Liquid detection system

Abstract

The present invention provides a liquid detection system, the detection system includes a chip and a frame, the chip includes a shell, a cover, a first base and a multi-channel selector valve; the linkage part of the multi-channel selector valve passes through the first base and the shell, and has a first matching part at the upper end; the detection system also includes: a bearing member is arranged on the frame and is suitable for carrying the chip; a fixed plate has a first through hole and is arranged on a guide rail through a sliding member; when the fixed plate is in a working position, the first through hole and the first matching part correspond to each other up and down; the guide rail is arranged on the frame; a first motor is fixed on the fixed plate; a transmission member is driven by the first motor and passes through the first through hole; when the fixed plate is in a working position, a second matching part at the bottom end of the transmission member matches with the first matching part and drives the linkage part to rotate. The present invention has the advantages of simple structure and automation.

Description

Technical Field

[0001] The invention relates to liquid detection, and in particular to a system for mixing, conveying and detecting multiple liquids. Background Art

[0002] In various analyses, samples and multiple reagents need to be added to a mixing chamber, such as a test tube. These samples can be human and animal samples, such as whole blood, plasma, serum, urine, saliva, feces, cerebrospinal fluid or lavage fluid, wax-sealed tissues and slices, tissue homogenates, plants, including Chinese medicine, food raw materials, processed foods, meat, beverages and alcoholic beverages, and raw materials and semi-finished products in the manufacturing process of beverages, as well as cell culture fluids or pathogenic virus culture fluids. The types of components analyzed include minerals, inorganic substances, organic substances, nucleic acids DNA, RNA, proteins or fats. At present, the common liquid detection systems are:

[0003] The chip has a liquid selection structure, a mixing structure and a conveying structure; the selection structure has a rotating disk and a motor, and the rotating disk has a channel that selectively connects the liquid cavity when it rotates; when the chip is fixed, the motor shaft moves downward, and the bottom end of the shaft cooperates with the rotating disk, thereby driving the rotating disk to select different liquid cavities. This technical solution has many shortcomings, such as:

[0004] 1. The shaft is connected to the rotating disk from top to bottom. If there is a slight deviation, the two cannot cooperate, which increases the requirements for motor positioning.

[0005] 2. The up and down movement structure of the motor is complex. Summary of the invention

[0006] In order to solve the deficiencies in the above-mentioned prior art solutions, the present invention provides a liquid detection system with simple structure and high working efficiency.

[0007] The objective of the present invention is achieved through the following technical solutions:

[0008] A liquid detection system, the liquid detection system comprises a chip and a frame, the chip comprises a shell, a cover, a first base and a multi-channel directional selection valve; the shell has a plurality of liquid chambers, the cover is buckled on the top of the shell, the multi-channel directional selection valve is fixed on the first base and blocked by the first base; the rotating part of the multi-channel directional selection valve passes through the first base and the shell, and has a first matching part at the upper end; the liquid detection system also comprises:

[0009] A carrier, the carrier is arranged on the frame and is suitable for carrying the chip;

[0010] A fixed plate, the fixed plate having a first through hole and being arranged on the guide rail through a sliding member; when the fixed plate is in a working position, the first through hole and the first matching portion correspond to each other up and down;

[0011] A guide rail, the guide rail being horizontally arranged on the frame;

[0012] a first motor, wherein the first motor is fixed to the fixing plate;

[0013] A transmission member is driven by the first motor and passes through the first through hole; when the fixed plate is in the working position, the second matching portion at the bottom end of the transmission member matches with the first matching portion and drives the rotating member to rotate.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. Simple structure;

[0016] The mobile platform of the patent application: a combination of a fixed plate, a sliding member and a horizontal guide rail, realizes accurate connection and separation between the transmission member and the chip, and facilitates the insertion and removal of the chip;

[0017] The first mating part and the second mating part are matched with a keyway, which has a simple structure and good reliability;

[0018] 2. High work efficiency;

[0019] The cover body is provided with a second groove extending to the rotating member, which is suitable for inserting and pulling out the safety pin on the one hand, realizing the safety function, and is suitable for the bottom end of the transmission member to move forward and backward in the second groove on the other hand; when moving forward, the bottom end of the transmission member first passes through the second groove, and then cooperates with the rotating member, thereby driving the multi-channel selector valve to work; when moving backward, the bottom end of the transmission member moves out of the rotating member and the second groove, thereby taking out the used chip and putting in the new chip, and the cooperation is fast and accurate, and no manual alignment is required;

[0020] 3. Good working performance;

[0021] The design of different widths of the first matching parts and the corresponding design of different widths of the second matching parts help the transmission member to match the rotating member tightly, thereby improving the positioning accuracy of the multi-channel directional selector valve. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The disclosure of the present invention will become easier to understand with reference to the accompanying drawings. It is easy for those skilled in the art to understand that these drawings are only used to illustrate the technical solution of the present invention and are not intended to limit the scope of protection of the present invention. In the drawings:

[0023] Figure 1 It is a simplified structural diagram of the detection system of the present invention;

[0024] Figure 2 It is a simplified structural diagram of the detection system of the present invention. DETAILED DESCRIPTION

[0025] Figure 1-2 The following description describes the optional embodiments of the present invention to teach those skilled in the art how to implement and reproduce the present invention. In order to teach the technical solution of the present invention, some conventional aspects have been simplified or omitted. It should be understood by those skilled in the art that variations or substitutions derived from these embodiments will be within the scope of the present invention. It should be understood by those skilled in the art that the following features can be combined in various ways to form multiple variations of the present invention. Thus, the present invention is not limited to the following optional embodiments, but is only limited by the claims and their equivalents.

[0026] Embodiment 1:

[0027] Figure 1-2 The basic structure diagram of the liquid detection system of the embodiment of the present invention is schematically shown as follows: Figure 1-2 As shown, the liquid detection system comprises:

[0028] A frame, which is used to carry components such as chips and guide rails;

[0029] A carrier, which is arranged on the frame and is suitable for carrying the chip; for example, the bottom end of the carrier is two V-shaped detection grooves, which are suitable for inserting and fixing the two V-shaped detection cavities of the chip;

[0030] A fixing plate 41, the fixing plate 41 having a first through hole and being arranged on a guide rail 43 through a sliding member 42; when the fixing plate 41 is in the working position, the first through hole and the first groove (first matching portion) of the linkage portion 21 of the chip (with the safety pin pulled out) on the carrier correspond to each other up and down;

[0031] A guide rail 43, the guide rail 43 is horizontally arranged at the top end of the frame and extends along the front-rear direction;

[0032] A first motor 31, wherein the first motor is fixed on the fixing plate, and an encoder is connected to the rotating shaft of the first motor and is used to determine the position of the rotating shaft of the first motor;

[0033] Transmission member 32, the transmission member passes through the first through hole, and the transmission member is driven by the first motor; when the fixed plate is in the working position, the key (second matching part) at the bottom end of the transmission member matches with the first groove of the linkage part of the chip; in the vertical direction, the bottom end position of the transmission member is not lower than the height of the first groove, and is not higher than the top height of the linkage part; when the fixed plate moves forward along the guide rail, the bottom end of the transmission member moves forward in the second groove of the cover body and is stuck in the first groove; when the fixed plate moves backward along the guide rail, the bottom end of the transmission member moves out of the first groove and the second groove in turn, so as to take out the detected chip and load a new chip;

[0034] A second motor, the second motor is fixed to the fixed plate, driving the connecting member to move up and down, thereby driving the moving member to move up and down;

[0035] A connecting member, wherein the upper portion of the connecting member is located on the upper side of the fixed plate, and the lower portion of the connecting member passes through the fourth through hole of the fixed plate and is connected to the moving member on the lower side of the fixed plate;

[0036] A moving member, wherein the moving member is arranged at the lower side of the fixed plate, and the upper side of the chip moves up and down under the driving of the moving member;

[0037] Guide members, at least two cylindrical guide members are fixed on the upper side of the moving member;

[0038] Guide holes, the number of which matches the number of the guide members, are arranged on the fixing plate and are suitable for the guide members to pass through;

[0039] The chip includes a cover 11, a shell, a first base and a second base arranged from top to bottom;

[0040] A housing, wherein the housing has a cylindrical channel, a plurality of liquid chambers are arranged around the cylindrical channel, and are respectively used to contain samples and reagents, and the bottom of the liquid chamber has outlets distributed along the outer side of the cylindrical channel, such as in concentric circles or on non-same circles, and each outlet has a film that closes the liquid chamber; the housing has a sealing member that closes the plurality of liquid chambers from the top, and the sealing member has a through hole that is connected to the inside of the liquid chamber, suitable for adding samples, etc.;

[0041] A safety pin, wherein the safety pin has a horizontal portion, the length of the horizontal portion is greater than the length of the second groove, that is, the horizontal portion extends outside the second groove, suitable for the operator to pull out the safety pin; the horizontal portion has two side portions extending along the length direction at opposite positions and having strip-shaped protrusions extending outward, such as having strip-shaped protrusions at the bottom end or the middle of the two side portions (the left and right side portions of the horizontal portion, not the upper and lower side portions and the front and rear side portions), and the strip-shaped protrusions extend continuously or intermittently along the length direction; the two side portions have a first group of protrusions suitable for the end portion of the elastic portion to be snapped into The groove is a vertical groove or an inclined groove extending in the up-down direction; the width of the end of the horizontal part becomes smaller, suitable for inserting into the first groove of the linkage part; for the sake of aesthetics, the upper end of the end also has a sheet-like protrusion extending outward and along the length direction; in order to facilitate the extraction of the safety pin, the top of the sheet-like protrusion further has a plurality of protrusions extending perpendicular to the length direction (i.e., the width direction); the horizontal part adopts a non-solid hollow structure; the safety pin fixed in the second groove prevents the housing from moving downward, and the sheet-like protrusion covers the top of the linkage part;

[0042] A cover body 11 is located on the upper side of the shell, and closes the upper end through hole of the shell, such as by inserting a protrusion into the through hole to close it, so that the liquid cavity in the shell becomes a closed environment; a second groove 12 is provided in the cover body, and the second groove extends to the cylindrical channel; the linkage part of the rotating body of the first base passes through the cylindrical channel, and the upper end is in the second groove; the two opposite walls of the second groove have wings arranged oppositely, and the distance between the two wings is less than the width of the second groove; the second groove is suitable for the insertion of the strip protrusion, and the two wings block the strip protrusion from moving toward The horizontal part is inserted between the two wings; the sides of the two wings facing the safety pins are respectively provided with suspended elastic parts, such as the elastic parts processed on the wings; in a free state, the distance between the two elastic parts in opposite positions is smaller than the width of the part with the strip-shaped protrusion of the horizontal part; when the horizontal part is inserted into the groove of the closure, the part of the horizontal part with a gradually decreasing width passes between the two elastic parts, the distance between the two elastic parts is gradually expanded, and finally is stuck in the first group of grooves of the two side parts;

[0043] The first base has a third through hole, a second opening and a third opening. The bottom end of the first base has a first opening and a first group of openings that are concentric circles around the third through hole. The upper end has a second group of openings and an eighth opening that are concentric circles or on non-same circumferences around the third through hole. The upper end of the second group of openings has a hollow pointed protrusion that is suitable for piercing the film and inserting into the outlet at the lower end of the liquid cavity. The first base has a plurality of mutually isolated channels that are used to connect the first group of openings and the second group of openings, respectively, so that the first group of openings corresponds to the liquid cavity one by one and are internally connected. The inner diameter of the channel is small, so that the liquid in the liquid cavity cannot flow to the first group of outlets autonomously by its own gravity alone. The first base also has a second opening and a third opening that are respectively connected to the eighth outlet. The lower end of the first base has a fifth groove, such as a circular groove, and the third through hole is located in the fifth groove.

[0044] A multi-channel directional selector valve, the multi-channel directional selector valve comprising a rotating body, the rotating body comprising:

[0045] The linkage part, the rotating disk and the hollow rotating member are colinear with the central axis from top to bottom; the linkage part passes through the third through hole and is in the cylindrical channel; the upper end of the part of the linkage part in the second groove has a first groove; in order to cooperate with the transmission member, preferably, in the horizontal extension direction of the second groove, the width of the first groove becomes larger: the rotating disk includes a first part with a larger radius (larger than the third through hole) and a second part with a smaller radius, and the first part and the second part are accommodated in the fifth groove; the upper end of the rotating disk has a fourth opening, and the interior of the rotating disk has a second channel connected to the interior of the rotating member; when the rotating disk rotates, the fourth opening is connected to the first opening and the first group of openings respectively, so as to realize the selective connection with the first opening and the first group of openings, that is, to realize the selective connection between the interior of the rotating member and the liquid cavity;

[0046] The second base, the lower end of the second base has a cavity, and the cross-sectional area of ​​the cavity gradually increases from bottom to top; the second base on the upper part of the cavity has a second through hole and a circular groove surrounding the second through hole; the rubber O-ring is sleeved on the rotating member and is in the circular groove, so as to achieve the sealing between the rotating member and the cavity; the outer periphery of the circular groove has an annular protrusion, the second part is in the space surrounded by the annular protrusion and the second base, and the first part is blocked by the annular protrusion; the annular protrusion is in the fifth groove, and the rotating disk is in the space surrounded by the fifth groove, the annular protrusion and the second base; the rotating member extends into the cavity through the second through hole, and the distance between the bottom end of the rotating member and the bottom wall of the cavity is less than 1mm, such as 0.5mm; the lower end of the second base outside the cavity has a fifth opening, and a filter membrane is provided at the fifth opening, and a third channel is provided in the second base for connecting the cavity and the fifth opening;

[0047] an air pump, the air pump being connected to the fifth opening;

[0048] The storage device includes a main body and a film, wherein the main body has a detection cavity, and the film is attached to both sides of the main body; the main body has a convex sixth opening and a convex seventh opening respectively connected to the detection cavity; the sixth opening and the seventh opening are inserted into the flexible material in the fourth groove, and are connected to the second opening and the third opening;

[0049] Before the formal test, the distance between the shell and the first base is greater than zero, and the second group of openings is not connected to the liquid chamber; at the same time, since the safety pin is inserted into the first groove of the linkage part, the shell cannot move downward;

[0050] a light source that emits light of multiple wavelengths;

[0051] A first selection module, the first selection module is used to select excitation lights of different wavelengths from the lights of the multiple wavelengths, and the excitation lights are respectively incident into the detection cavities in the detection slots;

[0052] a second selection module, the second selection module being used to select light of different wavelengths from the light emitted in the detection cavity;

[0053] A detector that converts the emitted light of different wavelengths into an electrical signal;

[0054] An incident optical fiber, which transmits light emitted by different light sources into the detection cavity, and an output end of the incident optical fiber is fixed on the V-shaped groove;

[0055] An output optical fiber, which transmits the light in the detection cavity to different detectors, and the input end of the output optical fiber is fixed on the V-shaped groove;

[0056] The temperature control unit comprises a body, a heater and a refrigerator. The body is made of heat-conducting materials such as aluminum and copper, and has a sixth groove suitable for the cavity to be placed in. The heater is a sheet-shaped heating ring, which is arranged in the annular groove on the lower side of the body. The refrigerator is a TEC, with a cold surface attached to the body and heat dissipation teeth and a heat dissipation fan arranged on the hot surface.

[0057] The electromagnet and the ultrasonic module are arranged on the lower side of the body and are located on the lower side of the cavity;

[0058] A temperature control channel is formed in a rectangular channel surrounded by iron sheets, one end of the temperature control channel is connected to a fan; and an electric heating plate is arranged outside the detection tank.

[0059] The detection method of this application example includes a preparation stage, a mixing stage, a conveying stage and a detection stage; the preparation stage includes the following steps:

[0060] (A1) pushing the fixed plate backward, and the fixed plate moves backward along the guide rail;

[0061] Pull out the safety pin on the chip, the safety pin is separated from the first groove and the second groove of the cover body in turn, the distance between the shell and the first base is greater than zero, the liquid channel in the first base such as the second channel is not connected to the liquid cavity in the shell, and then the chip is placed on the carrier on the lower side of the fixed plate, the two V-shaped grooves of the carrier support the two V-shaped detection cavities of the storage device, such as Figure 2 As shown;

[0062] (A2) pulling the fixing plate forward, and the fixing plate moves forward along the guide rail;

[0063] The transmission member passing through the fixed plate moves forward in a direction parallel to the extension direction of the guide rail, and the bottom end of the transmission member moves forward in the second groove, thereby being inserted into the first groove at the upper end of the linkage part of the multi-channel selector valve, so that the first motor drives the linkage part and the rotating disk to rotate through the transmission member. Figure 1 As shown;

[0064] (A3) The second motor rotates to drive the connecting member to move downward, thereby pushing the cover body to move downward, and the downwardly moving shell body is buckled with the fixed first base, and the second group of openings enters the outlet of the liquid cavity, puncturing the film, so that the liquid channel in the first base and the liquid cavity in the shell body are connected;

[0065] The mixing stage comprises the following steps:

[0066] The first motor drives the linkage part, the rotating disk and the rotating member to rotate by using the transmission member, and uses the encoder to position so that the fourth opening on the rotating disk selectively communicates with the first group of openings; in this state, the liquid cavity is selectively connected with the second group of openings, the first group of openings, the fourth opening, the rotating disk and the channels and cavities in the rotating member, but due to the resistance of the narrow channel, the liquid cannot flow out of the liquid cavity to the first group of openings by gravity alone; at the same time, the liquid in the liquid cavity that is not connected with the fourth opening cannot flow out to the first group of openings by its own gravity alone;

[0067] When the air pump is working, the gas in the cavity is discharged from the third channel and the fifth opening in sequence, and the cavity becomes negative pressure; the liquid in the liquid cavity breaks through and passes through the second group of openings, the channels in the first base, the first group of openings, the fourth opening, the second channel and the channels in the rotating member in sequence and enters the cavity, thereby realizing that multiple liquids (samples and reagents) are selectively extracted from the liquid cavity and mixed in the cavity;

[0068] During the reaction of the mixed liquid in the cavity, the temperature control unit maintains the set temperature of the mixed liquid through the heater and the refrigerator;

[0069] When there are magnetic beads in the mixed liquid, the electromagnet is used to adsorb the magnetic beads, and the magnetic beads gather at the bottom of the cavity; the ultrasonic module is used to disperse the gathered magnetic beads.

[0070] The delivery phase includes the following steps:

[0071] The first motor drives the linkage part to rotate, and through the positioning of the encoder, the fourth opening on the rotating rotating disk is connected to the first opening;

[0072] Inject gas into the cavity through the air pump and the fifth opening, so that the liquid surface in the cavity is under positive pressure, and the mixed liquid in the cavity is pressed to sequentially pass through the hollow rotating member, the second channel, the fourth opening, the first opening, the first channel, and then enter the detection cavity in the storage device through the second opening and the third opening;

[0073] The detection phase includes the following steps:

[0074] (B1) Excitation lights of different wavelengths emitted by multiple light sources are injected into each detection cavity through an incident optical fiber, and the first switching module is used to allow the excitation lights output by some different light sources to enter the detection cavity respectively; the second switching module is used to allow the output lights in different detection cavities to enter the detector for reception, and the parameters of the sample are obtained after analysis;

[0075] The temperature of the liquid in the detection chamber is increased by using an electric heating plate, and the temperature of the liquid in the detection chamber is lowered by using the airflow generated by the fan, so that the liquid in the detection chamber maintains a set temperature.

[0076] Embodiment 2:

[0077] An example of application of the liquid detection system according to Example 1 of the present invention in fluorescent quantitative PCR.

[0078] In this application example, the liquid detection system includes:

[0079] A chip, the chip comprising a cover body 11, a shell, a first base and a second base arranged from top to bottom;

[0080] A shell, wherein the shell has a cylindrical channel, and a plurality of liquid chambers are arranged around the cylindrical channel, respectively used to contain samples and reagents, the bottom of the liquid chamber has outlets distributed in concentric circles along the central axis of the cylindrical channel, and each outlet has a film that closes the liquid chamber; the shell has a sealing member that closes the plurality of liquid chambers from the top, and the sealing member has a through hole that communicates with the inside of the liquid chamber, suitable for adding samples, etc.;

[0081] A safety pin, wherein the safety pin has a horizontal portion, the length of the horizontal portion is greater than the length of the second groove, that is, the horizontal portion extends outside the second groove and the operator pulls out the safety pin; the two side portions of the horizontal portion extending in the length direction have strip-shaped protrusions extending outward, such as strip-shaped protrusions at the bottom end or the middle of the two side portions (the left and right side portions of the horizontal portion, not the upper and lower side portions and the front and rear side portions), and the strip-shaped protrusions extend continuously or discontinuously along the length direction; the two side portions have a first group of grooves suitable for the end portion of the elastic portion to be inserted, such as a vertical groove or an inclined groove extending in the up and down direction; the width of the end portion of the horizontal portion becomes smaller and is suitable for inserting into the first groove of the linkage portion; the upper end of the end portion has a sheet-shaped protrusion extending outward and along the length direction; in order to facilitate the extraction of the safety pin, the top end of the sheet-shaped protrusion further has a plurality of protrusions extending perpendicularly to the length direction (i.e., the width direction); the horizontal portion adopts a non-solid hollow structure;

[0082] A cover body 11 is located on the upper side of the shell, and closes the upper end through hole of the shell, such as by inserting a protrusion into the through hole to close it, so that the liquid cavity in the shell becomes a closed environment; a second groove 12 is provided in the cover body, and the second groove extends to the cylindrical channel; the linkage part of the rotating body of the first base passes through the cylindrical channel, and the upper end is in the second groove; the two opposite walls of the second groove have wings arranged oppositely, and the distance between the two wings is less than the width of the second groove; the second groove is suitable for the insertion of the strip-shaped protrusion, and the two wings block the strip-shaped protrusion The horizontal part is inserted between the two wings; the sides of the two wings facing the safety pins are respectively provided with suspended elastic parts, such as the elastic parts processed on the wings; in a free state, the distance between the two elastic parts in opposite positions is smaller than the width of the part with the strip-shaped protrusion of the horizontal part; when the horizontal part is inserted into the groove of the closure, the part of the horizontal part with a gradually decreasing width passes between the two elastic parts, the distance between the two elastic parts is gradually expanded, and finally is inserted into the first group of grooves of the two side parts;

[0083] The first base has a third through hole, a second opening and a third opening. The bottom end of the first base has a first opening and a first group of openings that are concentric circles around the third through hole. The upper end has a second group of openings and an eighth opening that are concentric circles around the third through hole. The upper end of the second group of openings has a hollow pointed protrusion that is suitable for piercing the film and inserting into the outlet at the lower end of the liquid cavity. The first base has a plurality of mutually isolated channels that are used to connect the first group of openings and the second group of openings, respectively, so that the first group of openings corresponds to the liquid cavity one by one and are internally connected. The inner diameter of the channel is small, so that the liquid in the liquid cavity cannot flow to the first group of outlets autonomously by relying solely on its own gravity. The upper end of the first base also has a ninth opening and a tenth opening. The eighth opening is connected to the first opening. The ninth opening is connected with the second opening through the channel in the first base, and the tenth opening is connected with the third opening through the channel in the first base; the upper end of the first base has an annular protrusion, and the second group of openings, the eighth opening, the ninth opening and the tenth opening are located in the third groove surrounded by the annular protrusion; one side of the connecting piece has a "Y"-shaped groove, which is buckled in the third groove, so that a "Y"-shaped channel isolated from the outside is formed between the eighth opening and the ninth opening and the tenth opening; the second opening and the third opening are arranged in the fourth groove at the lower end of the first base, and outside the rotation radius of the rotating disk, the fourth groove has a flexible material; the lower end of the first base has a fifth groove, and the third through hole is located in the fifth groove;

[0084] A multi-channel directional selector valve, the multi-channel directional selector valve comprising a rotating body, the rotating body comprising:

[0085] The linkage part 21, the rotating disk and the hollow rotating member are colinear with the central axis from top to bottom; the linkage part passes through the third through hole and is in the cylindrical channel; the upper end of the part of the linkage part in the second groove has a first groove; in the horizontal extension direction of the second groove, the width of the first groove becomes larger: in this application example, the linkage part is cylindrical and has two grooves of different widths; the rotating disk includes a first part with a larger radius (larger than the third through hole) and a second part with a smaller radius, and the first part and the second part are accommodated in the fifth groove; the upper end of the rotating disk has a fourth opening, and the interior of the rotating disk has a second channel connected to the interior of the rotating member; when the rotating disk rotates, the fourth opening is connected to the first opening and the first group of openings respectively, so as to realize the selective connection of the first opening and the first group of openings, that is, to realize the selective connection of the interior of the rotating member with the liquid cavity; the end of the horizontal part is inserted into the first groove of the linkage part: the groove with a larger width is inserted first, and then the groove with a smaller width is inserted, and the safety pin fixed in the second groove prevents the downward movement of the shell, and the sheet-like protrusion covers the top of the linkage part;

[0086] The second base, the lower end of the second base has a cavity, and the cross-sectional area of ​​the cavity gradually increases from bottom to top; the second base at the upper part of the cavity has a second through hole and a circular groove surrounding the second through hole; the rubber O-ring is sleeved on the rotating member and is in the circular groove, so as to achieve the sealing between the rotating member and the cavity; the outer periphery of the circular groove has an annular protrusion, the second part is in the space surrounded by the annular protrusion and the second base, and the first part is blocked by the annular protrusion; the annular protrusion is in the fifth groove, and the rotating disk is in the space surrounded by the fifth groove, the annular protrusion and the second base; the rotating member extends into the cavity through the second through hole, and the distance between the bottom end of the rotating member and the bottom wall of the cavity is less than 1mm, such as 0.5mm; the lower end of the second base outside the cavity has a convex fifth opening, and the fifth opening has a filter membrane, and the second base has a third channel for connecting the cavity and the fifth opening;

[0087] an air pump, the air pump being connected to the fifth opening;

[0088] The storage device includes a main body and a film, wherein the main body has a detection cavity, and the film is attached to both sides of the main body; the main body has a convex sixth opening and a convex seventh opening respectively connected to the detection cavity; the sixth opening and the seventh opening are inserted into the flexible material in the fourth groove, and are connected to the second opening and the third opening;

[0089] Before the formal test, the distance between the shell and the first base is greater than zero, and the second group of openings is not connected to the liquid chamber; at the same time, since the safety pin is inserted into the groove of the linkage part, the shell cannot move downward;

[0090] A frame, the frame is used to carry the chip, fixing plate and other components;

[0091] A carrier, the carrier is arranged on the frame and is suitable for carrying the chip; the bottom end of the carrier is two V-shaped detection grooves, suitable for inserting and fixing the two V-shaped detection cavities of the main body;

[0092] A fixing plate 41, the fixing plate 41 having a first through hole and being arranged on a guide rail 43 through a sliding member 42; when the fixing plate is in a working position, the first through hole and the first groove of the chip (with the safety pin removed) on the carrier correspond vertically;

[0093] A guide rail 43, which is arranged at the top end of the frame and extends along the front-rear direction;

[0094] A first motor 31, wherein the first motor is fixed on the fixing plate, and an encoder is connected to the rotating shaft of the first motor and is used to determine the position of the rotating shaft of the first motor;

[0095] Transmission member 32, the transmission member passes through the first through hole, and the transmission member is driven by the first motor; when the fixed plate is in the working position, the bottom end of the transmission member is stuck in the first groove; corresponding to the first groove, in the horizontal direction, the width from one end to the other end of the bottom end of the transmission member becomes larger; in the vertical direction, the bottom end position of the transmission member is not lower than the height of the first groove, and is not higher than the top height of the linkage part; when the fixed plate moves forward along the guide rail, the bottom end of the transmission member moves forward in the second groove and is stuck in the first groove; when the fixed plate moves backward along the guide rail, the bottom end of the transmission member moves out of the first groove and the second groove in turn, so as to take out the detected chip and load a new chip;

[0096] a second motor, the second motor being fixed to the fixing plate;

[0097] A connecting plate, the upper portion of which is located on the upper side of the fixed plate and has a fifth through hole (rectangular through hole), and the lower portion of which passes through the fourth through hole of the fixed plate and is fixedly connected to the moving member on the lower side of the fixed plate;

[0098] An eccentric wheel, wherein the eccentric wheel is arranged in the fifth through hole, and the rotating shaft of the second motor is connected to the eccentric wheel; the rotating eccentric wheel drives the connecting plate to move up and down: when the eccentric wheel rotates against the bottom wall of the fifth through hole, the connecting plate and the moving part are driven to move downward, and when the eccentric wheel rotates against the top wall of the fifth through hole, the connecting plate and the moving part are driven to move upward;

[0099] A moving member, wherein the moving member (moving plate) is arranged on the lower side of the fixed plate, and the upper side of the chip has a sixth through hole suitable for the transmission member to pass through;

[0100] Guide members, at least two cylindrical guide members are fixed on the upper side of the moving member;

[0101] Guide holes, the number of which matches the number of the guide members, are arranged on the fixing plate and are suitable for the guide members to pass through;

[0102] A guide component, wherein the guide component is arranged on the upper side of the fixed plate, and the guide member is adapted to pass through the guide hole and the interior of the guide component, thereby limiting the movable member to be able to move up and down only perpendicularly to the guide member;

[0103] The first selection module is shared by the second selection module, and the first selection module includes:

[0104] A rotating member, wherein the rotating member has mounting positions distributed in concentric circles around a rotating axis;

[0105] a third motor, wherein the third motor drives the rotating member to rotate;

[0106] A plurality of detection units, wherein the detection units include:

[0107] The transmitting module and the detecting module are arranged at intervals and fixed on the mounting positions respectively; the light source, the collimating lens group and the filter are respectively arranged in the transmitting module, and the collimating lens group, the filter and the detector are arranged in the detecting module; when the transmitting module rotating with the rotating part corresponds to the incident optical fiber, the adjacent detecting module corresponds to the output optical fiber; the transmitting module of each group of detection units emits excitation light of different wavelengths;

[0108] An incident optical fiber, which transmits light emitted by light sources in different emission modules to the detection cavity, and an output end of the incident optical fiber is fixed on the V-shaped groove;

[0109] An output optical fiber, wherein the output optical fiber transmits the light in the detection cavity to different detection modules, and the input end of the output optical fiber is fixed on the V-shaped groove; by rotating, different detection units correspond to the incident optical fiber and the output optical fiber respectively, that is, the excitation light of different wavelengths enters the detection cavity through the incident optical fiber, and the fluorescence of different wavelengths excited in the detection cavity is sent to the detection module for reception through the output optical fiber; in this embodiment, four groups of detection units are used, so that the excitation light of four different wavelengths are respectively incident on two detection cavities, and the fluorescence of different wavelengths emitted from the detection cavity is respectively received by the detection modules;

[0110] The temperature control unit comprises a body, a heater and a refrigerator. The body is made of heat-conducting materials such as aluminum and copper, and has a sixth groove suitable for the cavity to be placed in. The heater is a sheet-shaped heating ring, which is arranged in the annular groove on the lower side of the body. The refrigerator is a TEC, with a cold surface attached to the body and heat dissipation teeth and a heat dissipation fan arranged on the hot surface.

[0111] The electromagnet and the ultrasonic module are arranged on the lower side of the body and are located on the lower side of the cavity;

[0112] A temperature control channel is formed in a rectangular channel surrounded by iron sheets, one end of the temperature control channel is connected to a fan; and an electric heating plate is arranged outside the detection tank.

[0113] The detection method of this application example includes a preparation stage, a mixing stage, a conveying stage and a detection stage; the preparation stage includes the following steps:

[0114] (A1) pushing the fixed plate backward, and the fixed plate moves backward along the guide rail;

[0115] Pull out the safety pin on the chip, the safety pin is separated from the first groove and the second groove of the cover body in turn, the distance between the shell and the first base is greater than zero, the liquid channel in the first base and the liquid cavity in the shell are not connected, and then the chip is placed on the carrier on the lower side of the fixed plate, and the two V-shaped grooves of the carrier support the two V-shaped detection cavities of the storage device, such as Figure 2 As shown;

[0116] (A2) pulling the fixing plate forward, and the fixing plate moves forward along the guide rail;

[0117] The transmission member passing through the fixed plate moves forward in a direction parallel to the extension direction of the guide rail, and the bottom end of the transmission member moves forward in the second groove, thereby being stuck in the first groove at the upper end of the linkage part of the multi-channel selector valve, so that the first motor drives the rotation of the linkage part through the transmission member. Figure 1 As shown;

[0118] (A3) The second motor rotates, driving the eccentric wheel to rotate against the bottom wall of the fifth through hole of the connecting plate, pushing the connecting plate downward, thereby pushing the moving member and the cover body downward, the downwardly moving shell and the fixed first base are buckled together, the second group of openings enter the outlet of the liquid cavity, puncturing the film, so that the liquid channel in the first base is connected to the liquid cavity in the shell;

[0119] The mixing stage comprises the following steps:

[0120] The first motor drives the linkage part, the rotating disk and the rotating member to rotate by using the transmission member, and uses the encoder to position so that the fourth opening on the rotating disk selectively communicates with the first group of openings; in this state, the liquid cavity is selectively connected with the second group of openings, the first group of openings, the fourth opening, the rotating disk and the channels and cavities in the rotating member, but due to the resistance of the narrow channel, the liquid cannot flow out of the liquid cavity to the first group of openings by gravity alone; at the same time, the liquid in the liquid cavity that is not connected with the fourth opening cannot flow out to the first group of openings by its own gravity alone;

[0121] When the air pump is working, the gas in the cavity is discharged from the third channel and the fifth opening in sequence, and the cavity becomes negative pressure; the liquid in the liquid cavity breaks through and passes through the second group of openings, the channels in the first base, the first group of openings, the fourth opening, the second channel and the channels in the rotating member in sequence and enters the cavity, thereby realizing that multiple liquids (samples and reagents) are selectively extracted from the liquid cavity and mixed in the cavity;

[0122] During the reaction of the mixed liquid in the cavity, the temperature control unit maintains the set temperature of the mixed liquid through the heater and the refrigerator;

[0123] When there are magnetic beads in the mixed liquid, the electromagnet is used to adsorb the magnetic beads, and the magnetic beads gather at the bottom of the cavity; the ultrasonic module is used to disperse the gathered magnetic beads.

[0124] The delivery phase includes the following steps:

[0125] The first motor drives the linkage part to rotate, and through the positioning of the encoder, the fourth opening on the rotating rotating disk is connected to the first opening;

[0126] Inject gas into the cavity through the air pump and the fifth opening, so that the liquid surface in the cavity is under positive pressure, and the mixed liquid in the cavity is pressed to pass through the hollow rotating member, the second channel, the fourth opening, the first opening, the first channel and the eighth opening in sequence, and then enter the detection cavity in the storage device through the ninth opening and the second opening;

[0127] The detection phase includes the following steps:

[0128] (B1) The third motor drives the rotating member to rotate, so that different groups of detection units correspond to the incident optical fiber and the output optical fiber respectively, and the excitation light of different wavelengths output by each emission module is respectively emitted into the detection cavity through the incident optical fiber, and the light emitted from the detection cavity is respectively transmitted to each detection module through the output optical fiber, and the parameters of the sample are obtained after analysis;

[0129] The temperature of the liquid in the detection chamber is increased by using an electric heating plate, and the temperature of the liquid in the detection chamber is lowered by using the airflow generated by the fan, so that the liquid in the detection chamber maintains a set temperature.

[0130] Embodiment 3:

[0131] The application example of the liquid detection system in Example 1 of the present invention in fluorescent quantitative PCR is different from that in Example 2 in that:

[0132] 1. The bottom openings of each liquid chamber are not distributed in concentric circles, and the first group of openings are also not distributed in concentric circles.

[0133] 2. An annular groove is arranged on the periphery of the second through hole, and the sealing member is located in the annular groove and is sleeved on the outside of the rotating member.

[0134] 3. The end of the horizontal part of the safety pin away from the end inserted into the first groove also has a vertical part. When the safety pin is inserted into the second groove, the vertical part is exposed, which is convenient for the operator to hold and pull out.

[0135] 4. The first switching module uses a combination of multiple reflectors to combine the excitation light beams of different wavelengths output by multiple emission modules. The combined light beams are coupled into the incident optical fiber, which is then incident into each detection cavity after beam splitting. The power of the light source is switched on and off to achieve the selective injection of light beams of different wavelengths into the detection cavity.

[0136] The output optical fibers corresponding to each detection cavity are combined and then divided into multiple beams by an optical beam splitter. The optical beam splitter adopts a combination of a reflector and a transflector, and the detection modules are placed on each optical path after the splitting. An optical switch is provided on the output optical fiber to realize the switching function. Compared with the switching in Example 2, no moving parts are used, which improves the operation stability and reliability.

[0137] In the above embodiment, the first matching portion adopts a groove and the second matching portion adopts a key. Of course, they can also be interchanged, that is, the first matching portion adopts a key and the second matching portion adopts a groove.

Claims

1. A liquid detection system, comprising a chip and a frame, wherein the chip comprises a shell, a cover, a first base and a multi-channel selector valve; the shell has a plurality of liquid chambers, the cover is buckled on the top of the shell, and the multi-channel selector valve is fixed on the first base and blocked by the first base; The linkage part of the multi-channel directional selector valve passes through the first base and the housing, and has a first matching part at the upper end; it is characterized in that: The liquid detection system further comprises: a carrier, which is arranged on the frame and is suitable for carrying the chip; A fixed plate, the fixed plate having a first through hole and being arranged on a guide rail through a sliding member; when the fixed plate is in the working position, the first through hole and the first matching portion correspond to each other up and down; a guide rail, the guide rail is horizontally arranged on the frame; a first motor, the first motor is fixed to the fixed plate; a transmission member, the transmission member is driven by the first motor and passes through the first through hole; when the fixed plate is in the working position, the second matching portion at the bottom end of the transmission member matches with the first matching portion and drives the linkage part to rotate.

2. The liquid detection system according to claim 1, characterized in that: The first matching portion adopts a first groove, and the second matching portion adopts a key.

3. The liquid detection system according to claim 2, characterized in that: In the horizontal direction, the width of the first groove increases; the bottom end of the transmission member is not lower than the bottom wall height of the first groove and is not higher than the top height of the linkage part; in the horizontal direction, the width from one end to the other end of the bottom end of the transmission member increases.

4. The liquid detection system according to claim 2, characterized in that: The cover body has a second groove extending in a direction parallel to the extension direction of the guide rail; when the fixing plate moves forward along the guide rail, the end of the transmission member moves forward in the second groove and cooperates with the linkage part.

5. The liquid detection system according to claim 4, characterized in that: The detection system also includes: a safety pin, which has a horizontal part; two side parts of the horizontal part that are opposite to each other and extend along the length direction respectively have strip-shaped protrusions extending outward, and the strip-shaped protrusions extend continuously or intermittently along the length direction; the two side parts have a first group of grooves suitable for the elastic part to be inserted into; the end of the horizontal part is suitable for being inserted into the first groove; the two opposite walls of the second groove respectively have wings that block the strip-shaped protrusion inserted into the second groove from moving upward, and the distance between the two wings is less than the width of the second groove; the two wings respectively have suspended elastic parts.

6. The liquid detection system according to claim 1, characterized in that: The second matching portion adopts a first groove, and the first matching portion adopts a key.

7. The liquid detection system according to claim 1, characterized in that: The first base has a second opening, a third opening and a third through hole suitable for the linkage part to pass through, the bottom end of the first base has a first opening and a first group of openings distributed in concentric circles around the third through hole, the upper end has a second group of openings, the second group of openings arranged above and below are connected to the first group of openings through mutually isolated channels in the first base respectively; the second group of openings is suitable for communicating with the liquid cavity; The first opening is connected with the second opening and the third opening through a first channel; the multi-channel selector valve also includes a rotating body, which has a linkage part passing through the third through hole, a rotating disk with a radius larger than the third through hole and located on the lower side of the first base, and a rotating member extending downward, which are arranged in sequence from top to bottom; the upper end of the rotating disk has a fourth opening which is selectively connected with the first group of openings and the first opening when it rotates, and the second channel of the rotating disk connects the fourth opening and the hollow rotating member; the chip also includes a second base and a storage device, the lower end of the second base has a cavity suitable for the rotating member to extend into, and the cavity is sealed with the rotating disk or the bottom end of the first base; the second base outside the cavity has a fifth opening connected to the cavity; the storage device has a detection cavity, and the second opening and the third opening are respectively connected to the detection cavity.

8. The liquid detection system according to claim 7, characterized in that: From bottom to top, the cross-sectional area of ​​the cavity gradually increases, and the distance between the bottom end of the rotating member and the bottom wall of the cavity is less than 1 mm.

9. The liquid detection system according to claim 7, characterized in that: The bottom end of the first base has a third groove surrounding the third through hole, and the first group of openings and the first opening are located in the third groove; the upper end of the second base has an annular protrusion suitable for being inserted into the third groove, and the rotating disk is located in the third groove.

10. The liquid detection system according to claim 9, characterized in that: The second base above the cavity has a second through hole suitable for the rotating member to pass through, and the periphery of the second through hole has a circular groove or an annular groove; the sealing member is arranged in the circular groove or the annular groove.

Images