Ca5(PO4)3OH phosphate detection solution as an example:

[0053] Add 50mL PVK liquid medium, 0.5g Ca to a 150mL Erlenmeyer flask 5 (PO 4 ) 3 OH, 500 μL Penicillium phosphatidylase PSF bacterial suspension, under the condition of temperature of 28 ℃, with a rotating speed of 200 r / min shaker for 5-7 d, to obtain Ca 5 (PO 4 ) 3 O...

Embodiment 3

[0060] Preparation of fermentation broth of Penicillium phosphatidylsis PSF

[0061] Penicillium phosphatidylsis PSF was inoculated on PDA medium, cultured at 28°C for 3 days, and the agar block containing PSF mycelium with a diameter of 7 mm was inoculated by punching method and placed in PVK liquid medium at a temperature of 28°C and shaken. The rotation speed was 180 rpm, and the shake flask was fermented for 96 hours to obtain the Penicillium phosphatidylase PSF fermentation broth, which was used for later use.

technical field

[0001] The invention relates to the technical field of microbial fertilizers, in particular to a Penicillium phosphate solubilizing PSF strain, which is named Penicillium sp. by classification, and its application in improving the phosphorus supply level of salinized soil. Background technique

[0002] Phosphorus is one of the three major elements in plant nutrition and an important component of organic compounds in plants, which plays an important role in its growth and development. In my country, 74% of the cultivated land is deficient in soil phosphorus, and 95% of the phosphorus in the soil is in an ineffective form. In order to obtain high yield, a large amount of soluble phosphorus fertilizer is repeatedly applied to the soil every year. Only 5% to 10%, most of which are related to the Ca in the soil 2+ , Fe 2+ , Fe 3+ , A1 3+ Combine to form insoluble phosphates. This not only causes waste of phosphorus resources, but also poses a certain threat to...

Examples

Embodiment 1

[0031] A kind of Penicillium phosphatidylase PSF provided by the invention

[0032] 1. Origin of the strain

[0033] The sample is from the salinized soil of the Yellow River Delta;

[0034] Selected from the salinized soil of the Yellow River Delta, weighed 10 g of soil sample and placed it in 90 mL of sterile water, and shaken it for 3 minutes at a rotational speed of 3000 rpm to prepare a soil suspension;

[0035] 2. Isolation of strains

[0036] Dilute the soil suspension by a 10-fold gradient, and then pipette 10 -4 , 10 -5 , 10 -6 The three gradients of soil suspension were 200 μL, respectively, coated on the PVK inorganic phosphorus solid medium, and then the inoculated PVK inorganic phosphorus solid medium was inverted in a biochemical incubator, and incubated at 28 °C for 7 days. 15 strains of phosphate-dissolving fungi were screened out, among which the strain number is PSF with the strongest ability to dissolve phosphates, and the phosphate-dissolving circle fo...

Embodiment 2

[0049] Solubilization of Penicillium phosphatidylase PSF on various insoluble phosphates

[0050] This strain mainly targets four insoluble phosphates, namely Ca 5 (PO 4 ) 3 OH, AlPO 4 , Zn 3 (PO 4 ) 2 and FePO 4 Phosphorus solubilization;

[0051] Preparation of Penicillium phosphate solubilization PSF bacterial suspension: according to the volume fraction ratio, add 5 parts of PBS buffer to 1 part of Penicillium phosphate solubilization PSF, and mix evenly to prepare Penicillium phosphate solubilization PSF bacterial suspension, which is for later use;

[0052] Ca5(PO 4 )3OH, AlPO 4 , Zn 3 (PO 4 ) 2 , FePO 4 Take the preparation of Ca5(PO4)3OH phosphate detection solution as an example:

[0053] Add 50mL PVK liquid medium, 0.5g Ca to a 150mL Erlenmeyer flask 5 (PO 4 ) 3 OH, 500 μL Penicillium phosphatidylase PSF bacterial suspension, under the condition of temperature of 28 ℃, with a rotating speed of 200 r / min shaker for 5-7 d, to obtain Ca 5 (PO 4 ) 3 O...

Embodiment 3

[0060] Preparation of fermentation broth of Penicillium phosphatidylsis PSF

[0061] Penicillium phosphatidylsis PSF was inoculated on PDA medium, cultured at 28°C for 3 days, and the agar block containing PSF mycelium with a diameter of 7 mm was inoculated by punching method and placed in PVK liquid medium at a temperature of 28°C and shaken. The rotation speed was 180 rpm, and the shake flask was fermented for 96 hours to obtain the Penicillium phosphatidylase PSF fermentation broth, which was used for later use.

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