Quick breeding method of Sichuan radix salvia miltiorrhiza stem tip detoxified seedlings

Abstract

The invention discloses a quick breeding method of Sichuan radix salvia miltiorrhiza stem tip detoxified seedlings. The quick breeding method comprises the steps of step 1, performing material selection and treatment; step 2, performing material disinfection; step 3, performing stripping and culturing of bacteria-free stem tips; step 4, performing virus detection and establishing of detoxified strains; step 5, performing enlarged proliferation of the detoxified strains; step 6, performing seedling strengthening and rooting of the detoxified seedlings; and step 7, performing seedling hardeningand transplanting of the detoxified seedlings. According to the method disclosed by the invention, a dedifferentiation-redifferentiation process of a callus tissue way is not performed, and massive high-quality Sichuan radix salvia miltiorrhiza seedlings not carrying viruses can be quickly cultivated, so that the cultivating time of the Sichuan radix salvia miltiorrhiza seedlings can be reduced, the genetic stability of excellent characters of the Sichuan radix salvia miltiorrhiza can be ensured, the survival rate of Sichuan radix salvia miltiorrhiza bacteria-free detoxified stem tips and thereproduction coefficient of seedlings can be greatly increased through optimized culture medium composition, quick obtaining of the seedlings can be realized, quick enlarged proliferation is realized,and demands of the market for massive superior Sichuan radix salvia miltiorrhiza seedlings can be met.

Description

technical field

[0001] The invention relates to the field of seedling breeding, in particular to a rapid breeding method for the virus-free seedlings of salvia miltiorrhiza. Background technique

[0002] Salvia miltiorrhiza Bunge is a perennial medicinal plant of the genus Salvia L. in the family Lamiaceae. Its roots and rhizomes are used as medicine. and other effects. Modern research has found that tanshinone and salvianolic acid compounds are its main medicinal chemical components. Clinically, Danshen and its pharmaceutical products are widely used in the treatment of cardiovascular diseases such as coronary heart disease and angina pectoris. Salvia miltiorrhiza is mainly produced in Sichuan, Shandong, Shaanxi and other places. Among them, salvia miltiorrhiza (Sichuan salvia miltiorrhiza) is a high-quality source of salvia miltiorrhiza because of its high content of phenolic acids in the root.

[0003] In recent years, the planting area and output of Sichuan Salvia mil...

Examples

Embodiment 1

[0035] The present embodiment provides a method for rapid propagation of virus-free seedlings of Salvia miltiorrhiza, comprising:

[0036] Step 1, material selection and processing: select robust Sichuan Salvia miltiorrhiza plants from the planting site of Sichuan Salvia miltiorrhiza at the end of August and mark them, cut off 3-4cm long terminal buds, and use a soft-bristled brush to dip the detergent into the cut terminal buds Scrub to remove impurities, then cut off the fully expanded leaves, rinse under running water for 20 minutes, and set aside;

[0037] Step 2, material disinfection: soak and sterilize the terminal buds treated in step 1 in 75% ethanol for 45 seconds in an ultra-clean workbench, wash them twice in sterile water, soak them in 0.1% mercuric chloride solution for 6 minutes, and then use Soak and wash in sterile water for 6 times to obtain sterile Sichuan salvia miltiorrhiza terminal buds;

[0038] Step 3, aseptic stem tip stripping and cultivation: the as...

Embodiment 2

[0044] The present embodiment provides a method for rapid propagation of virus-free seedlings of Salvia miltiorrhiza, comprising:

[0045] Step 1, material selection and processing: select robust Sichuan Salvia miltiorrhiza plants from the planting site of Sichuan Salvia miltiorrhiza at the end of August and mark them, cut off 3-4cm long terminal buds, and use a soft-bristled brush to dip the detergent into the cut terminal buds Scrub to remove impurities, then cut off the fully expanded leaves, rinse under running water for 20 minutes, and set aside;

[0046] Step 2, material disinfection: soak and sterilize the terminal buds treated in step 1 in 75% ethanol for 45 seconds in an ultra-clean workbench, wash them twice in sterile water, soak them in 0.1% mercuric chloride solution for 6 minutes, and then use Soak and wash in sterile water for 6 times to obtain sterile Sichuan salvia miltiorrhiza terminal buds;

[0047] Step 3, aseptic stem tip stripping and cultivation: the as...

Embodiment 3

[0053] The present embodiment provides a method for rapid propagation of virus-free seedlings of Salvia miltiorrhiza, comprising:

[0054] Step 1, material selection and processing: select robust Sichuan Salvia miltiorrhiza plants from the planting site of Sichuan Salvia miltiorrhiza at the end of August and mark them, cut off 3-4cm long terminal buds, and use a soft-bristled brush to dip the detergent into the cut terminal buds Scrub to remove impurities, then cut off the fully expanded leaves, rinse under running water for 20 minutes, and set aside;

[0055] Step 2, material disinfection: soak the terminal buds treated in step 1 in 75% ethanol for 60 seconds in an ultra-clean workbench, wash them twice in sterile water, soak them in 0.1% mercuric chloride solution for 8 minutes, and then use Soak and wash in sterile water for 6 times to obtain sterile Sichuan salvia miltiorrhiza terminal buds;

[0056] Step 3, aseptic stem tip stripping and cultivation: the aseptic Sichuan ...

technical field

[0001] The invention relates to the field of seedling breeding, in particular to a rapid breeding method for the virus-free seedlings of salvia miltiorrhiza. Background technique

[0002] Salvia miltiorrhiza Bunge is a perennial medicinal plant of the genus Salvia L. in the family Lamiaceae. Its roots and rhizomes are used as medicine. and other effects. Modern research has found that tanshinone and salvianolic acid compounds are its main medicinal chemical components. Clinically, Danshen and its pharmaceutical products are widely used in the treatment of cardiovascular diseases such as coronary heart disease and angina pectoris. Salvia miltiorrhiza is mainly produced in Sichuan, Shandong, Shaanxi and other places. Among them, salvia miltiorrhiza (Sichuan salvia miltiorrhiza) is a high-quality source of salvia miltiorrhiza because of its high content of phenolic acids in the root.

[0003] In recent years, the planting area and output of Sichuan Salvia mil...

Examples

Embodiment 1

[0035] The present embodiment provides a method for rapid propagation of virus-free seedlings of Salvia miltiorrhiza, comprising:

[0036] Step 1, material selection and processing: select robust Sichuan Salvia miltiorrhiza plants from the planting site of Sichuan Salvia miltiorrhiza at the end of August and mark them, cut off 3-4cm long terminal buds, and use a soft-bristled brush to dip the detergent into the cut terminal buds Scrub to remove impurities, then cut off the fully expanded leaves, rinse under running water for 20 minutes, and set aside;

[0037] Step 2, material disinfection: soak and sterilize the terminal buds treated in step 1 in 75% ethanol for 45 seconds in an ultra-clean workbench, wash them twice in sterile water, soak them in 0.1% mercuric chloride solution for 6 minutes, and then use Soak and wash in sterile water for 6 times to obtain sterile Sichuan salvia miltiorrhiza terminal buds;

[0038] Step 3, aseptic stem tip stripping and cultivation: the as...

Embodiment 2

[0044] The present embodiment provides a method for rapid propagation of virus-free seedlings of Salvia miltiorrhiza, comprising:

[0045] Step 1, material selection and processing: select robust Sichuan Salvia miltiorrhiza plants from the planting site of Sichuan Salvia miltiorrhiza at the end of August and mark them, cut off 3-4cm long terminal buds, and use a soft-bristled brush to dip the detergent into the cut terminal buds Scrub to remove impurities, then cut off the fully expanded leaves, rinse under running water for 20 minutes, and set aside;

[0046] Step 2, material disinfection: soak and sterilize the terminal buds treated in step 1 in 75% ethanol for 45 seconds in an ultra-clean workbench, wash them twice in sterile water, soak them in 0.1% mercuric chloride solution for 6 minutes, and then use Soak and wash in sterile water for 6 times to obtain sterile Sichuan salvia miltiorrhiza terminal buds;

[0047] Step 3, aseptic stem tip stripping and cultivation: the as...

Embodiment 3

[0053] The present embodiment provides a method for rapid propagation of virus-free seedlings of Salvia miltiorrhiza, comprising:

[0054] Step 1, material selection and processing: select robust Sichuan Salvia miltiorrhiza plants from the planting site of Sichuan Salvia miltiorrhiza at the end of August and mark them, cut off 3-4cm long terminal buds, and use a soft-bristled brush to dip the detergent into the cut terminal buds Scrub to remove impurities, then cut off the fully expanded leaves, rinse under running water for 20 minutes, and set aside;

[0055] Step 2, material disinfection: soak the terminal buds treated in step 1 in 75% ethanol for 60 seconds in an ultra-clean workbench, wash them twice in sterile water, soak them in 0.1% mercuric chloride solution for 8 minutes, and then use Soak and wash in sterile water for 6 times to obtain sterile Sichuan salvia miltiorrhiza terminal buds;

[0056] Step 3, aseptic stem tip stripping and cultivation: the aseptic Sichuan ...